pycom05g22260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0024642
Physical Location & Seq
Forward (+)
24668785 .. 24670848
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22260.11

Sequence Viewer

Length: 1554 bp
ATGTTGATCGACCTTGTGCAAAGAAATAAATCTCGTGGCCTTGTACGTATTACTAAAACCATTGGGTTCTGGTTGCATATGAGATTAATTAAGATCTTAGGTTCAATTCTCGTCAAAGGGAATTTTGAACCACATTATTGCTATCTCCAACCGAATGGGTTAAACATAGCACCATCTAGAATTATAATTCATATACCATTTCCAAACGAAGAGACAAAACATAGCCCTCTCAATAATTTATTAGTTTTAAAGTTTTCAAATCTAACCGTTAAATTTGAATCAATTATTGCAATTGAGAAGTTGAGCGCAAAAAAAGAGCTAAAAGGGGGGGCTATATGTAGTCAGACAGATGCTTCTTGGCCAAATAATGGTCTGAATTTTTTGGACACATTAGATCATTTTTTGGCTTTTAGACATTTAACATTTTCTATTGGAGATGACTTTATAAAATTTTATTTAAAACGAAAAAAATACAAGAGGACGAATTGCTATTTGCATCATATTATATTGGGCACTAATTTCCATCAAAGCAATCCTACTCTTCTCTCTCATCTCTTTCACCCTACCTCTCACCACATCACCACCGTTCATCAACCGCTTTATGCTTCTATCCACCTCCTCCCCCATCACCAAATCACTACCCTCTCTATAATCCAACCTAATCTCAATCGCCAATCCCAATTCCTTCACCATCTCAAAGGCATTCATTTGTTGCTCCGCGTAAATCGGCCACGTGGCAATCGGTACGCCGCACCACAAGCTCTCCAAAATCGAGTTCCAACCACAATGCGATATGAACCCTCCGATCGATCTGGTGGGCCAAGATCTTCGCTTGTGGGATCCACCCGCACACCAGGCCGAATTCACTCGTCCGCTCCAAAAACCCATTGGGCAACACATCTTCCACGTTCGCGTGGTCGCTTGGGAGATCCAATTGGGACTTGGGCGGCTCACGTAATGCCCATAGGAATCGGACCCTGGCACGTTCAAGCCCATATGCTATCTCCCGCACCTGAGGATGAGGGCTGGTCATCGAGCCACCTGATGACGCTTTCATATTGGTTTAAATCCCGCTTTTGGGTTGCCAACGAGTCAAGTGCATGCGATTCCAATTCTCTAAAGGTATTCACAACAATACCCTTTGTTTCGGTGTACCTTCGCGCATGGGATAGATACCACGAGTATGCATCTCCCTTCTTATTCAACACGGCTGTAGGCAAAACGAGTGGTGGCACCGAGTTGACAAAACCCGGTATGCTCAACTCGGTGCCCAAGTCAACGAACTCAGCGGGGTTTTGGGCATCCAAGAATGGTAAGTGGAGCATAAAGCCAAGTTCGTTAGCAACGTCAATCATGGAAGTACAAAACATGTCAACAAAAAACCCTACAACCTGGTCCGAGTTAGATGATTTTGACTCGGCCGAGTCGGATGACATGAGTTTGGTGATTGCATTCTTTACGGTGGACCTGTACTGGTCCGGCGAGGGTGGGTCCACCGGAGGCAGGGGGAGGAAGGTGATGCTAGTTGACGTGGCAACACGGGATTGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

58.33

Weight (kDa)

9.82

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024642)

Species Orthologous Gene IDs
pyrus_communis pycom05g22230 pycom05g22260 pycom09g06210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 185, 446
AccB1I GGYRCC 2 cut(s) 1232, 1267
AccB7I CCANNNNNTGG 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 875
AccII CGCG 3 cut(s) 720, 913, 1161
AciI CCGC 9 cut(s) 596, 718, 750, 847, 873, 947, 1008, 1072, 1289
AclWI GGATC 3 cut(s) 834, 847, 923
AcoI YGGCCR 3 cut(s) 359, 728, 1419
AcsI RAATTY 5 cut(s) 121, 272, 376, 449, 861
AcvI CACGTG 1 cut(s) 734
AfaI GTAC 5 cut(s) 45, 746, 1154, 1362, 1472
AfiI CCNNNNNNNGG 4 cut(s) 368, 1077, 1078, 1503
AflIII ACRYGT 1 cut(s) 1368
AgsI TTSAA 6 cut(s) 105, 128, 258, 278, 989, 1204
AjiI CACGTC 1 cut(s) 1531
AjnI CCWGG 3 cut(s) 853, 977, 1391
AjuI GAANNNNNNNTTGG 2 cut(s) 759, 791
AluBI AGCT 2 cut(s) 319, 761
AluI AGCT 2 cut(s) 319, 761
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 3 cut(s) 834, 847, 923
AoxI GGCC 6 cut(s) 37, 359, 728, 818, 856, 1419
ApoI RAATTY 5 cut(s) 121, 272, 376, 449, 861
AseI ATTAAT 1 cut(s) 86
AspLEI GCGC 2 cut(s) 308, 1163
AspS9I GGNCC 6 cut(s) 818, 974, 1395, 1465, 1476, 1491
AsuC2I CCSGG 1 cut(s) 1251
AsuHPI GGTGA 7 cut(s) 551, 563, 571, 620, 680, 1456, 1528
AvaII GGWCC 5 cut(s) 974, 1395, 1465, 1476, 1491
AxyI CCTNAGG 1 cut(s) 1014
BaeGI GKGCMC 2 cut(s) 515, 1272
BalI TGGCCA 1 cut(s) 361
BamHI GGATCC 1 cut(s) 839
BanI GGYRCC 2 cut(s) 1232, 1267
BauI CACGAG 2 cut(s) 33, 1178
BbrPI CACGTG 1 cut(s) 734
BccI CCATC 4 cut(s) 181, 531, 633, 699
BceAI ACGGC 1 cut(s) 1224
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 3 cut(s) 855, 979, 1393
BcnI CCSGG 1 cut(s) 1251
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 177, 1523
BfmI CTRYAG 1 cut(s) 1212
BglII AGATCT 2 cut(s) 93, 824
BisI GCNGC 2 cut(s) 750, 948
BlsI GCNGC 2 cut(s) 751, 949
Bme1390I CCNGG 4 cut(s) 855, 979, 1251, 1393
Bme18I GGWCC 5 cut(s) 974, 1395, 1465, 1476, 1491
BmgBI CACGTC 1 cut(s) 1531
BmgT120I GGNCC 6 cut(s) 818, 974, 1395, 1465, 1476, 1491
BmiI GGNNCC 5 cut(s) 841, 976, 1234, 1269, 1492
BmrFI CCNGG 4 cut(s) 855, 979, 1251, 1393
BmsI GCATC 5 cut(s) 340, 505, 1196, 1310, 1509
BpuMI CCSGG 1 cut(s) 1251
Bsa29I ATCGAT 1 cut(s) 808
BsaAI YACGTR 3 cut(s) 47, 734, 955
BsaJI CCNNGG 1 cut(s) 977
BsaWI WCCGGW 1 cut(s) 1496
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 1077, 1078, 1503
Bse1I ACTGG 1 cut(s) 1478
Bse21I CCTNAGG 1 cut(s) 1014
BseBI CCWGG 3 cut(s) 855, 979, 1393
BseCI ATCGAT 1 cut(s) 808
BseDI CCNNGG 1 cut(s) 977
BseGI GGATG 4 cut(s) 1024, 1301, 1435, 1554
BseLI CCNNNNNNNGG 4 cut(s) 368, 1077, 1078, 1503
BseMII CTCAG 2 cut(s) 1005, 1299
BseNI ACTGG 1 cut(s) 1478
BseRI GAGGAG 1 cut(s) 608
BseSI GKGCMC 2 cut(s) 515, 1272
BseX3I CGGCCG 1 cut(s) 1419
Bsh1236I CGCG 3 cut(s) 720, 913, 1161
Bsh1285I CGRYCG 2 cut(s) 808, 1422
BshFI GGCC 6 cut(s) 39, 361, 730, 820, 858, 1421
BshNI GGYRCC 2 cut(s) 1232, 1267
BshVI ATCGAT 1 cut(s) 808
BsiEI CGRYCG 2 cut(s) 808, 1422
BsiSI CCGG 3 cut(s) 1251, 1479, 1497
BslFI GGGAC 1 cut(s) 952
BslI CCNNNNNNNGG 4 cut(s) 368, 1077, 1078, 1503
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 952
BsmI GAATGC 2 cut(s) 702, 1451
BsnI GGCC 6 cut(s) 39, 361, 730, 820, 858, 1421
Bsp1286I GDGCHC 2 cut(s) 515, 1272
Bsp143I GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
BspACI CCGC 9 cut(s) 596, 718, 750, 847, 873, 947, 1008, 1072, 1289
BspANI GGCC 6 cut(s) 39, 361, 730, 820, 858, 1421
BspCNI CTCAG 2 cut(s) 1006, 1298
BspDI ATCGAT 1 cut(s) 808
BspFNI CGCG 3 cut(s) 720, 913, 1161
BspLI GGNNCC 5 cut(s) 841, 976, 1234, 1269, 1492
BspPI GGATC 3 cut(s) 834, 847, 923
BspT107I GGYRCC 2 cut(s) 1232, 1267
BsrBI CCGCTC 1 cut(s) 875
BsrI ACTGG 1 cut(s) 1478
BssECI CCNNGG 1 cut(s) 977
BssMI GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
BssSI CACGAG 2 cut(s) 33, 1178
Bst2BI CACGAG 2 cut(s) 33, 1178
Bst2UI CCWGG 3 cut(s) 855, 979, 1393
Bst4CI ACNGT 3 cut(s) 268, 586, 1462
Bst6I CTCTTC 2 cut(s) 204, 546
BstBAI YACGTR 3 cut(s) 47, 734, 955
BstC8I GCNNGC 1 cut(s) 1102
BstDEI CTNAG 3 cut(s) 97, 1014, 1285
BstF5I GGATG 4 cut(s) 1024, 1301, 1435, 1554
BstFNI CGCG 3 cut(s) 720, 913, 1161
BstHHI GCGC 2 cut(s) 308, 1163
BstKTI GATC 8 cut(s) 9, 96, 397, 808, 812, 827, 842, 931
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
BstMCI CGRYCG 2 cut(s) 808, 1422
BstMWI GCNNNNNNNGC 2 cut(s) 758, 855
BstNI CCWGG 3 cut(s) 855, 979, 1393
BstNSI RCATGY 2 cut(s) 1104, 1372
BstSCI CCNGG 4 cut(s) 853, 977, 1249, 1391
BstSFI CTRYAG 1 cut(s) 1212
BstSLI GKGCMC 2 cut(s) 515, 1272
BstSNI TACGTA 1 cut(s) 47
BstUI CGCG 3 cut(s) 720, 913, 1161
BstX2I RGATCY 4 cut(s) 93, 824, 839, 928
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 4 cut(s) 93, 824, 839, 928
BstZI CGGCCG 1 cut(s) 1419
Bsu15I ATCGAT 1 cut(s) 808
Bsu36I CCTNAGG 1 cut(s) 1014
BsuRI GGCC 6 cut(s) 39, 361, 730, 820, 858, 1421
BsuTUI ATCGAT 1 cut(s) 808
BtrI CACGTC 1 cut(s) 1531
BtsCI GGATG 4 cut(s) 1024, 1301, 1435, 1554
Cac8I GCNNGC 1 cut(s) 1102
CfoI GCGC 2 cut(s) 308, 1163
Cfr13I GGNCC 6 cut(s) 818, 974, 1395, 1465, 1476, 1491
ClaI ATCGAT 1 cut(s) 808
CseI GACGC 1 cut(s) 1057
CsiI ACCWGGT 1 cut(s) 1391
Csp6I GTAC 5 cut(s) 44, 745, 1153, 1361, 1471
CspCI CAANNNNNGTGG 2 cut(s) 1207, 1242
CviAII CATG 5 cut(s) 1101, 1164, 1354, 1369, 1435
CviQI GTAC 5 cut(s) 44, 745, 1153, 1361, 1471
DdeI CTNAG 3 cut(s) 97, 1014, 1285
DpnI GATC 8 cut(s) 8, 95, 396, 807, 811, 826, 841, 930
DpnII GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
DraI TTTAAA 3 cut(s) 249, 459, 1066
EaeI YGGCCR 3 cut(s) 359, 728, 1419
EagI CGGCCG 1 cut(s) 1419
Eam1104I CTCTTC 2 cut(s) 204, 546
EarI CTCTTC 2 cut(s) 204, 546
EclXI CGGCCG 1 cut(s) 1419
Eco105I TACGTA 1 cut(s) 47
Eco47I GGWCC 5 cut(s) 974, 1395, 1465, 1476, 1491
Eco52I CGGCCG 1 cut(s) 1419
Eco72I CACGTG 1 cut(s) 734
Eco81I CCTNAGG 1 cut(s) 1014
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 3 cut(s) 853, 977, 1391
EcoT22I ATGCAT 1 cut(s) 1189
FaeI CATG 5 cut(s) 1104, 1167, 1357, 1372, 1438
FaqI GGGAC 1 cut(s) 952
FatI CATG 5 cut(s) 1100, 1163, 1353, 1368, 1434
FauI CCCGC 4 cut(s) 854, 1015, 1079, 1282
FauNDI CATATG 2 cut(s) 78, 996
Fnu4HI GCNGC 2 cut(s) 750, 948
FokI GGATG 3 cut(s) 1031, 1288, 1442
Fsp4HI GCNGC 2 cut(s) 750, 948
FspBI CTAG 2 cut(s) 177, 1523
GlaI GCGC 2 cut(s) 307, 1162
GluI GCNGC 2 cut(s) 750, 948
HaeIII GGCC 6 cut(s) 39, 361, 730, 820, 858, 1421
HapII CCGG 3 cut(s) 1251, 1479, 1497
HgaI GACGC 1 cut(s) 1057
HhaI GCGC 2 cut(s) 308, 1163
Hin1II CATG 5 cut(s) 1104, 1167, 1357, 1372, 1438
Hin6I GCGC 2 cut(s) 306, 1161
HinP1I GCGC 2 cut(s) 306, 1161
HincII GTYRAC 4 cut(s) 1242, 1278, 1374, 1528
HindII GTYRAC 4 cut(s) 1242, 1278, 1374, 1528
HinfI GANTC 6 cut(s) 278, 969, 1091, 1106, 1415, 1424
HpaII CCGG 3 cut(s) 1251, 1479, 1497
HphI GGTGA 7 cut(s) 551, 563, 571, 620, 680, 1456, 1528
Hpy166II GTNNAC 7 cut(s) 1153, 1242, 1278, 1374, 1465, 1494, 1528
Hpy188I TCNGA 6 cut(s) 345, 375, 805, 974, 1399, 1429
Hpy188III TCNNGA 1 cut(s) 177
Hpy8I GTNNAC 7 cut(s) 1153, 1242, 1278, 1374, 1465, 1494, 1528
HpyAV CCTTC 4 cut(s) 695, 1166, 1204, 1507
HpyCH4III ACNGT 3 cut(s) 268, 586, 1462
HpyCH4IV ACGT 7 cut(s) 46, 733, 907, 954, 984, 1346, 1530
HpyCH4V TGCA 7 cut(s) 19, 76, 290, 496, 1100, 1187, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 758, 855
HpyF3I CTNAG 3 cut(s) 97, 1014, 1285
HpySE526I ACGT 7 cut(s) 46, 733, 907, 954, 984, 1346, 1530
Hsp92II CATG 5 cut(s) 1104, 1167, 1357, 1372, 1438
HspAI GCGC 2 cut(s) 306, 1161
Kzo9I GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
LmnI GCTCC 3 cut(s) 720, 880, 1320
LweI GCATC 5 cut(s) 340, 505, 1196, 1310, 1509
MabI ACCWGGT 1 cut(s) 1391
MaeI CTAG 2 cut(s) 177, 1523
MaeII ACGT 7 cut(s) 46, 733, 907, 954, 984, 1346, 1530
MalI GATC 8 cut(s) 8, 95, 396, 807, 811, 826, 841, 930
MbiI CCGCTC 1 cut(s) 875
MboI GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
MboII GAAGA 4 cut(s) 221, 533, 819, 893
MfeI CAATTG 2 cut(s) 291, 933
MflI RGATCY 4 cut(s) 93, 824, 839, 928
MhlI GDGCHC 2 cut(s) 515, 1272
MlsI TGGCCA 1 cut(s) 361
MluNI TGGCCA 1 cut(s) 361
MlyI GAGTC 3 cut(s) 1100, 1409, 1433
MmeI TCCRAC 4 cut(s) 172, 679, 803, 1407
Mox20I TGGCCA 1 cut(s) 361
Mph1103I ATGCAT 1 cut(s) 1189
MscI TGGCCA 1 cut(s) 361
MseI TTAA 8 cut(s) 86, 90, 161, 248, 270, 419, 458, 1065
MslI CAYNNNNRTG 1 cut(s) 1182
Msp20I TGGCCA 1 cut(s) 361
MspA1I CMGCKG 1 cut(s) 1289
MspI CCGG 3 cut(s) 1251, 1479, 1497
MspR9I CCNGG 4 cut(s) 855, 979, 1251, 1393
MunI CAATTG 2 cut(s) 291, 933
Mva1269I GAATGC 2 cut(s) 702, 1451
MvaI CCWGG 3 cut(s) 855, 979, 1393
MvnI CGCG 3 cut(s) 720, 913, 1161
MwoI GCNNNNNNNGC 2 cut(s) 758, 855
NciI CCSGG 1 cut(s) 1251
NdeI CATATG 2 cut(s) 78, 996
NdeII GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
NlaIII CATG 5 cut(s) 1104, 1167, 1357, 1372, 1438
NlaIV GGNNCC 5 cut(s) 841, 976, 1234, 1269, 1492
NmeAIII GCCGAG 2 cut(s) 1397, 1447
NsiI ATGCAT 1 cut(s) 1189
NspI RCATGY 2 cut(s) 1104, 1372
PacI TTAATTAA 1 cut(s) 90
PaeI GCATGC 1 cut(s) 1104
PciI ACATGT 1 cut(s) 1368
PcsI WCGNNNNNNNCGW 1 cut(s) 1343
PctI GAATGC 2 cut(s) 702, 1451
PfeI GAWTC 3 cut(s) 278, 969, 1106
PflMI CCANNNNNTGG 1 cut(s) 368
PkrI GCNGC 2 cut(s) 751, 949
Ple19I CGATCG 1 cut(s) 808
PleI GAGTC 3 cut(s) 1099, 1409, 1432
PmaCI CACGTG 1 cut(s) 734
PmlI CACGTG 1 cut(s) 734
PpsI GAGTC 3 cut(s) 1099, 1409, 1432
Ppu21I YACGTR 3 cut(s) 47, 734, 955
PscI ACATGT 1 cut(s) 1368
PshBI ATTAAT 1 cut(s) 86
PsiI TTATAA 2 cut(s) 185, 446
Psp6I CCWGG 3 cut(s) 853, 977, 1391
PspCI CACGTG 1 cut(s) 734
PspGI CCWGG 3 cut(s) 853, 977, 1391
PspN4I GGNNCC 5 cut(s) 841, 976, 1234, 1269, 1492
PspPI GGNCC 6 cut(s) 818, 974, 1395, 1465, 1476, 1491
PsuI RGATCY 4 cut(s) 93, 824, 839, 928
PvuI CGATCG 1 cut(s) 808
RsaI GTAC 5 cut(s) 45, 746, 1154, 1362, 1472
RsaNI GTAC 5 cut(s) 44, 745, 1153, 1361, 1471
RseI CAYNNNNRTG 1 cut(s) 1182
SaqAI TTAA 8 cut(s) 86, 90, 161, 248, 270, 419, 458, 1065
SatI GCNGC 2 cut(s) 750, 948
Sau3AI GATC 8 cut(s) 6, 93, 394, 805, 809, 824, 839, 928
Sau96I GGNCC 6 cut(s) 818, 974, 1395, 1465, 1476, 1491
SchI GAGTC 3 cut(s) 1100, 1409, 1433
ScrFI CCNGG 4 cut(s) 855, 979, 1251, 1393
SduI GDGCHC 2 cut(s) 515, 1272
SexAI ACCWGGT 1 cut(s) 1391
SfaNI GCATC 5 cut(s) 340, 505, 1196, 1310, 1509
SfcI CTRYAG 1 cut(s) 1212
SinI GGWCC 5 cut(s) 974, 1395, 1465, 1476, 1491
SmiMI CAYNNNNRTG 1 cut(s) 1182
SnaBI TACGTA 1 cut(s) 47
SphI GCATGC 1 cut(s) 1104
SsiI CCGC 9 cut(s) 596, 718, 750, 847, 873, 947, 1008, 1072, 1289
SspMI CTAG 2 cut(s) 177, 1523
StyD4I CCNGG 4 cut(s) 853, 977, 1249, 1391
TaaI ACNGT 3 cut(s) 268, 586, 1462
TaiI ACGT 7 cut(s) 49, 736, 910, 957, 987, 1349, 1533
TaqI TCGA 4 cut(s) 9, 772, 808, 1034
TatI WGTACW 2 cut(s) 1360, 1470
TauI GCSGC 2 cut(s) 752, 950
TfiI GAWTC 3 cut(s) 278, 969, 1106
Tru1I TTAA 8 cut(s) 86, 90, 161, 248, 270, 419, 458, 1065
Tru9I TTAA 8 cut(s) 86, 90, 161, 248, 270, 419, 458, 1065
TspDTI ATGAA 5 cut(s) 179, 578, 695, 810, 1044
Van91I CCANNNNNTGG 1 cut(s) 368
VpaK11BI GGWCC 5 cut(s) 974, 1395, 1465, 1476, 1491
VspI ATTAAT 1 cut(s) 86
XapI RAATTY 5 cut(s) 121, 272, 376, 449, 861
XbaI TCTAGA 1 cut(s) 176
XceI RCATGY 2 cut(s) 1104, 1372
XcmI CCANNNNNNNNNTGG 2 cut(s) 885, 939
XspI CTAG 2 cut(s) 177, 1523
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.