pycom07g02770

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0022522
Physical Location & Seq
Forward (+)
2338471 .. 2342301
3831 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02770.1

Sequence Viewer

Length: 2088 bp
ATGGAGAAGAAGCAACTACGGGAGCACTGGGGGCAGAAGTTGATCGATCTTATGGTGGTAGATGAATTAGCAGATTCGTGCTTGTCGCCAGAACTTGACTCTGGTCTAGATAGGCCCGTCTTTGAAGATATTATTGAATATGAGGACAAATCAGAAACTCCTCCTGGAAAGTCAAGTTGGGAAAATGATTCCTCTCTATGGGGGACAAATAGTACAAGAGAAGATGCCACATCATCTTGGGGGAAAACTCCAGCTAGAGAAGCTACCACATCTACATGGGGGATAGATAAGGCAGCAGAAGATACCACTCCTGCATGGGGGACAAATACTGCCAGGGAAGATGCGACATCTGCCTGGGGGAAAACTAGAGAGCATATCACTTCTGCAGGGGGCGTAGATAAGACAAGTGAAAATGATACATCATCGTGGGGTAAAACTCTAGCTAAAGAACCTGCCGCATCTTCTTGGGGGATAGCCAAGGCAGCAGAAGATACCACTCCAGCATGGGGGACAAATACTGCCAGAGAAGATGCCGCGAAAGCCGGAGAACCTCCCACTTCTGCCTGGGGGGCAAGTAAGGTACGTGAAAATGATACACCATCTTGGGGGAAAACTCCAGCTAAAGAAACTTCGACATCCACTTGGGGGATAGATAAGGCAGCAGAAGATACCACTCCTGTATGGGGGACAAGTACTGCCAGAGAAGATGCCACACCTGCCTGGGGGAAATCTAGAGAAAAGACTGCTGCTGCCTGGGGAACAGATAAGGAAAGTGATAATGATACATCATCTTGGGGGAAAAATCCAGCTAGAAAATCTACCACCTCTACTTGGGGGATAGATAAGGCAGCAGAAGATACCACTTCTGCATGGGGAACAAGTACTGCCAGAGAAGATGCCACATCAGCTTGGGGGAAATCTAGAGAAAATACTGCTTCTGCCTGGGGAACAGATAAGGAAAGTGAAAATGATAGATCATCTTGGGGGAAAACTCCAGCTAGAAAACCTACCAACTCTACTTGGGGGACAGAGAAGGCAGCAGAAGATACCACTCCTACATCGGGGACAAATACTGCCAGAGAAGATGCCACATCTACCTGGGGGAAAACTAGAGAACATACCACTTCTACCTGGGGGACAGATAAGGCAAGTGAAAATGATTGGAGTGGTAGAGGGGCAGAGAAAGTCGCACCAATAGACCTCCCGCTGACAACAAAAGACGTAGATGAAAAGGACAATGGATGGGGTGCTGTTTCTTCTGGGGAAACAGAAACAGGGAATGAAAATGCTTGGGAACAGAATAAGTCTTCCGAAATTTCAACTGGTTCTTGGGGGCAGAAAAATCCTCCCGAGACAGAAACTCAACCTGCAAGCTTGTCTGGGTGGGATTCACCTAGTGGAGATGGTAATACTGGTGAAAGACATCACCAATGGGGACAAAACAAGAAAAGTCGTTTTGAAGGTTCAAAGAGTTGGGTTTCTAGTCCCGGGGAGTGGAAGAATAAGAACCGTCCTGCTAAACCACCTGGTATGATGAATGATAATTCCAGTGTGGTTGGACTATATACTGCAACAAGACAAAGATTGGATATGTTCCCTAGCGAGGAGCAAGATGTTCTTTCTAATATTGAACCTATTATGCGCTCTATCCGAAGAATAATGCATCAATCTGGGTACAATGATGGGGATCCACTGTCAGCTGATGACCAGTCATTTATAATTGATAATGTTCTCAATTACCACCCGGATAAAGCTGTTAAAATGGGTTCTGGAATTGGCCATCTCACAGTTGATAGGCACGGCAGTTTCCAGGACAGCAGATGCTTTTTTGTTGTGACAACAGATGGTCACAAGGAAGATTTTTCGTATAGAAAATGCCTGGAGAATTACATTAAGGAAAAGTATGCTGATGTTGCCGAAACATTCATAGGCAAGTATTTTACCAGGAGAGGAGGGAATCGGGAGCGAAACCCCACTCCAAGTCAATACCCCATTCCACAACAGACTCCAAGCCAAACTCCCATTGCAGAGGAAACTGTAAGTCAAACCCCCATTTCGGAGCAAACTGGGAACGAGGAGACGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

696

Amino Acids

76.05

Weight (kDa)

4.91

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 552 - 627 3.7e-28 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022522)

Species Orthologous Gene IDs
pyrus_communis pycom01g13750 pycom02g25690 pycom07g02770 pycom13g22730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1718
AarI CACCTGC 1 cut(s) 724
Acc36I ACCTGC 3 cut(s) 460, 724, 1375
AccII CGCG 1 cut(s) 536
AciI CCGC 3 cut(s) 456, 534, 1205
AclWI GGATC 2 cut(s) 1682, 1695
AcoI YGGCCR 1 cut(s) 1777
AcsI RAATTY 1 cut(s) 1314
AdeI CACNNNGTG 1 cut(s) 1397
AfaI GTAC 5 cut(s) 214, 582, 694, 883, 1676
AgsI TTSAA 6 cut(s) 125, 137, 1320, 1460, 1467, 1631
AjuI GAANNNNNNNTTGG 2 cut(s) 160, 192
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 1346, 2072
AlwI GGATC 2 cut(s) 1682, 1695
Ama87I CYCGRG 2 cut(s) 1349, 1487
AoxI GGCC 2 cut(s) 113, 1777
ApeKI GCWGC 7 cut(s) 293, 482, 659, 746, 749, 848, 1037
ApoI RAATTY 1 cut(s) 1314
AspLEI GCGC 1 cut(s) 1644
AspS9I GGNCC 1 cut(s) 114
AsuC2I CCSGG 3 cut(s) 1488, 1489, 1745
AsuHPI GGTGA 3 cut(s) 1383, 1418, 1427
AvaI CYCGRG 2 cut(s) 1349, 1487
BalI TGGCCA 1 cut(s) 1779
BamHI GGATCC 1 cut(s) 1687
BbsI GAAGAC 1 cut(s) 1299
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 7 cut(s) 305, 494, 671, 733, 736, 860, 1049
BccI CCATC 6 cut(s) 607, 1236, 1397, 1676, 1788, 1838
BceAI ACGGC 1 cut(s) 1816
BcnI CCSGG 3 cut(s) 1488, 1489, 1745
BcoDI GTCTC 2 cut(s) 1346, 2072
BfmI CTRYAG 1 cut(s) 384
BfuAI ACCTGC 3 cut(s) 460, 724, 1375
BglI GCCNNNNNGGC 1 cut(s) 569
BisI GCNGC 9 cut(s) 294, 456, 483, 534, 660, 747, 750, 849, 1038
BlsI GCNGC 9 cut(s) 295, 457, 484, 535, 661, 748, 751, 850, 1039
BmcAI AGTACT 2 cut(s) 694, 883
BmeT110I CYCGRG 2 cut(s) 1349, 1487
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 1689
BmrI ACTGGG 2 cut(s) 37, 2076
BmsI GCATC 9 cut(s) 214, 331, 467, 520, 697, 886, 1075, 1672, 1811
BmuI ACTGGG 2 cut(s) 37, 2076
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 1299
BpmI CTGGAG 5 cut(s) 234, 483, 600, 978, 1901
BpuMI CCSGG 3 cut(s) 1488, 1489, 1745
Bsa29I ATCGAT 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 584
BsaBI GATNNNNATC 1 cut(s) 1686
BsaXI ACNNNNNCTCC 1 cut(s) 2069
Bse1I ACTGG 6 cut(s) 32, 1327, 1417, 1548, 1708, 2071
Bse3DI GCAATG 1 cut(s) 2022
Bse8I GATNNNNATC 1 cut(s) 1686
BseCI ATCGAT 1 cut(s) 45
BseGI GGATG 2 cut(s) 635, 1247
BseJI GATNNNNATC 1 cut(s) 1686
BseMI GCAATG 1 cut(s) 2022
BseNI ACTGG 6 cut(s) 32, 1327, 1417, 1548, 1708, 2071
BseRI GAGGAG 3 cut(s) 150, 1619, 1965
BseXI GCAGC 7 cut(s) 305, 494, 671, 733, 736, 860, 1049
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 2 cut(s) 115, 1779
BshVI ATCGAT 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 1349, 1487
BsiSI CCGG 3 cut(s) 543, 1488, 1745
BslFI GGGAC 9 cut(s) 217, 334, 523, 700, 1039, 1078, 1150, 1449, 1470
BsmAI GTCTC 2 cut(s) 1346, 2072
BsmBI CGTCTC 1 cut(s) 2072
BsmFI GGGAC 9 cut(s) 217, 334, 523, 700, 1039, 1078, 1150, 1449, 1470
BsnI GGCC 2 cut(s) 115, 1779
BsoBI CYCGRG 2 cut(s) 1349, 1487
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 4 cut(s) 42, 46, 974, 1687
BspACI CCGC 3 cut(s) 456, 534, 1205
BspANI GGCC 2 cut(s) 115, 1779
BspDI ATCGAT 1 cut(s) 45
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 1689
BspMAI CTGCAG 1 cut(s) 388
BspMI ACCTGC 3 cut(s) 460, 724, 1375
BspPI GGATC 2 cut(s) 1682, 1695
BsrDI GCAATG 1 cut(s) 2022
BsrI ACTGG 6 cut(s) 32, 1327, 1417, 1548, 1708, 2071
BssMI GATC 4 cut(s) 42, 46, 974, 1687
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 4 cut(s) 1511, 1695, 1789, 2038
BstBAI YACGTR 1 cut(s) 584
BstC8I GCNNGC 1 cut(s) 1372
BstF5I GGATG 2 cut(s) 635, 1247
BstFNI CGCG 1 cut(s) 536
BstHHI GCGC 1 cut(s) 1644
BstKTI GATC 4 cut(s) 45, 49, 977, 1690
BstMAI GTCTC 2 cut(s) 1346, 2072
BstMBI GATC 4 cut(s) 42, 46, 974, 1687
BstMWI GCNNNNNNNGC 9 cut(s) 31, 260, 350, 482, 539, 569, 716, 905, 1913
BstPAI GACNNNNGTC 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 7 cut(s) 305, 494, 671, 733, 736, 860, 1049
BstV2I GAAGAC 1 cut(s) 1299
BstX2I RGATCY 1 cut(s) 1687
BstYI RGATCY 1 cut(s) 1687
Bsu15I ATCGAT 1 cut(s) 45
BsuRI GGCC 2 cut(s) 115, 1779
BsuTUI ATCGAT 1 cut(s) 45
BtsCI GGATG 2 cut(s) 635, 1247
BtsIMutI CAGTG 3 cut(s) 25, 1555, 1691
BveI ACCTGC 3 cut(s) 460, 724, 1375
Cac8I GCNNGC 1 cut(s) 1372
CfoI GCGC 1 cut(s) 1644
Cfr13I GGNCC 1 cut(s) 114
Cfr9I CCCGGG 1 cut(s) 1487
ClaI ATCGAT 1 cut(s) 45
CsiI ACCWGGT 1 cut(s) 1525
Csp6I GTAC 5 cut(s) 213, 581, 693, 882, 1675
CviAII CATG 4 cut(s) 276, 315, 504, 870
CviQI GTAC 5 cut(s) 213, 581, 693, 882, 1675
DpnI GATC 4 cut(s) 44, 48, 976, 1689
DpnII GATC 4 cut(s) 42, 46, 974, 1687
DraIII CACNNNGTG 1 cut(s) 1397
EaeI YGGCCR 1 cut(s) 1777
Eco130I CCWWGG 1 cut(s) 477
Eco88I CYCGRG 2 cut(s) 1349, 1487
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 1665
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 2072
FaeI CATG 4 cut(s) 279, 318, 507, 873
FalI AAGNNNNNCTT 2 cut(s) 1602, 1634
FaqI GGGAC 9 cut(s) 217, 334, 523, 700, 1039, 1078, 1150, 1449, 1470
FatI CATG 4 cut(s) 275, 314, 503, 869
FauI CCCGC 1 cut(s) 1212
Fnu4HI GCNGC 9 cut(s) 294, 456, 483, 534, 660, 747, 750, 849, 1038
FokI GGATG 2 cut(s) 622, 1254
Fsp4HI GCNGC 9 cut(s) 294, 456, 483, 534, 660, 747, 750, 849, 1038
GlaI GCGC 1 cut(s) 1643
GluI GCNGC 9 cut(s) 294, 456, 483, 534, 660, 747, 750, 849, 1038
GsuI CTGGAG 5 cut(s) 234, 483, 600, 978, 1901
HaeIII GGCC 2 cut(s) 115, 1779
HapII CCGG 3 cut(s) 543, 1488, 1745
HhaI GCGC 1 cut(s) 1644
Hin1II CATG 4 cut(s) 279, 318, 507, 873
Hin6I GCGC 1 cut(s) 1642
HinP1I GCGC 1 cut(s) 1642
HindIII AAGCTT 1 cut(s) 1372
HinfI GANTC 6 cut(s) 74, 98, 188, 1388, 1957, 2005
HpaII CCGG 3 cut(s) 543, 1488, 1745
HphI GGTGA 3 cut(s) 1383, 1418, 1427
Hpy188I TCNGA 4 cut(s) 154, 1312, 1652, 2059
Hpy188III TCNNGA 6 cut(s) 107, 732, 921, 1349, 1770, 1961
HpyAV CCTTC 2 cut(s) 1027, 1454
HpyCH4III ACNGT 4 cut(s) 1511, 1695, 1789, 2038
HpyCH4IV ACGT 2 cut(s) 583, 1221
HpyCH4V TGCA 7 cut(s) 314, 386, 869, 1370, 1571, 1663, 2027
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 260, 350, 482, 539, 569, 716, 905, 1913
HpySE526I ACGT 2 cut(s) 583, 1221
Hsp92II CATG 4 cut(s) 279, 318, 507, 873
HspAI GCGC 1 cut(s) 1642
Kzo9I GATC 4 cut(s) 42, 46, 974, 1687
LmnI GCTCC 4 cut(s) 22, 1606, 1963, 2059
Lsp1109I GCAGC 7 cut(s) 305, 494, 671, 733, 736, 860, 1049
LweI GCATC 9 cut(s) 214, 331, 467, 520, 697, 886, 1075, 1672, 1811
MabI ACCWGGT 1 cut(s) 1525
MaeII ACGT 2 cut(s) 583, 1221
MaeIII GTNAC 2 cut(s) 1834, 1847
MalI GATC 4 cut(s) 44, 48, 976, 1689
MboI GATC 4 cut(s) 42, 46, 974, 1687
MflI RGATCY 1 cut(s) 1687
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 1779
MluCI AATT 7 cut(s) 65, 1314, 1543, 1719, 1735, 1773, 1885
MluNI TGGCCA 1 cut(s) 1779
MlyI GAGTC 2 cut(s) 92, 1999
MmeI TCCRAC 1 cut(s) 1537
Mox20I TGGCCA 1 cut(s) 1779
Mph1103I ATGCAT 1 cut(s) 1665
MscI TGGCCA 1 cut(s) 1779
MseI TTAA 2 cut(s) 1758, 1893
MslI CAYNNNNRTG 2 cut(s) 274, 424
Msp20I TGGCCA 1 cut(s) 1779
MspA1I CMGCKG 2 cut(s) 1207, 1700
MspI CCGG 3 cut(s) 543, 1488, 1745
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 9 cut(s) 31, 260, 350, 482, 539, 569, 716, 905, 1913
NciI CCSGG 3 cut(s) 1488, 1489, 1745
NdeII GATC 4 cut(s) 42, 46, 974, 1687
NlaIII CATG 4 cut(s) 279, 318, 507, 873
NlaIV GGNNCC 1 cut(s) 1689
NmuCI GTSAC 2 cut(s) 1834, 1847
NsiI ATGCAT 1 cut(s) 1665
PaqCI CACCTGC 1 cut(s) 724
PfeI GAWTC 4 cut(s) 74, 188, 1388, 1957
PfoI TCCNGGA 2 cut(s) 163, 1809
PkrI GCNGC 9 cut(s) 295, 457, 484, 535, 661, 748, 751, 850, 1039
PleI GAGTC 2 cut(s) 92, 1999
PpsI GAGTC 2 cut(s) 92, 1999
Ppu21I YACGTR 1 cut(s) 584
PshAI GACNNNNGTC 1 cut(s) 102
PsiI TTATAA 1 cut(s) 1718
PspN4I GGNNCC 1 cut(s) 1689
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 1 cut(s) 1687
PvuII CAGCTG 1 cut(s) 1700
RsaI GTAC 5 cut(s) 214, 582, 694, 883, 1676
RsaNI GTAC 5 cut(s) 213, 581, 693, 882, 1675
RseI CAYNNNNRTG 2 cut(s) 274, 424
SaqAI TTAA 2 cut(s) 1758, 1893
SatI GCNGC 9 cut(s) 294, 456, 483, 534, 660, 747, 750, 849, 1038
Sau3AI GATC 4 cut(s) 42, 46, 974, 1687
Sau96I GGNCC 1 cut(s) 114
ScaI AGTACT 2 cut(s) 694, 883
SchI GAGTC 2 cut(s) 92, 1999
SduI GDGCHC 1 cut(s) 27
SexAI ACCWGGT 1 cut(s) 1525
SfaNI GCATC 9 cut(s) 214, 331, 467, 520, 697, 886, 1075, 1672, 1811
SfcI CTRYAG 1 cut(s) 384
SmaI CCCGGG 1 cut(s) 1489
SmiMI CAYNNNNRTG 2 cut(s) 274, 424
Sse9I AATT 7 cut(s) 65, 1314, 1543, 1719, 1735, 1773, 1885
SsiI CCGC 3 cut(s) 456, 534, 1205
SspI AATATT 1 cut(s) 1627
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 4 cut(s) 1511, 1695, 1789, 2038
TaiI ACGT 2 cut(s) 586, 1224
TaqI TCGA 2 cut(s) 45, 632
TasI AATT 7 cut(s) 65, 1314, 1543, 1719, 1735, 1773, 1885
TatI WGTACW 3 cut(s) 212, 692, 881
TauI GCSGC 2 cut(s) 458, 536
TfiI GAWTC 4 cut(s) 74, 188, 1388, 1957
Tru1I TTAA 2 cut(s) 1758, 1893
Tru9I TTAA 2 cut(s) 1758, 1893
TscAI CASTG 3 cut(s) 32, 1555, 1698
TseFI GTSAC 2 cut(s) 1834, 1847
TseI GCWGC 7 cut(s) 293, 482, 659, 746, 749, 848, 1037
Tsp45I GTSAC 2 cut(s) 1834, 1847
TspDTI ATGAA 5 cut(s) 78, 1242, 1296, 1550, 1915
TspMI CCCGGG 1 cut(s) 1487
TspRI CASTG 3 cut(s) 32, 1555, 1698
XapI RAATTY 1 cut(s) 1314
XbaI TCTAGA 3 cut(s) 106, 731, 920
XmaI CCCGGG 1 cut(s) 1487
ZrmI AGTACT 2 cut(s) 694, 883
Zsp2I ATGCAT 1 cut(s) 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.