pycom07g04390

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0024481
Physical Location & Seq
Forward (+)
3787007 .. 3789088
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1428 bp
ATGTCTGAAGTGGGAGATCCAGCCATTAAGCTCTTCGGGAGGATTATTCCGTTCCCACAACTTCAGATTCAAGATTTGTCCGCCGACAAAGAGTGTTTGGTTGCAGATACGGGTGAGTGTAGAGAAATGGAGGACCATTCTGTAGAAAAATCCTGCAAGCAAGATACATCTTCGGAACTAGATAATAGCAGAGAAAACAATCGCCAAGGTCTAGTCCAGGAGAATGAGGGTGAAGTTAATTGTGATCAAGGAGAAGACCAATGCGTGGATAGTGATACGAGTGGCATAGAAAAGTCTTTCAGAAAGCCAGACAAGGTTCTTCCTTGCCCTCGTTGCAACAGCTTAGAAACAAAGTTTTGCTATTTCAATAATTACAGTGTTTACCAACCGAGACACTTTTGCAAGAGCTGCCAAAGATATTGGACTGCCGGGGGAACCATAAGAAATGTTCCTCTAGGTACTAGGCGGCGCAAGAATAAGCATTCATCTTTGCAGTATCATCAGGTAGTGGGACAACCGAATGCAGTACCAGTCACCCAGGGAGGCACCCTTAACATTGTTGGCCAACAGCATCTATCACCTTGTGGACTTCCAGCCTCTCCAAATCCTGCCAATGGGATAAAGGAAGGCTTAAGTTCTGTCTCAGATGCGCTTTTAGTTGAATCTACAGAGACTGCCTCAAACCAGAAACACCAGATGAGAAATTCTGAGACTGGCTCTGCAGCTGACGGCGATGATGGTTCCTTATCAGCCACTGCTTCACAGCACATTGCGTGTCTGACAAAAGAAATGAAGCAGGATGGTCTGCCAGGATATGACAATGATTCTAATCATATGCATACTATGCAATATTTCCCTGCTCCTCAATGGGACAAGACTCCGAGTTGGGGCATAATGATGTTTGGATCTGAAAGCAGTAATCCTAATCCTTCTCATATAGGTCATCTGCCTATGGTTACAGTTCCTGGTTTTTGTACTCCCACTTTCGCTTTCCCATTTGTGCCTTCCTTGCACTGGGGTTACATGCCAAGCTGGGACTCCAGAAAATGGAATTCTCAGATGGTTGGATCTACTGGCAGCTTGTCACTTTCGTCTTTCTCTGGCAACAGCGGATGTTCGGGCAATAGCTCGCCAACCTTGGGAAAATATGCAAGAGATGCAAATGTACATGAAGATGTAATAACAAAACAGTGCCTTTGGGTACCCAAGACTTTGAGAATTGATGACCCAGAAGAGGCTGCAAAGAGTTCTATATGGTCTACCTTGCGTATCAAGCAGGATAAGAATGAACTTGTAGTTAGAGGTCGTATTTTTAAAGCTTTCAGAAAGAAATCGGATAATAAATCCCAAACATTAGATGCTGATCGGGTTTTACAGGCAAATCCAGCAGCTCTCTCTCGCTCTCAATCCTTCCAGGAGACCGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

476

Amino Acids

52.38

Weight (kDa)

6.24

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 105 - 161 8.4e-31 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024481)

Species Orthologous Gene IDs
malus_domestica MD07G1057700.v1.1
prunus_persica Prupe.2G063800_v2.0.a1
pyrus_communis pycom07g04390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1201
AccB1I GGYRCC 2 cut(s) 545, 1201
AccB7I CCANNNNNTGG 2 cut(s) 265, 1047
AccI GTMKAC 1 cut(s) 1259
AccII CGCG 1 cut(s) 1424
AciI CCGC 4 cut(s) 81, 466, 1110, 1422
AclWI GGATC 3 cut(s) 11, 913, 1075
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 2 cut(s) 703, 1051
AcuI CTGAAG 2 cut(s) 27, 47
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 5 cut(s) 460, 528, 976, 1167, 1203
AfiI CCNNNNNNNGG 7 cut(s) 265, 614, 887, 1014, 1047, 1139, 1234
AflII CTTAAG 1 cut(s) 631
AgsI TTSAA 3 cut(s) 71, 367, 662
AjnI CCWGG 5 cut(s) 216, 537, 808, 964, 1413
AluBI AGCT 9 cut(s) 31, 342, 408, 725, 1032, 1080, 1128, 1319, 1391
AluI AGCT 9 cut(s) 31, 342, 408, 725, 1032, 1080, 1128, 1319, 1391
Alw26I GTCTC 5 cut(s) 385, 646, 665, 704, 1412
AlwI GGATC 3 cut(s) 11, 913, 1075
AlwNI CAGNNNCTG 3 cut(s) 674, 755, 965
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 5 cut(s) 408, 722, 1077, 1238, 1388
ApoI RAATTY 2 cut(s) 703, 1051
Asp718I GGTACC 1 cut(s) 1201
AspLEI GCGC 2 cut(s) 471, 652
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 4 cut(s) 125, 242, 526, 570
AvaII GGWCC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 564
BanI GGYRCC 2 cut(s) 545, 1201
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 395, 734, 1089, 1225, 1400
BccI CCATC 3 cut(s) 731, 794, 1054
BceAI ACGGC 1 cut(s) 745
BciT130I CCWGG 5 cut(s) 218, 539, 810, 966, 1415
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 5 cut(s) 385, 646, 665, 704, 1412
BfaI CTAG 4 cut(s) 179, 212, 455, 462
BfmI CTRYAG 3 cut(s) 141, 666, 720
BfrI CTTAAG 1 cut(s) 631
BisI GCNGC 6 cut(s) 409, 467, 723, 1078, 1239, 1389
BlsI GCNGC 6 cut(s) 410, 468, 724, 1079, 1240, 1390
Bme1390I CCNGG 6 cut(s) 218, 430, 539, 810, 966, 1415
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 4 cut(s) 436, 547, 742, 1203
BmrFI CCNGG 6 cut(s) 218, 430, 539, 810, 966, 1415
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 4 cut(s) 580, 637, 1147, 1348
BmuI ACTGGG 1 cut(s) 1024
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 4 cut(s) 662, 694, 701, 733
BpmI CTGGAG 1 cut(s) 1024
BpuMI CCSGG 1 cut(s) 430
BsaBI GATNNNNATC 2 cut(s) 828, 1362
BsaI GGTCTC 1 cut(s) 1412
BsaJI CCNNGG 5 cut(s) 205, 429, 537, 538, 1137
BsaXI ACNNNNNCTCC 1 cut(s) 1409
Bsc4I CCNNNNNNNGG 7 cut(s) 265, 614, 887, 1014, 1047, 1139, 1234
Bse1I ACTGG 4 cut(s) 530, 718, 1019, 1078
Bse3DI GCAATG 1 cut(s) 768
Bse8I GATNNNNATC 2 cut(s) 828, 1362
BseBI CCWGG 5 cut(s) 218, 539, 810, 966, 1415
BseDI CCNNGG 5 cut(s) 205, 429, 537, 538, 1137
BseGI GGATG 2 cut(s) 805, 1118
BseJI GATNNNNATC 2 cut(s) 828, 1362
BseLI CCNNNNNNNGG 7 cut(s) 265, 614, 887, 1014, 1047, 1139, 1234
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 3 cut(s) 657, 699, 1070
BseNI ACTGG 4 cut(s) 530, 718, 1019, 1078
BseRI GAGGAG 1 cut(s) 852
BseXI GCAGC 5 cut(s) 395, 734, 1089, 1225, 1400
BseYI CCCAGC 1 cut(s) 1032
Bsh1236I CGCG 1 cut(s) 1424
BshFI GGCC 1 cut(s) 564
BshNI GGYRCC 2 cut(s) 545, 1201
BsiSI CCGG 1 cut(s) 429
BslFI GGGAC 3 cut(s) 525, 884, 1049
BslI CCNNNNNNNGG 7 cut(s) 265, 614, 887, 1014, 1047, 1139, 1234
BsmAI GTCTC 5 cut(s) 385, 646, 665, 704, 1412
BsmFI GGGAC 3 cut(s) 525, 884, 1049
BsmI GAATGC 2 cut(s) 481, 526
BsnI GGCC 1 cut(s) 564
Bso31I GGTCTC 1 cut(s) 1412
Bsp1407I TGTACA 1 cut(s) 1165
Bsp143I GATC 5 cut(s) 16, 244, 905, 1067, 1363
BspACI CCGC 4 cut(s) 81, 466, 1110, 1422
BspANI GGCC 1 cut(s) 564
BspCNI CTCAG 3 cut(s) 656, 700, 1069
BspFNI CGCG 1 cut(s) 1424
BspLI GGNNCC 4 cut(s) 436, 547, 742, 1203
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 3 cut(s) 11, 913, 1075
BspQI GCTCTTC 1 cut(s) 38
BspT107I GGYRCC 2 cut(s) 545, 1201
BspTI CTTAAG 1 cut(s) 631
BspTNI GGTCTC 1 cut(s) 1412
BsrDI GCAATG 1 cut(s) 768
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 4 cut(s) 530, 718, 1019, 1078
BssECI CCNNGG 5 cut(s) 205, 429, 537, 538, 1137
BssMI GATC 5 cut(s) 16, 244, 905, 1067, 1363
BssT1I CCWWGG 2 cut(s) 205, 1137
Bst2UI CCWGG 5 cut(s) 218, 539, 810, 966, 1415
Bst4CI ACNGT 3 cut(s) 377, 961, 1191
Bst6I CTCTTC 2 cut(s) 38, 1227
BstAFI CTTAAG 1 cut(s) 631
BstAPI GCANNNNNTGC 3 cut(s) 408, 844, 1157
BstAUI TGTACA 1 cut(s) 1165
BstC8I GCNNGC 2 cut(s) 158, 1130
BstDEI CTNAG 4 cut(s) 343, 643, 708, 1056
BstF5I GGATG 2 cut(s) 805, 1118
BstFNI CGCG 1 cut(s) 1424
BstHHI GCGC 2 cut(s) 471, 652
BstKTI GATC 5 cut(s) 19, 247, 908, 1070, 1366
BstMAI GTCTC 5 cut(s) 385, 646, 665, 704, 1412
BstMBI GATC 5 cut(s) 16, 244, 905, 1067, 1363
BstMWI GCNNNNNNNGC 7 cut(s) 333, 408, 844, 1009, 1157, 1273, 1385
BstNI CCWGG 5 cut(s) 218, 539, 810, 966, 1415
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 6 cut(s) 216, 428, 537, 808, 964, 1413
BstSFI CTRYAG 3 cut(s) 141, 666, 720
BstUI CGCG 1 cut(s) 1424
BstV1I GCAGC 5 cut(s) 395, 734, 1089, 1225, 1400
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 3 cut(s) 16, 905, 1067
BstYI RGATCY 3 cut(s) 16, 905, 1067
BsuRI GGCC 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 2 cut(s) 805, 1118
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 4 cut(s) 382, 753, 1012, 1196
Cac8I GCNNGC 2 cut(s) 158, 1130
CaiI CAGNNNCTG 3 cut(s) 674, 755, 965
CfoI GCGC 2 cut(s) 471, 652
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 5 cut(s) 459, 527, 975, 1166, 1202
CviAII CATG 2 cut(s) 1024, 1169
CviQI GTAC 5 cut(s) 459, 527, 975, 1166, 1202
DdeI CTNAG 4 cut(s) 343, 643, 708, 1056
DpnI GATC 5 cut(s) 18, 246, 907, 1069, 1365
DpnII GATC 5 cut(s) 16, 244, 905, 1067, 1363
DraI TTTAAA 1 cut(s) 1315
DraIII CACNNNGTG 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 2 cut(s) 38, 1227
EarI CTCTTC 2 cut(s) 38, 1227
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 205, 1137
Eco31I GGTCTC 1 cut(s) 1412
Eco47I GGWCC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 27, 47
EcoRI GAATTC 1 cut(s) 1051
EcoRII CCWGG 5 cut(s) 216, 537, 808, 964, 1413
EcoT14I CCWWGG 2 cut(s) 205, 1137
EcoT22I ATGCAT 1 cut(s) 840
ErhI CCWWGG 2 cut(s) 205, 1137
FaeI CATG 2 cut(s) 1027, 1172
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 3 cut(s) 525, 884, 1049
FatI CATG 2 cut(s) 1023, 1168
FauNDI CATATG 1 cut(s) 834
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 1259
Fnu4HI GCNGC 6 cut(s) 409, 467, 723, 1078, 1239, 1389
FokI GGATG 2 cut(s) 812, 1125
Fsp4HI GCNGC 6 cut(s) 409, 467, 723, 1078, 1239, 1389
FspBI CTAG 4 cut(s) 179, 212, 455, 462
GlaI GCGC 2 cut(s) 470, 651
GluI GCNGC 6 cut(s) 409, 467, 723, 1078, 1239, 1389
GsaI CCCAGC 1 cut(s) 1036
GsuI CTGGAG 1 cut(s) 1024
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 429
HhaI GCGC 2 cut(s) 471, 652
Hin1II CATG 2 cut(s) 1027, 1172
Hin6I GCGC 2 cut(s) 469, 650
HinP1I GCGC 2 cut(s) 469, 650
HindIII AAGCTT 1 cut(s) 1317
HinfI GANTC 5 cut(s) 67, 662, 824, 877, 1037
HpaII CCGG 1 cut(s) 429
HphI GGTGA 4 cut(s) 125, 242, 526, 570
Hpy166II GTNNAC 3 cut(s) 382, 587, 1260
Hpy188III TCNNGA 3 cut(s) 37, 71, 1041
Hpy8I GTNNAC 3 cut(s) 382, 587, 1260
HpyAV CCTTC 4 cut(s) 620, 939, 1014, 1420
HpyCH4III ACNGT 3 cut(s) 377, 961, 1191
HpyF10VI GCNNNNNNNGC 7 cut(s) 333, 408, 844, 1009, 1157, 1273, 1385
HpyF3I CTNAG 4 cut(s) 343, 643, 708, 1056
Hsp92II CATG 2 cut(s) 1027, 1172
HspAI GCGC 2 cut(s) 469, 650
KpnI GGTACC 1 cut(s) 1205
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 5 cut(s) 16, 244, 905, 1067, 1363
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 865
Lsp1109I GCAGC 5 cut(s) 395, 734, 1089, 1225, 1400
LweI GCATC 4 cut(s) 580, 637, 1147, 1348
MaeI CTAG 4 cut(s) 179, 212, 455, 462
MaeIII GTNAC 4 cut(s) 532, 955, 1019, 1083
MalI GATC 5 cut(s) 18, 246, 907, 1069, 1365
MboI GATC 5 cut(s) 16, 244, 905, 1067, 1363
MboII GAAGA 6 cut(s) 25, 162, 266, 311, 1184, 1244
MflI RGATCY 3 cut(s) 16, 905, 1067
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 5 cut(s) 238, 370, 703, 1051, 1218
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 2 cut(s) 871, 1031
MmeI TCCRAC 1 cut(s) 1045
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 840
MscI TGGCCA 1 cut(s) 564
MseI TTAA 5 cut(s) 27, 237, 552, 632, 1314
MslI CAYNNNNRTG 2 cut(s) 896, 1173
Msp20I TGGCCA 1 cut(s) 564
MspA1I CMGCKG 2 cut(s) 725, 1110
MspCI CTTAAG 1 cut(s) 631
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 6 cut(s) 218, 430, 539, 810, 966, 1415
Mva1269I GAATGC 2 cut(s) 481, 526
MvaI CCWGG 5 cut(s) 218, 539, 810, 966, 1415
MvnI CGCG 1 cut(s) 1424
MwoI GCNNNNNNNGC 7 cut(s) 333, 408, 844, 1009, 1157, 1273, 1385
NciI CCSGG 1 cut(s) 430
NdeI CATATG 1 cut(s) 834
NdeII GATC 5 cut(s) 16, 244, 905, 1067, 1363
NlaIII CATG 2 cut(s) 1027, 1172
NlaIV GGNNCC 4 cut(s) 436, 547, 742, 1203
NmuCI GTSAC 2 cut(s) 532, 1083
NsiI ATGCAT 1 cut(s) 840
NspI RCATGY 1 cut(s) 1027
PasI CCCWGGG 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 2 cut(s) 481, 526
PfeI GAWTC 3 cut(s) 67, 662, 824
PflMI CCANNNNNTGG 2 cut(s) 265, 1047
PfoI TCCNGGA 2 cut(s) 216, 1413
PkrI GCNGC 6 cut(s) 410, 468, 724, 1079, 1240, 1390
PleI GAGTC 2 cut(s) 871, 1031
PpsI GAGTC 2 cut(s) 871, 1031
Psp6I CCWGG 5 cut(s) 216, 537, 808, 964, 1413
PspFI CCCAGC 1 cut(s) 1032
PspGI CCWGG 5 cut(s) 216, 537, 808, 964, 1413
PspN4I GGNNCC 4 cut(s) 436, 547, 742, 1203
PspPI GGNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 3 cut(s) 674, 755, 965
PsuI RGATCY 3 cut(s) 16, 905, 1067
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 5 cut(s) 460, 528, 976, 1167, 1203
RsaNI GTAC 5 cut(s) 459, 527, 975, 1166, 1202
RseI CAYNNNNRTG 2 cut(s) 896, 1173
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 5 cut(s) 27, 237, 552, 632, 1314
SatI GCNGC 6 cut(s) 409, 467, 723, 1078, 1239, 1389
Sau3AI GATC 5 cut(s) 16, 244, 905, 1067, 1363
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 2 cut(s) 871, 1031
ScrFI CCNGG 6 cut(s) 218, 430, 539, 810, 966, 1415
SfaNI GCATC 4 cut(s) 580, 637, 1147, 1348
SfcI CTRYAG 3 cut(s) 141, 666, 720
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 896, 1173
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 5 cut(s) 238, 370, 703, 1051, 1218
SsiI CCGC 4 cut(s) 81, 466, 1110, 1422
SspI AATATT 1 cut(s) 851
SspMI CTAG 4 cut(s) 179, 212, 455, 462
StyD4I CCNGG 6 cut(s) 216, 428, 537, 808, 964, 1413
StyI CCWWGG 2 cut(s) 205, 1137
TaaI ACNGT 3 cut(s) 377, 961, 1191
TasI AATT 5 cut(s) 238, 370, 703, 1051, 1218
TatI WGTACW 2 cut(s) 974, 1165
TauI GCSGC 1 cut(s) 469
TfiI GAWTC 3 cut(s) 67, 662, 824
Tru1I TTAA 5 cut(s) 27, 237, 552, 632, 1314
Tru9I TTAA 5 cut(s) 27, 237, 552, 632, 1314
TscAI CASTG 4 cut(s) 382, 760, 1019, 1196
TseFI GTSAC 2 cut(s) 532, 1083
TseI GCWGC 5 cut(s) 408, 722, 1077, 1238, 1388
Tsp45I GTSAC 2 cut(s) 532, 1083
TspDTI ATGAA 4 cut(s) 474, 806, 1185, 1302
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 4 cut(s) 382, 760, 1019, 1196
Van91I CCANNNNNTGG 2 cut(s) 265, 1047
Vha464I CTTAAG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 133
XapI RAATTY 2 cut(s) 703, 1051
XceI RCATGY 1 cut(s) 1027
XmiI GTMKAC 1 cut(s) 1259
XspI CTAG 4 cut(s) 179, 212, 455, 462
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.