pycom06g20020

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0022317
Physical Location & Seq
Reverse (-)
23218259 .. 23222001
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGTCTGAAGTCATATATTGCTCCCGCTGGCCTGGTCCCGCGATTCCCTTCAAGCTCATTTGCAACCTAGCAAACCGCTATGAAACGAGGGAAGGGGAATTCTTCCACACGGCGCTGGTTTGGCTCCACAACAACCACCCCAAAACCCTAGCCTGCAACATCGAGGCTATTGCAGGCGGCAAACGTTCCCAGGTTTCTTTTGGCAGTTTAAAGGACTTTGTCATTATTCTGTACCGGCTTCTTCTTCCAGAAGGAGGCAGCCACAATAGTGTCATTGATGTTGCCATCCACAAGAAGGGTGTTCTTGAAAGGTACGAGCGTGACCCCAATTATCAGTTTTTACACAATCGTGTTTCAGATGTTTTTGCGGCCGACTTTAGGTCTGATTTTGAAAAGTTGAAGAAAAAGAAGCCCAATGAGAAAATGAATTGTGATGATTTTAGCAAGTCAAGTGCGCCCTTTGTCCTTGGACCCATAGAAAACGGCTGCACGTCCTACGATTACAATGCCAGCTGTATTGCTATCACTACTATTCCACTGTGTGAAAGCATCGCAAGGAAGGTTTTTCCTCGCGGTGAGGAAGAGGGCGAGAACGAGGTTGATTACACATATAGAGTCCTGAATCGGCTAAGTAAAGAGGTTTTGGAGCCAGTGTGGGAGCATAGTCCATATTTTTTGGTGGATAACGTGAAAGCCGGCAAGTCCAATACTTCGGCAGATGTTCTCCTTCCCCATAAGATCATCTTCTATGAACTTCAATGGAAGGCAATGGTGGAGGACTTGTCTAACAAGGGCAAGTTGAAGAATTGCTTGGCTGTATGTGAAGTCACGGGAACCTTGAGTTGGGTGTCGGTGGCTATGTGGCTATTGGTGTCTCAACTGAGTGATCACAAGCCCTGGAAAGGAAAGGTCATCCCTTTCACTCGGAATCCCAAACGGCTCCATCTCATACAAGGCGATGATCTCAAGTCCAAGCTAGCCTGCTTCAGAGGCCCAGACTTCAGCGGAAATAGCGCGACAACTCAAAAGCCGGACTTCATCTTCAAGAGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

352

Amino Acids

39.92

Weight (kDa)

8.67

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 13 - 81 9.1e-11 Domain of unknown function (DUF2828)
DUF2828 PF11443 97 - 219 2.6e-11 Domain of unknown function (DUF2828)
DUF7788 PF25043 269 - 334 7e-07 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022317)

Species Orthologous Gene IDs
malus_domestica MD00G1160800.v1.1 MD00G1160900.v1.1 MD06G1229000.v1.1
pyrus_communis pycom06g20020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 41, 573, 1016
AciI CCGC 7 cut(s) 25, 39, 76, 177, 368, 573, 1005
AclI AACGTT 1 cut(s) 184
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 3 cut(s) 27, 970, 985
AdeI CACNNNGTG 1 cut(s) 542
AfaI GTAC 2 cut(s) 233, 314
AfiI CCNNNNNNNGG 5 cut(s) 254, 295, 378, 843, 902
AgsI TTSAA 7 cut(s) 52, 308, 392, 400, 758, 802, 1045
AhdI GACNNNNNGTC 1 cut(s) 379
AjiI CACGTC 1 cut(s) 492
AjnI CCWGG 3 cut(s) 31, 189, 896
AjuI GAANNNNNNNTTGG 4 cut(s) 794, 826, 826, 858
AleI CACNNNNGTG 2 cut(s) 267, 348
AluBI AGCT 3 cut(s) 55, 513, 976
AluI AGCT 3 cut(s) 55, 513, 976
Alw26I GTCTC 1 cut(s) 879
AoxI GGCC 3 cut(s) 29, 369, 991
ApeKI GCWGC 2 cut(s) 258, 486
ApoI RAATTY 1 cut(s) 98
AspLEI GCGC 3 cut(s) 115, 457, 1016
AspS9I GGNCC 3 cut(s) 35, 470, 992
AsuHPI GGTGA 1 cut(s) 587
AsuNHI GCTAGC 1 cut(s) 976
AvaII GGWCC 2 cut(s) 35, 470
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BbvI GCAGC 2 cut(s) 270, 473
BccI CCATC 2 cut(s) 293, 951
BceAI ACGGC 3 cut(s) 126, 499, 953
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 488, 522
BciT130I CCWGG 3 cut(s) 33, 191, 898
BclI TGATCA 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 879
BfaI CTAG 3 cut(s) 68, 149, 977
BfoI RGCGCY 1 cut(s) 116
BisI GCNGC 4 cut(s) 178, 259, 369, 487
BlsI GCNGC 4 cut(s) 179, 260, 370, 488
Bme1390I CCNGG 3 cut(s) 33, 191, 898
Bme18I GGWCC 2 cut(s) 35, 470
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgBI CACGTC 1 cut(s) 492
BmgT120I GGNCC 3 cut(s) 35, 470, 992
BmiI GGNNCC 6 cut(s) 37, 125, 472, 648, 835, 941
BmrFI CCNGG 3 cut(s) 33, 191, 898
BmsI GCATC 1 cut(s) 558
BmtI GCTAGC 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 859, 950
BsaJI CCNNGG 3 cut(s) 189, 466, 896
BsaXI ACNNNNNCTCC 4 cut(s) 638, 668, 767, 797
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 295, 378, 843, 902
Bse118I RCCGGY 2 cut(s) 234, 695
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 3 cut(s) 33, 191, 898
BseDI CCNNGG 3 cut(s) 189, 466, 896
BseGI GGATG 2 cut(s) 285, 912
BseLI CCNNNNNNNGG 5 cut(s) 254, 295, 378, 843, 902
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 1 cut(s) 872
BseNI ACTGG 1 cut(s) 650
BseX3I CGGCCG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 270, 473
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 3 cut(s) 41, 573, 1016
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 3 cut(s) 31, 371, 993
BsiEI CGRYCG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 235, 696, 1031
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 5 cut(s) 254, 295, 378, 843, 902
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 3 cut(s) 31, 371, 993
Bsp143I GATC 3 cut(s) 738, 886, 961
BspACI CCGC 7 cut(s) 25, 39, 76, 177, 368, 573, 1005
BspANI GGCC 3 cut(s) 31, 371, 993
BspCNI CTCAG 1 cut(s) 873
BspFNI CGCG 3 cut(s) 41, 573, 1016
BspLI GGNNCC 6 cut(s) 37, 125, 472, 648, 835, 941
BspOI GCTAGC 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 2 cut(s) 234, 695
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 2 cut(s) 234, 695
BssECI CCNNGG 3 cut(s) 189, 466, 896
BssMI GATC 3 cut(s) 738, 886, 961
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 3 cut(s) 33, 191, 898
Bst4CI ACNGT 1 cut(s) 540
Bst6I CTCTTC 1 cut(s) 576
BstC8I GCNNGC 7 cut(s) 29, 154, 175, 511, 697, 978, 982
BstDEI CTNAG 2 cut(s) 629, 881
BstF5I GGATG 2 cut(s) 285, 912
BstFNI CGCG 3 cut(s) 41, 573, 1016
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 3 cut(s) 115, 457, 1016
BstKTI GATC 3 cut(s) 741, 889, 964
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 3 cut(s) 738, 886, 961
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 3 cut(s) 121, 990, 1011
BstNI CCWGG 3 cut(s) 33, 191, 898
BstSCI CCNGG 3 cut(s) 31, 189, 896
BstUI CGCG 3 cut(s) 41, 573, 1016
BstV1I GCAGC 2 cut(s) 270, 473
BstZI CGGCCG 1 cut(s) 369
BsuRI GGCC 3 cut(s) 31, 371, 993
BtgZI GCGATG 2 cut(s) 535, 972
BtrI CACGTC 1 cut(s) 492
BtsCI GGATG 2 cut(s) 285, 912
BtsIMutI CAGTG 2 cut(s) 536, 657
Cac8I GCNNGC 7 cut(s) 29, 154, 175, 511, 697, 978, 982
CfoI GCGC 3 cut(s) 115, 457, 1016
Cfr10I RCCGGY 2 cut(s) 234, 695
Cfr13I GGNCC 3 cut(s) 35, 470, 992
Csp6I GTAC 2 cut(s) 232, 313
CviQI GTAC 2 cut(s) 232, 313
DdeI CTNAG 2 cut(s) 629, 881
DpnI GATC 3 cut(s) 740, 888, 963
DpnII GATC 3 cut(s) 738, 886, 961
DraI TTTAAA 1 cut(s) 210
DraIII CACNNNGTG 1 cut(s) 542
DriI GACNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 369
EagI CGGCCG 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 576
Eam1105I GACNNNNNGTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 576
EclXI CGGCCG 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 35, 470
Eco52I CGGCCG 1 cut(s) 369
Eco57I CTGAAG 3 cut(s) 27, 970, 985
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 31, 189, 896
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
FalI AAGNNNNNCTT 6 cut(s) 728, 760, 794, 826, 1019, 1051
FaqI GGGAC 1 cut(s) 21
FauI CCCGC 2 cut(s) 32, 46
FbaI TGATCA 1 cut(s) 886
Fnu4HI GCNGC 4 cut(s) 178, 259, 369, 487
FokI GGATG 2 cut(s) 272, 899
Fsp4HI GCNGC 4 cut(s) 178, 259, 369, 487
FspBI CTAG 3 cut(s) 68, 149, 977
GlaI GCGC 3 cut(s) 114, 456, 1015
GluI GCNGC 4 cut(s) 178, 259, 369, 487
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 3 cut(s) 31, 371, 993
HapII CCGG 3 cut(s) 235, 696, 1031
HhaI GCGC 3 cut(s) 115, 457, 1016
Hin6I GCGC 3 cut(s) 113, 455, 1014
HinP1I GCGC 3 cut(s) 113, 455, 1014
HinfI GANTC 4 cut(s) 43, 615, 622, 928
HpaII CCGG 3 cut(s) 235, 696, 1031
HphI GGTGA 1 cut(s) 587
Hpy188I TCNGA 5 cut(s) 7, 358, 385, 927, 989
Hpy188III TCNNGA 4 cut(s) 248, 305, 619, 1045
HpyAV CCTTC 7 cut(s) 58, 86, 245, 289, 553, 737, 757
HpyCH4III ACNGT 1 cut(s) 540
HpyCH4IV ACGT 3 cut(s) 184, 491, 687
HpyCH4V TGCA 4 cut(s) 63, 156, 173, 489
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 990, 1011
HpyF3I CTNAG 2 cut(s) 629, 881
HpySE526I ACGT 3 cut(s) 184, 491, 687
HspAI GCGC 3 cut(s) 113, 455, 1014
KroI GCCGGC 1 cut(s) 695
KroNI GCCGGC 1 cut(s) 697
Ksp22I TGATCA 1 cut(s) 886
Kzo9I GATC 3 cut(s) 738, 886, 961
LmnI GCTCC 5 cut(s) 26, 129, 646, 658, 945
Lsp1109I GCAGC 2 cut(s) 270, 473
LweI GCATC 1 cut(s) 558
MaeI CTAG 3 cut(s) 68, 149, 977
MaeII ACGT 3 cut(s) 184, 491, 687
MaeIII GTNAC 2 cut(s) 320, 826
MalI GATC 3 cut(s) 740, 888, 963
MboI GATC 3 cut(s) 738, 886, 961
MboII GAAGA 8 cut(s) 94, 233, 236, 412, 593, 736, 814, 1033
MluCI AATT 4 cut(s) 98, 328, 427, 805
MlyI GAGTC 1 cut(s) 624
MroNI GCCGGC 1 cut(s) 695
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 267, 348
MspA1I CMGCKG 3 cut(s) 27, 513, 1005
MspI CCGG 3 cut(s) 235, 696, 1031
MspR9I CCNGG 3 cut(s) 33, 191, 898
MvaI CCWGG 3 cut(s) 33, 191, 898
MvnI CGCG 3 cut(s) 41, 573, 1016
MwoI GCNNNNNNNGC 3 cut(s) 121, 990, 1011
NaeI GCCGGC 1 cut(s) 697
NdeII GATC 3 cut(s) 738, 886, 961
NgoMIV GCCGGC 1 cut(s) 695
NheI GCTAGC 1 cut(s) 976
NlaIV GGNNCC 6 cut(s) 37, 125, 472, 648, 835, 941
NmuCI GTSAC 2 cut(s) 320, 826
OliI CACNNNNGTG 2 cut(s) 267, 348
PdiI GCCGGC 1 cut(s) 697
PfeI GAWTC 3 cut(s) 43, 622, 928
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 4 cut(s) 179, 260, 370, 488
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Psp1406I AACGTT 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 31, 189, 896
PspGI CCWGG 3 cut(s) 31, 189, 896
PspN4I GGNNCC 6 cut(s) 37, 125, 472, 648, 835, 941
PspPI GGNCC 3 cut(s) 35, 470, 992
PsyI GACNNNGTC 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 513
RsaI GTAC 2 cut(s) 233, 314
RsaNI GTAC 2 cut(s) 232, 313
RseI CAYNNNNRTG 2 cut(s) 267, 348
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 4 cut(s) 178, 259, 369, 487
Sau3AI GATC 3 cut(s) 738, 886, 961
Sau96I GGNCC 3 cut(s) 35, 470, 992
SchI GAGTC 1 cut(s) 624
ScrFI CCNGG 3 cut(s) 33, 191, 898
SfaNI GCATC 1 cut(s) 558
SinI GGWCC 2 cut(s) 35, 470
SmiMI CAYNNNNRTG 2 cut(s) 267, 348
SmlI CTYRAG 2 cut(s) 838, 965
SmoI CTYRAG 2 cut(s) 838, 965
Sse9I AATT 4 cut(s) 98, 328, 427, 805
SsiI CCGC 7 cut(s) 25, 39, 76, 177, 368, 573, 1005
SspMI CTAG 3 cut(s) 68, 149, 977
StyD4I CCNGG 3 cut(s) 31, 189, 896
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 540
TaiI ACGT 3 cut(s) 187, 494, 690
TaqI TCGA 1 cut(s) 162
TasI AATT 4 cut(s) 98, 328, 427, 805
TauI GCSGC 2 cut(s) 180, 371
TfiI GAWTC 3 cut(s) 43, 622, 928
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 2 cut(s) 543, 657
TseFI GTSAC 2 cut(s) 320, 826
TseI GCWGC 2 cut(s) 258, 486
Tsp45I GTSAC 2 cut(s) 320, 826
TspDTI ATGAA 4 cut(s) 96, 440, 765, 1027
TspRI CASTG 2 cut(s) 543, 657
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 35, 470
XapI RAATTY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XspI CTAG 3 cut(s) 68, 149, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.