Rh7DG187700

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0021260
Physical Location & Seq
Reverse (-)
17365282 .. 17365551
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG187700.1

Sequence Viewer

Length: 270 bp
ATGGCAAGTGGAACAGCGTCTGGCATGGCACCTTATGCTTACTTGGCAATGTACCAAGTTTGTTATGATCACGGTTGTTCTGAAAGTGACATTTTAGCTATTATGGACACTGCTGTTGCCGATGGAGTGGACGTGCTCTCCCTCTCACTTGGTGGTGGCTCAGCTGCTTTATATTATGATGGAATCACAGTTGGTGCATTTGGAGCAATTCAGAAGGGAGTTTTTGTCAGCTGCTCAGCAGGAAATGGTTTATGGTTCATTGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

89

Amino Acids

9.05

Weight (kDa)

4.05

Isoelectric Point (pI)

26.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 6 - 83 1.1e-09 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021260)

Species Orthologous Gene IDs
pyrus_communis pycom04g06460
rosa_chinensis RchiOBHm_Chr7g0200041 RchiOBHm_Chr7g0200131
rosa_samantha Rh7DG186600 Rh7DG187700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 1 cut(s) 53
AjiI CACGTC 1 cut(s) 133
AluBI AGCT 3 cut(s) 98, 164, 231
AluI AGCT 3 cut(s) 98, 164, 231
Alw21I GWGCWC 1 cut(s) 138
AlwNI CAGNNNCTG 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 164, 231
BanI GGYRCC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 151, 218
BccI CCATC 2 cut(s) 116, 173
BclI TGATCA 1 cut(s) 67
BisI GCNGC 2 cut(s) 165, 232
BlpI GCTNAGC 2 cut(s) 160, 235
BlsI GCNGC 2 cut(s) 166, 233
BmgBI CACGTC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 30
Bpu1102I GCTNAGC 2 cut(s) 160, 235
BsaXI ACNNNNNCTCC 6 cut(s) 117, 122, 147, 152, 210, 240
Bse3DI GCAATG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 174, 249
BseXI GCAGC 2 cut(s) 151, 218
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 67
Bsp1720I GCTNAGC 2 cut(s) 160, 235
BspCNI CTCAG 2 cut(s) 173, 248
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 54
BssMI GATC 1 cut(s) 67
Bst4CI ACNGT 2 cut(s) 74, 190
BstAPI GCANNNNNTGC 1 cut(s) 35
BstDEI CTNAG 2 cut(s) 160, 235
BstKTI GATC 1 cut(s) 70
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 3 cut(s) 35, 44, 203
BstV1I GCAGC 2 cut(s) 151, 218
BtrI CACGTC 1 cut(s) 133
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 20
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 52
CviAII CATG 1 cut(s) 25
CviJI RGCY 4 cut(s) 98, 159, 164, 231
CviKI_1 RGCY 4 cut(s) 98, 159, 164, 231
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 2 cut(s) 160, 235
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
DraIII CACNNNGTG 1 cut(s) 152
FaeI CATG 1 cut(s) 28
FaiI YATR 7 cut(s) 26, 36, 66, 104, 172, 177, 253
FatI CATG 1 cut(s) 24
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 165, 232
Fsp4HI GCNGC 2 cut(s) 165, 232
GluI GCNGC 2 cut(s) 165, 232
HgaI GACGC 1 cut(s) 6
Hin1II CATG 1 cut(s) 28
HinfI GANTC 1 cut(s) 183
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 82, 213
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 74, 190
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 44, 203
HpyF3I CTNAG 2 cut(s) 160, 235
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 2 cut(s) 6, 225
Lsp1109I GCAGC 2 cut(s) 151, 218
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 1 cut(s) 207
MnlI CCTC 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 164, 231
MwoI GCNNNNNNNGC 3 cut(s) 35, 44, 203
NdeII GATC 1 cut(s) 67
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 2 cut(s) 166, 233
PspN4I GGNNCC 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 164, 231
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SatI GCNGC 2 cut(s) 165, 232
Sau3AI GATC 1 cut(s) 67
SduI GDGCHC 1 cut(s) 138
SetI ASST 5 cut(s) 34, 100, 135, 166, 233
SgeI CNNG 9 cut(s) 18, 33, 37, 55, 68, 83, 145, 161, 252
Sse9I AATT 1 cut(s) 207
TaaI ACNGT 2 cut(s) 74, 190
TaiI ACGT 1 cut(s) 135
TasI AATT 1 cut(s) 207
TfiI GAWTC 1 cut(s) 183
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 2 cut(s) 164, 231
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 247
TspRI CASTG 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.