pycom05g32670

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0028752
Physical Location & Seq
Reverse (-)
31981976 .. 31982866
891 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32670.2

Sequence Viewer

Length: 807 bp
ATGATTTCAATGTTTGGGCTTACTACTGATAAACATATTGCTTTACTTTATTCATTCCGGGAATCCTGGGCTCAATCCTATATTAAGGGTTACTTTCTTGCTTGTATGGCAACGACAATATATTCAAAGTCTGTAGATGCATTTTTCAAAGGAATTTTCAGTGCACAAACATGTTTGCGTAGCTTTTTCGAGCAGGTTGGTATTTCTGCCAATTTTCAAAATCAGTCACATCGAGAGATGCAGTATATGCATATTAAAACAAGCATTCCTATTGAAGAGCACGCAAGGAGTATTTTGACACCTTTTGCCTTCAATGCTTTCCAGCATGAACTGGTGCTCGCCATGCAATATGCTGTATCTGAAATGGCCAATGGGTCATATCTTGTGCAGCATTTTAAGAAGATTGAGGGAGAGCGTCTTGTGATATGGATACCAGAAGAGGAACAGGTACACTGTTCCTGTAAAGAGTTTGAATCTTCAGGATTGTTATGCAGACATTCTCTACGTGTGTATGTGGTCAAGAACTACTTTCAGCTTCCTGACAAATACTGTTTAAATAGATGGCGGAAAGAAGGCTCGCTTGATTATTATGATGACCACATTGCTCGGACTAGTGATGAGGAATGGTTTCAAGATTATCAATGCCTTACTGATACCCTGTTAACAGAATCATCAGTTACAAAGGAGCGTTCTGATTATATTCACAGGGAATTGACAACACAGCTCACAAGGATTCTTAATGAGGTTAGAAATATGCCAGAATGTGAAGGAGTAGCTATGGATCTGACATTTTCCCCTACTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

269

Amino Acids

31.32

Weight (kDa)

5.7

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028752)

Species Orthologous Gene IDs
malus_domestica MD00G1057100.v1.1
pyrus_communis pycom05g32670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 184
AciI CCGC 1 cut(s) 565
AclWI GGATC 1 cut(s) 789
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 1 cut(s) 450
AflIII ACRYGT 2 cut(s) 170, 505
AgsI TTSAA 8 cut(s) 9, 126, 148, 218, 275, 313, 473, 632
AhlI ACTAGT 1 cut(s) 611
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 4 cut(s) 183, 535, 724, 776
AluI AGCT 4 cut(s) 183, 535, 724, 776
Alw21I GWGCWC 3 cut(s) 166, 282, 339
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 1 cut(s) 789
AoxI GGCC 1 cut(s) 366
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 388
ApoI RAATTY 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 627
AsuC2I CCSGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 166
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 3 cut(s) 166, 282, 339
BbvI GCAGC 1 cut(s) 400
BccI CCATC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 67
BciVI GTATCC 1 cut(s) 423
BcnI CCSGG 1 cut(s) 59
BcuI ACTAGT 1 cut(s) 611
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 184
BfuI GTATCC 1 cut(s) 423
BisI GCNGC 1 cut(s) 389
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 2 cut(s) 59, 67
BmrFI CCNGG 2 cut(s) 59, 67
BmsI GCATC 2 cut(s) 127, 228
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 66
Bse1I ACTGG 2 cut(s) 336, 805
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 600
BseNI ACTGG 2 cut(s) 336, 805
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 1 cut(s) 400
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 368
BsiHKAI GWGCWC 3 cut(s) 166, 282, 339
BsiSI CCGG 1 cut(s) 58
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 1 cut(s) 368
Bsp1286I GDGCHC 4 cut(s) 73, 166, 282, 339
Bsp143I GATC 1 cut(s) 781
BspACI CCGC 1 cut(s) 565
BspANI GGCC 1 cut(s) 368
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 1 cut(s) 789
BspQI GCTCTTC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 600
BsrI ACTGG 2 cut(s) 336, 805
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 781
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 2 cut(s) 455, 551
Bst6I CTCTTC 2 cut(s) 270, 432
BstAPI GCANNNNNTGC 1 cut(s) 247
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 282, 339, 578
BstKTI GATC 1 cut(s) 784
BstMBI GATC 1 cut(s) 781
BstMWI GCNNNNNNNGC 4 cut(s) 107, 247, 314, 343
BstNI CCWGG 1 cut(s) 67
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 2 cut(s) 57, 65
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 1 cut(s) 368
BtsIMutI CAGTG 2 cut(s) 166, 451
BveI ACCTGC 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 282, 339, 578
CseI GACGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 449
CviAII CATG 3 cut(s) 171, 326, 343
CviJI RGCY 9 cut(s) 19, 71, 183, 368, 535, 576, 724, 776, 804
CviKI_1 RGCY 9 cut(s) 19, 71, 183, 368, 535, 576, 724, 776, 804
CviQI GTAC 1 cut(s) 449
DpnI GATC 1 cut(s) 783
DpnII GATC 1 cut(s) 781
DraI TTTAAA 1 cut(s) 555
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 270, 432
EarI CTCTTC 2 cut(s) 270, 432
EciI GGCGGA 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 65
EcoT22I ATGCAT 2 cut(s) 142, 252
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 3 cut(s) 174, 329, 346
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 512, 544, 564, 596
FatI CATG 3 cut(s) 170, 325, 342
Fnu4HI GCNGC 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 389
FspBI CTAG 1 cut(s) 612
GluI GCNGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 368
HapII CCGG 1 cut(s) 58
HgaI GACGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 174, 329, 346
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 4 cut(s) 62, 473, 668, 733
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 1 cut(s) 58
Hpy166II GTNNAC 3 cut(s) 164, 451, 663
Hpy188I TCNGA 4 cut(s) 361, 609, 694, 786
Hpy188III TCNNGA 5 cut(s) 233, 480, 520, 539, 632
Hpy8I GTNNAC 3 cut(s) 164, 451, 663
HpyAV CCTTC 3 cut(s) 319, 566, 761
HpyCH4III ACNGT 2 cut(s) 455, 551
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 7 cut(s) 140, 164, 241, 250, 346, 388, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 247, 314, 343
HpySE526I ACGT 1 cut(s) 505
Hsp92II CATG 3 cut(s) 174, 329, 346
KspAI GTTAAC 1 cut(s) 663
Kzo9I GATC 1 cut(s) 781
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 2 cut(s) 127, 228
MaeI CTAG 1 cut(s) 612
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 3 cut(s) 89, 225, 676
MalI GATC 1 cut(s) 783
MboI GATC 1 cut(s) 781
MboII GAAGA 4 cut(s) 287, 412, 449, 468
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 4 cut(s) 73, 166, 282, 339
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 3 cut(s) 153, 211, 710
MluNI TGGCCA 1 cut(s) 368
MnlI CCTC 4 cut(s) 400, 433, 613, 736
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 2 cut(s) 142, 252
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 1 cut(s) 368
MseI TTAA 6 cut(s) 84, 255, 396, 554, 662, 738
MslI CAYNNNNRTG 1 cut(s) 169
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 59, 67
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 67
MwoI GCNNNNNNNGC 4 cut(s) 107, 247, 314, 343
NciI CCSGG 1 cut(s) 59
NdeII GATC 1 cut(s) 781
NlaIII CATG 3 cut(s) 174, 329, 346
NmuCI GTSAC 1 cut(s) 225
NsiI ATGCAT 2 cut(s) 142, 252
NspI RCATGY 1 cut(s) 174
PciI ACATGT 1 cut(s) 170
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 4 cut(s) 62, 473, 668, 733
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 1 cut(s) 390
Ppu21I YACGTR 1 cut(s) 506
PscI ACATGT 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 1 cut(s) 450
RsaNI GTAC 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 6 cut(s) 84, 255, 396, 554, 662, 738
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 1 cut(s) 781
ScrFI CCNGG 2 cut(s) 59, 67
SduI GDGCHC 4 cut(s) 73, 166, 282, 339
SetI ASST 9 cut(s) 185, 198, 304, 450, 508, 537, 726, 747, 778
SfaNI GCATC 2 cut(s) 127, 228
SfcI CTRYAG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 169
SpeI ACTAGT 1 cut(s) 611
Sse9I AATT 3 cut(s) 153, 211, 710
SsiI CCGC 1 cut(s) 565
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 2 cut(s) 57, 65
TaaI ACNGT 2 cut(s) 455, 551
TaiI ACGT 1 cut(s) 508
TaqI TCGA 2 cut(s) 189, 232
TasI AATT 3 cut(s) 153, 211, 710
TfiI GAWTC 4 cut(s) 62, 473, 668, 733
Tru1I TTAA 6 cut(s) 84, 255, 396, 554, 662, 738
Tru9I TTAA 6 cut(s) 84, 255, 396, 554, 662, 738
TscAI CASTG 2 cut(s) 166, 458
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 2 cut(s) 42, 342
TspRI CASTG 2 cut(s) 166, 458
VneI GTGCAC 1 cut(s) 162
XapI RAATTY 1 cut(s) 153
XceI RCATGY 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 1 cut(s) 612
Zsp2I ATGCAT 2 cut(s) 142, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.