pycom07g08450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0029318
Physical Location & Seq
Forward (+)
7804577 .. 7805969
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g08450.3

Sequence Viewer

Length: 1050 bp
ATGACACTGCACCAAAACCGTACATATAACAAACTTCATGAGAATGTCATTAGTAAAGTTCTAAACTTGCAATTTAATTTTCTATTCCATCGACTGACAAACCTTGCAGCGCTCATGAAAAACGGATCAAACAGTCAAGGTTTTTGGGTAATTTTCAAATCATTAAGGGAGGGGAAAGGATTTGGAGTGATACTGTTTGATAAGCTCTCGATCTTTCGAGATTTTTGTTCGATTCTGCTCTCTCTCTCTCTCTCTCTCTCTCTGATTTTCTCACCTGGCAATTGGAAAAGACTTGAGGAAACATGTTCCCGAGAAAGTGACACGGCAGGTGGACCAAACTGTAGATTGGGCTCGATCCTAGGTCGGCCCAAATATGGCCTTGAATTCAGACCATTTGATTGTCTAAATTTTTCTTCAACAACGCCTTTATCTTGTCAAACTTTAAATATTTTATTATTAACAATTCAAACCCAACCAACTCATTTTCCAAAACATAACATTGTTTTTGAAAAAAAAAAAAAAAAATCAGATACAGTGTTGGTTTTGTCTATGCACAAACAAACATTGTTTCTGGAAAGAAAAGAACAAAAATCCAAACGCAGTGACTTAAATTCCAAAAACAAAATAGAGTGCTTATTTTTCTTTGAATCCAAATACAGGGAATTTCATTATTTCTTTTCTAGCTTACATGTAAGAAACAGTGTGTCTATTTCATTTGGATCCAGAAACAGTGACTTAGATTCCAGAAACTGTAACTTAAATTCCAGAAACAATGACTTAAATTCCAAAAATAATAAACAGTGGCCCCGTCATTATTATGGAATAGCATGCACATCTCAAATTTATTTTCTAGAGAAACAAACGATTTGGATCTCCACTGACGAAGAAAAATTGAAAAACAATAAATTAAATAGCATGAGGATCTATATTTTTATTTCAAAGAAAAAGAAGAAGTTCTGCAAAACAATGATGAATTCCATTAATGATGATAATCTGGAACTCAACCTAAAAAAGTTTGGGGTAAAATCCACAAATCATAATTTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

40.71

Weight (kDa)

9.8

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029318)

Species Orthologous Gene IDs
pyrus_communis pycom07g08450 pycom13g24330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
AclWI GGATC 6 cut(s) 133, 349, 714, 727, 878, 929
AcsI RAATTY 8 cut(s) 383, 406, 610, 662, 760, 781, 840, 973
AfaI GTAC 1 cut(s) 22
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 374, 657
AflIII ACRYGT 2 cut(s) 302, 688
AgsI TTSAA 8 cut(s) 157, 383, 417, 467, 509, 647, 895, 939
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 2 cut(s) 205, 684
AluI AGCT 2 cut(s) 205, 684
AlwI GGATC 6 cut(s) 133, 349, 714, 727, 878, 929
AlwNI CAGNNNCTG 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 309
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 3 cut(s) 365, 376, 803
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 8 cut(s) 383, 406, 610, 662, 760, 781, 840, 973
AseI ATTAAT 1 cut(s) 981
Asp700I GAANNNNTTC 1 cut(s) 953
AspA2I CCTAGG 1 cut(s) 358
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 332, 366, 804
AsuHPI GGTGA 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 332
AvrII CCTAGG 1 cut(s) 358
BamHI GGATCC 1 cut(s) 719
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 96
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 276
BfaI CTAG 3 cut(s) 359, 681, 851
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 317
BisI GCNGC 1 cut(s) 108
BlnI CCTAGG 1 cut(s) 358
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 332
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 3 cut(s) 332, 366, 804
BmiI GGNNCC 2 cut(s) 721, 806
BmrFI CCNGG 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 314
BsaBI GATNNNNATC 2 cut(s) 869, 990
BsaJI CCNNGG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 657
Bse8I GATNNNNATC 2 cut(s) 869, 990
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 358
BseJI GATNNNNATC 2 cut(s) 869, 990
BseLI CCNNNNNNNGG 2 cut(s) 374, 657
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 3 cut(s) 367, 378, 805
BsiHKCI CYCGRG 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 374, 657
BsnI GGCC 3 cut(s) 367, 378, 805
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 6 cut(s) 125, 210, 354, 719, 870, 921
BspANI GGCC 3 cut(s) 367, 378, 805
BspHI TCATGA 2 cut(s) 37, 114
BspLI GGNNCC 2 cut(s) 721, 806
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 6 cut(s) 133, 349, 714, 727, 878, 929
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 6 cut(s) 125, 210, 354, 719, 870, 921
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 9 cut(s) 20, 134, 195, 341, 535, 701, 731, 752, 801
BstC8I GCNNGC 1 cut(s) 829
BstDEI CTNAG 1 cut(s) 736
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 6 cut(s) 128, 213, 357, 722, 873, 924
BstMBI GATC 6 cut(s) 125, 210, 354, 719, 870, 921
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 3 cut(s) 306, 692, 831
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 119
BstX2I RGATCY 3 cut(s) 719, 870, 921
BstYI RGATCY 3 cut(s) 719, 870, 921
BsuRI GGCC 3 cut(s) 367, 378, 805
BtsI GCAGTG 2 cut(s) 5, 607
BtsIMutI CAGTG 7 cut(s) 5, 540, 607, 706, 736, 806, 876
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 829
CaiI CAGNNNCTG 1 cut(s) 750
CciI TCATGA 2 cut(s) 37, 114
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 332, 366, 804
Csp6I GTAC 1 cut(s) 21
CviAII CATG 6 cut(s) 38, 115, 303, 689, 828, 916
CviJI RGCY 6 cut(s) 205, 351, 367, 378, 684, 805
CviKI_1 RGCY 6 cut(s) 205, 351, 367, 378, 684, 805
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 1 cut(s) 736
DpnI GATC 6 cut(s) 127, 212, 356, 721, 872, 923
DpnII GATC 6 cut(s) 125, 210, 354, 719, 870, 921
DraI TTTAAA 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 332
Eco47III AGCGCT 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 309
EcoRI GAATTC 2 cut(s) 383, 973
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 6 cut(s) 41, 118, 306, 692, 831, 919
FatI CATG 6 cut(s) 37, 114, 302, 688, 827, 915
Fnu4HI GCNGC 1 cut(s) 108
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 3 cut(s) 359, 681, 851
GlaI GCGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 3 cut(s) 367, 378, 805
HhaI GCGC 1 cut(s) 112
Hin1II CATG 6 cut(s) 41, 118, 306, 692, 831, 919
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 3 cut(s) 232, 647, 740
HphI GGTGA 1 cut(s) 264
Hpy166II GTNNAC 1 cut(s) 332
Hpy188I TCNGA 3 cut(s) 264, 389, 529
Hpy8I GTNNAC 1 cut(s) 332
HpyCH4III ACNGT 9 cut(s) 20, 134, 195, 341, 535, 701, 731, 752, 801
HpyCH4V TGCA 6 cut(s) 10, 70, 107, 553, 831, 960
HpyF3I CTNAG 1 cut(s) 736
Hsp92II CATG 6 cut(s) 41, 118, 306, 692, 831, 919
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 6 cut(s) 125, 210, 354, 719, 870, 921
LpnPI CCDG 9 cut(s) 261, 288, 312, 557, 643, 736, 757, 778, 980
Lsp1109I GCAGC 1 cut(s) 119
MaeI CTAG 3 cut(s) 359, 681, 851
MaeIII GTNAC 4 cut(s) 317, 602, 731, 752
MalI GATC 6 cut(s) 127, 212, 356, 721, 872, 923
MboI GATC 6 cut(s) 125, 210, 354, 719, 870, 921
MboII GAAGA 3 cut(s) 405, 896, 961
MfeI CAATTG 1 cut(s) 280
MflI RGATCY 3 cut(s) 719, 870, 921
MhlI GDGCHC 1 cut(s) 353
MnlI CCTC 3 cut(s) 163, 289, 912
MroXI GAANNNNTTC 1 cut(s) 953
MseI TTAA 9 cut(s) 75, 164, 443, 458, 608, 758, 779, 908, 981
MslI CAYNNNNRTG 2 cut(s) 42, 816
MspR9I CCNGG 1 cut(s) 276
MunI CAATTG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 276
NdeII GATC 6 cut(s) 125, 210, 354, 719, 870, 921
NlaIII CATG 6 cut(s) 41, 118, 306, 692, 831, 919
NlaIV GGNNCC 2 cut(s) 721, 806
NmuCI GTSAC 3 cut(s) 317, 602, 731
NspI RCATGY 3 cut(s) 306, 692, 831
PaeI GCATGC 1 cut(s) 831
PagI TCATGA 2 cut(s) 37, 114
PaqCI CACCTGC 1 cut(s) 317
PciI ACATGT 2 cut(s) 302, 688
PdmI GAANNNNTTC 1 cut(s) 953
PfeI GAWTC 3 cut(s) 232, 647, 740
PkrI GCNGC 1 cut(s) 109
PscI ACATGT 2 cut(s) 302, 688
PshBI ATTAAT 1 cut(s) 981
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 721, 806
PspPI GGNCC 3 cut(s) 332, 366, 804
PstNI CAGNNNCTG 1 cut(s) 750
PsuI RGATCY 3 cut(s) 719, 870, 921
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 2 cut(s) 42, 816
SaqAI TTAA 9 cut(s) 75, 164, 443, 458, 608, 758, 779, 908, 981
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 6 cut(s) 125, 210, 354, 719, 870, 921
Sau96I GGNCC 3 cut(s) 332, 366, 804
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 105, 142, 207, 277, 331, 364, 686, 1008
SfcI CTRYAG 1 cut(s) 340
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 42, 816
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SphI GCATGC 1 cut(s) 831
SspI AATATT 1 cut(s) 448
SspMI CTAG 3 cut(s) 359, 681, 851
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 9 cut(s) 20, 134, 195, 341, 535, 701, 731, 752, 801
TaqI TCGA 5 cut(s) 91, 209, 217, 230, 353
TfiI GAWTC 3 cut(s) 232, 647, 740
Tru1I TTAA 9 cut(s) 75, 164, 443, 458, 608, 758, 779, 908, 981
Tru9I TTAA 9 cut(s) 75, 164, 443, 458, 608, 758, 779, 908, 981
TscAI CASTG 7 cut(s) 12, 540, 607, 706, 736, 806, 883
TseFI GTSAC 3 cut(s) 317, 602, 731
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 3 cut(s) 317, 602, 731
TspDTI ATGAA 5 cut(s) 26, 131, 656, 702, 986
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 7 cut(s) 12, 540, 607, 706, 736, 806, 883
VpaK11BI GGWCC 1 cut(s) 332
VspI ATTAAT 1 cut(s) 981
XapI RAATTY 8 cut(s) 383, 406, 610, 662, 760, 781, 840, 973
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 3 cut(s) 306, 692, 831
XmaJI CCTAGG 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 3 cut(s) 359, 681, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.