pycom06g19790
TCP Family

T-complex protein 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0029305
Physical Location & Seq
Reverse (-)
23095484 .. 23095960
477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19790.1

Sequence Viewer

Length: 477 bp
ATGGGTCGGACCACCACTGTGCTGGTTCGCGGGTCAAACCAGCTGGTGCTAGATGAGGCGGAGCGGAGCCTGCACGATGCTCTGTGTGTGGTGAGGTGTTTGGTGAGCAAAAGGTTTTTGATTGCAGGTGGCGGTGCACCCGAGATTGAGCTGTCGAGGCAGTTGGGGGCGTGGGCTAAGGTGCTGCAGGGGATGGAGGGTTATTGTGTGAAGTCGTTTGCTGAGGCGCTCGAGGTTGTTCCCTATACGCTGGCTGAGAATGCCGGGTTGAACCCAATTTCAATTGTGACTGAGCTGAGGAACCGGCACGCACAGGGAGAGATCGACACCGGGATTAATGTGAGGAAGGGGCAGATTACCAACATCTTGGAGGAGAATGTGGTGCAGCCATTGCTCGTCAGCACAAGTGCCATCACTCTGGCCGCCGAGTGTGTGCGGATGATTTTGAAGATCGATGACATAGTTACAGTAAGATAG

Protein Analysis

159

Amino Acids

17.15

Weight (kDa)

5.72

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 3 - 156 7.2e-55 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029305)

Species Orthologous Gene IDs
pyrus_communis pycom06g19790
rosa_multiflora Rmu_co8228149.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 64
AccII CGCG 1 cut(s) 30
AciI CCGC 6 cut(s) 30, 59, 64, 132, 423, 436
AcoI YGGCCR 1 cut(s) 420
AgsI TTSAA 3 cut(s) 271, 282, 448
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 3 cut(s) 43, 151, 295
AluI AGCT 3 cut(s) 43, 151, 295
Alw21I GWGCWC 1 cut(s) 139
Alw44I GTGCAC 1 cut(s) 135
Ama87I CYCGRG 2 cut(s) 140, 230
AoxI GGCC 1 cut(s) 420
ApaLI GTGCAC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 184, 385
AseI ATTAAT 1 cut(s) 336
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 9
AsuC2I CCSGG 2 cut(s) 265, 331
AsuHPI GGTGA 2 cut(s) 103, 115
AvaI CYCGRG 2 cut(s) 140, 230
AvaII GGWCC 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 139
BbvCI CCTCAGC 2 cut(s) 222, 296
BbvI GCAGC 2 cut(s) 171, 397
BccI CCATC 2 cut(s) 187, 419
BcnI CCSGG 2 cut(s) 265, 331
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 116
BisI GCNGC 3 cut(s) 185, 386, 423
BlsI GCNGC 3 cut(s) 186, 387, 424
Bme1390I CCNGG 2 cut(s) 265, 331
Bme18I GGWCC 1 cut(s) 9
BmeT110I CYCGRG 2 cut(s) 140, 230
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 2 cut(s) 68, 302
BmrFI CCNGG 2 cut(s) 265, 331
BmsI GCATC 1 cut(s) 67
Bpu10I CCTNAGC 3 cut(s) 177, 222, 296
BpuMI CCSGG 2 cut(s) 265, 331
Bsa29I ATCGAT 1 cut(s) 453
Bse118I RCCGGY 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 389
BseCI ATCGAT 1 cut(s) 453
BseGI GGATG 2 cut(s) 198, 444
BseMI GCAATG 1 cut(s) 389
BseMII CTCAG 4 cut(s) 213, 246, 282, 287
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 2 cut(s) 171, 397
BsgI GTGCAG 2 cut(s) 56, 404
Bsh1236I CGCG 1 cut(s) 30
BshFI GGCC 1 cut(s) 422
BshVI ATCGAT 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 139
BsiHKCI CYCGRG 2 cut(s) 140, 230
BsiSI CCGG 3 cut(s) 264, 304, 330
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 1 cut(s) 422
BsoBI CYCGRG 2 cut(s) 140, 230
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 2 cut(s) 321, 450
BspACI CCGC 6 cut(s) 30, 59, 64, 132, 423, 436
BspANI GGCC 1 cut(s) 422
BspCNI CTCAG 4 cut(s) 214, 247, 283, 288
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 68, 302
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 303
BssMI GATC 2 cut(s) 321, 450
Bst4CI ACNGT 2 cut(s) 19, 469
BstAPI GCANNNNNTGC 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 71, 252, 309
BstDEI CTNAG 5 cut(s) 177, 222, 255, 291, 296
BstF5I GGATG 2 cut(s) 198, 444
BstFNI CGCG 1 cut(s) 30
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 324, 453
BstMBI GATC 2 cut(s) 321, 450
BstMWI GCNNNNNNNGC 4 cut(s) 70, 157, 260, 391
BstSCI CCNGG 2 cut(s) 263, 329
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 139
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 171, 397
BstXI CCANNNNNNTGG 3 cut(s) 22, 367, 418
Bsu15I ATCGAT 1 cut(s) 453
BsuRI GGCC 1 cut(s) 422
BsuTUI ATCGAT 1 cut(s) 453
BtsCI GGATG 2 cut(s) 198, 444
BtsIMutI CAGTG 1 cut(s) 15
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 3 cut(s) 71, 252, 309
CfoI GCGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 453
CviJI RGCY 8 cut(s) 43, 69, 151, 176, 254, 295, 388, 422
CviKI_1 RGCY 8 cut(s) 43, 69, 151, 176, 254, 295, 388, 422
DdeI CTNAG 5 cut(s) 177, 222, 255, 291, 296
DpnI GATC 2 cut(s) 323, 452
DpnII GATC 2 cut(s) 321, 450
EaeI YGGCCR 1 cut(s) 420
EciI GGCGGA 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 9
Eco88I CYCGRG 2 cut(s) 140, 230
FaiI YATR 2 cut(s) 246, 461
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 3 cut(s) 185, 386, 423
FokI GGATG 2 cut(s) 205, 451
Fsp4HI GCNGC 3 cut(s) 185, 386, 423
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 228
GluI GCNGC 3 cut(s) 185, 386, 423
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 1 cut(s) 422
HapII CCGG 3 cut(s) 264, 304, 330
HhaI GCGC 1 cut(s) 229
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HpaII CCGG 3 cut(s) 264, 304, 330
HphI GGTGA 2 cut(s) 103, 115
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 19, 469
HpyCH4V TGCA 5 cut(s) 73, 125, 137, 187, 385
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 157, 260, 391
HpyF3I CTNAG 5 cut(s) 177, 222, 255, 291, 296
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 2 cut(s) 321, 450
LmnI GCTCC 2 cut(s) 61, 66
Lsp1109I GCAGC 2 cut(s) 171, 397
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 286, 463
MalI GATC 2 cut(s) 323, 452
MbiI CCGCTC 1 cut(s) 64
MboI GATC 2 cut(s) 321, 450
MboII GAAGA 1 cut(s) 460
MfeI CAATTG 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 2 cut(s) 276, 282
MnlI CCTC 9 cut(s) 49, 87, 150, 190, 217, 226, 291, 336, 364
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 3 cut(s) 264, 304, 330
MspR9I CCNGG 2 cut(s) 265, 331
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 265
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 70, 157, 260, 391
NciI CCSGG 2 cut(s) 265, 331
NdeII GATC 2 cut(s) 321, 450
NlaIV GGNNCC 2 cut(s) 68, 302
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 286
OliI CACNNNNGTG 1 cut(s) 17
PaeR7I CTCGAG 1 cut(s) 230
PaqCI CACCTGC 1 cut(s) 116
PctI GAATGC 1 cut(s) 265
PkrI GCNGC 3 cut(s) 186, 387, 424
PshBI ATTAAT 1 cut(s) 336
PspN4I GGNNCC 2 cut(s) 68, 302
PspPI GGNCC 1 cut(s) 9
PspXI VCTCGAGB 1 cut(s) 230
PstI CTGCAG 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 3 cut(s) 185, 386, 423
Sau3AI GATC 2 cut(s) 321, 450
Sau96I GGNCC 1 cut(s) 9
ScrFI CCNGG 2 cut(s) 265, 331
SduI GDGCHC 1 cut(s) 139
SetI ASST 8 cut(s) 45, 98, 116, 130, 153, 183, 237, 297
SfaNI GCATC 1 cut(s) 67
SfcI CTRYAG 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 230
SinI GGWCC 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 2 cut(s) 276, 282
SsiI CCGC 6 cut(s) 30, 59, 64, 132, 423, 436
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 263, 329
TaaI ACNGT 2 cut(s) 19, 469
TaqI TCGA 4 cut(s) 155, 231, 324, 453
TasI AATT 2 cut(s) 276, 282
TauI GCSGC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 2 cut(s) 184, 385
Tsp45I GTSAC 1 cut(s) 286
TspRI CASTG 1 cut(s) 22
VneI GTGCAC 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 9
VspI ATTAAT 1 cut(s) 336
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.