pycom07g03250

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0028859
Physical Location & Seq
Reverse (-)
2717939 .. 2720119
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03250.2

Sequence Viewer

Length: 1722 bp
ATGGCTGCAGCTTTGGATGCCGGAGAGGCTTCTCTCTCTGCTTCCATCCAGCAGCTCTGCAAACGGATTGATTCGCGCGAAATCAGGGACATTTTTGGGCCAAAGAAGCTTGACAAGTCACTCCTGAAGAAATTCAAGCTGACGTTGTTAACCCTTGACGCAGTGCTCGATGACGCACACCAGAAGGAGTTTGAGATCCCTTCCGTCGGAAACTGGCTTGATGAATTGAGAGAAGCGGTCTGTGATGCGGGCGTCCTGCTGGATGATATCGATGCTGAAGCTTTCGGGCTTAAGATGGCAGCAGAAGATCATTCCGTTGTAACTCAGGTGTGGAACTTCCTCTCTGTGCCTTTCAGTCGTTTTGACCAACGCATGAAAGATAAGATAAAGGAGTTATATCATAGATTAAAATTCCTTGGAAACGAAAAGGATGTTCTTGGTCTCCGAAAGGGTGTTCGGAAGGTTTCACAGATGCCAACAACTTGTTTGGTTGATAAATCTTTTGTAGTTGGTAGAGATGGTGATAAAGAAGACGTGACAAGACTGTTGATCTCTGATGCCGAAAGCAGGAATAATGTTTCTGTCATTACTATAGTGGGAATGGCCGGGATTGGTAAGACAACACTTGCTCAACTCGTATACAACGATCAAAGAGTGAAGGAGCATTTTGATATTCAAGCTTGGGTTTGCGTTTCCGAAGATTTTGATGCTGTTAGGGTCACTAAAACCCTGCTCGAGTCAATCACTTCGACTGTTTGTGACATTACCGATCTAAATTTTGTTCAGGTCCAGCTAAAACACGAAGTGAGGGGAAAGAGATTCTTATTTGTGTTGGACGACCTCTGGAACGAGAACTATCATGATTGGAATCTCCTGCAAGTTCCTTTTCTCTATGCGGCAGGGGGAAGTAAGGTTGTGATAACAACGCGGAGTGAAATGGTTGCTTCAATCGTGCGCAATGTACCTACTCACTATTTAGAGCCGCTGTCCGATGAAGATTGTTGGTCGTTGATTTTAAAACATGCTTTGGGAGACGGTGACACCGCTGAACATTCAAACATTCGAGAAACTGATAGGGAAACTGCAATACGTAAGTGCAATGGTTTGCCTTTAATTGCACGAGCACTAGGGGGTCTCTTACGAGGCACAACTTGTGGTTCTGAGGAATGGCATCACATATTACATACCAACATTTGGGAGCTACCCTCCATTGCTCGCATTCTCCCAATTTGTTTACTACAACAATTAAGGCAGAAGGGGAGAGAATCACATCAAAAACGCCGCTCTGCTAGGACAGAGGCAAATTCAGCAGAGTGGATCTTTTTAGTCACTAGCCTTTGCATGGAGATTTTATCAGCTGCTTTCGATCAGGCTTCGTCCCCAAGAAGGCCGAGGTATGCCCTGTTCGGAATGCTCTTGGCTATTGCAGCTCTACTCACTTGCATATGGGAGCTGATTTACAAAGGCAGGAAAAATAACGTGGTGTGGAGGAAGAGGGGATGTTACATGCTTTTTGGTTCTGCCAATGAAATTTTTGGGTTACTCGGAGGTATCGCTCAGTGTGTTTGCTCTATAGTTCAATACTTTTATTACATTCGGCGTGCTAATAATCCTCTCAAACTGTCCCTTTTGCCTACCATCTTTCTCATATGTTTGATTGCTGCAAGATTAAGTAGGAAACGAATGCAGACCCCAGATGAGACCGGTGAACATACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
FNIP LRR_8 NB-ARC

Protein Analysis

574

Amino Acids

64.73

Weight (kDa)

7.86

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028859)

Species Orthologous Gene IDs
malus_domestica MD07G1045400.v1.1
pyrus_communis pycom07g03250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 956
AccB7I CCANNNNNTGG 1 cut(s) 1194
AccBSI CCGCTC 1 cut(s) 1284
AccI GTMKAC 1 cut(s) 639
AccII CGCG 3 cut(s) 76, 78, 928
AciI CCGC 7 cut(s) 236, 248, 896, 928, 983, 1044, 1282
AclWI GGATC 2 cut(s) 190, 1325
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 5 cut(s) 131, 410, 775, 1303, 1530
AcuI CTGAAG 2 cut(s) 146, 297
AcyI GRCGYC 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 805
AfaI GTAC 1 cut(s) 963
AfiI CCNNNNNNNGG 5 cut(s) 206, 567, 1194, 1342, 1386
AflII CTTAAG 1 cut(s) 290
AgeI ACCGGT 1 cut(s) 1703
AgsI TTSAA 5 cut(s) 136, 677, 948, 1056, 1580
AjiI CACGTC 1 cut(s) 535
Alw21I GWGCWC 2 cut(s) 168, 1126
Alw26I GTCTC 4 cut(s) 446, 1026, 1139, 1694
AlwI GGATC 2 cut(s) 190, 1325
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 3 cut(s) 98, 603, 1388
ApeKI GCWGC 7 cut(s) 5, 8, 52, 299, 1358, 1427, 1661
ApoI RAATTY 5 cut(s) 131, 410, 775, 1303, 1530
AsiGI ACCGGT 1 cut(s) 1703
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 2 cut(s) 78, 957
AspS9I GGNCC 2 cut(s) 98, 787
AsuC2I CCSGG 1 cut(s) 607
AsuHPI GGTGA 3 cut(s) 533, 1049, 1718
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 1 cut(s) 787
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 2 cut(s) 168, 1126
BbvI GCAGC 6 cut(s) 20, 64, 311, 1345, 1439, 1648
BccI CCATC 4 cut(s) 53, 289, 512, 1646
BcnI CCSGG 1 cut(s) 607
BcoDI GTCTC 4 cut(s) 446, 1026, 1139, 1694
BfaI CTAG 3 cut(s) 1127, 1290, 1332
BfmI CTRYAG 3 cut(s) 6, 591, 1572
BfrI CTTAAG 1 cut(s) 290
BglI GCCNNNNNGGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 607
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 734
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 98, 787
BmrFI CCNGG 1 cut(s) 607
BmsI GCATC 7 cut(s) 7, 235, 262, 462, 547, 697, 1180
BpiI GAAGAC 1 cut(s) 537
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BpuMI CCSGG 1 cut(s) 607
Bsa29I ATCGAT 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 1091
BsaBI GATNNNNATC 1 cut(s) 867
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 3 cut(s) 446, 1139, 1694
BsaJI CCNNGG 2 cut(s) 415, 1391
BsaWI WCCGGW 1 cut(s) 1703
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 567, 1194, 1342, 1386
Bse118I RCCGGY 1 cut(s) 1703
Bse1I ACTGG 1 cut(s) 218
Bse3DI GCAATG 3 cut(s) 964, 1105, 1209
Bse8I GATNNNNATC 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 415, 1391
BseGI GGATG 5 cut(s) 22, 45, 268, 436, 1505
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 5 cut(s) 206, 567, 1194, 1342, 1386
BseMI GCAATG 3 cut(s) 964, 1105, 1209
BseMII CTCAG 3 cut(s) 338, 1152, 1571
BseNI ACTGG 1 cut(s) 218
BseXI GCAGC 6 cut(s) 20, 64, 311, 1345, 1439, 1648
Bsh1236I CGCG 3 cut(s) 76, 78, 928
BshFI GGCC 3 cut(s) 100, 605, 1390
BshTI ACCGGT 1 cut(s) 1703
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 168, 1126
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 3 cut(s) 21, 606, 1704
BslFI GGGAC 3 cut(s) 101, 1363, 1609
BslI CCNNNNNNNGG 5 cut(s) 206, 567, 1194, 1342, 1386
BsmAI GTCTC 4 cut(s) 446, 1026, 1139, 1694
BsmBI CGTCTC 1 cut(s) 1026
BsmFI GGGAC 3 cut(s) 101, 1363, 1609
BsmI GAATGC 3 cut(s) 1218, 1416, 1689
BsnI GGCC 3 cut(s) 100, 605, 1390
Bso31I GGTCTC 3 cut(s) 446, 1139, 1694
BsoBI CYCGRG 1 cut(s) 734
Bsp1286I GDGCHC 2 cut(s) 168, 1126
Bsp143I GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
BspACI CCGC 7 cut(s) 236, 248, 896, 928, 983, 1044, 1282
BspANI GGCC 3 cut(s) 100, 605, 1390
BspCNI CTCAG 3 cut(s) 337, 1153, 1570
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 3 cut(s) 76, 78, 928
BspHI TCATGA 1 cut(s) 859
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 190, 1325
BspTI CTTAAG 1 cut(s) 290
BspTNI GGTCTC 3 cut(s) 446, 1139, 1694
BsrBI CCGCTC 1 cut(s) 1284
BsrDI GCAATG 3 cut(s) 964, 1105, 1209
BsrFI RCCGGY 1 cut(s) 1703
BsrI ACTGG 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 1703
BssECI CCNNGG 2 cut(s) 415, 1391
BssMI GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
BssNAI GTATAC 1 cut(s) 640
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 415
Bst1107I GTATAC 1 cut(s) 640
Bst2BI CACGAG 1 cut(s) 1119
Bst4CI ACNGT 4 cut(s) 546, 754, 1037, 1623
Bst6I CTCTTC 1 cut(s) 1487
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 1091
BstC8I GCNNGC 3 cut(s) 250, 1216, 1602
BstDEI CTNAG 4 cut(s) 324, 1161, 1557, 1719
BstF5I GGATG 5 cut(s) 22, 45, 268, 436, 1505
BstFNI CGCG 3 cut(s) 76, 78, 928
BstHHI GCGC 2 cut(s) 78, 957
BstKTI GATC 7 cut(s) 198, 310, 552, 649, 772, 1320, 1369
BstMAI GTCTC 4 cut(s) 446, 1026, 1139, 1694
BstMBI GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
BstMWI GCNNNNNNNGC 5 cut(s) 17, 26, 106, 1307, 1427
BstNSI RCATGY 2 cut(s) 1025, 1510
BstSCI CCNGG 1 cut(s) 605
BstSFI CTRYAG 3 cut(s) 6, 591, 1572
BstSNI TACGTA 1 cut(s) 1091
BstUI CGCG 3 cut(s) 76, 78, 928
BstV1I GCAGC 6 cut(s) 20, 64, 311, 1345, 1439, 1648
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 2 cut(s) 195, 1317
BstYI RGATCY 2 cut(s) 195, 1317
BstZ17I GTATAC 1 cut(s) 640
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 3 cut(s) 100, 605, 1390
BsuTUI ATCGAT 1 cut(s) 270
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 5 cut(s) 22, 45, 268, 436, 1505
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 168, 1565
Cac8I GCNNGC 3 cut(s) 250, 1216, 1602
CciI TCATGA 1 cut(s) 859
CfoI GCGC 2 cut(s) 78, 957
Cfr10I RCCGGY 1 cut(s) 1703
Cfr13I GGNCC 2 cut(s) 98, 787
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 3 cut(s) 167, 182, 241
Csp6I GTAC 1 cut(s) 962
CspAI ACCGGT 1 cut(s) 1703
CviAII CATG 5 cut(s) 373, 860, 1022, 1342, 1507
CviQI GTAC 1 cut(s) 962
DdeI CTNAG 4 cut(s) 324, 1161, 1557, 1719
DpnI GATC 7 cut(s) 197, 309, 551, 648, 771, 1319, 1368
DpnII GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
DraI TTTAAA 1 cut(s) 1017
DraIII CACNNNGTG 1 cut(s) 805
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 1 cut(s) 1487
EarI CTCTTC 1 cut(s) 1487
Eco105I TACGTA 1 cut(s) 1091
Eco130I CCWWGG 1 cut(s) 415
Eco31I GGTCTC 3 cut(s) 446, 1139, 1694
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 2 cut(s) 146, 297
Eco88I CYCGRG 1 cut(s) 734
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
Esp3I CGTCTC 1 cut(s) 1026
FaeI CATG 5 cut(s) 376, 863, 1025, 1345, 1510
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 3 cut(s) 101, 1363, 1609
FatI CATG 5 cut(s) 372, 859, 1021, 1341, 1506
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 2 cut(s) 1445, 1649
FblI GTMKAC 1 cut(s) 639
FokI GGATG 5 cut(s) 29, 32, 275, 443, 1512
FspBI CTAG 3 cut(s) 1127, 1290, 1332
FspI TGCGCA 1 cut(s) 956
GlaI GCGC 2 cut(s) 77, 956
HaeIII GGCC 3 cut(s) 100, 605, 1390
HapII CCGG 3 cut(s) 21, 606, 1704
HgaI GACGC 3 cut(s) 167, 182, 241
HhaI GCGC 2 cut(s) 78, 957
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 5 cut(s) 376, 863, 1025, 1345, 1510
Hin6I GCGC 2 cut(s) 76, 955
HinP1I GCGC 2 cut(s) 76, 955
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 3 cut(s) 107, 279, 678
HinfI GANTC 5 cut(s) 71, 737, 819, 868, 1265
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 3 cut(s) 21, 606, 1704
HphI GGTGA 3 cut(s) 533, 1049, 1718
Hpy166II GTNNAC 4 cut(s) 150, 640, 1235, 1709
Hpy188I TCNGA 9 cut(s) 209, 446, 459, 556, 697, 991, 1162, 1409, 1547
Hpy188III TCNNGA 4 cut(s) 124, 844, 860, 1064
Hpy8I GTNNAC 4 cut(s) 150, 640, 1235, 1709
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 6 cut(s) 178, 210, 454, 652, 1249, 1380
HpyCH4III ACNGT 4 cut(s) 546, 754, 1037, 1623
HpyCH4IV ACGT 4 cut(s) 143, 534, 1090, 1479
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 26, 106, 1307, 1427
HpyF3I CTNAG 4 cut(s) 324, 1161, 1557, 1719
HpySE526I ACGT 4 cut(s) 143, 534, 1090, 1479
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 5 cut(s) 376, 863, 1025, 1345, 1510
HspAI GCGC 2 cut(s) 76, 955
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
LmnI GCTCC 3 cut(s) 661, 1198, 1450
Lsp1109I GCAGC 6 cut(s) 20, 64, 311, 1345, 1439, 1648
LweI GCATC 7 cut(s) 7, 235, 262, 462, 547, 697, 1180
MaeI CTAG 3 cut(s) 1127, 1290, 1332
MaeII ACGT 4 cut(s) 143, 534, 1090, 1479
MaeIII GTNAC 9 cut(s) 117, 319, 535, 718, 758, 1037, 1327, 1502, 1539
MalI GATC 7 cut(s) 197, 309, 551, 648, 771, 1319, 1368
MbiI CCGCTC 1 cut(s) 1284
MboI GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
MboII GAAGA 6 cut(s) 139, 317, 542, 710, 1007, 1504
MflI RGATCY 2 cut(s) 195, 1317
MhlI GDGCHC 2 cut(s) 168, 1126
MluCI AATT 9 cut(s) 131, 224, 410, 775, 1113, 1227, 1244, 1303, 1530
MlyI GAGTC 1 cut(s) 746
MmeI TCCRAC 2 cut(s) 187, 813
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 7 cut(s) 149, 291, 407, 1016, 1112, 1247, 1670
MspA1I CMGCKG 3 cut(s) 985, 1046, 1358
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 3 cut(s) 21, 606, 1704
MspR9I CCNGG 1 cut(s) 607
Mva1269I GAATGC 3 cut(s) 1218, 1416, 1689
MvnI CGCG 3 cut(s) 76, 78, 928
MwoI GCNNNNNNNGC 5 cut(s) 17, 26, 106, 1307, 1427
NciI CCSGG 1 cut(s) 607
NdeI CATATG 2 cut(s) 1445, 1649
NdeII GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
NlaIII CATG 5 cut(s) 376, 863, 1025, 1345, 1510
NmeAIII GCCGAG 1 cut(s) 1416
NmuCI GTSAC 6 cut(s) 117, 535, 718, 758, 1037, 1327
NsbI TGCGCA 1 cut(s) 956
NspI RCATGY 2 cut(s) 1025, 1510
PaeR7I CTCGAG 1 cut(s) 734
PagI TCATGA 1 cut(s) 859
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 642
PctI GAATGC 3 cut(s) 1218, 1416, 1689
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 4 cut(s) 71, 819, 868, 1265
PflMI CCANNNNNTGG 1 cut(s) 1194
PinAI ACCGGT 1 cut(s) 1703
PleI GAGTC 1 cut(s) 745
PpsI GAGTC 1 cut(s) 745
Ppu21I YACGTR 1 cut(s) 1091
PspPI GGNCC 2 cut(s) 98, 787
PspXI VCTCGAGB 1 cut(s) 734
PsrI GAACNNNNNNTAC 2 cut(s) 1388, 1420
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 195, 1317
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 1 cut(s) 963
RsaNI GTAC 1 cut(s) 962
SaqAI TTAA 7 cut(s) 149, 291, 407, 1016, 1112, 1247, 1670
Sau3AI GATC 7 cut(s) 195, 307, 549, 646, 769, 1317, 1366
Sau96I GGNCC 2 cut(s) 98, 787
SchI GAGTC 1 cut(s) 746
ScrFI CCNGG 1 cut(s) 607
SduI GDGCHC 2 cut(s) 168, 1126
SfaNI GCATC 7 cut(s) 7, 235, 262, 462, 547, 697, 1180
SfcI CTRYAG 3 cut(s) 6, 591, 1572
Sfr274I CTCGAG 1 cut(s) 734
SinI GGWCC 1 cut(s) 787
SlaI CTCGAG 1 cut(s) 734
SmlI CTYRAG 2 cut(s) 290, 734
SmoI CTYRAG 2 cut(s) 290, 734
SnaBI TACGTA 1 cut(s) 1091
Sse9I AATT 9 cut(s) 131, 224, 410, 775, 1113, 1227, 1244, 1303, 1530
SsiI CCGC 7 cut(s) 236, 248, 896, 928, 983, 1044, 1282
SspMI CTAG 3 cut(s) 1127, 1290, 1332
StyD4I CCNGG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 4 cut(s) 546, 754, 1037, 1623
TaiI ACGT 4 cut(s) 146, 537, 1093, 1482
TaqI TCGA 6 cut(s) 168, 270, 735, 749, 1063, 1365
TasI AATT 9 cut(s) 131, 224, 410, 775, 1113, 1227, 1244, 1303, 1530
TauI GCSGC 3 cut(s) 899, 985, 1284
TfiI GAWTC 4 cut(s) 71, 819, 868, 1265
Tru1I TTAA 7 cut(s) 149, 291, 407, 1016, 1112, 1247, 1670
Tru9I TTAA 7 cut(s) 149, 291, 407, 1016, 1112, 1247, 1670
TscAI CASTG 2 cut(s) 168, 1565
TseFI GTSAC 6 cut(s) 117, 535, 718, 758, 1037, 1327
TseI GCWGC 7 cut(s) 5, 8, 52, 299, 1358, 1427, 1661
Tsp45I GTSAC 6 cut(s) 117, 535, 718, 758, 1037, 1327
TspDTI ATGAA 4 cut(s) 237, 389, 1008, 1542
TspGWI ACGGA 3 cut(s) 79, 193, 304
TspRI CASTG 2 cut(s) 168, 1565
Van91I CCANNNNNTGG 1 cut(s) 1194
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 5 cut(s) 131, 410, 775, 1303, 1530
XceI RCATGY 2 cut(s) 1025, 1510
XhoI CTCGAG 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 3 cut(s) 1127, 1290, 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.