pycom05g31470

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029289
Physical Location & Seq
Reverse (-)
31199544 .. 31200333
790 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31470.2

Sequence Viewer

Length: 480 bp
ATGTCCGTTGTCCTTGAGAAGTTTGCTGACATGGGCATTGACACCCCCGGTGTGGTCGCCCTTCTAGGAGCTCACAGCGTTGGAAGAACACACTGTGTGAAGCTGGTGCACCGGTTGTACCCAGAGGTTGACCCTCAGCTCAACCCTGATCATGTCCCTCACATGCTCAAGAAGTGCCCTGATGCTATCCCCGACCCCAAAGCCGTTCAATACGTGAGAAATGACCGTGGTACCCCCATGATCTTCGATAATAACTACTACAGAAACATCTTGGACAACAAGGGTTTGATGATGGTGGACCACCAGCTAGCCACGGACAAGAGGACAAAACCCTACGTCAAAAAAATGGCCAAGAGCCAAGACTACTTCTTCAAGGAGTTTTCGAGAGCCTTCACCCTTCTCTCCGAGAACAACCCTCTCACTGGAAACAAGGGCGAGATCCGACAGCAGTGCAATGTTGCCAACAAGATCCGCGACTAG

Protein Analysis

160

Amino Acids

18.14

Weight (kDa)

9.11

Isoelectric Point (pI)

24.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029289)

Species Orthologous Gene IDs
pyrus_communis pycom05g31470
rosa_samantha Rh5DG025700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 230
AccII CGCG 1 cut(s) 474
AciI CCGC 1 cut(s) 472
AclWI GGATC 2 cut(s) 433, 463
AcoI YGGCCR 1 cut(s) 348
AdeI CACNNNGTG 2 cut(s) 95, 97
AfaI GTAC 2 cut(s) 119, 232
AfiI CCNNNNNNNGG 2 cut(s) 52, 422
AgeI ACCGGT 1 cut(s) 111
AgsI TTSAA 2 cut(s) 209, 373
AluBI AGCT 4 cut(s) 71, 103, 139, 307
AluI AGCT 4 cut(s) 71, 103, 139, 307
Alw21I GWGCWC 2 cut(s) 73, 111
Alw44I GTGCAC 1 cut(s) 107
AlwI GGATC 2 cut(s) 433, 463
AoxI GGCC 1 cut(s) 348
ApaLI GTGCAC 1 cut(s) 107
AsiGI ACCGGT 1 cut(s) 111
Asp718I GGTACC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 385
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 2 cut(s) 111, 179
BaeI ACNNNNGTAYC 2 cut(s) 214, 247
BalI TGGCCA 1 cut(s) 350
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 2 cut(s) 73, 111
BbvCI CCTCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 286
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 1 cut(s) 48
BfaI CTAG 3 cut(s) 65, 308, 478
BfmI CTRYAG 1 cut(s) 259
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 172
BmtI GCTAGC 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 35, 152
BpuMI CCSGG 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 46, 226, 312
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 422
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 427
Bse3DI GCAATG 1 cut(s) 460
BseDI CCNNGG 3 cut(s) 46, 226, 312
BseLI CCNNNNNNNGG 2 cut(s) 52, 422
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 427
BseSI GKGCMC 2 cut(s) 111, 179
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 1 cut(s) 350
BshNI GGYRCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 73, 111
BsiSI CCGG 2 cut(s) 48, 112
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 52, 422
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 1 cut(s) 350
Bsp1286I GDGCHC 3 cut(s) 73, 111, 179
Bsp143I GATC 4 cut(s) 148, 240, 438, 468
BspACI CCGC 1 cut(s) 472
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 1 cut(s) 148
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 1 cut(s) 232
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 2 cut(s) 433, 463
BspT107I GGYRCC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 3 cut(s) 46, 226, 312
BssMI GATC 4 cut(s) 148, 240, 438, 468
Bst4CI ACNGT 2 cut(s) 95, 227
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 2 cut(s) 226, 312
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 4 cut(s) 151, 243, 441, 471
BstMBI GATC 4 cut(s) 148, 240, 438, 468
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 2 cut(s) 111, 179
BstUI CGCG 1 cut(s) 474
BstX2I RGATCY 2 cut(s) 438, 468
BstYI RGATCY 2 cut(s) 438, 468
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 2 cut(s) 226, 312
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 3 cut(s) 91, 420, 455
Cac8I GCNNGC 1 cut(s) 309
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 118, 231
CspAI ACCGGT 1 cut(s) 111
CviAII CATG 4 cut(s) 31, 152, 163, 238
CviJI RGCY 9 cut(s) 71, 103, 139, 203, 307, 311, 350, 357, 389
CviKI_1 RGCY 9 cut(s) 71, 103, 139, 203, 307, 311, 350, 357, 389
CviQI GTAC 2 cut(s) 118, 231
DdeI CTNAG 1 cut(s) 135
DpnI GATC 4 cut(s) 150, 242, 440, 470
DpnII GATC 4 cut(s) 148, 240, 438, 468
DraIII CACNNNGTG 2 cut(s) 95, 97
EaeI YGGCCR 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 4 cut(s) 34, 155, 166, 241
FaiI YATR 4 cut(s) 32, 153, 164, 239
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 30, 151, 162, 237
FbaI TGATCA 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 3 cut(s) 65, 308, 478
HaeIII GGCC 1 cut(s) 350
HapII CCGG 2 cut(s) 48, 112
Hin1II CATG 4 cut(s) 34, 155, 166, 241
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HpaII CCGG 2 cut(s) 48, 112
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 3 cut(s) 109, 130, 298
Hpy188I TCNGA 2 cut(s) 406, 443
Hpy188III TCNNGA 2 cut(s) 169, 384
Hpy8I GTNNAC 3 cut(s) 109, 130, 298
HpyAV CCTTC 3 cut(s) 71, 400, 407
HpyCH4III ACNGT 2 cut(s) 95, 227
HpyCH4IV ACGT 2 cut(s) 213, 336
HpyCH4V TGCA 2 cut(s) 109, 453
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 2 cut(s) 213, 336
Hsp92II CATG 4 cut(s) 34, 155, 166, 241
KpnI GGTACC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 4 cut(s) 148, 240, 438, 468
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 8 cut(s) 61, 89, 125, 135, 159, 192, 317, 408
LweI GCATC 1 cut(s) 172
MaeI CTAG 3 cut(s) 65, 308, 478
MaeII ACGT 2 cut(s) 213, 336
MalI GATC 4 cut(s) 150, 242, 440, 470
MboI GATC 4 cut(s) 148, 240, 438, 468
MboII GAAGA 3 cut(s) 96, 235, 361
MflI RGATCY 2 cut(s) 438, 468
MhlI GDGCHC 3 cut(s) 73, 111, 179
MlsI TGGCCA 1 cut(s) 350
MluNI TGGCCA 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 61, 466
MnlI CCTC 5 cut(s) 118, 144, 168, 315, 426
Mox20I TGGCCA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 350
MspI CCGG 2 cut(s) 48, 112
MspR9I CCNGG 1 cut(s) 48
MvnI CGCG 1 cut(s) 474
NciI CCSGG 1 cut(s) 48
NdeII GATC 4 cut(s) 148, 240, 438, 468
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 4 cut(s) 34, 155, 166, 241
NlaIV GGNNCC 1 cut(s) 232
NspI RCATGY 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 111
Ppu21I YACGTR 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 2 cut(s) 438, 468
RsaI GTAC 2 cut(s) 119, 232
RsaNI GTAC 2 cut(s) 118, 231
SacI GAGCTC 1 cut(s) 73
Sau3AI GATC 4 cut(s) 148, 240, 438, 468
Sau96I GGNCC 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 3 cut(s) 73, 111, 179
SetI ASST 7 cut(s) 73, 105, 129, 141, 216, 309, 339
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 2 cut(s) 14, 167
SmoI CTYRAG 2 cut(s) 14, 167
SsiI CCGC 1 cut(s) 472
SspMI CTAG 3 cut(s) 65, 308, 478
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 2 cut(s) 95, 227
TaiI ACGT 2 cut(s) 216, 339
TaqI TCGA 2 cut(s) 246, 383
TscAI CASTG 3 cut(s) 98, 427, 455
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 3 cut(s) 98, 427, 455
VneI GTGCAC 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 298
XceI RCATGY 1 cut(s) 166
XspI CTAG 3 cut(s) 65, 308, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.