Rmu_co8403995.1_g000001

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8403995.1
OG0029297
Physical Location & Seq
Reverse (-)
2 .. 1247
1246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8403995.1_g000001.1.cds

Sequence Viewer

Length: 1034 bp
atggcatctattgacactgctgatggctgggtctatgctccagatattgaaagtctaaaatggcccgagtcatttgatccatcacggtttgtgaactatgcaaggcaaaggagatggatcaggaataggaagcaaagtactacgaatcaggaaatatatgttggaactttgaaacctggagataccataccccttcctctatgtgggttgacacaacctggattgtatgtcttgcgtttaaggccttctgatcttagcaatcatgatgagtattcttggagttcagcggtggatgggtctgaacaaccagaagagtctggttcgtcaaaagtatgtcctgagataagtgtatcgtctcttattgagtcagagaaactgttgtattgctcacagataagtagtacttcttcaagtggttcccacaaattgtggttttgtatgagtatacaagcgacagaaattgcaaaagacattcattctgatcctatccaggattggaatcttgtggttaagtccccgctatctatctccaattttcttccccttgcagctgagttttctgttcttgagatgcaagaaaatggtggttttgttgcctgcttcagaggggtgttttttccaggaaagaccgtgaatgtttttagtgctgacataagaaaacctttatacttttcactgcttccacaaagaggatggttgcccatccatgaagctgtccttttgtcccatcctcatgaagtttcatcaaaaacaataaacttgagaagttcgatatctggaaggattgttcaaattattcttgagcagaattccgttgaagagcgtccactgcaggctaagatcattagactctatgctccttattggttttcaattgctaggtgtcctcctctcacattcaggcttgtagatatagaagggaagaaaaatacacgaaagataggtggtttatttcagtctaaaaagattaatgaagttgtcctagaggaaataactgaagaggaaatatatgaaggtcatacaattgcttcagc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

345

Amino Acids

38.82

Weight (kDa)

5.88

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029297)

Species Orthologous Gene IDs
pyrus_communis pycom06g03690
rosa_multiflora Rmu_co8403995.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 445
AciI CCGC 2 cut(s) 287, 518
AclWI GGATC 3 cut(s) 71, 125, 476
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 3 cut(s) 586, 1014, 1017
AfaI GTAC 2 cut(s) 139, 403
AfiI CCNNNNNNNGG 2 cut(s) 203, 689
AgsI TTSAA 6 cut(s) 50, 172, 411, 791, 818, 873
AjnI CCWGG 4 cut(s) 175, 217, 489, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
AluBI AGCT 2 cut(s) 551, 713
AluI AGCT 2 cut(s) 551, 713
Alw26I GTCTC 1 cut(s) 360
AlwI GGATC 3 cut(s) 71, 125, 476
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 2 cut(s) 62, 242
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 808
AseI ATTAAT 1 cut(s) 969
AspS9I GGNCC 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 65
BarI GAAGNNNNNNTAC 2 cut(s) 391, 423
BbvI GCAGC 1 cut(s) 560
BccI CCATC 7 cut(s) 17, 88, 108, 287, 687, 710, 735
BciT130I CCWGG 4 cut(s) 177, 219, 491, 621
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 2 cut(s) 879, 983
BfmI CTRYAG 1 cut(s) 830
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
BmcAI AGTACT 2 cut(s) 139, 403
Bme1390I CCNGG 4 cut(s) 177, 219, 491, 621
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 4 cut(s) 177, 219, 491, 621
BmsI GCATC 2 cut(s) 14, 561
BpmI CTGGAG 2 cut(s) 24, 198
BpuEI CTTGAG 3 cut(s) 587, 781, 821
BsaBI GATNNNNATC 1 cut(s) 498
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 689
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 4 cut(s) 177, 219, 491, 621
BseGI GGATG 4 cut(s) 298, 698, 702, 727
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 203, 689
BseMII CTCAG 2 cut(s) 330, 543
BseRI GAGGAG 1 cut(s) 879
BseXI GCAGC 1 cut(s) 560
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 2 cut(s) 64, 244
BsiHKCI CYCGRG 1 cut(s) 65
BslFI GGGAC 2 cut(s) 499, 709
BslI CCNNNNNNNGG 2 cut(s) 203, 689
BsmAI GTCTC 1 cut(s) 360
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 2 cut(s) 499, 709
BsnI GGCC 2 cut(s) 64, 244
BsoBI CYCGRG 1 cut(s) 65
Bsp143I GATC 5 cut(s) 76, 117, 250, 481, 840
BspACI CCGC 2 cut(s) 287, 518
BspANI GGCC 2 cut(s) 64, 244
BspCNI CTCAG 2 cut(s) 331, 544
BspHI TCATGA 2 cut(s) 262, 733
BspLI GGNNCC 1 cut(s) 418
BspMAI CTGCAG 1 cut(s) 834
BspPI GGATC 3 cut(s) 71, 125, 476
BspQI GCTCTTC 1 cut(s) 813
BssMI GATC 5 cut(s) 76, 117, 250, 481, 840
BssNAI GTATAC 1 cut(s) 446
Bst1107I GTATAC 1 cut(s) 446
Bst2UI CCWGG 4 cut(s) 177, 219, 491, 621
Bst4CI ACNGT 3 cut(s) 87, 378, 631
Bst6I CTCTTC 3 cut(s) 306, 813, 993
BstC8I GCNNGC 2 cut(s) 598, 834
BstDEI CTNAG 4 cut(s) 254, 339, 552, 837
BstF5I GGATG 4 cut(s) 298, 698, 702, 727
BstKTI GATC 5 cut(s) 79, 120, 253, 484, 843
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 5 cut(s) 76, 117, 250, 481, 840
BstMWI GCNNNNNNNGC 2 cut(s) 241, 829
BstNI CCWGG 4 cut(s) 177, 219, 491, 621
BstSCI CCNGG 4 cut(s) 175, 217, 489, 619
BstSFI CTRYAG 1 cut(s) 830
BstV1I GCAGC 1 cut(s) 560
BstZ17I GTATAC 1 cut(s) 446
BsuRI GGCC 2 cut(s) 64, 244
BtsCI GGATG 4 cut(s) 298, 698, 702, 727
BtsI GCAGTG 3 cut(s) 15, 674, 827
BtsIMutI CAGTG 3 cut(s) 15, 674, 827
Cac8I GCNNGC 2 cut(s) 598, 834
CciI TCATGA 2 cut(s) 262, 733
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 1 cut(s) 812
Csp6I GTAC 2 cut(s) 138, 402
CviAII CATG 3 cut(s) 263, 707, 734
CviJI RGCY 7 cut(s) 27, 64, 244, 551, 713, 836, 904
CviKI_1 RGCY 7 cut(s) 27, 64, 244, 551, 713, 836, 904
CviQI GTAC 2 cut(s) 138, 402
DdeI CTNAG 4 cut(s) 254, 339, 552, 837
DpnI GATC 5 cut(s) 78, 119, 252, 483, 842
DpnII GATC 5 cut(s) 76, 117, 250, 481, 840
Eam1104I CTCTTC 3 cut(s) 306, 813, 993
EarI CTCTTC 3 cut(s) 306, 813, 993
Eco147I AGGCCT 1 cut(s) 244
Eco32I GATATC 1 cut(s) 774
Eco57I CTGAAG 3 cut(s) 586, 1014, 1017
Eco88I CYCGRG 1 cut(s) 65
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 4 cut(s) 175, 217, 489, 619
EcoRV GATATC 1 cut(s) 774
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 3 cut(s) 266, 710, 737
FalI AAGNNNNNCTT 6 cut(s) 388, 420, 646, 678, 702, 734
FaqI GGGAC 2 cut(s) 499, 709
FatI CATG 3 cut(s) 262, 706, 733
FauI CCCGC 1 cut(s) 525
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 4 cut(s) 305, 689, 705, 714
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 2 cut(s) 879, 983
GluI GCNGC 1 cut(s) 549
GsaI CCCAGC 1 cut(s) 31
GsuI CTGGAG 2 cut(s) 24, 198
HaeIII GGCC 2 cut(s) 64, 244
HgaI GACGC 1 cut(s) 812
Hin1II CATG 3 cut(s) 266, 710, 737
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 6 cut(s) 68, 145, 314, 365, 499, 849
Hpy166II GTNNAC 4 cut(s) 94, 210, 446, 827
Hpy188I TCNGA 5 cut(s) 250, 301, 370, 481, 605
Hpy188III TCNNGA 9 cut(s) 41, 121, 149, 263, 338, 566, 734, 777, 800
Hpy8I GTNNAC 4 cut(s) 94, 210, 446, 827
HpyAV CCTTC 5 cut(s) 203, 255, 774, 911, 1007
HpyCH4III ACNGT 3 cut(s) 87, 378, 631
HpyCH4V TGCA 5 cut(s) 101, 464, 548, 574, 832
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 829
HpyF3I CTNAG 4 cut(s) 254, 339, 552, 837
Hsp92II CATG 3 cut(s) 266, 710, 737
Kzo9I GATC 5 cut(s) 76, 117, 250, 481, 840
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 2 cut(s) 43, 862
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 2 cut(s) 14, 561
MaeI CTAG 2 cut(s) 879, 983
MalI GATC 5 cut(s) 78, 119, 252, 483, 842
MboI GATC 5 cut(s) 76, 117, 250, 481, 840
MboII GAAGA 6 cut(s) 323, 399, 530, 830, 934, 1010
MfeI CAATTG 2 cut(s) 873, 1023
MluCI AATT 7 cut(s) 425, 459, 532, 792, 808, 873, 1023
MlyI GAGTC 4 cut(s) 77, 323, 374, 843
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 8 cut(s) 207, 599, 683, 741, 897, 900, 979, 994
MseI TTAA 3 cut(s) 239, 510, 969
MslI CAYNNNNRTG 1 cut(s) 732
MspA1I CMGCKG 2 cut(s) 287, 551
MspR9I CCNGG 4 cut(s) 177, 219, 491, 621
MunI CAATTG 2 cut(s) 873, 1023
MvaI CCWGG 4 cut(s) 177, 219, 491, 621
MwoI GCNNNNNNNGC 2 cut(s) 241, 829
NdeII GATC 5 cut(s) 76, 117, 250, 481, 840
NlaIII CATG 3 cut(s) 266, 710, 737
NlaIV GGNNCC 1 cut(s) 418
PagI TCATGA 2 cut(s) 262, 733
PceI AGGCCT 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 813
PfeI GAWTC 2 cut(s) 145, 499
PfoI TCCNGGA 2 cut(s) 489, 619
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 4 cut(s) 76, 322, 373, 843
PpsI GAGTC 4 cut(s) 76, 322, 373, 843
PshBI ATTAAT 1 cut(s) 969
Psp6I CCWGG 4 cut(s) 175, 217, 489, 619
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 4 cut(s) 175, 217, 489, 619
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 63
PstI CTGCAG 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 139, 403
RsaNI GTAC 2 cut(s) 138, 402
RseI CAYNNNNRTG 1 cut(s) 732
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 3 cut(s) 239, 510, 969
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 5 cut(s) 76, 117, 250, 481, 840
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 2 cut(s) 139, 403
SchI GAGTC 4 cut(s) 77, 323, 374, 843
ScrFI CCNGG 4 cut(s) 177, 219, 491, 621
SetI ASST 8 cut(s) 178, 220, 553, 664, 715, 884, 946, 1018
SfaNI GCATC 2 cut(s) 14, 561
SfcI CTRYAG 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 732
SmlI CTYRAG 3 cut(s) 566, 760, 800
SmoI CTYRAG 3 cut(s) 566, 760, 800
Sse9I AATT 7 cut(s) 425, 459, 532, 792, 808, 873, 1023
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 2 cut(s) 287, 518
SspMI CTAG 2 cut(s) 879, 983
StuI AGGCCT 1 cut(s) 244
StyD4I CCNGG 4 cut(s) 175, 217, 489, 619
TaaI ACNGT 3 cut(s) 87, 378, 631
TaqI TCGA 1 cut(s) 770
TasI AATT 7 cut(s) 425, 459, 532, 792, 808, 873, 1023
TatI WGTACW 2 cut(s) 137, 401
TfiI GAWTC 2 cut(s) 145, 499
Tru1I TTAA 3 cut(s) 239, 510, 969
Tru9I TTAA 3 cut(s) 239, 510, 969
TscAI CASTG 3 cut(s) 22, 681, 834
TseI GCWGC 1 cut(s) 548
TspDTI ATGAA 6 cut(s) 464, 723, 732, 750, 987, 1026
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 3 cut(s) 22, 681, 834
VspI ATTAAT 1 cut(s) 969
XapI RAATTY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 690
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 2 cut(s) 879, 983
ZrmI AGTACT 2 cut(s) 139, 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.