pycom05g08840

glycerol kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0024491
Physical Location & Seq
Forward (+)
11602229 .. 11603797
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGTGACCACAATGCCGCCGTTATTTTCTATTTCAGCAACCTTTAGCACTGACTCATCCTACGCTCACAGTCATAAACCTGTTTTGAGCACCAAGGAAGCTACAACAATGGCGTATGTTTTGGGTTTCCCTCTCAGGAACGTAGGAGTTACTAAATCTGTAGGTCTCTCTCATACGAACACCACGAAGCATCGACATGAGGTATTGAGATCCTTGATTTGCGCATCCAAAACTGGGAAAGCCAAATGGCATTGGTTTTTAAATAGTTTACTTTCGTGTTATACTAACGGCAAGTTAGGTACGAATGATGTTTATCTGAATGAGATTAATGGGTGGGTCAGCATGTATGAGGTTGGCAAATTGGTGATGGTTTTGAATGCAGAGAAAGTGTTTGAGGAAATAGCTAAGAGACAAAGCGAGGACGCTGCAGGAAACCCCTTCGATCGAATGACCCTTCTTTTTCATAATATGGTTGCATGCTCTCAGTTTTGCAAAACTATGGGTGAATTCTGGAATGGATGGGTTCCAGGAGGGGGATTAGGTGATCGCATGTCCTTTATTGATGCCGGCAAGACTGAGGAACTATTTCTACCATTAAGGGATGATGAAAAATTGTGTACCAATATATGTGTTAGTAAGAGAAGTTTTGGTATGGATGTAGCTTGTGTTGCTGCACCAATCTTGTTGTTTATCAAAGATCTATTGCAAGAAATCAACCTTTCAGATTTTACTGCAGGAAGGAAGATCAAACAGCTGAATGAGAAAGAGATTTCTATTATGATCATTACCGAAGGACAAGCAAACTATGAAGATTTTTCTGATTGTGTCAAAAATGGTGATGAGCGAACGTTTGAGCTGTTGCTCTACTTCAAAGCTATCAAGGCAGAGTGGCTTAACAGGGTTACAAAAATAACCAATTTTGGTAGTTTTATGGTACAATTGGATAGAAGTGTTGAAGCTTTGAATAAAGGCAAAAAGCAAAAAACTGGAGAGGGTGGATATTGTGATGAGATGAAGAGTTTGCATGAATTAAGCATGAAAAAGGCTGTGGATATGGCGACTGGGTTCGAAAGAGCATTGGCAGGACATGTTGTTAACAATATAATTTGTAGTACCACTGTAAGATTGGAGAGACTTCACTCAGAGGGGATTCATAGATTGCCAGAAGGGTGGTGTTCTGCGATATATCAACTGCTGATGATTGGTAAATCCATCATATCTAGTTCAAACAAAACTCAGGAAGCAAATGGGATGTCGTTTTGGGAGCTGGTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko01100 ko01110 ko01120 ko01130 ko01200 map00030 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
FGGY_C FGGY_N

Protein Analysis

426

Amino Acids

47.58

Weight (kDa)

7.56

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 299 - 416 2.5e-30 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024491)

Species Orthologous Gene IDs
malus_domestica MD11G1195900.v1.1
pyrus_communis pycom03g15140 pycom05g08840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 223
AciI CCGC 1 cut(s) 17
AclI AACGTT 1 cut(s) 848
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 506
AfaI GTAC 4 cut(s) 301, 619, 936, 1114
AfiI CCNNNNNNNGG 2 cut(s) 234, 533
AflIII ACRYGT 1 cut(s) 1087
AgsI TTSAA 5 cut(s) 376, 871, 956, 964, 1227
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 8 cut(s) 101, 404, 662, 754, 856, 875, 959, 1267
AluI AGCT 8 cut(s) 101, 404, 662, 754, 856, 875, 959, 1267
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 3 cut(s) 170, 403, 1126
AlwI GGATC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 425, 671
ApoI RAATTY 1 cut(s) 506
ArsI GACNNNNNNTTYG 2 cut(s) 1238, 1270
AseI ATTAAT 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 1 cut(s) 224
AsuHPI GGTGA 5 cut(s) 16, 376, 515, 554, 848
AsuII TTCGAA 1 cut(s) 1068
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 412, 658
BccI CCATC 3 cut(s) 361, 513, 1220
BceAI ACGGC 2 cut(s) 4, 304
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 1 cut(s) 780
BcoDI GTCTC 3 cut(s) 170, 403, 1126
BfaI CTAG 1 cut(s) 1221
BfmI CTRYAG 3 cut(s) 159, 426, 732
BglII AGATCT 1 cut(s) 697
BisI GCNGC 3 cut(s) 17, 426, 672
BlsI GCNGC 3 cut(s) 18, 427, 673
Bme1390I CCNGG 1 cut(s) 528
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 528
BmrI ACTGGG 2 cut(s) 243, 1071
BmsI GCATC 3 cut(s) 199, 233, 553
BmuI ACTGGG 2 cut(s) 243, 1071
BpmI CTGGAG 1 cut(s) 1008
Bpu14I TTCGAA 1 cut(s) 1068
BsaBI GATNNNNATC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 533
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 3 cut(s) 238, 991, 1066
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 6 cut(s) 56, 224, 524, 607, 661, 1257
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 234, 533
BseMII CTCAG 5 cut(s) 148, 497, 567, 1155, 1250
BseNI ACTGG 3 cut(s) 238, 991, 1066
BseXI GCAGC 2 cut(s) 412, 658
BsgI GTGCAG 1 cut(s) 657
Bsh1285I CGRYCG 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 567
BslI CCNNNNNNNGG 2 cut(s) 234, 533
BsmAI GTCTC 3 cut(s) 170, 403, 1126
BsmI GAATGC 1 cut(s) 382
Bso31I GGTCTC 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 1068
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
BspACI CCGC 1 cut(s) 17
BspCNI CTCAG 5 cut(s) 147, 496, 568, 1154, 1249
BspLI GGNNCC 1 cut(s) 525
BspMAI CTGCAG 2 cut(s) 430, 736
BspPI GGATC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 1068
BspTNI GGTCTC 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 3 cut(s) 238, 991, 1066
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 71, 1120
Bst6I CTCTTC 1 cut(s) 1010
BstBI TTCGAA 1 cut(s) 1068
BstC8I GCNNGC 2 cut(s) 478, 568
BstDEI CTNAG 6 cut(s) 134, 405, 483, 576, 1141, 1236
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 6 cut(s) 56, 224, 524, 607, 661, 1257
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 7 cut(s) 212, 445, 547, 700, 747, 783, 1275
BstMAI GTCTC 3 cut(s) 170, 403, 1126
BstMBI GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 2 cut(s) 668, 881
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 4 cut(s) 346, 480, 553, 1091
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 3 cut(s) 159, 426, 732
BstV1I GCAGC 2 cut(s) 412, 658
BstX2I RGATCY 2 cut(s) 209, 697
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 2 cut(s) 209, 697
BtsCI GGATG 6 cut(s) 56, 224, 524, 607, 661, 1257
BtsIMutI CAGTG 2 cut(s) 48, 1116
Cac8I GCNNGC 2 cut(s) 478, 568
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 566
CseI GACGC 1 cut(s) 431
Csp6I GTAC 4 cut(s) 300, 618, 935, 1113
CviAII CATG 7 cut(s) 197, 343, 477, 550, 1025, 1036, 1088
CviQI GTAC 4 cut(s) 300, 618, 935, 1113
DdeI CTNAG 6 cut(s) 134, 405, 483, 576, 1141, 1236
DpnI GATC 7 cut(s) 211, 444, 546, 699, 746, 782, 1274
DpnII GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
DraI TTTAAA 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Eco130I CCWWGG 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 4
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 7 cut(s) 200, 346, 480, 553, 1028, 1039, 1091
FatI CATG 7 cut(s) 196, 342, 476, 549, 1024, 1035, 1087
FbaI TGATCA 1 cut(s) 780
Fnu4HI GCNGC 3 cut(s) 17, 426, 672
FokI GGATG 6 cut(s) 43, 211, 531, 614, 668, 1264
Fsp4HI GCNGC 3 cut(s) 17, 426, 672
FspBI CTAG 1 cut(s) 1221
FspI TGCGCA 1 cut(s) 223
GlaI GCGC 1 cut(s) 223
GluI GCNGC 3 cut(s) 17, 426, 672
GsuI CTGGAG 1 cut(s) 1008
HapII CCGG 1 cut(s) 567
HgaI GACGC 1 cut(s) 431
HhaI GCGC 1 cut(s) 224
Hin1II CATG 7 cut(s) 200, 346, 480, 553, 1028, 1039, 1091
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 2 cut(s) 53, 1150
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 1 cut(s) 567
HphI GGTGA 5 cut(s) 16, 376, 515, 554, 848
Hpy166II GTNNAC 3 cut(s) 269, 618, 1096
Hpy188I TCNGA 5 cut(s) 318, 724, 820, 1144, 1277
Hpy188III TCNNGA 3 cut(s) 136, 511, 1238
Hpy8I GTNNAC 3 cut(s) 269, 618, 1096
HpyAV CCTTC 5 cut(s) 448, 464, 732, 785, 1160
HpyCH4III ACNGT 2 cut(s) 71, 1120
HpyCH4IV ACGT 2 cut(s) 141, 848
HpyCH4V TGCA 8 cut(s) 380, 428, 476, 492, 674, 706, 734, 1024
HpyF10VI GCNNNNNNNGC 2 cut(s) 668, 881
HpyF3I CTNAG 6 cut(s) 134, 405, 483, 576, 1141, 1236
HpySE526I ACGT 2 cut(s) 141, 848
Hsp92II CATG 7 cut(s) 200, 346, 480, 553, 1028, 1039, 1091
HspAI GCGC 1 cut(s) 222
KroI GCCGGC 1 cut(s) 566
KroNI GCCGGC 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 780
KspAI GTTAAC 1 cut(s) 1096
Kzo9I GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
LmnI GCTCC 1 cut(s) 1264
Lsp1109I GCAGC 2 cut(s) 412, 658
LweI GCATC 3 cut(s) 199, 233, 553
MaeI CTAG 1 cut(s) 1221
MaeII ACGT 2 cut(s) 141, 848
MaeIII GTNAC 3 cut(s) 4, 148, 901
MalI GATC 7 cut(s) 211, 444, 546, 699, 746, 782, 1274
MboI GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
MboII GAAGA 3 cut(s) 754, 821, 1027
MfeI CAATTG 1 cut(s) 938
MflI RGATCY 2 cut(s) 209, 697
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 7 cut(s) 359, 506, 611, 916, 938, 1028, 1104
MlyI GAGTC 1 cut(s) 47
MnlI CCTC 9 cut(s) 141, 193, 343, 388, 412, 524, 571, 985, 1138
MroNI GCCGGC 1 cut(s) 566
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 6 cut(s) 260, 327, 596, 894, 1031, 1095
MslI CAYNNNNRTG 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 1 cut(s) 567
MspR9I CCNGG 1 cut(s) 528
MunI CAATTG 1 cut(s) 938
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 2 cut(s) 668, 881
NaeI GCCGGC 1 cut(s) 568
NdeII GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
NgoMIV GCCGGC 1 cut(s) 566
NlaIII CATG 7 cut(s) 200, 346, 480, 553, 1028, 1039, 1091
NlaIV GGNNCC 1 cut(s) 525
NmuCI GTSAC 1 cut(s) 4
NsbI TGCGCA 1 cut(s) 223
NspI RCATGY 4 cut(s) 346, 480, 553, 1091
NspV TTCGAA 1 cut(s) 1068
PaeI GCATGC 1 cut(s) 480
PciI ACATGT 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PctI GAATGC 1 cut(s) 382
PdiI GCCGGC 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 1150
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 3 cut(s) 18, 427, 673
Ple19I CGATCG 1 cut(s) 445
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PscI ACATGT 1 cut(s) 1087
PshBI ATTAAT 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 848
Psp6I CCWGG 1 cut(s) 526
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 525
PsrI GAACNNNNNNTAC 2 cut(s) 573, 605
PstI CTGCAG 2 cut(s) 430, 736
PsuI RGATCY 2 cut(s) 209, 697
PvuI CGATCG 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 754
RsaI GTAC 4 cut(s) 301, 619, 936, 1114
RsaNI GTAC 4 cut(s) 300, 618, 935, 1113
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 6 cut(s) 260, 327, 596, 894, 1031, 1095
SatI GCNGC 3 cut(s) 17, 426, 672
Sau3AI GATC 7 cut(s) 209, 442, 544, 697, 744, 780, 1272
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 3 cut(s) 199, 233, 553
SfcI CTRYAG 3 cut(s) 159, 426, 732
SfuI TTCGAA 1 cut(s) 1068
SmiMI CAYNNNNRTG 1 cut(s) 195
SphI GCATGC 1 cut(s) 480
Sse9I AATT 7 cut(s) 359, 506, 611, 916, 938, 1028, 1104
SsiI CCGC 1 cut(s) 17
SspMI CTAG 1 cut(s) 1221
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 71, 1120
TaiI ACGT 2 cut(s) 144, 851
TaqI TCGA 4 cut(s) 193, 441, 445, 1068
TasI AATT 7 cut(s) 359, 506, 611, 916, 938, 1028, 1104
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 1 cut(s) 1150
Tru1I TTAA 6 cut(s) 260, 327, 596, 894, 1031, 1095
Tru9I TTAA 6 cut(s) 260, 327, 596, 894, 1031, 1095
TscAI CASTG 2 cut(s) 55, 1123
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 425, 671
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 7 cut(s) 452, 621, 822, 1028, 1041, 1052, 1142
TspRI CASTG 2 cut(s) 55, 1123
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 506
XceI RCATGY 4 cut(s) 346, 480, 553, 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 1123
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.