Rw7G021540

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0024645
Physical Location & Seq
Reverse (-)
25009266 .. 25010341
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021540.1

Sequence Viewer

Length: 462 bp
ATGTCGTTGCTTGCTGGTTCCCTTTTCGGAGTCTTCAAAGGTGTAGCTTTGGTGGTGTTCAATGCCACTGCTGGTGCTTCTTCTTGCTATTTCTTGTCAAAGGTGATAGGGAGGCCCCTTGCCACTTCTCTTTGGCCCGACAAGCTCCTGTTTTTCCAAAACCAGGTGGCTAAAAGAAGACAAGGGTTGCTGAACTACATGTTGTTTCTCCGATTAACTCCAACCTTGCCAAACACATTTATTAATGTTGCTTCACCAATAGTCGATGTGCCTTATCATATTTTCTTCCTGGCAACCTCAATTGGTCTGATACCTGCTGCTTATGTCACTGTCAGGGCTGGACTAGCTCTTGGAGAATTGCGATCTCTTGGGGATCTCTATGACTTCAACTCAATAGCCACCTTGTTCTTCATCGGAGTTGTCTCAGTTACACCCACATTAGTGAGCAAGAACAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

153

Amino Acids

16.57

Weight (kDa)

9.93

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 2 - 114 3.6e-14 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024645)

Species Orthologous Gene IDs
pyrus_communis pycom06g03040
rosa_multiflora Rmu_sc0019437.1_g000001
rosa_wichuraiana Rw7G021540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 322
AclWI GGATC 1 cut(s) 381
AfiI CCNNNNNNNGG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 3 cut(s) 37, 61, 388
AjnI CCWGG 2 cut(s) 162, 288
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 3 cut(s) 47, 145, 347
AluI AGCT 3 cut(s) 47, 145, 347
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 1 cut(s) 381
AoxI GGCC 2 cut(s) 113, 134
ApeKI GCWGC 1 cut(s) 317
AseI ATTAAT 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 114, 135
AsuHPI GGTGA 2 cut(s) 115, 246
BbsI GAAGAC 2 cut(s) 25, 184
BbvI GCAGC 1 cut(s) 304
BciT130I CCWGG 2 cut(s) 164, 290
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 322
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
Bme1390I CCNGG 2 cut(s) 164, 290
BmgT120I GGNCC 2 cut(s) 114, 135
BmiI GGNNCC 2 cut(s) 19, 116
BmrFI CCNGG 2 cut(s) 164, 290
BpiI GAAGAC 2 cut(s) 25, 184
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseBI CCWGG 2 cut(s) 164, 290
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 438
BseXI GCAGC 1 cut(s) 304
BshFI GGCC 2 cut(s) 115, 136
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 427
BsnI GGCC 2 cut(s) 115, 136
Bsp143I GATC 2 cut(s) 362, 373
BspANI GGCC 2 cut(s) 115, 136
BspCNI CTCAG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 19, 116
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 1 cut(s) 381
BssMI GATC 2 cut(s) 362, 373
Bst2UI CCWGG 2 cut(s) 164, 290
Bst4CI ACNGT 1 cut(s) 331
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 424
BstKTI GATC 2 cut(s) 365, 376
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 2 cut(s) 362, 373
BstMWI GCNNNNNNNGC 2 cut(s) 142, 344
BstNI CCWGG 2 cut(s) 164, 290
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 162, 288
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 2 cut(s) 25, 184
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 2 cut(s) 115, 136
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 327
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 12
Cfr13I GGNCC 2 cut(s) 114, 135
CsiI ACCWGGT 1 cut(s) 162
CviAII CATG 1 cut(s) 199
CviJI RGCY 8 cut(s) 47, 115, 136, 145, 170, 338, 347, 398
CviKI_1 RGCY 8 cut(s) 47, 115, 136, 145, 170, 338, 347, 398
DdeI CTNAG 1 cut(s) 424
DpnI GATC 2 cut(s) 364, 375
DpnII GATC 2 cut(s) 362, 373
EcoO109I RGGNCCY 1 cut(s) 114
EcoRII CCWGG 2 cut(s) 162, 288
FaeI CATG 1 cut(s) 202
FaiI YATR 5 cut(s) 200, 279, 324, 381, 460
FatI CATG 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 318
Fsp4HI GCNGC 1 cut(s) 318
FspBI CTAG 1 cut(s) 344
GluI GCNGC 1 cut(s) 318
HaeIII GGCC 2 cut(s) 115, 136
Hin1II CATG 1 cut(s) 202
HinfI GANTC 1 cut(s) 30
HphI GGTGA 2 cut(s) 115, 246
Hpy188I TCNGA 4 cut(s) 29, 212, 309, 416
HpyCH4III ACNGT 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 344
HpyF3I CTNAG 1 cut(s) 424
Hsp92II CATG 1 cut(s) 202
Kzo9I GATC 2 cut(s) 362, 373
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 9 cut(s) 57, 149, 161, 176, 275, 302, 319, 324, 327
Lsp1109I GCAGC 1 cut(s) 304
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 1 cut(s) 344
MaeIII GTNAC 2 cut(s) 325, 427
MalI GATC 2 cut(s) 364, 375
MboI GATC 2 cut(s) 362, 373
MboII GAAGA 5 cut(s) 25, 72, 189, 277, 400
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 1 cut(s) 373
MluCI AATT 2 cut(s) 300, 356
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 2 cut(s) 105, 307
MseI TTAA 2 cut(s) 215, 243
MslI CAYNNNNRTG 1 cut(s) 440
MspR9I CCNGG 2 cut(s) 164, 290
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 2 cut(s) 164, 290
MwoI GCNNNNNNNGC 2 cut(s) 142, 344
NdeII GATC 2 cut(s) 362, 373
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 2 cut(s) 19, 116
NmuCI GTSAC 1 cut(s) 325
NspI RCATGY 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 440
PciI ACATGT 1 cut(s) 198
PkrI GCNGC 1 cut(s) 319
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PscI ACATGT 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 162, 288
PspGI CCWGG 2 cut(s) 162, 288
PspN4I GGNNCC 2 cut(s) 19, 116
PspPI GGNCC 2 cut(s) 114, 135
PsuI RGATCY 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 2 cut(s) 215, 243
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 2 cut(s) 362, 373
Sau96I GGNCC 2 cut(s) 114, 135
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 2 cut(s) 164, 290
SexAI ACCWGGT 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 440
Sse9I AATT 2 cut(s) 300, 356
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 162, 288
TaaI ACNGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 300, 356
Tru1I TTAA 2 cut(s) 215, 243
Tru9I TTAA 2 cut(s) 215, 243
TscAI CASTG 2 cut(s) 73, 334
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 400
TspRI CASTG 2 cut(s) 73, 334
VspI ATTAAT 1 cut(s) 243
XceI RCATGY 1 cut(s) 202
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.