pycom06g03860

Decapping nuclease DXO homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0022543
Physical Location & Seq
Forward (+)
4793425 .. 4795312
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03860.1

Sequence Viewer

Length: 435 bp
ATGGGTGCTGAAATGGATTGCTGTGACTCGACTGATGATGGAAGGAGGTTTTATGTGGAATTAAAGACAAGCCGTGAGTTGGACAACCATAAAATAGAGTATGAATTTGAGAAAGAGAAGCTCCTCAAGTTTTGGATTCAATCATTCTTAGCAGGTGTTCCCTATATTGTCATCGGATTTAGGAACGATGCGGGTCAACTTGTCCGCACAGAGAGACTAAGAACCAAAGATATAACGCAAAGAGTGAAAATGAAGAACTACTGGCAGGGAGGAGTTTGCTTGGCATTTGCTGATGAGGTATTATGCTGGCTCTATGGGACCGTAAAAGACAATGAAGATTACATATTGCAGTTTACTCATCCTTACCAACGTTTGGAGCTCTTACAAGCCCAATCTTGCCCAGAAGAAATTACTAACCATGTTCAGCAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

145

Amino Acids

17.02

Weight (kDa)

5.23

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 1 - 131 1.9e-40 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022543)

Species Orthologous Gene IDs
pyrus_communis pycom06g03860
rosa_laevigata RLG00000003315
rosa_multiflora Rmu_sc0003652.1_g000018
rosa_samantha Rh7AG228600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 373
AciI CCGC 2 cut(s) 191, 205
AclI AACGTT 1 cut(s) 370
AcsI RAATTY 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 79, 373
AgsI TTSAA 1 cut(s) 140
AluBI AGCT 2 cut(s) 121, 379
AluI AGCT 2 cut(s) 121, 379
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 208
ApoI RAATTY 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 318
AvaII GGWCC 1 cut(s) 318
BanII GRGCYC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 381
BccI CCATC 1 cut(s) 32
BceAI ACGGC 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 110
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 373
Bse1I ACTGG 1 cut(s) 266
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 79, 373
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 2 cut(s) 113, 285
BsiHKAI GWGCWC 1 cut(s) 381
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 79, 373
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 381
BspACI CCGC 2 cut(s) 191, 205
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 266
Bst4CI ACNGT 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 2 cut(s) 148, 218
BstF5I GGATG 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 358
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 318
CviAII CATG 1 cut(s) 419
CviJI RGCY 5 cut(s) 72, 121, 310, 379, 389
CviKI_1 RGCY 5 cut(s) 72, 121, 310, 379, 389
DdeI CTNAG 2 cut(s) 148, 218
Ecl136II GAGCTC 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 379
EcoICRI GAGCTC 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 381
FaeI CATG 1 cut(s) 422
FaiI YATR 9 cut(s) 54, 90, 102, 165, 233, 304, 315, 344, 420
FaqI GGGAC 1 cut(s) 331
FatI CATG 1 cut(s) 418
FauI CCCGC 1 cut(s) 184
FokI GGATG 1 cut(s) 345
FriOI GRGCYC 1 cut(s) 381
Hin1II CATG 1 cut(s) 422
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HinfI GANTC 2 cut(s) 26, 136
Hpy166II GTNNAC 2 cut(s) 197, 354
Hpy188I TCNGA 1 cut(s) 176
Hpy8I GTNNAC 2 cut(s) 197, 354
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 1 cut(s) 349
HpyF3I CTNAG 2 cut(s) 148, 218
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 1 cut(s) 422
LmnI GCTCC 2 cut(s) 126, 376
LpnPI CCDG 5 cut(s) 138, 247, 251, 292, 414
LweI GCATC 1 cut(s) 178
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 23
MboII GAAGA 3 cut(s) 265, 347, 416
MfeI CAATTG 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 4 cut(s) 59, 104, 408, 428
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 4 cut(s) 39, 134, 263, 289
MseI TTAA 1 cut(s) 62
MunI CAATTG 1 cut(s) 428
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 23
PaqCI CACCTGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 373
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
Psp124BI GAGCTC 1 cut(s) 381
Psp1406I AACGTT 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 318
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 62
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 1 cut(s) 20
SduI GDGCHC 1 cut(s) 381
SetI ASST 6 cut(s) 50, 123, 157, 300, 373, 381
SfaNI GCATC 1 cut(s) 178
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 4 cut(s) 59, 104, 408, 428
SsiI CCGC 2 cut(s) 191, 205
SstI GAGCTC 1 cut(s) 381
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 29
TasI AATT 4 cut(s) 59, 104, 408, 428
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseFI GTSAC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 3 cut(s) 117, 266, 348
Van91I CCANNNNNTGG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.