pycom06g02290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0024644
Physical Location & Seq
Reverse (-)
2758888 .. 2760417
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g02290.3

Sequence Viewer

Length: 423 bp
ATGGTCCCTGTGTCAAATTTTAAACGAGAGTATCACGATTCGTCTTACTCCTCCCTCTTCTGCATCTCTCTCTCTCTCTCTCTCTCCTCGTCTCCCAATTTGCTCAGAGAAAATGGACGCCATCGACTCAGTGGTGGATCCGCTCCGAGAGTTCTCCAAGGACAGCATTCGCCTCGTCAAGCGCTGCCACAAGCCCGATCGCAAAGAATTCTCCAAGGTCGCGCTCCGGACAGCGATCGGGTTCGTCGTGATGGGTTTCGTCGGGTTCTTCGTGAAGCTGATCTTCATCCCCATCAACAACATCATCGTCGGATCTGTTTAGGTCATGCTGAAAATCAAGTTAGACGGATGATAGGTGCAGCAAACCTTAAGAAAGCATTAGCTTTTAGTTCTGATATGCTCCTTGTTTCTGTAAGACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.84

Weight (kDa)

11.54

Isoelectric Point (pI)

66.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024644)

Species Orthologous Gene IDs
pyrus_communis pycom06g02290
rosa_roxburghii Rroxscaffold_2G00077160
rosa_rugosa Rorug02G0587200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 143
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 1 cut(s) 141
AclWI GGATC 3 cut(s) 132, 145, 320
AcsI RAATTY 2 cut(s) 16, 207
AcyI GRCGYC 1 cut(s) 118
AfeI AGCGCT 1 cut(s) 183
AflII CTTAAG 1 cut(s) 368
AluBI AGCT 2 cut(s) 278, 383
AluI AGCT 2 cut(s) 278, 383
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 3 cut(s) 132, 145, 320
Aor13HI TCCGGA 1 cut(s) 226
Aor51HI AGCGCT 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 184, 359
ApoI RAATTY 2 cut(s) 16, 207
AspLEI GCGC 2 cut(s) 184, 224
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 14, 47
BamHI GGATCC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 171, 371
BccI CCATC 3 cut(s) 129, 245, 300
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcoDI GTCTC 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 185
BfrI CTTAAG 1 cut(s) 368
BisI GCNGC 2 cut(s) 185, 360
BlsI GCNGC 2 cut(s) 186, 361
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 139
BmsI GCATC 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 157, 214
BsaWI WCCGGW 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 157, 214
BseGI GGATG 2 cut(s) 286, 354
BseJI GATNNNNATC 1 cut(s) 285
BseMII CTCAG 2 cut(s) 118, 142
BseRI GAGGAG 2 cut(s) 40, 76
BseXI GCAGC 2 cut(s) 171, 371
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 2 cut(s) 200, 238
BsiEI CGRYCG 2 cut(s) 200, 238
BsiSI CCGG 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsmI GAATGC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 5 cut(s) 137, 197, 235, 280, 312
BspACI CCGC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 117, 141
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 6, 139
BspPI GGATC 3 cut(s) 132, 145, 320
BspTI CTTAAG 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 157, 214
BssMI GATC 5 cut(s) 137, 197, 235, 280, 312
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 157, 214
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 118
BstAFI CTTAAG 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 104, 128
BstF5I GGATG 2 cut(s) 286, 354
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 184, 224
BstKTI GATC 5 cut(s) 140, 200, 238, 283, 315
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 5 cut(s) 137, 197, 235, 280, 312
BstMCI CGRYCG 2 cut(s) 200, 238
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 171, 371
BstX2I RGATCY 2 cut(s) 137, 312
BstYI RGATCY 2 cut(s) 137, 312
BtsCI GGATG 2 cut(s) 286, 354
BtsIMutI CAGTG 1 cut(s) 136
CfoI GCGC 2 cut(s) 184, 224
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 126
CviAII CATG 2 cut(s) 326, 420
CviJI RGCY 3 cut(s) 194, 278, 383
CviKI_1 RGCY 3 cut(s) 194, 278, 383
DdeI CTNAG 2 cut(s) 104, 128
DpnI GATC 5 cut(s) 139, 199, 237, 282, 314
DpnII GATC 5 cut(s) 137, 197, 235, 280, 312
DraI TTTAAA 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 157, 214
Eco47I GGWCC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 207
EcoT14I CCWWGG 2 cut(s) 157, 214
ErhI CCWWGG 2 cut(s) 157, 214
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 2 cut(s) 329, 423
FaiI YATR 3 cut(s) 327, 398, 421
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FatI CATG 2 cut(s) 325, 419
Fnu4HI GCNGC 2 cut(s) 185, 360
FokI GGATG 2 cut(s) 273, 361
Fsp4HI GCNGC 2 cut(s) 185, 360
GlaI GCGC 2 cut(s) 183, 223
GluI GCNGC 2 cut(s) 185, 360
HaeII RGCGCY 1 cut(s) 185
HapII CCGG 1 cut(s) 227
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 184, 224
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 2 cut(s) 329, 423
Hin6I GCGC 2 cut(s) 182, 222
HinP1I GCGC 2 cut(s) 182, 222
HinfI GANTC 2 cut(s) 38, 126
HpaII CCGG 1 cut(s) 227
Hpy188I TCNGA 4 cut(s) 107, 147, 312, 394
Hpy188III TCNNGA 4 cut(s) 35, 227, 248, 272
Hpy99I CGWCG 3 cut(s) 249, 264, 312
HpyCH4V TGCA 2 cut(s) 63, 359
HpyF3I CTNAG 2 cut(s) 104, 128
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 2 cut(s) 329, 423
HspAI GCGC 2 cut(s) 182, 222
Kpn2I TCCGGA 1 cut(s) 226
Kzo9I GATC 5 cut(s) 137, 197, 235, 280, 312
LmnI GCTCC 3 cut(s) 148, 229, 405
LpnPI CCDG 2 cut(s) 21, 240
Lsp1109I GCAGC 2 cut(s) 171, 371
LweI GCATC 1 cut(s) 72
MalI GATC 5 cut(s) 139, 199, 237, 282, 314
MbiI CCGCTC 1 cut(s) 143
MboI GATC 5 cut(s) 137, 197, 235, 280, 312
MboII GAAGA 3 cut(s) 49, 260, 275
MflI RGATCY 2 cut(s) 137, 312
MluCI AATT 3 cut(s) 16, 97, 207
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 4 cut(s) 61, 65, 97, 183
MroI TCCGGA 1 cut(s) 226
MseI TTAA 2 cut(s) 21, 369
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 166
MvnI CGCG 1 cut(s) 222
NdeII GATC 5 cut(s) 137, 197, 235, 280, 312
NlaIII CATG 2 cut(s) 329, 423
NlaIV GGNNCC 2 cut(s) 6, 139
PcsI WCGNNNNNNNCGW 2 cut(s) 244, 268
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 2 cut(s) 186, 361
Ple19I CGATCG 2 cut(s) 200, 238
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 6, 139
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 2 cut(s) 137, 312
PvuI CGATCG 2 cut(s) 200, 238
SaqAI TTAA 2 cut(s) 21, 369
SatI GCNGC 2 cut(s) 185, 360
Sau3AI GATC 5 cut(s) 137, 197, 235, 280, 312
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 120
SetI ASST 6 cut(s) 220, 280, 325, 358, 369, 385
SfaNI GCATC 1 cut(s) 72
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 3 cut(s) 16, 97, 207
SsiI CCGC 1 cut(s) 141
StyI CCWWGG 2 cut(s) 157, 214
TaqI TCGA 1 cut(s) 124
TasI AATT 3 cut(s) 16, 97, 207
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 21, 369
Tru9I TTAA 2 cut(s) 21, 369
TscAI CASTG 1 cut(s) 136
TseI GCWGC 2 cut(s) 184, 359
TspDTI ATGAA 1 cut(s) 275
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 16, 207
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.