RLG00000003315

RAI1 like PD-(D/E)XK nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0022543
Physical Location & Seq
Forward (+)
47019696 .. 47021274
1579 bp
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UTR
Exon/CDS
Intron
RLM00000003315

Sequence Viewer

Length: 432 bp
ATGGGCGCTGAAATGGATTGCTGCGATTCAACTGATGATGGAAGGAGATTTTACGTGGAGTTAAAGACAAGTCGTGAGTTGGATTACCATACTGAGGAAAGATATGAGAGAGTGAAGCTCCTCAAATTTTGGATTCAATCATTCTTAGCAGGCGTTCCCTATATTGTAATTGGTTTTAGGGATGATTCAGGTCGATTTGTCCGCACAGAGCAACTAAGAACCAAAGATATAACACAGAGAGTGAAAATGAAGAACTACTGGCAGGGAGGAGTTTGCTTGGCATTTGCTGATGAGGTATTATGCTGGCTGTATGGAACAGTTAAAGAGAATGAAGATTACATATTGCAGTTTGCTCGTCCTTTCCAGCGTTTGGAGCTTCTACAAGCGCAATCTTGCCCAGAGGCAATCACTAACCACGTTGAGCGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

144

Amino Acids

16.95

Weight (kDa)

5.47

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 1 - 130 2.4e-38 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022543)

Species Orthologous Gene IDs
pyrus_communis pycom06g03860
rosa_laevigata RLG00000003315
rosa_multiflora Rmu_sc0003652.1_g000018
rosa_samantha Rh7AG228600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 424
AciI CCGC 2 cut(s) 202, 424
AcsI RAATTY 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 94, 370
AgsI TTSAA 2 cut(s) 30, 137
AluBI AGCT 2 cut(s) 118, 376
AluI AGCT 2 cut(s) 118, 376
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 2 cut(s) 8, 388
BbvI GCAGC 1 cut(s) 8
BccI CCATC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BsaAI YACGTR 1 cut(s) 55
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 370
Bse1I ACTGG 1 cut(s) 263
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 2 cut(s) 94, 370
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 2 cut(s) 110, 282
BseXI GCAGC 1 cut(s) 8
BslI CCNNNNNNNGG 2 cut(s) 94, 370
BspACI CCGC 2 cut(s) 202, 424
BspCNI CTCAG 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 424
BsrI ACTGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 319
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 151, 305
BstDEI CTNAG 3 cut(s) 93, 145, 215
BstF5I GGATG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 2 cut(s) 8, 388
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 8
BtsCI GGATG 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 151, 305
CfoI GCGC 2 cut(s) 8, 388
CviJI RGCY 3 cut(s) 118, 307, 376
CviKI_1 RGCY 3 cut(s) 118, 307, 376
DdeI CTNAG 3 cut(s) 93, 145, 215
FaiI YATR 7 cut(s) 90, 105, 162, 230, 301, 312, 341
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 1 cut(s) 22
GlaI GCGC 2 cut(s) 7, 387
GluI GCNGC 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 9
HhaI GCGC 2 cut(s) 8, 388
Hin6I GCGC 2 cut(s) 6, 386
HinP1I GCGC 2 cut(s) 6, 386
HinfI GANTC 3 cut(s) 26, 133, 185
Hpy188III TCNNGA 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 54, 417
HpyCH4V TGCA 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 3 cut(s) 93, 145, 215
HpySE526I ACGT 2 cut(s) 54, 417
HspAI GCGC 2 cut(s) 6, 386
LmnI GCTCC 2 cut(s) 123, 373
LpnPI CCDG 7 cut(s) 135, 174, 244, 248, 289, 377, 411
Lsp1109I GCAGC 1 cut(s) 8
MaeII ACGT 2 cut(s) 54, 417
MbiI CCGCTC 1 cut(s) 424
MboII GAAGA 2 cut(s) 262, 344
MluCI AATT 2 cut(s) 125, 168
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 5 cut(s) 88, 131, 260, 286, 394
MseI TTAA 2 cut(s) 62, 321
MwoI GCNNNNNNNGC 1 cut(s) 373
PfeI GAWTC 3 cut(s) 26, 133, 185
PflMI CCANNNNNTGG 1 cut(s) 370
PkrI GCNGC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 55
SaqAI TTAA 2 cut(s) 62, 321
SatI GCNGC 1 cut(s) 22
SetI ASST 6 cut(s) 57, 120, 193, 297, 378, 420
Sse9I AATT 2 cut(s) 125, 168
SsiI CCGC 2 cut(s) 202, 424
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 2 cut(s) 57, 420
TaqI TCGA 1 cut(s) 193
TasI AATT 2 cut(s) 125, 168
TfiI GAWTC 3 cut(s) 26, 133, 185
Tru1I TTAA 2 cut(s) 62, 321
Tru9I TTAA 2 cut(s) 62, 321
TseI GCWGC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 263, 345
Van91I CCANNNNNTGG 1 cut(s) 370
XapI RAATTY 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.