pycom04g14580

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0024633
Physical Location & Seq
Forward (+)
17448723 .. 17450022
1300 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 393 bp
ATGACAAAGCTGATAGAGAAGGCTAGAAACAATGCATCAGCTTATGAGAACCCTTCAGAATACTGTGACCGCGATATGGCAAAGAGTGATCGAACTTTTTATTGTATGGCAACACAAATCGATCCCCTGAGGACATATCCGTACAGATATAGGGCCGCAGTTTTGATGGATGACCACAAAGAAGGAGAAGCCATTGAAGAGCTAACAAAGGCCATAACTTTCAAGCCAGACCTGCAGTTGCTACATCTTCGAGCAGCATTCCACGAGTCAATGGGTGACTTTGTCTCCACCGTCCGAGACTGTGAAGCAGCCCTCTGTCTTGATCCCAACCATGCTGACACTCATGACCTCTATGCTAAATCACGGGAACGTGTCAATGAACAACAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

131

Amino Acids

15.07

Weight (kDa)

5.51

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024633)

Species Orthologous Gene IDs
pyrus_communis pycom04g14580
rosa_multiflora Rmu_co8296871.1_g000001 Rmu_sc0020622.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 240
AccII CGCG 1 cut(s) 72
AciI CCGC 2 cut(s) 70, 156
AclWI GGATC 2 cut(s) 116, 317
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 197, 223
AluBI AGCT 3 cut(s) 10, 41, 202
AluI AGCT 3 cut(s) 10, 41, 202
Alw26I GTCTC 2 cut(s) 289, 291
AlwI GGATC 2 cut(s) 116, 317
AoxI GGCC 2 cut(s) 153, 210
ApeKI GCWGC 2 cut(s) 254, 308
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 128
BauI CACGAG 1 cut(s) 263
BbvI GCAGC 2 cut(s) 266, 320
BccI CCATC 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 289, 291
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 240
BisI GCNGC 3 cut(s) 156, 255, 309
BlsI GCNGC 3 cut(s) 157, 256, 310
BmgT120I GGNCC 1 cut(s) 153
BmsI GCATC 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse21I CCTNAGG 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 120
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 266, 320
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 155, 212
BshVI ATCGAT 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 2 cut(s) 289, 291
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 2 cut(s) 155, 212
Bsp143I GATC 3 cut(s) 88, 121, 322
BspACI CCGC 2 cut(s) 70, 156
BspANI GGCC 2 cut(s) 155, 212
BspCNI CTCAG 1 cut(s) 120
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 1 cut(s) 72
BspHI TCATGA 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 2 cut(s) 116, 317
BspQI GCTCTTC 1 cut(s) 192
BssMI GATC 3 cut(s) 88, 121, 322
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 3 cut(s) 65, 292, 302
Bst6I CTCTTC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 72
BstKTI GATC 3 cut(s) 91, 124, 325
BstMAI GTCTC 2 cut(s) 289, 291
BstMBI GATC 3 cut(s) 88, 121, 322
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstSFI CTRYAG 1 cut(s) 233
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 2 cut(s) 266, 320
Bsu15I ATCGAT 1 cut(s) 120
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 2 cut(s) 155, 212
BsuTUI ATCGAT 1 cut(s) 120
BtsCI GGATG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 240
CciI TCATGA 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 332, 344
CviJI RGCY 9 cut(s) 10, 23, 41, 155, 191, 202, 212, 226, 311
CviKI_1 RGCY 9 cut(s) 10, 23, 41, 155, 191, 202, 212, 226, 311
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 1 cut(s) 128
DpnI GATC 3 cut(s) 90, 123, 324
DpnII GATC 3 cut(s) 88, 121, 322
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 39
Eco81I CCTNAGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 37
FaeI CATG 2 cut(s) 335, 347
FaiI YATR 9 cut(s) 45, 77, 107, 136, 150, 215, 333, 345, 354
FatI CATG 2 cut(s) 331, 343
Fnu4HI GCNGC 3 cut(s) 156, 255, 309
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 3 cut(s) 156, 255, 309
FspBI CTAG 1 cut(s) 24
GluI GCNGC 3 cut(s) 156, 255, 309
HaeIII GGCC 2 cut(s) 155, 212
Hin1II CATG 2 cut(s) 335, 347
HinfI GANTC 1 cut(s) 266
HphI GGTGA 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 58, 296
Hpy188III TCNNGA 2 cut(s) 320, 344
HpyAV CCTTC 3 cut(s) 13, 63, 176
HpyCH4III ACNGT 3 cut(s) 65, 292, 302
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 2 cut(s) 35, 235
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 2 cut(s) 335, 347
Kzo9I GATC 3 cut(s) 88, 121, 322
LguI GCTCTTC 1 cut(s) 192
LpnPI CCDG 3 cut(s) 140, 240, 245
Lsp1109I GCAGC 2 cut(s) 266, 320
LweI GCATC 1 cut(s) 44
MaeI CTAG 1 cut(s) 24
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 2 cut(s) 65, 275
MalI GATC 3 cut(s) 90, 123, 324
MboI GATC 3 cut(s) 88, 121, 322
MboII GAAGA 2 cut(s) 209, 239
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 3 cut(s) 123, 323, 359
Mph1103I ATGCAT 1 cut(s) 37
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 232
NdeII GATC 3 cut(s) 88, 121, 322
NlaIII CATG 2 cut(s) 335, 347
NmuCI GTSAC 2 cut(s) 65, 275
NsiI ATGCAT 1 cut(s) 37
PagI TCATGA 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 192
PctI GAATGC 1 cut(s) 257
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 3 cut(s) 157, 256, 310
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PspPI GGNCC 1 cut(s) 153
PstI CTGCAG 1 cut(s) 237
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 192
SatI GCNGC 3 cut(s) 156, 255, 309
Sau3AI GATC 3 cut(s) 88, 121, 322
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 275
SetI ASST 6 cut(s) 12, 43, 204, 234, 351, 373
SfaNI GCATC 1 cut(s) 44
SfcI CTRYAG 1 cut(s) 233
SsiI CCGC 2 cut(s) 70, 156
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 3 cut(s) 65, 292, 302
TaiI ACGT 1 cut(s) 373
TaqI TCGA 3 cut(s) 91, 120, 250
TauI GCSGC 1 cut(s) 158
TseFI GTSAC 2 cut(s) 65, 275
TseI GCWGC 2 cut(s) 254, 308
Tsp45I GTSAC 2 cut(s) 65, 275
TspDTI ATGAA 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 129
Tth111I GACNNNGTC 1 cut(s) 281
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.