pycom04g12820

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0024415
Physical Location & Seq
Reverse (-)
15822367 .. 15822840
474 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
ATGTCACTCATTCCTAGTAACATCTTTGTTGGTCACTACGATCCTTTCTGCCACAATGTTTGGGACCCATTTGAGGAATTTCATTATGGAGGCCCAATGATCATCCCACAGCATGCTTTCCCAAGTGAAGCAACCTCCTTCGTGACCTCAATGACCGACTGGAAGGAGACCTCAGATGGGAACTTAGCACTCAAGGTACATCTTCCGGGGTTCAAGAGTGAAGAAGTGAAGGTGGATGTGGAGGAAGGTAGGGTTCTTAGTATTAGGGGCGAGAAAAAGGTAGAGAGAGAAGAAAATCACGACGGTTGGCACCGCTTTGAGAGGAGCAGTGGCAAGTTCATTAGGCGTTTGAGGTTGCCTGAGAATGCCAAGGCCGAGAAGATGAAGGTGTTCGTGGAAAATGGAATGCTCACTGTCACGGTTCCTAAGGAGAATGAGGTTATTAAGTACTCTCCTCATACCAGAGTGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

158

Amino Acids

18.16

Weight (kDa)

6.31

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 58 - 149 1.4e-21 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024415)

Species Orthologous Gene IDs
malus_domestica MD04G1140600.v1.1
prunus_persica Prupe.6G266500_v2.0.a1
pyrus_communis pycom04g12820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AciI CCGC 1 cut(s) 313
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 2 cut(s) 198, 449
AfiI CCNNNNNNNGG 2 cut(s) 73, 177
AgsI TTSAA 1 cut(s) 214
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 1 cut(s) 161
Alw44I GTGCAC 1 cut(s) 467
AlwI GGATC 1 cut(s) 35
AoxI GGCC 2 cut(s) 91, 372
ApaLI GTGCAC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 64, 92
AsuC2I CCSGG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 471
BccI CCATC 1 cut(s) 170
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 15, 472
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 92
BmiI GGNNCC 4 cut(s) 65, 66, 311, 423
BmrFI CCNGG 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 206, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 177
Bse1I ACTGG 1 cut(s) 164
Bse21I CCTNAGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 206, 369
BseGI GGATG 2 cut(s) 102, 241
BseLI CCNNNNNNNGG 2 cut(s) 73, 177
BseMII CTCAG 2 cut(s) 186, 351
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 2 cut(s) 337, 444
BseSI GKGCMC 1 cut(s) 471
BshFI GGCC 2 cut(s) 93, 374
BshNI GGYRCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 73, 177
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 2 cut(s) 370, 411
BsnI GGCC 2 cut(s) 93, 374
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 40, 99
BspACI CCGC 1 cut(s) 313
BspANI GGCC 2 cut(s) 93, 374
BspCNI CTCAG 2 cut(s) 185, 352
BspLI GGNNCC 4 cut(s) 65, 66, 311, 423
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 206, 369
BssMI GATC 2 cut(s) 40, 99
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 305, 415, 421
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 5 cut(s) 172, 184, 257, 360, 426
BstF5I GGATG 2 cut(s) 102, 241
BstKTI GATC 2 cut(s) 43, 102
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 40, 99
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 471
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 2 cut(s) 93, 374
BtsCI GGATG 2 cut(s) 102, 241
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 334, 411
Cac8I GCNNGC 1 cut(s) 114
Cfr13I GGNCC 2 cut(s) 64, 92
Csp6I GTAC 2 cut(s) 197, 448
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 1 cut(s) 113
CviJI RGCY 2 cut(s) 93, 374
CviKI_1 RGCY 2 cut(s) 93, 374
CviQI GTAC 2 cut(s) 197, 448
DdeI CTNAG 5 cut(s) 172, 184, 257, 360, 426
DpnI GATC 2 cut(s) 42, 101
DpnII GATC 2 cut(s) 40, 99
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 1 cut(s) 116
FaiI YATR 3 cut(s) 87, 114, 459
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 112
FbaI TGATCA 1 cut(s) 99
FokI GGATG 2 cut(s) 89, 248
FspBI CTAG 2 cut(s) 15, 472
HaeIII GGCC 2 cut(s) 93, 374
HapII CCGG 1 cut(s) 206
Hin1II CATG 1 cut(s) 116
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 3 cut(s) 142, 214, 299
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 5 cut(s) 148, 157, 223, 239, 379
HpyCH4III ACNGT 3 cut(s) 305, 415, 421
HpyCH4V TGCA 1 cut(s) 469
HpyF3I CTNAG 5 cut(s) 172, 184, 257, 360, 426
Hsp92II CATG 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 2 cut(s) 40, 99
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 3 cut(s) 145, 219, 372
MaeI CTAG 2 cut(s) 15, 472
MaeIII GTNAC 5 cut(s) 3, 17, 32, 142, 415
MalI GATC 2 cut(s) 42, 101
MboI GATC 2 cut(s) 40, 99
MboII GAAGA 4 cut(s) 194, 233, 302, 391
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 1 cut(s) 444
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 2 cut(s) 370, 411
NciI CCSGG 1 cut(s) 207
NdeII GATC 2 cut(s) 40, 99
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 65, 66, 311, 423
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 4 cut(s) 3, 32, 142, 415
NspI RCATGY 1 cut(s) 116
PaeI GCATGC 1 cut(s) 116
PctI GAATGC 2 cut(s) 370, 411
PdmI GAANNNNTTC 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
PspN4I GGNNCC 4 cut(s) 65, 66, 311, 423
PspPI GGNCC 2 cut(s) 64, 92
PspPPI RGGWCCY 1 cut(s) 64
RsaI GTAC 2 cut(s) 198, 449
RsaNI GTAC 2 cut(s) 197, 448
SaqAI TTAA 1 cut(s) 444
Sau3AI GATC 2 cut(s) 40, 99
Sau96I GGNCC 2 cut(s) 64, 92
ScaI AGTACT 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 471
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SphI GCATGC 1 cut(s) 116
Sse9I AATT 1 cut(s) 77
SsiI CCGC 1 cut(s) 313
SspMI CTAG 2 cut(s) 15, 472
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 305, 415, 421
TaqII GACCGA 1 cut(s) 170
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 447
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 334, 418
TseFI GTSAC 4 cut(s) 3, 32, 142, 415
Tsp45I GTSAC 4 cut(s) 3, 32, 142, 415
TspDTI ATGAA 3 cut(s) 71, 328, 398
TspRI CASTG 2 cut(s) 334, 418
VneI GTGCAC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 2 cut(s) 15, 472
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.