Rh5DG025700

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0029289
Physical Location & Seq
Forward (+)
1786893 .. 1787207
315 bp
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UTR
Exon/CDS
Intron
Rh5DG025700.1

Sequence Viewer

Length: 315 bp
ATGCTCAAGAAGTGCCCTGATGCAATCCCTGACCCCAAGGCAGTCCAGTACGTGAGAAATGACCGTGGTACCCCCATGATCTTCGACAACAACTACTACAGGAACATCTTGGACAACAAGGGTTTGATGATGGTGGATCACCAGCTTGCTACAGACAAGAGGACCAAGCCCTATGTCAAGAAAATGGCCAAAAGCCAAGACTACTTCTTCAAGGAGTTCACAAGAGCCTTCACCATTCTCTCCGAGAACAACCCTCTCACCGGAGACAAGGGGGAGATCAGGCAACAGTGCAATGTGGCCAACAAGCTCCACTAG

Protein Analysis

104

Amino Acids

12.14

Weight (kDa)

9.42

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 4 - 65 8.9e-12 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029289)

Species Orthologous Gene IDs
pyrus_communis pycom05g31470
rosa_samantha Rh5DG025700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 68
AclWI GGATC 1 cut(s) 144
AcoI YGGCCR 2 cut(s) 186, 297
AfaI GTAC 2 cut(s) 50, 70
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 1 cut(s) 211
AluBI AGCT 2 cut(s) 145, 307
AluI AGCT 2 cut(s) 145, 307
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 1 cut(s) 144
AoxI GGCC 2 cut(s) 186, 297
Asp718I GGTACC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 131, 223, 250
AvaII GGWCC 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 17
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BalI TGGCCA 2 cut(s) 188, 299
BanI GGYRCC 1 cut(s) 68
BccI CCATC 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 1 cut(s) 313
BfmI CTRYAG 2 cut(s) 97, 150
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 70
BmsI GCATC 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 36, 64
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 36, 64
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 46
BseSI GKGCMC 1 cut(s) 17
BshFI GGCC 2 cut(s) 188, 299
BshNI GGYRCC 1 cut(s) 68
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 258
BsnI GGCC 2 cut(s) 188, 299
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 78, 136, 276
BspANI GGCC 2 cut(s) 188, 299
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 36, 64
BssMI GATC 3 cut(s) 78, 136, 276
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 65, 288
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 64
BstKTI GATC 3 cut(s) 81, 139, 279
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 3 cut(s) 78, 136, 276
BstSFI CTRYAG 2 cut(s) 97, 150
BstSLI GKGCMC 1 cut(s) 17
BsuRI GGCC 2 cut(s) 188, 299
BtgI CCRYGG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 147
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 49, 69
CviAII CATG 1 cut(s) 76
CviJI RGCY 7 cut(s) 145, 169, 188, 195, 227, 299, 307
CviKI_1 RGCY 7 cut(s) 145, 169, 188, 195, 227, 299, 307
CviQI GTAC 2 cut(s) 49, 69
DpnI GATC 3 cut(s) 80, 138, 278
DpnII GATC 3 cut(s) 78, 136, 276
EaeI YGGCCR 2 cut(s) 186, 297
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 1 cut(s) 79
FaiI YATR 2 cut(s) 77, 174
FatI CATG 1 cut(s) 75
FspBI CTAG 1 cut(s) 313
HaeIII GGCC 2 cut(s) 188, 299
HapII CCGG 1 cut(s) 261
Hin1II CATG 1 cut(s) 79
HpaII CCGG 1 cut(s) 261
HphI GGTGA 3 cut(s) 131, 223, 250
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 2 cut(s) 7, 178
Hpy8I GTNNAC 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 65, 288
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 23, 291
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 79
KpnI GGTACC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 78, 136, 276
LmnI GCTCC 1 cut(s) 312
LpnPI CCDG 7 cut(s) 30, 42, 59, 85, 155, 265, 274
LweI GCATC 1 cut(s) 10
MaeI CTAG 1 cut(s) 313
MaeII ACGT 1 cut(s) 51
MalI GATC 3 cut(s) 80, 138, 278
MboI GATC 3 cut(s) 78, 136, 276
MboII GAAGA 2 cut(s) 73, 199
MhlI GDGCHC 1 cut(s) 17
MlsI TGGCCA 2 cut(s) 188, 299
MluNI TGGCCA 2 cut(s) 188, 299
MnlI CCTC 2 cut(s) 153, 264
Mox20I TGGCCA 2 cut(s) 188, 299
MscI TGGCCA 2 cut(s) 188, 299
Msp20I TGGCCA 2 cut(s) 188, 299
MspI CCGG 1 cut(s) 261
NdeII GATC 3 cut(s) 78, 136, 276
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 70
Ppu21I YACGTR 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 162
RsaI GTAC 2 cut(s) 50, 70
RsaNI GTAC 2 cut(s) 49, 69
Sau3AI GATC 3 cut(s) 78, 136, 276
Sau96I GGNCC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 17
SetI ASST 3 cut(s) 54, 147, 309
SfaNI GCATC 1 cut(s) 10
SfcI CTRYAG 2 cut(s) 97, 150
SinI GGWCC 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
SspMI CTAG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 65, 288
TaiI ACGT 1 cut(s) 54
TaqI TCGA 1 cut(s) 84
TscAI CASTG 1 cut(s) 293
TspRI CASTG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 162
XspI CTAG 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.