pycom04g20200

cell redox homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0028742
Physical Location & Seq
Reverse (-)
21737342 .. 21740168
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20200.3

Sequence Viewer

Length: 867 bp
ATGCCAACAACCACCGCAGCAGCAGTATCTGCCATTCCTTCCTCCAAGGCATCCGCCTCCTCCTTGGCCTCGCTCCCAGCCAGGGTCTCCGCCTCCGGTGCACTCTCGGCCTCGGCCTTAGGCCCAGGCTCAAAACTTCTGTTTCCGAGTCCCCAAGACATGATCATGTTGACGGAAAAGAAGGAATATAAGAGAGCAATTAAAGAAGTTAAAGGTGGACGTTTGCCAGCAGTTTTCTACTTTACTGCAGCCGCTTGCCCAAATACCGTCACGTACTCAATTCCAATCGTTCGAAAGATCAGGGAGCATTTCCCACATGTAAAACTATATAAGGTTCTGATTACTGTTGAGAGCGACTTGCGCCCAATAGCATATGATTTGGCTGTTTGCATTTTTCCAACATTCCTTTTCTATCAAAACGGGGTTAAGGTTGATGAGATTGTTGGTGGTCATGCTGATCCCGTGATGGATATGTGTGAAAAACTTTTCAGGTACATCAGGCCAGTTGAAACCCCTCAGAGGACGAGGTCATCCTGGAAGGGGAAAACGGATACTCAACAACTTGTCCTACAAGCTAAGAAAGATGAGAATGACGTAGTAGTTGCAGGAGGGTGGAAAGTGGGAGATGGTTCGATGTTCTTCCAATGGAAAGGTGGTGCGGTAGCTGTTGATAGTAGTGACGTACCTTCAGTTAGTGAAGTGATGAAGGATGTTGAATCTTTATGTACAGCACCAAGGGGTTTCAGTATGTGGTGTTTTGCGAAGGGACTATTTACAGCTAAGCCTGAGAAGAGAGTGGTATTTTCAAACATTAAAGATCCTGGGCTCAAGATTGGTTGGTTTCGTGAAGAAATGGAGGTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko05418 map04621 map05418
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

289

Amino Acids

31.58

Weight (kDa)

9.07

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028742)

Species Orthologous Gene IDs
malus_domestica MD00G1175500.v1.1
pyrus_communis pycom04g20200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 15, 54, 90, 252, 659
AclWI GGATC 2 cut(s) 452, 812
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 4 cut(s) 275, 494, 684, 727
AfiI CCNNNNNNNGG 3 cut(s) 82, 519, 540
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 3 cut(s) 509, 716, 807
AjnI CCWGG 4 cut(s) 80, 124, 533, 820
AluBI AGCT 3 cut(s) 575, 665, 779
AluI AGCT 3 cut(s) 575, 665, 779
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 91
Alw44I GTGCAC 1 cut(s) 99
AlwI GGATC 2 cut(s) 452, 812
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 5 cut(s) 66, 108, 114, 121, 500
ApaLI GTGCAC 1 cut(s) 99
ApeKI GCWGC 3 cut(s) 17, 20, 248
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 292
AxyI CCTNAGG 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 103
BanII GRGCYC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 29, 32, 260
BccI CCATC 2 cut(s) 460, 620
BciT130I CCWGG 4 cut(s) 82, 126, 535, 822
BciVI GTATCC 1 cut(s) 544
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 91
BfmI CTRYAG 1 cut(s) 246
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 4 cut(s) 18, 21, 249, 252
BlpI GCTNAGC 1 cut(s) 780
BlsI GCNGC 4 cut(s) 19, 22, 250, 253
Bme1390I CCNGG 4 cut(s) 82, 126, 535, 822
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 4 cut(s) 82, 126, 535, 822
BmsI GCATC 1 cut(s) 59
Bpu1102I GCTNAGC 1 cut(s) 780
Bpu14I TTCGAA 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 812
BsaAI YACGTR 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 7 cut(s) 45, 63, 81, 111, 124, 734, 821
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 519, 540
Bse1I ACTGG 1 cut(s) 503
Bse21I CCTNAGG 1 cut(s) 118
BseBI CCWGG 4 cut(s) 82, 126, 535, 822
BseDI CCNNGG 7 cut(s) 45, 63, 81, 111, 124, 734, 821
BseGI GGATG 3 cut(s) 50, 530, 715
BseLI CCNNNNNNNGG 3 cut(s) 82, 519, 540
BseMII CTCAG 2 cut(s) 530, 777
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 3 cut(s) 29, 32, 260
BseYI CCCAGC 1 cut(s) 76
BshFI GGCC 5 cut(s) 68, 110, 116, 123, 502
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 135, 780
BslI CCNNNNNNNGG 3 cut(s) 82, 519, 540
BsmAI GTCTC 1 cut(s) 91
BsmFI GGGAC 2 cut(s) 135, 780
BsnI GGCC 5 cut(s) 68, 110, 116, 123, 502
Bso31I GGTCTC 1 cut(s) 91
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 103, 828
Bsp1407I TGTACA 1 cut(s) 725
Bsp143I GATC 4 cut(s) 162, 297, 457, 817
Bsp1720I GCTNAGC 1 cut(s) 780
BspACI CCGC 5 cut(s) 15, 54, 90, 252, 659
BspANI GGCC 5 cut(s) 68, 110, 116, 123, 502
BspCNI CTCAG 2 cut(s) 529, 778
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 2 cut(s) 452, 812
BspT104I TTCGAA 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 725
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 7 cut(s) 45, 63, 81, 111, 124, 734, 821
BssMI GATC 4 cut(s) 162, 297, 457, 817
BssT1I CCWWGG 3 cut(s) 45, 63, 734
Bst2UI CCWGG 4 cut(s) 82, 126, 535, 822
Bst4CI ACNGT 2 cut(s) 268, 346
Bst6I CTCTTC 1 cut(s) 785
BstAPI GCANNNNNTGC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 273
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 228, 256
BstDEI CTNAG 5 cut(s) 118, 516, 576, 780, 786
BstF5I GGATG 3 cut(s) 50, 530, 715
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 4 cut(s) 165, 300, 460, 820
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 4 cut(s) 162, 297, 457, 817
BstMWI GCNNNNNNNGC 4 cut(s) 29, 98, 107, 360
BstNI CCWGG 4 cut(s) 82, 126, 535, 822
BstNSI RCATGY 1 cut(s) 320
BstSCI CCNGG 4 cut(s) 80, 124, 533, 820
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 3 cut(s) 29, 32, 260
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
Bsu36I CCTNAGG 1 cut(s) 118
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 5 cut(s) 68, 110, 116, 123, 502
BtsCI GGATG 3 cut(s) 50, 530, 715
Cac8I GCNNGC 2 cut(s) 228, 256
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 274, 493, 683, 726
CviAII CATG 4 cut(s) 160, 166, 317, 452
CviQI GTAC 4 cut(s) 274, 493, 683, 726
DdeI CTNAG 5 cut(s) 118, 516, 576, 780, 786
DpnI GATC 4 cut(s) 164, 299, 459, 819
DpnII GATC 4 cut(s) 162, 297, 457, 817
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
EciI GGCGGA 2 cut(s) 43, 79
Eco130I CCWWGG 3 cut(s) 45, 63, 734
Eco24I GRGCYC 1 cut(s) 828
Eco31I GGTCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 672
Eco81I CCTNAGG 1 cut(s) 118
EcoRII CCWGG 4 cut(s) 80, 124, 533, 820
EcoT14I CCWWGG 3 cut(s) 45, 63, 734
EcoT38I GRGCYC 1 cut(s) 828
ErhI CCWWGG 3 cut(s) 45, 63, 734
FaeI CATG 4 cut(s) 163, 169, 320, 455
FaqI GGGAC 2 cut(s) 135, 780
FatI CATG 4 cut(s) 159, 165, 316, 451
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 18, 21, 249, 252
FokI GGATG 3 cut(s) 37, 517, 722
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 4 cut(s) 18, 21, 249, 252
GlaI GCGC 1 cut(s) 362
GluI GCNGC 4 cut(s) 18, 21, 249, 252
GsaI CCCAGC 1 cut(s) 80
HaeIII GGCC 5 cut(s) 68, 110, 116, 123, 502
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 363
Hin1II CATG 4 cut(s) 163, 169, 320, 455
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 2 cut(s) 148, 716
HpaII CCGG 1 cut(s) 96
Hpy166II GTNNAC 3 cut(s) 101, 171, 218
Hpy188I TCNGA 3 cut(s) 147, 339, 519
Hpy188III TCNNGA 2 cut(s) 829, 845
Hpy8I GTNNAC 3 cut(s) 101, 171, 218
HpyAV CCTTC 6 cut(s) 48, 175, 532, 696, 700, 757
HpyCH4III ACNGT 2 cut(s) 268, 346
HpyCH4IV ACGT 4 cut(s) 220, 272, 594, 681
HpyCH4V TGCA 4 cut(s) 101, 248, 390, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 98, 107, 360
HpyF3I CTNAG 5 cut(s) 118, 516, 576, 780, 786
HpySE526I ACGT 4 cut(s) 220, 272, 594, 681
Hsp92II CATG 4 cut(s) 163, 169, 320, 455
HspAI GCGC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 4 cut(s) 162, 297, 457, 817
LmnI GCTCC 2 cut(s) 78, 304
Lsp1109I GCAGC 3 cut(s) 29, 32, 260
LweI GCATC 1 cut(s) 59
MaeII ACGT 4 cut(s) 220, 272, 594, 681
MaeIII GTNAC 2 cut(s) 268, 677
MalI GATC 4 cut(s) 164, 299, 459, 819
MboI GATC 4 cut(s) 162, 297, 457, 817
MboII GAAGA 3 cut(s) 631, 802, 860
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 2 cut(s) 103, 828
MluCI AATT 2 cut(s) 198, 279
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 422
MseI TTAA 4 cut(s) 201, 210, 426, 813
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 4 cut(s) 82, 126, 535, 822
MvaI CCWGG 4 cut(s) 82, 126, 535, 822
MwoI GCNNNNNNNGC 4 cut(s) 29, 98, 107, 360
NdeI CATATG 1 cut(s) 373
NdeII GATC 4 cut(s) 162, 297, 457, 817
NlaIII CATG 4 cut(s) 163, 169, 320, 455
NmeAIII GCCGAG 2 cut(s) 86, 92
NmuCI GTSAC 2 cut(s) 268, 677
NspI RCATGY 1 cut(s) 320
NspV TTCGAA 1 cut(s) 292
PciI ACATGT 1 cut(s) 316
PfeI GAWTC 1 cut(s) 716
PflFI GACNNNGTC 1 cut(s) 526
PfoI TCCNGGA 1 cut(s) 533
PkrI GCNGC 4 cut(s) 19, 22, 250, 253
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 273
PscI ACATGT 1 cut(s) 316
Psp6I CCWGG 4 cut(s) 80, 124, 533, 820
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 4 cut(s) 80, 124, 533, 820
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 817
PsyI GACNNNGTC 1 cut(s) 526
RsaI GTAC 4 cut(s) 275, 494, 684, 727
RsaNI GTAC 4 cut(s) 274, 493, 683, 726
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 4 cut(s) 201, 210, 426, 813
SatI GCNGC 4 cut(s) 18, 21, 249, 252
Sau3AI GATC 4 cut(s) 162, 297, 457, 817
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 4 cut(s) 82, 126, 535, 822
SduI GDGCHC 2 cut(s) 103, 828
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 2 cut(s) 198, 279
SsiI CCGC 5 cut(s) 15, 54, 90, 252, 659
StyD4I CCNGG 4 cut(s) 80, 124, 533, 820
StyI CCWWGG 3 cut(s) 45, 63, 734
TaaI ACNGT 2 cut(s) 268, 346
TaiI ACGT 4 cut(s) 223, 275, 597, 684
TaqI TCGA 2 cut(s) 292, 632
TasI AATT 2 cut(s) 198, 279
TatI WGTACW 1 cut(s) 725
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 4 cut(s) 201, 210, 426, 813
Tru9I TTAA 4 cut(s) 201, 210, 426, 813
TseFI GTSAC 2 cut(s) 268, 677
TseI GCWGC 3 cut(s) 17, 20, 248
Tsp45I GTSAC 2 cut(s) 268, 677
TspDTI ATGAA 1 cut(s) 719
TspGWI ACGGA 2 cut(s) 188, 563
Tth111I GACNNNGTC 1 cut(s) 526
VneI GTGCAC 1 cut(s) 99
XceI RCATGY 1 cut(s) 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.