pycom05g13880

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0024473
Physical Location & Seq
Forward (+)
17438177 .. 17439364
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGCATAGGACAGGATCGAAAACAAATGGTTATCTGCTTGTTCATGCCAATGGCGGATTGAATCAGATGAGAACAAGGATAATGAATGCTACCCTGGTTCTTCCACTGATGGACCATCAATCTTTTTGGACGGATCCAAGTGATTTTACAGACATTTTTGACTGGAGACACTTCATGAAAGTATTGAAAGACGATATTGATATTATCGAGTATTTGCCAACCAAATATGCAATTGCGAAGCCTGTTGTGAAGGCTCCCGTCTCCTGGTCGAAGGTTATAAAGTTTACGCACACTGATTCTCGGCTTGCTAACAATGGACTAGCATCCACCATCCAAAAGTTGAGGTGCCGTGTGAATTATGAGGCTCTCCAGTACGCGAAAGAGATTGAGGATCTTGGGAGAGTTTTGGTTGACAGACTGAGGAACAATAGCAATCTGTATATTGCTCTTCATTTGAGATATGAAAAAGACATGCTCGCTTTCATTGGTTGCAGCGACAACCTCACTGCTGAGGAATCTGAGGAGCTTCGGGTTATGAGGTACAGTGTCAAACATTGGAAGGAGAAGGATGTGATCGATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

195

Amino Acids

22.63

Weight (kDa)

8.78

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 11 - 183 3.7e-48 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024473)

Species Orthologous Gene IDs
malus_domestica MD07G1212500.v1.1
pyrus_communis pycom05g13880
rosa_multiflora Rmu_co8207126.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 345
AccII CGCG 1 cut(s) 377
AciI CCGC 1 cut(s) 54
AclWI GGATC 4 cut(s) 22, 128, 141, 399
AfaI GTAC 2 cut(s) 374, 542
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 2 cut(s) 61, 187
AjnI CCWGG 2 cut(s) 93, 263
AluBI AGCT 1 cut(s) 526
AluI AGCT 1 cut(s) 526
Alw26I GTCTC 2 cut(s) 160, 265
AlwI GGATC 4 cut(s) 22, 128, 141, 399
ApeKI GCWGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 112
AvaII GGWCC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 504
BccI CCATC 3 cut(s) 103, 123, 338
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 2 cut(s) 95, 265
BcoDI GTCTC 2 cut(s) 160, 265
BfaI CTAG 1 cut(s) 320
BisI GCNGC 1 cut(s) 493
BlsI GCNGC 1 cut(s) 494
Bme1390I CCNGG 2 cut(s) 95, 265
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 3 cut(s) 135, 255, 347
BmrFI CCNGG 2 cut(s) 95, 265
BmsI GCATC 1 cut(s) 332
BpmI CTGGAG 2 cut(s) 184, 353
Bpu10I CCTNAGC 1 cut(s) 510
Bsa29I ATCGAT 2 cut(s) 576, 580
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 2 cut(s) 167, 370
BseBI CCWGG 2 cut(s) 95, 265
BseCI ATCGAT 2 cut(s) 576, 580
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 3 cut(s) 323, 330, 574
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMII CTCAG 3 cut(s) 410, 501, 510
BseNI ACTGG 2 cut(s) 167, 370
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 580
BshNI GGYRCC 1 cut(s) 345
BshVI ATCGAT 2 cut(s) 576, 580
BsiEI CGRYCG 1 cut(s) 580
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 160, 265
BsmBI CGTCTC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 91
Bsp143I GATC 5 cut(s) 14, 133, 391, 573, 577
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 3 cut(s) 411, 502, 511
BspDI ATCGAT 2 cut(s) 576, 580
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 135, 255, 347
BspPI GGATC 4 cut(s) 22, 128, 141, 399
BspQI GCTCTTC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 345
BsrI ACTGG 2 cut(s) 167, 370
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 5 cut(s) 14, 133, 391, 573, 577
Bst2UI CCWGG 2 cut(s) 95, 265
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 306, 477
BstDEI CTNAG 3 cut(s) 419, 510, 519
BstF5I GGATG 3 cut(s) 323, 330, 574
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 5 cut(s) 17, 136, 394, 576, 580
BstMAI GTCTC 2 cut(s) 160, 265
BstMBI GATC 5 cut(s) 14, 133, 391, 573, 577
BstMCI CGRYCG 1 cut(s) 580
BstNI CCWGG 2 cut(s) 95, 265
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 93, 263
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 133, 391
BstYI RGATCY 2 cut(s) 133, 391
Bsu15I ATCGAT 2 cut(s) 576, 580
BsuTUI ATCGAT 2 cut(s) 576, 580
BtsCI GGATG 3 cut(s) 323, 330, 574
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 4 cut(s) 104, 291, 504, 550
Cac8I GCNNGC 2 cut(s) 306, 477
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 2 cut(s) 576, 580
Csp6I GTAC 2 cut(s) 373, 541
CviAII CATG 3 cut(s) 44, 175, 472
CviJI RGCY 5 cut(s) 241, 254, 304, 365, 526
CviKI_1 RGCY 5 cut(s) 241, 254, 304, 365, 526
CviQI GTAC 2 cut(s) 373, 541
DdeI CTNAG 3 cut(s) 419, 510, 519
DpnI GATC 5 cut(s) 16, 135, 393, 575, 579
DpnII GATC 5 cut(s) 14, 133, 391, 573, 577
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 93, 263
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 3 cut(s) 47, 178, 475
FatI CATG 3 cut(s) 43, 174, 471
Fnu4HI GCNGC 1 cut(s) 493
FokI GGATG 3 cut(s) 310, 317, 581
Fsp4HI GCNGC 1 cut(s) 493
FspBI CTAG 1 cut(s) 320
GluI GCNGC 1 cut(s) 493
GsuI CTGGAG 2 cut(s) 184, 353
Hin1II CATG 3 cut(s) 47, 178, 475
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 3 cut(s) 61, 296, 515
Hpy166II GTNNAC 2 cut(s) 285, 412
Hpy188I TCNGA 2 cut(s) 66, 520
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 2 cut(s) 285, 412
HpyAV CCTTC 4 cut(s) 244, 265, 553, 559
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4V TGCA 3 cut(s) 4, 230, 492
HpyF3I CTNAG 3 cut(s) 419, 510, 519
Hsp92II CATG 3 cut(s) 47, 178, 475
Kzo9I GATC 5 cut(s) 14, 133, 391, 573, 577
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 2 cut(s) 259, 523
LpnPI CCDG 7 cut(s) 80, 107, 148, 250, 255, 277, 383
Lsp1109I GCAGC 1 cut(s) 504
LweI GCATC 1 cut(s) 332
MaeI CTAG 1 cut(s) 320
MalI GATC 5 cut(s) 16, 135, 393, 575, 579
MboI GATC 5 cut(s) 14, 133, 391, 573, 577
MboII GAAGA 2 cut(s) 92, 440
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 2 cut(s) 133, 391
MluCI AATT 2 cut(s) 231, 355
MnlI CCTC 8 cut(s) 336, 355, 382, 414, 505, 512, 514, 531
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 95, 265
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 91
MvaI CCWGG 2 cut(s) 95, 265
MvnI CGCG 1 cut(s) 377
NdeII GATC 5 cut(s) 14, 133, 391, 573, 577
NlaIII CATG 3 cut(s) 47, 178, 475
NlaIV GGNNCC 3 cut(s) 135, 255, 347
NmeAIII GCCGAG 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 475
PagI TCATGA 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 3 cut(s) 61, 296, 515
PkrI GCNGC 1 cut(s) 494
Ple19I CGATCG 1 cut(s) 580
PsiI TTATAA 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 93, 263
PspGI CCWGG 2 cut(s) 93, 263
PspN4I GGNNCC 3 cut(s) 135, 255, 347
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 2 cut(s) 133, 391
PvuI CGATCG 1 cut(s) 580
RsaI GTAC 2 cut(s) 374, 542
RsaNI GTAC 2 cut(s) 373, 541
RseI CAYNNNNRTG 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 453
SatI GCNGC 1 cut(s) 493
Sau3AI GATC 5 cut(s) 14, 133, 391, 573, 577
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 95, 265
SetI ASST 5 cut(s) 276, 347, 504, 528, 542
SfaNI GCATC 1 cut(s) 332
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 48
Sse9I AATT 2 cut(s) 231, 355
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 93, 263
TaaI ACNGT 1 cut(s) 545
TaqI TCGA 5 cut(s) 17, 207, 269, 576, 580
TasI AATT 2 cut(s) 231, 355
TfiI GAWTC 3 cut(s) 61, 296, 515
TscAI CASTG 4 cut(s) 111, 298, 511, 550
TseI GCWGC 1 cut(s) 492
TspDTI ATGAA 7 cut(s) 32, 98, 163, 191, 440, 472, 477
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 4 cut(s) 111, 298, 511, 550
VpaK11BI GGWCC 1 cut(s) 112
XceI RCATGY 1 cut(s) 475
XspI CTAG 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.