pycom07g20460

Protein MID1-COMPLEMENTING ACTIVITY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0024479
Physical Location & Seq
Forward (+)
22539923 .. 22541113
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGGCGAGCTCAGATTTTGCAGAGGCACCGGGACTCGACGCCGTGAGCCTCACAAACACGATTGCGTCCACCGCTCAAAGCGCAAGGACACACCGCCACAACTGCCAGCAGCTCGCAGAGCATGTAAGGATGGTAGGAAACTTGCTTGAGAAGATGAAATCAACGGACTTTATGAAGCTTCCATGTACAAAGGAGCCGTTGGATGGTCTTGAAAAGGCTCTTGTAAAAGCGCTTCTACTAGTACAGGGTTGCAGAGACAACAGCTGCCTCTACATGTTTGCCCTGGGATGGAGTGTGGTGTATCAATTCCGACAAGTCCAGGCGGAGATTGACCACCACGTCGGTCTCCTAGTCTGCCCCTTGGTTTCTCTGCTTCAAGACTTGGAGGCTATTGAAGAGGATCAGAGGTTGTATACACTTGATGAACAGGACATGGAGGTTCATGGTGTGGTGTTGAAGCCTGATCGGACAATGAAAGATGCAGACATATTGGAGAGGGCGCTGTCTCGAAAATATCCCAACATGGCTTTTGATGAGGCACTCCAGGAGGAGAAGGAGAAGCTGAAAATCGAACTACGGCGAGCAATAACGATTAACGATCCTGATCAATGTCGGATAATTGAACATCTTATTCATGTAGCAGAAAATGTAGTGAGTGGTGTGCTGCCAGGAAAGAAAGTTGAGAAGCTTCTTGTCAACGAACCAAGTTATGTGGTGTCAGGGCCCCGGGTTTCAGCTGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

249

Amino Acids

27.79

Weight (kDa)

5.34

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7792 PF25055 15 - 115 3e-07 Domain of unknown function (DUF7792)
MCAfunc PF19584 20 - 154 3.9e-39 MCAfunc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024479)

Species Orthologous Gene IDs
malus_domestica MD07G1226300.v1.1
prunus_persica Prupe.2G255300_v2.0.a1
pyrus_communis pycom07g20460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 413
AciI CCGC 3 cut(s) 72, 94, 323
AclWI GGATC 2 cut(s) 408, 593
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 187, 243
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 203, 288
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 5 cut(s) 212, 377, 395, 457, 623
AhlI ACTAGT 1 cut(s) 238
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 4 cut(s) 282, 318, 543, 667
AluBI AGCT 7 cut(s) 9, 112, 178, 264, 562, 688, 737
AluI AGCT 7 cut(s) 9, 112, 178, 264, 562, 688, 737
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 249, 350, 510
AlwI GGATC 2 cut(s) 408, 593
Ama87I CYCGRG 1 cut(s) 726
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 1 cut(s) 722
ApaI GGGCCC 1 cut(s) 726
ApeKI GCWGC 3 cut(s) 109, 264, 664
AspLEI GCGC 3 cut(s) 83, 232, 502
AspS9I GGNCC 2 cut(s) 722, 723
AsuC2I CCSGG 3 cut(s) 30, 727, 728
AvaI CYCGRG 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 726
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 11, 726
BarI GAAGNNNNNNTAC 2 cut(s) 216, 248
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 121, 251, 651
BccI CCATC 3 cut(s) 124, 197, 282
BceAI ACGGC 3 cut(s) 26, 181, 593
BciT130I CCWGG 4 cut(s) 284, 320, 545, 669
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 3 cut(s) 30, 727, 728
BcoDI GTCTC 3 cut(s) 249, 350, 510
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 239, 350
BfoI RGCGCY 2 cut(s) 233, 503
BisI GCNGC 3 cut(s) 110, 265, 665
BlsI GCNGC 3 cut(s) 111, 266, 666
Bme1390I CCNGG 7 cut(s) 30, 284, 320, 545, 669, 727, 728
BmeT110I CYCGRG 1 cut(s) 726
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 722, 723
BmiI GGNNCC 4 cut(s) 27, 195, 724, 725
BmrFI CCNGG 7 cut(s) 30, 284, 320, 545, 669, 727, 728
BmsI GCATC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 3 cut(s) 30, 727, 728
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 5 cut(s) 282, 283, 360, 725, 726
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 288
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 4 cut(s) 284, 320, 545, 669
BseDI CCNNGG 5 cut(s) 282, 283, 360, 725, 726
BseGI GGATG 3 cut(s) 135, 208, 293
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 2 cut(s) 203, 288
BseMII CTCAG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 726
BseXI GCAGC 3 cut(s) 121, 251, 651
BshFI GGCC 1 cut(s) 724
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 726
BsiSI CCGG 2 cut(s) 29, 727
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 203, 288
BsmAI GTCTC 3 cut(s) 249, 350, 510
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 1 cut(s) 724
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 726
Bsp120I GGGCCC 1 cut(s) 722
Bsp1286I GDGCHC 2 cut(s) 11, 726
Bsp1407I TGTACA 1 cut(s) 185
Bsp143I GATC 4 cut(s) 400, 463, 598, 604
BspACI CCGC 3 cut(s) 72, 94, 323
BspANI GGCC 1 cut(s) 724
BspCNI CTCAG 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 27, 195, 724, 725
BspPI GGATC 2 cut(s) 408, 593
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 185
BssECI CCNNGG 5 cut(s) 282, 283, 360, 725, 726
BssMI GATC 4 cut(s) 400, 463, 598, 604
BssNAI GTATAC 1 cut(s) 414
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 414
Bst2UI CCWGG 4 cut(s) 284, 320, 545, 669
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 39
BstAUI TGTACA 1 cut(s) 185
BstC8I GCNNGC 4 cut(s) 7, 107, 114, 582
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 3 cut(s) 135, 208, 293
BstH2I RGCGCY 2 cut(s) 233, 503
BstHHI GCGC 3 cut(s) 83, 232, 502
BstKTI GATC 4 cut(s) 403, 466, 601, 607
BstMAI GTCTC 3 cut(s) 249, 350, 510
BstMBI GATC 4 cut(s) 400, 463, 598, 604
BstMWI GCNNNNNNNGC 4 cut(s) 71, 80, 102, 118
BstNI CCWGG 4 cut(s) 284, 320, 545, 669
BstNSI RCATGY 2 cut(s) 125, 277
BstSCI CCNGG 7 cut(s) 28, 282, 318, 543, 667, 725, 726
BstSLI GKGCMC 1 cut(s) 726
BstV1I GCAGC 3 cut(s) 121, 251, 651
BstZ17I GTATAC 1 cut(s) 414
BsuRI GGCC 1 cut(s) 724
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 135, 208, 293
Cac8I GCNNGC 4 cut(s) 7, 107, 114, 582
CfoI GCGC 3 cut(s) 83, 232, 502
Cfr13I GGNCC 2 cut(s) 722, 723
Cfr9I CCCGGG 1 cut(s) 726
CseI GACGC 2 cut(s) 47, 54
Csp6I GTAC 2 cut(s) 186, 242
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CviAII CATG 7 cut(s) 122, 183, 274, 433, 443, 523, 635
CviQI GTAC 2 cut(s) 186, 242
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 402, 465, 600, 606
DpnII GATC 4 cut(s) 400, 463, 598, 604
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 2 cut(s) 11, 726
Eco31I GGTCTC 1 cut(s) 350
Eco47III AGCGCT 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 722, 723
EcoRII CCWGG 4 cut(s) 282, 318, 543, 667
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 2 cut(s) 11, 726
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 7 cut(s) 125, 186, 277, 436, 446, 526, 638
FaqI GGGAC 1 cut(s) 45
FatI CATG 7 cut(s) 121, 182, 273, 432, 442, 522, 634
FbaI TGATCA 1 cut(s) 604
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 110, 265, 665
FokI GGATG 3 cut(s) 142, 215, 300
FriOI GRGCYC 2 cut(s) 11, 726
Fsp4HI GCNGC 3 cut(s) 110, 265, 665
FspBI CTAG 2 cut(s) 239, 350
GlaI GCGC 3 cut(s) 82, 231, 501
GluI GCNGC 3 cut(s) 110, 265, 665
GsuI CTGGAG 1 cut(s) 527
HaeII RGCGCY 2 cut(s) 233, 503
HaeIII GGCC 1 cut(s) 724
HapII CCGG 2 cut(s) 29, 727
HgaI GACGC 2 cut(s) 47, 54
HhaI GCGC 3 cut(s) 83, 232, 502
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 7 cut(s) 125, 186, 277, 436, 446, 526, 638
Hin6I GCGC 3 cut(s) 81, 230, 500
HinP1I GCGC 3 cut(s) 81, 230, 500
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 2 cut(s) 176, 686
HinfI GANTC 1 cut(s) 33
HpaII CCGG 2 cut(s) 29, 727
Hpy166II GTNNAC 3 cut(s) 69, 414, 697
Hpy188I TCNGA 5 cut(s) 13, 311, 405, 468, 615
Hpy188III TCNNGA 4 cut(s) 209, 377, 507, 602
Hpy8I GTNNAC 3 cut(s) 69, 414, 697
Hpy99I CGWCG 2 cut(s) 41, 344
HpyAV CCTTC 1 cut(s) 547
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 20, 252, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 80, 102, 118
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 7 cut(s) 125, 186, 277, 436, 446, 526, 638
HspAI GCGC 3 cut(s) 81, 230, 500
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 4 cut(s) 400, 463, 598, 604
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 3 cut(s) 121, 251, 651
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 239, 350
MaeII ACGT 1 cut(s) 339
MalI GATC 4 cut(s) 402, 465, 600, 606
MbiI CCGCTC 1 cut(s) 74
MboI GATC 4 cut(s) 400, 463, 598, 604
MboII GAAGA 2 cut(s) 163, 407
MhlI GDGCHC 2 cut(s) 11, 726
MluCI AATT 2 cut(s) 305, 618
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 3 cut(s) 180, 334, 593
MseI TTAA 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 264, 737
MspI CCGG 2 cut(s) 29, 727
MspR9I CCNGG 7 cut(s) 30, 284, 320, 545, 669, 727, 728
MvaI CCWGG 4 cut(s) 284, 320, 545, 669
MwoI GCNNNNNNNGC 4 cut(s) 71, 80, 102, 118
NciI CCSGG 3 cut(s) 30, 727, 728
NdeII GATC 4 cut(s) 400, 463, 598, 604
NlaIII CATG 7 cut(s) 125, 186, 277, 436, 446, 526, 638
NlaIV GGNNCC 4 cut(s) 27, 195, 724, 725
NspI RCATGY 2 cut(s) 125, 277
PasI CCCWGGG 1 cut(s) 283
PciI ACATGT 1 cut(s) 273
PfoI TCCNGGA 1 cut(s) 543
PkrI GCNGC 3 cut(s) 111, 266, 666
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PscI ACATGT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 4 cut(s) 282, 318, 543, 667
PspGI CCWGG 4 cut(s) 282, 318, 543, 667
PspN4I GGNNCC 4 cut(s) 27, 195, 724, 725
PspOMI GGGCCC 1 cut(s) 722
PspPI GGNCC 2 cut(s) 722, 723
PvuII CAGCTG 2 cut(s) 264, 737
RsaI GTAC 2 cut(s) 187, 243
RsaNI GTAC 2 cut(s) 186, 242
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 3 cut(s) 110, 265, 665
Sau3AI GATC 4 cut(s) 400, 463, 598, 604
Sau96I GGNCC 2 cut(s) 722, 723
SchI GAGTC 1 cut(s) 27
ScrFI CCNGG 7 cut(s) 30, 284, 320, 545, 669, 727, 728
SduI GDGCHC 2 cut(s) 11, 726
SfaNI GCATC 1 cut(s) 469
SmaI CCCGGG 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 2 cut(s) 305, 618
SsiI CCGC 3 cut(s) 72, 94, 323
SspMI CTAG 2 cut(s) 239, 350
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 7 cut(s) 28, 282, 318, 543, 667, 725, 726
StyI CCWWGG 1 cut(s) 360
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 36, 508, 570
TaqII GACCGA 1 cut(s) 332
TasI AATT 2 cut(s) 305, 618
TatI WGTACW 2 cut(s) 185, 241
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TseI GCWGC 3 cut(s) 109, 264, 664
TspDTI ATGAA 6 cut(s) 170, 188, 431, 438, 488, 623
TspGWI ACGGA 1 cut(s) 179
TspMI CCCGGG 1 cut(s) 726
XceI RCATGY 2 cut(s) 125, 277
XmaI CCCGGG 1 cut(s) 726
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 2 cut(s) 239, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.