pycom06g03690

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0029297
Physical Location & Seq
Forward (+)
4664447 .. 4668907
4461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03690.4

Sequence Viewer

Length: 2094 bp
ATGAAATTTTTTTCTTTAAAGTCGATCGATCCAGACTACTACTCAGTTGAATTAATTGTGACGGAGCTGGGCAAAGGGGTCCCTCTTGGATTATTTTCAACCCCTTTAAAGAAGATTGCGGGGATCATCCATGATAATTCTTACCTGAATGATAGCGTGAACAAATGGACCTGGGTTGAATTATCCTCAACAGAATCTACGGGATATAATGGTGAAAAGTCGTGTGGCAAAATCAGATGTTCAGTGCTGTTGTCACCGACATCTGAAGCTGAGATAAGTGATCAAACTGATAACAGTGGCAGAAAATCTGGTTTTATTCAGATTAGTCCAAGCAGGGAAGGGCCTTGGACTACAGTGAGGCTAAATTATGCTGCACCAGCTGCTTGTTGGAGGTTAGGGAATGACGTTGTTGCTAGTGAAGTACATGTGAATGATGGCAATCGATATGTGGATATTAGGTCTCTTGTTTCCGTACGTAACAGTACGGATTTTGAACTTGATTTGTGCCTTGTACCAAAAGTTTCCATGGAAGATACCACTCTAAAAAACAACGCCAGTACCCTTGAAGGTCAAATACAATTTAAGAAACTTCAGACTGATGAGTTCTTTGAAACTGAAAAATACAGCCCCGGTACTGGTTGGATTGGCACCATTGCTCAACCAAGCCAAGGTATCATTGAAAGTGGAGGATCTCATCAGGGAGTTCCGGCAGTTGAGTTACCACTGGGCTGGGAATGGGTTGATGATTGGCATTTGAATATGGAATCTGTTCACACTGCTGATGGTTGGGTTTATGCTCCAGATGTTGAGAGTTTAAAATGGCCCGAGTCATTTGATCCATTAAGATCTGTAAACTCTGCAAGGCAAAGGAGATGGATCAGGAATAGAAAACAAAATGATACACATCAGAAAATATCTATTGGACTTTTGAAACCTGGAGATACGGTATCCCTTCCTCTATCTGGGTTAACACAACCTGAAATGTACGTATTGTGTTTAAGGCCCTCTCTCTGCAATAGTAGTGAGTATTCTTGGAGTTCAGTGGTGGATGGGTCTGAACAAACAGAGGATTCTGGTAAGTCCAAAGTATGCTCTGGGATAAGTGTGTCTTCTCTCACCGAGTTGGAAGAACTGTTATATTGCACTCAGATAAGTGGTAGTTCTTCAAGTTCTCATAAGTTGTGGTTTTGTATAAGTGTACAAGCGACGGAGATTGCCAAAGATATTCATTCTGATCCTATCCAGGATTGGACTCTTGTAATCAAGTCCCCGCTATCTATCTCCAATTTTCTTCCGCTTGCAGCTGAATATTCTGTTTTTGAGATGCAAGATAGTGGTAACTTTTTTGCCTGCTCGAGAGGAGTATTATTTTCAGGAAAAAGTGTCAATGTTTATAGTGCTGACATAAGAAGCCCTTTGTTCCTTTCACTGCTTCCGCAAAGAGGGTGGCTGCCTATACATGAAGCTGTTCTTTTTTCCCATCCTCATGAAGTTCCACCGAAAACTATAAGTTTGAGAAGTTCGATATCCGGAAGGATTGTTCAAATTGTTCTTGAACAGAATTCTGACAAAGAACGGCCACTGCAAGCTAAGATCATTAGGCTGTATTCTCCTTATTGGTATTCAATTTCTTGGTGTCCCCCTCTCAAACTTAGGCTTCTAGATATCAAAGGAAAGAAACATATGCGAAAGGTTGGTAATCCATTTCGTTCTACAAAGGACAATGAAACTATCCTGGAGGAAATAACTGAAGAGGAAATATATGAAGGTCATACAATTGCTTCACCTTTGAAATTTAAATTGTTGGGCCTTGCGTTGTCCATTGATCAATCTGGAAAGGAGCAATTTGGACCTGTCAAAGATCTTTCTCCCCTTGATGACACGGATGGGTCGTTGGATCTCTATGCTCATGATAATGAAGGCAGTTGTATGCGGATTTTTATTACAACAAAACCATGCCTATATCAATCTGTTCCCACAAAGGTAATTTCTGTCCGACCATATATGACTTTTACCAATAGAATTGGTCGGGACATATCCATTAAGTTATGCAGTGAGGATGATATTTGTACGATAGATCCTTCGAGCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

698

Amino Acids

77.75

Weight (kDa)

5.42

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029297)

Species Orthologous Gene IDs
pyrus_communis pycom06g03690
rosa_multiflora Rmu_co8403995.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccIII TCCGGA 1 cut(s) 1529
AciI CCGC 5 cut(s) 119, 1271, 1295, 1436, 1933
AclWI GGATC 8 cut(s) 23, 131, 697, 830, 884, 1229, 1905, 2072
AcoI YGGCCR 1 cut(s) 1577
AcsI RAATTY 3 cut(s) 5, 1561, 1793
AcuI CTGAAG 3 cut(s) 285, 575, 1770
AfaI GTAC 9 cut(s) 423, 474, 484, 513, 559, 634, 986, 1200, 2071
AfiI CCNNNNNNNGG 4 cut(s) 635, 668, 962, 1442
AflIII ACRYGT 1 cut(s) 424
AjnI CCWGG 4 cut(s) 170, 934, 1242, 1734
AloI GAACNNNNNNTCC 2 cut(s) 1524, 1556
AluBI AGCT 6 cut(s) 67, 269, 380, 1304, 1466, 1589
AluI AGCT 6 cut(s) 67, 269, 380, 1304, 1466, 1589
Alw26I GTCTC 1 cut(s) 465
AlwI GGATC 8 cut(s) 23, 131, 697, 830, 884, 1229, 1905, 2072
Ama87I CYCGRG 2 cut(s) 824, 1354
Aor13HI TCCGGA 1 cut(s) 1529
AoxI GGCC 5 cut(s) 341, 821, 1001, 1577, 1807
ApeKI GCWGC 4 cut(s) 371, 380, 1301, 1450
ApoI RAATTY 3 cut(s) 5, 1561, 1793
AseI ATTAAT 1 cut(s) 53
Asp700I GAANNNNTTC 2 cut(s) 768, 1467
AspS9I GGNCC 7 cut(s) 79, 168, 341, 822, 1002, 1807, 1850
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 4 cut(s) 224, 246, 1108, 1776
AvaI CYCGRG 2 cut(s) 824, 1354
AvaII GGWCC 3 cut(s) 79, 168, 1850
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BanI GGYRCC 1 cut(s) 647
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 1 cut(s) 1101
BbvI GCAGC 4 cut(s) 358, 367, 1313, 1437
BccI CCATC 6 cut(s) 428, 776, 867, 1043, 1488, 1880
BceAI ACGGC 1 cut(s) 1592
BciT130I CCWGG 4 cut(s) 172, 936, 1244, 1736
BciVI GTATCC 1 cut(s) 958
BclI TGATCA 2 cut(s) 280, 1825
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 2 cut(s) 414, 1661
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 958
BglII AGATCT 2 cut(s) 845, 1861
BisI GCNGC 4 cut(s) 372, 381, 1302, 1451
BlsI GCNGC 4 cut(s) 373, 382, 1303, 1452
Bme1390I CCNGG 5 cut(s) 172, 630, 936, 1244, 1736
Bme18I GGWCC 3 cut(s) 79, 168, 1850
BmeT110I CYCGRG 2 cut(s) 824, 1354
BmgT120I GGNCC 7 cut(s) 79, 168, 341, 822, 1002, 1807, 1850
BmiI GGNNCC 3 cut(s) 80, 81, 649
BmrFI CCNGG 5 cut(s) 172, 630, 936, 1244, 1736
BmrI ACTGGG 1 cut(s) 734
BmsI GCATC 1 cut(s) 1314
BmuI ACTGGG 1 cut(s) 734
BpiI GAAGAC 1 cut(s) 1101
BpmI CTGGAG 3 cut(s) 783, 957, 1757
BpuMI CCSGG 1 cut(s) 630
Bsa29I ATCGAT 2 cut(s) 27, 442
BsaAI YACGTR 2 cut(s) 476, 988
BsaBI GATNNNNATC 2 cut(s) 438, 903
BsaI GGTCTC 1 cut(s) 465
BsaJI CCNNGG 5 cut(s) 171, 344, 525, 628, 667
BsaWI WCCGGW 1 cut(s) 1529
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 4 cut(s) 635, 668, 962, 1442
Bse1I ACTGG 3 cut(s) 555, 640, 729
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 2 cut(s) 438, 903
BseAI TCCGGA 1 cut(s) 1529
BseBI CCWGG 4 cut(s) 172, 936, 1244, 1736
BseCI ATCGAT 2 cut(s) 27, 442
BseDI CCNNGG 5 cut(s) 171, 344, 525, 628, 667
BseGI GGATG 5 cut(s) 126, 1054, 1480, 1891, 2065
BseJI GATNNNNATC 2 cut(s) 438, 903
BseLI CCNNNNNNNGG 4 cut(s) 635, 668, 962, 1442
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 3 cut(s) 57, 261, 1160
BseNI ACTGG 3 cut(s) 555, 640, 729
BseRI GAGGAG 1 cut(s) 1374
BseXI GCAGC 4 cut(s) 358, 367, 1313, 1437
BseYI CCCAGC 2 cut(s) 67, 729
BsgI GTGCAG 1 cut(s) 357
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 5 cut(s) 343, 823, 1003, 1579, 1809
BshNI GGYRCC 1 cut(s) 647
BshVI ATCGAT 2 cut(s) 27, 442
BsiEI CGRYCG 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 824, 1354
BsiSI CCGG 3 cut(s) 630, 707, 1530
BsiWI CGTACG 1 cut(s) 472
BslFI GGGAC 4 cut(s) 65, 1252, 1623, 2045
BslI CCNNNNNNNGG 4 cut(s) 635, 668, 962, 1442
BsmAI GTCTC 1 cut(s) 465
BsmFI GGGAC 4 cut(s) 65, 1252, 1623, 2045
BsnI GGCC 5 cut(s) 343, 823, 1003, 1579, 1809
Bso31I GGTCTC 1 cut(s) 465
BsoBI CYCGRG 2 cut(s) 824, 1354
Bsp13I TCCGGA 1 cut(s) 1529
Bsp1407I TGTACA 1 cut(s) 1198
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 5 cut(s) 119, 1271, 1295, 1436, 1933
BspANI GGCC 5 cut(s) 343, 823, 1003, 1579, 1809
BspCNI CTCAG 3 cut(s) 56, 262, 1159
BspDI ATCGAT 2 cut(s) 27, 442
BspEI TCCGGA 1 cut(s) 1529
BspHI TCATGA 2 cut(s) 1486, 1909
BspLI GGNNCC 3 cut(s) 80, 81, 649
BspPI GGATC 8 cut(s) 23, 131, 697, 830, 884, 1229, 1905, 2072
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 1198
BsrI ACTGG 3 cut(s) 555, 640, 729
BssECI CCNNGG 5 cut(s) 171, 344, 525, 628, 667
BssT1I CCWWGG 3 cut(s) 344, 525, 667
Bst2UI CCWGG 4 cut(s) 172, 936, 1244, 1736
Bst4CI ACNGT 5 cut(s) 296, 355, 482, 946, 1134
Bst6I CTCTTC 1 cut(s) 1746
BstAPI GCANNNNNTGC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 1198
BstBAI YACGTR 2 cut(s) 476, 988
BstC8I GCNNGC 3 cut(s) 1299, 1351, 1587
BstDEI CTNAG 5 cut(s) 43, 270, 1146, 1590, 1652
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 5 cut(s) 126, 1054, 1480, 1891, 2065
BstMAI GTCTC 1 cut(s) 465
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 377, 380
BstNI CCWGG 4 cut(s) 172, 936, 1244, 1736
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 5 cut(s) 170, 628, 934, 1242, 1734
BstSFI CTRYAG 1 cut(s) 351
BstSNI TACGTA 2 cut(s) 476, 988
BstV1I GCAGC 4 cut(s) 358, 367, 1313, 1437
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 5 cut(s) 689, 845, 1861, 1897, 2077
BstXI CCANNNNNNTGG 1 cut(s) 729
BstYI RGATCY 5 cut(s) 689, 845, 1861, 1897, 2077
Bsu15I ATCGAT 2 cut(s) 27, 442
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 5 cut(s) 343, 823, 1003, 1579, 1809
BsuTUI ATCGAT 2 cut(s) 27, 442
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 5 cut(s) 126, 1054, 1480, 1891, 2065
BtsI GCAGTG 4 cut(s) 774, 1427, 1580, 2059
BtsIMutI CAGTG 9 cut(s) 249, 301, 360, 722, 774, 1047, 1427, 1580, 2059
Cac8I GCNNGC 3 cut(s) 1299, 1351, 1587
CciI TCATGA 2 cut(s) 1486, 1909
Cfr13I GGNCC 7 cut(s) 79, 168, 341, 822, 1002, 1807, 1850
ClaI ATCGAT 2 cut(s) 27, 442
Csp6I GTAC 9 cut(s) 422, 473, 483, 512, 558, 633, 985, 1199, 2070
CspCI CAANNNNNGTGG 2 cut(s) 1427, 1462
CviAII CATG 7 cut(s) 131, 425, 526, 1460, 1487, 1910, 1956
CviQI GTAC 9 cut(s) 422, 473, 483, 512, 558, 633, 985, 1199, 2070
DdeI CTNAG 5 cut(s) 43, 270, 1146, 1590, 1652
DraI TTTAAA 4 cut(s) 18, 108, 816, 1798
EaeI YGGCCR 1 cut(s) 1577
Eam1104I CTCTTC 1 cut(s) 1746
EarI CTCTTC 1 cut(s) 1746
Eco105I TACGTA 2 cut(s) 476, 988
Eco130I CCWWGG 3 cut(s) 344, 525, 667
Eco31I GGTCTC 1 cut(s) 465
Eco32I GATATC 2 cut(s) 1527, 1666
Eco47I GGWCC 3 cut(s) 79, 168, 1850
Eco57I CTGAAG 3 cut(s) 285, 575, 1770
Eco88I CYCGRG 2 cut(s) 824, 1354
EcoO109I RGGNCCY 3 cut(s) 79, 341, 1002
EcoRI GAATTC 1 cut(s) 1561
EcoRII CCWGG 4 cut(s) 170, 934, 1242, 1734
EcoRV GATATC 2 cut(s) 1527, 1666
EcoT14I CCWWGG 3 cut(s) 344, 525, 667
ErhI CCWWGG 3 cut(s) 344, 525, 667
FaeI CATG 7 cut(s) 134, 428, 529, 1463, 1490, 1913, 1959
FalI AAGNNNNNCTT 6 cut(s) 1093, 1125, 1399, 1431, 1455, 1487
FaqI GGGAC 4 cut(s) 65, 1252, 1623, 2045
FatI CATG 7 cut(s) 130, 424, 525, 1459, 1486, 1909, 1955
FauI CCCGC 2 cut(s) 112, 1278
FauNDI CATATG 1 cut(s) 1683
FbaI TGATCA 2 cut(s) 280, 1825
Fnu4HI GCNGC 4 cut(s) 372, 381, 1302, 1451
FokI GGATG 5 cut(s) 113, 1061, 1467, 1898, 2072
Fsp4HI GCNGC 4 cut(s) 372, 381, 1302, 1451
FspBI CTAG 2 cut(s) 414, 1661
GluI GCNGC 4 cut(s) 372, 381, 1302, 1451
GsaI CCCAGC 2 cut(s) 71, 733
GsuI CTGGAG 3 cut(s) 783, 957, 1757
HaeIII GGCC 5 cut(s) 343, 823, 1003, 1579, 1809
HapII CCGG 3 cut(s) 630, 707, 1530
Hin1II CATG 7 cut(s) 134, 428, 529, 1463, 1490, 1913, 1959
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HinfI GANTC 5 cut(s) 194, 764, 827, 1070, 1252
HpaI GTTAAC 1 cut(s) 969
HpaII CCGG 3 cut(s) 630, 707, 1530
HphI GGTGA 4 cut(s) 224, 246, 1108, 1776
Hpy166II GTNNAC 5 cut(s) 160, 772, 853, 969, 1199
Hpy8I GTNNAC 5 cut(s) 160, 772, 853, 969, 1199
Hpy99I CGWCG 1 cut(s) 1210
HpyAV CCTTC 7 cut(s) 332, 560, 962, 1527, 1760, 1913, 2091
HpyCH4III ACNGT 5 cut(s) 296, 355, 482, 946, 1134
HpyCH4IV ACGT 3 cut(s) 405, 475, 987
HpyCH4V TGCA 8 cut(s) 374, 860, 1014, 1143, 1301, 1327, 1585, 2052
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 380
HpyF3I CTNAG 5 cut(s) 43, 270, 1146, 1590, 1652
HpySE526I ACGT 3 cut(s) 405, 475, 987
Hsp92II CATG 7 cut(s) 134, 428, 529, 1463, 1490, 1913, 1959
KflI GGGWCCC 1 cut(s) 79
Kpn2I TCCGGA 1 cut(s) 1529
Ksp22I TGATCA 2 cut(s) 280, 1825
KspAI GTTAAC 1 cut(s) 969
LmnI GCTCC 3 cut(s) 64, 802, 1840
Lsp1109I GCAGC 4 cut(s) 358, 367, 1313, 1437
LweI GCATC 1 cut(s) 1314
MaeI CTAG 2 cut(s) 414, 1661
MaeII ACGT 3 cut(s) 405, 475, 987
MaeIII GTNAC 5 cut(s) 58, 252, 476, 717, 1337
MboII GAAGA 7 cut(s) 124, 542, 1101, 1139, 1155, 1283, 1763
MfeI CAATTG 1 cut(s) 1776
MflI RGATCY 5 cut(s) 689, 845, 1861, 1897, 2077
MlyI GAGTC 2 cut(s) 836, 1246
MmeI TCCRAC 5 cut(s) 368, 620, 1104, 1875, 2020
MroI TCCGGA 1 cut(s) 1529
MroXI GAANNNNTTC 2 cut(s) 768, 1467
MslI CAYNNNNRTG 3 cut(s) 429, 1485, 1914
MspA1I CMGCKG 2 cut(s) 380, 1304
MspI CCGG 3 cut(s) 630, 707, 1530
MspR9I CCNGG 5 cut(s) 172, 630, 936, 1244, 1736
MunI CAATTG 1 cut(s) 1776
MvaI CCWGG 4 cut(s) 172, 936, 1244, 1736
MwoI GCNNNNNNNGC 2 cut(s) 377, 380
NciI CCSGG 1 cut(s) 630
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 1 cut(s) 1683
NlaIII CATG 7 cut(s) 134, 428, 529, 1463, 1490, 1913, 1959
NlaIV GGNNCC 3 cut(s) 80, 81, 649
NmuCI GTSAC 2 cut(s) 58, 252
NspI RCATGY 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 1354
PagI TCATGA 2 cut(s) 1486, 1909
PciI ACATGT 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 1889
PdmI GAANNNNTTC 2 cut(s) 768, 1467
PfeI GAWTC 3 cut(s) 194, 764, 1070
Pfl23II CGTACG 1 cut(s) 472
PfoI TCCNGGA 2 cut(s) 1242, 1734
PkrI GCNGC 4 cut(s) 373, 382, 1303, 1452
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 2 cut(s) 835, 1246
PpsI GAGTC 2 cut(s) 835, 1246
Ppu21I YACGTR 2 cut(s) 476, 988
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 53
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 4 cut(s) 170, 934, 1242, 1734
PspFI CCCAGC 2 cut(s) 67, 729
PspGI CCWGG 4 cut(s) 170, 934, 1242, 1734
PspLI CGTACG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 80, 81, 649
PspPI GGNCC 7 cut(s) 79, 168, 341, 822, 1002, 1807, 1850
PspPPI RGGWCCY 1 cut(s) 79
PsuI RGATCY 5 cut(s) 689, 845, 1861, 1897, 2077
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 2 cut(s) 380, 1304
RsaI GTAC 9 cut(s) 423, 474, 484, 513, 559, 634, 986, 1200, 2071
RsaNI GTAC 9 cut(s) 422, 473, 483, 512, 558, 633, 985, 1199, 2070
RseI CAYNNNNRTG 3 cut(s) 429, 1485, 1914
SatI GCNGC 4 cut(s) 372, 381, 1302, 1451
Sau96I GGNCC 7 cut(s) 79, 168, 341, 822, 1002, 1807, 1850
SchI GAGTC 2 cut(s) 836, 1246
ScrFI CCNGG 5 cut(s) 172, 630, 936, 1244, 1736
SfaNI GCATC 1 cut(s) 1314
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 1354
SinI GGWCC 3 cut(s) 79, 168, 1850
SlaI CTCGAG 1 cut(s) 1354
SmiI ATTTAAAT 1 cut(s) 1798
SmiMI CAYNNNNRTG 3 cut(s) 429, 1485, 1914
SmlI CTYRAG 1 cut(s) 1354
SmoI CTYRAG 1 cut(s) 1354
SnaBI TACGTA 2 cut(s) 476, 988
SsiI CCGC 5 cut(s) 119, 1271, 1295, 1436, 1933
SspI AATATT 1 cut(s) 1310
SspMI CTAG 2 cut(s) 414, 1661
StyD4I CCNGG 5 cut(s) 170, 628, 934, 1242, 1734
StyI CCWWGG 3 cut(s) 344, 525, 667
SwaI ATTTAAAT 1 cut(s) 1798
TaaI ACNGT 5 cut(s) 296, 355, 482, 946, 1134
TaiI ACGT 3 cut(s) 408, 478, 990
TaqI TCGA 6 cut(s) 23, 27, 442, 1355, 1523, 2084
TatI WGTACW 2 cut(s) 421, 1198
TfiI GAWTC 3 cut(s) 194, 764, 1070
TscAI CASTG 9 cut(s) 249, 301, 360, 729, 781, 1047, 1434, 1587, 2059
TseFI GTSAC 2 cut(s) 58, 252
TseI GCWGC 4 cut(s) 371, 380, 1301, 1450
Tsp45I GTSAC 2 cut(s) 58, 252
TspDTI ATGAA 7 cut(s) 17, 1217, 1476, 1503, 1740, 1779, 1932
TspGWI ACGGA 5 cut(s) 77, 460, 500, 1223, 1898
TspRI CASTG 9 cut(s) 249, 301, 360, 729, 781, 1047, 1434, 1587, 2059
VpaK11BI GGWCC 3 cut(s) 79, 168, 1850
VspI ATTAAT 1 cut(s) 53
XapI RAATTY 3 cut(s) 5, 1561, 1793
XbaI TCTAGA 1 cut(s) 1660
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 384
XhoI CTCGAG 1 cut(s) 1354
XmnI GAANNNNTTC 2 cut(s) 768, 1467
XspI CTAG 2 cut(s) 414, 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.