pycom06g03040

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0024645
Physical Location & Seq
Reverse (-)
3823067 .. 3823916
850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGTCCTTGCTTGCCGGTTCCCTTTTTGGAGTCTTCAAAGGTGTAGCTTTGGTGGTGTTTAACGCCACTGCTGGTGCATCTTCTTGTTATTTCTTGTCAAAGCTGATTGGGAGGCCCATTGTCATCTCTCTTTGGCCTGACAAGCTGCAATTTTTCCAAAACCAGGTAGCTAAAAGAAGGCAGGGACTGTTGAACTACATGTTGTTTCTCCGATTAACTCCGACCTTGCCTAATACATTTATTAATCTTGCTTCACCAATAGTTGATGTGCCTTACCATATTTTCTTACTGGCAACTTCAATTGGTCTGATACCAGCTGCGTACGTCACTGTCAGGGCCGGATTAGCTCTTGGAGAGTTACGATCCCTTGGCGATCTCTATGACTTCAACTCGATAGCCACCTTGTTCTTCATTGGAGTTGTCTCAATTACTCCCACGCTAGTGAGCAAGAACAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

154

Amino Acids

16.61

Weight (kDa)

9.93

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 2 - 114 1e-14 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024645)

Species Orthologous Gene IDs
pyrus_communis pycom06g03040
rosa_multiflora Rmu_sc0019437.1_g000001
rosa_wichuraiana Rw7G021540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 357
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 4 cut(s) 37, 193, 300, 388
AjnI CCWGG 1 cut(s) 162
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 6 cut(s) 47, 103, 145, 170, 317, 347
AluI AGCT 6 cut(s) 47, 103, 145, 170, 317, 347
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 3 cut(s) 113, 134, 336
ApeKI GCWGC 2 cut(s) 145, 317
AseI ATTAAT 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 114, 336
AsuHPI GGTGA 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 2 cut(s) 132, 304
BciT130I CCWGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 1 cut(s) 440
BisI GCNGC 2 cut(s) 146, 318
BlsI GCNGC 2 cut(s) 147, 319
Bme1390I CCNGG 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 114, 336
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 163
BseNI ACTGG 1 cut(s) 294
BseXI GCAGC 2 cut(s) 132, 304
BshFI GGCC 3 cut(s) 115, 136, 338
BsiSI CCGG 2 cut(s) 15, 339
BsiWI CGTACG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 3 cut(s) 115, 136, 338
Bsp143I GATC 2 cut(s) 362, 373
BspANI GGCC 3 cut(s) 115, 136, 338
BspLI GGNNCC 1 cut(s) 19
BspPI GGATC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 2 cut(s) 362, 373
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 189, 331
BstC8I GCNNGC 1 cut(s) 12
BstKTI GATC 2 cut(s) 365, 376
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 2 cut(s) 362, 373
BstMWI GCNNNNNNNGC 2 cut(s) 142, 344
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 132, 304
BstV2I GAAGAC 1 cut(s) 25
BsuRI GGCC 3 cut(s) 115, 136, 338
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 327
Cac8I GCNNGC 1 cut(s) 12
CaiI CAGNNNCTG 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 114, 336
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 322
CviAII CATG 1 cut(s) 199
CviQI GTAC 1 cut(s) 322
DpnI GATC 2 cut(s) 364, 375
DpnII GATC 2 cut(s) 362, 373
Eco130I CCWWGG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 1 cut(s) 202
FaiI YATR 4 cut(s) 200, 279, 381, 460
FaqI GGGAC 1 cut(s) 198
FatI CATG 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 146, 318
Fsp4HI GCNGC 2 cut(s) 146, 318
FspBI CTAG 1 cut(s) 440
GluI GCNGC 2 cut(s) 146, 318
HaeIII GGCC 3 cut(s) 115, 136, 338
HapII CCGG 2 cut(s) 15, 339
Hin1II CATG 1 cut(s) 202
HinfI GANTC 1 cut(s) 30
HpaII CCGG 2 cut(s) 15, 339
HphI GGTGA 1 cut(s) 246
Hpy188I TCNGA 3 cut(s) 212, 222, 309
HpyAV CCTTC 1 cut(s) 171
HpyCH4III ACNGT 2 cut(s) 189, 331
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 2 cut(s) 77, 148
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 344
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 1 cut(s) 202
Kzo9I GATC 2 cut(s) 362, 373
Lsp1109I GCAGC 2 cut(s) 132, 304
LweI GCATC 1 cut(s) 86
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 1 cut(s) 440
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 325, 357
MalI GATC 2 cut(s) 364, 375
MboI GATC 2 cut(s) 362, 373
MboII GAAGA 3 cut(s) 25, 72, 400
MfeI CAATTG 1 cut(s) 300
MluCI AATT 3 cut(s) 149, 300, 426
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 1 cut(s) 105
MseI TTAA 3 cut(s) 60, 215, 243
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 2 cut(s) 15, 339
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 142, 344
NdeII GATC 2 cut(s) 362, 373
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 1 cut(s) 325
NspI RCATGY 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 440
PciI ACATGT 1 cut(s) 198
Pfl23II CGTACG 1 cut(s) 321
PkrI GCNGC 2 cut(s) 147, 319
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PscI ACATGT 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspLI CGTACG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 2 cut(s) 114, 336
PstNI CAGNNNCTG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 3 cut(s) 60, 215, 243
SatI GCNGC 2 cut(s) 146, 318
Sau3AI GATC 2 cut(s) 362, 373
Sau96I GGNCC 2 cut(s) 114, 336
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 164
SexAI ACCWGGT 1 cut(s) 162
SfaNI GCATC 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 440
Sse9I AATT 3 cut(s) 149, 300, 426
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 189, 331
TaiI ACGT 1 cut(s) 327
TaqI TCGA 1 cut(s) 392
TasI AATT 3 cut(s) 149, 300, 426
Tru1I TTAA 3 cut(s) 60, 215, 243
Tru9I TTAA 3 cut(s) 60, 215, 243
TscAI CASTG 2 cut(s) 73, 334
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 145, 317
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 400
TspRI CASTG 2 cut(s) 73, 334
VspI ATTAAT 1 cut(s) 243
XceI RCATGY 1 cut(s) 202
XspI CTAG 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.