pycom08g03670

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029338
Physical Location & Seq
Forward (+)
2840208 .. 2842480
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03670.1

Sequence Viewer

Length: 1713 bp
ATGGTACGCAGCTATAGCAAGCAAAATCCGAATGGACTTAATCATGGCTACAGGAGAAAAGGTTTCTTCATAGTCAATCCCTTCTCTTTCAACGCCTATTTTCCTCTTGAAGATCCATTTGTTTCTAACAGGTACTATACCTTCTGGAGGGTCTACAAGAGTCCAGACTTGATTTTGATACATGGAATCCATCTCGGATTTCATAGGTCTTCCTTTGTGTCATTGTCACCTAGAAGGTGCAATTCCGCAAAGTCATTGTCTAAGCCATACCTTCTAGGTGGCTTATTGACCCGTTCAGATCTACGTGGAGCTAGGGGTTCAGGAACAGGGTTGTCAACATGTCGTGTGCTTGTTTGTGGTTCATCATTAATCTCATTAATTTCCATCGTTTTACTTATAGTTTCTTTGAGAACAAATTCGTCCTCTAGAAACTTAGCAGTTCTGGCGACAAAGACTTTCTGGTCGTCAGGATGTAGCTTCAAGCTTCTTGACATATGCTTCACAACCCCAAATCTTAATGTGATTAAGACTTGGCTTTCTACCATGCCATATCTCATAGGGCGTTTCATATCCCCAGAATGTTACTGGCAGATCAGCGGAACTCATCATAGAACGAACCATGTTCATCAAGGTTCGATTTCTCCTTTCATCAAGGTTCGATTTGCAAAAATGTGGATTCTCGCAGCCGAGTTTGAGATTCGACAACTGAAACTCGAGTCTGCACGTAAGATACTTGGTACTGCAATTGGCAAGGCAGCTAAAGGCAAGATATTTAAGAAGTACATTGAGATTGAGTGGCAACTATGTAATGCTGATCGCTGTCGCAAACTGTATGAAAAGTATGCAGAGTTTGAGAAAAGAATTTGCGACACAGAAAGAGCCAGATCGATTTATGAACTTGCCATCGGCCAAAAACAACTCGACAAGCCCGAATTGTTGTGGAAGTCATACATTGACTTTGAACTAGCAGAGAGAGAGTTTGAGAGAGCTAGAAATCTGTACAGGCGCCTATTAGAACGGACCCGACACGTTAAAGCATCGGCTGTGGTGGGAGGAGACGGAATGCGCTCAGAAGATGTTATTGAGCAAAAGGAACAATGCATTCAGCGCGCCAGAAGGGTTTTCGAGAGAGCGTCAGACTATTTTAGGACTTCAGCACCGGAACTTAAGGAACAAAGAAGTATGCTGCTAGAACAATGGCTGAACATGGAGAAGGAGTTTGGGGATTTCGATGATGTTACGTTGGTGCAATTATTGAAGATGGTGTTTCTGCTGGGTTTGAAGAATACATTGATCATCTTTTTCCAGAATAAGCTCTTAACACGAACTGCAAGCTCTTCGAAGCTGCCTACAACTGGAAGAGGCGAAAAGATTCTTCTGCGGAGGATGGGGACGATAAAGAAACATAGTACATTGTATGTTTCGGGTTATCATGAGAATCGTTTGGAAAAAAAAATATTTGAGTGGTTTTTAAAGTTTTTATTAAGAGTATTGTTATTCATAGTTTCATATTATGTTTTTATTCCAAATTTTTGTACTATCTTAGATTTTAGTTTATTATTTAATAAATTAATAATTAAAAAAATTAATTTTAAAAAAAAACAGTTCGTTTGGAAGTATTTTAAAATATATTTTCATGTAAATATTTTGAAATCAATTCTTAGTAAAAATAGAAAAAACATATCGATGATATTCCTTATAGAAAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

571

Amino Acids

66.63

Weight (kDa)

9.99

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029338)

Species Orthologous Gene IDs
pyrus_communis pycom08g03670 pycom08g03770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 1005
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 1110
AciI CCGC 3 cut(s) 246, 597, 1381
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 3 cut(s) 415, 861, 1528
AcuI CTGAAG 1 cut(s) 1137
AcyI GRCGYC 1 cut(s) 1006
AfaI GTAC 7 cut(s) 6, 134, 739, 782, 1001, 1411, 1537
AfiI CCNNNNNNNGG 2 cut(s) 147, 1355
AflII CTTAAG 1 cut(s) 1166
AflIII ACRYGT 2 cut(s) 338, 1027
AgsI TTSAA 7 cut(s) 91, 110, 481, 962, 1258, 1282, 1651
AluBI AGCT 9 cut(s) 12, 311, 477, 484, 758, 989, 1315, 1335, 1345
AluI AGCT 9 cut(s) 12, 311, 477, 484, 758, 989, 1315, 1335, 1345
Alw26I GTCTC 1 cut(s) 1050
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 1 cut(s) 907
ApeKI GCWGC 5 cut(s) 9, 683, 755, 1186, 1345
ApoI RAATTY 3 cut(s) 415, 861, 1528
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 4 cut(s) 368, 377, 1571, 1587
Asp700I GAANNNNTTC 2 cut(s) 415, 1371
AspLEI GCGC 4 cut(s) 1008, 1068, 1110, 1112
AspS9I GGNCC 1 cut(s) 1020
AsuHPI GGTGA 1 cut(s) 219
AsuII TTCGAA 1 cut(s) 1340
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 1 cut(s) 1020
BanI GGYRCC 1 cut(s) 1005
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 5 cut(s) 21, 695, 767, 1173, 1332
BccI CCATC 5 cut(s) 198, 392, 911, 1255, 1381
BcgI CGANNNNNNTGC 2 cut(s) 1320, 1354
BclI TGATCA 1 cut(s) 1293
BcoDI GTCTC 1 cut(s) 1050
BfaI CTAG 7 cut(s) 231, 275, 312, 426, 965, 990, 1190
BfmI CTRYAG 2 cut(s) 13, 49
BfoI RGCGCY 1 cut(s) 1009
BfrI CTTAAG 1 cut(s) 1166
BglII AGATCT 1 cut(s) 298
BisI GCNGC 5 cut(s) 10, 684, 756, 1187, 1346
BlsI GCNGC 5 cut(s) 11, 685, 757, 1188, 1347
Bme18I GGWCC 1 cut(s) 1020
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 1020
BmiI GGNNCC 2 cut(s) 1007, 1022
BmsI GCATC 1 cut(s) 1046
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 166
Bpu14I TTCGAA 1 cut(s) 1340
Bsa29I ATCGAT 2 cut(s) 887, 1685
BsaAI YACGTR 2 cut(s) 305, 725
BsaHI GRCGYC 1 cut(s) 1006
BsaWI WCCGGW 1 cut(s) 1159
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 1202, 1232
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 1355
Bse1I ACTGG 2 cut(s) 590, 1360
BseCI ATCGAT 2 cut(s) 887, 1685
BseGI GGATG 2 cut(s) 476, 1392
BseLI CCNNNNNNNGG 2 cut(s) 147, 1355
BseMII CTCAG 1 cut(s) 1083
BseNI ACTGG 2 cut(s) 590, 1360
BsePI GCGCGC 1 cut(s) 1108
BseRI GAGGAG 1 cut(s) 1068
BseXI GCAGC 5 cut(s) 21, 695, 767, 1173, 1332
BseYI CCCAGC 1 cut(s) 1273
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 1 cut(s) 1110
BshFI GGCC 1 cut(s) 909
BshNI GGYRCC 1 cut(s) 1005
BshVI ATCGAT 2 cut(s) 887, 1685
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 1 cut(s) 1160
BslFI GGGAC 1 cut(s) 1405
BslI CCNNNNNNNGG 2 cut(s) 147, 1355
BsmAI GTCTC 1 cut(s) 1050
BsmBI CGTCTC 1 cut(s) 1050
BsmFI GGGAC 1 cut(s) 1405
BsmI GAATGC 2 cut(s) 1068, 1101
BsnI GGCC 1 cut(s) 909
BsoBI CYCGRG 1 cut(s) 713
Bsp119I TTCGAA 1 cut(s) 1340
Bsp1407I TGTACA 1 cut(s) 999
Bsp143I GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
BspACI CCGC 3 cut(s) 246, 597, 1381
BspANI GGCC 1 cut(s) 909
BspCNI CTCAG 1 cut(s) 1082
BspDI ATCGAT 2 cut(s) 887, 1685
BspFNI CGCG 1 cut(s) 1110
BspHI TCATGA 1 cut(s) 1432
BspLI GGNNCC 2 cut(s) 1007, 1022
BspPI GGATC 1 cut(s) 107
BspQI GCTCTTC 1 cut(s) 1342
BspT104I TTCGAA 1 cut(s) 1340
BspT107I GGYRCC 1 cut(s) 1005
BspTI CTTAAG 1 cut(s) 1166
BsrGI TGTACA 1 cut(s) 999
BsrI ACTGG 2 cut(s) 590, 1360
BssHII GCGCGC 1 cut(s) 1108
BssMI GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
BssNI GRCGYC 1 cut(s) 1006
Bst4CI ACNGT 2 cut(s) 831, 1605
Bst6I CTCTTC 2 cut(s) 1342, 1354
BstACI GRCGYC 1 cut(s) 1006
BstAFI CTTAAG 1 cut(s) 1166
BstAUI TGTACA 1 cut(s) 999
BstBAI YACGTR 2 cut(s) 305, 725
BstBI TTCGAA 1 cut(s) 1340
BstC8I GCNNGC 3 cut(s) 20, 1110, 1333
BstDEI CTNAG 5 cut(s) 261, 433, 1069, 1543, 1661
BstENI CCTNNNNNAGG 1 cut(s) 145
BstF5I GGATG 2 cut(s) 476, 1392
BstFNI CGCG 1 cut(s) 1110
BstH2I RGCGCY 1 cut(s) 1009
BstHHI GCGC 4 cut(s) 1008, 1068, 1110, 1112
BstKTI GATC 6 cut(s) 115, 301, 594, 817, 887, 1296
BstMAI GTCTC 1 cut(s) 1050
BstMBI GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
BstMWI GCNNNNNNNGC 3 cut(s) 15, 443, 1107
BstNSI RCATGY 1 cut(s) 342
BstSFI CTRYAG 2 cut(s) 13, 49
BstUI CGCG 1 cut(s) 1110
BstV1I GCAGC 5 cut(s) 21, 695, 767, 1173, 1332
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 112, 298
BstYI RGATCY 2 cut(s) 112, 298
Bsu15I ATCGAT 2 cut(s) 887, 1685
BsuRI GGCC 1 cut(s) 909
BsuTUI ATCGAT 2 cut(s) 887, 1685
BtsCI GGATG 2 cut(s) 476, 1392
Cac8I GCNNGC 3 cut(s) 20, 1110, 1333
CciI TCATGA 1 cut(s) 1432
CfoI GCGC 4 cut(s) 1008, 1068, 1110, 1112
Cfr13I GGNCC 1 cut(s) 1020
ClaI ATCGAT 2 cut(s) 887, 1685
CseI GACGC 1 cut(s) 1122
Csp6I GTAC 7 cut(s) 5, 133, 738, 781, 1000, 1410, 1536
CviAII CATG 8 cut(s) 44, 182, 339, 544, 620, 1207, 1433, 1637
CviQI GTAC 7 cut(s) 5, 133, 738, 781, 1000, 1410, 1536
DdeI CTNAG 5 cut(s) 261, 433, 1069, 1543, 1661
DinI GGCGCC 1 cut(s) 1007
DpnI GATC 6 cut(s) 114, 300, 593, 816, 886, 1295
DpnII GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
DraI TTTAAA 3 cut(s) 1473, 1594, 1624
DrdI GACNNNNNNGTC 1 cut(s) 460
DseDI GACNNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 907
Eam1104I CTCTTC 2 cut(s) 1342, 1354
EarI CTCTTC 2 cut(s) 1342, 1354
Eco47I GGWCC 1 cut(s) 1020
Eco57I CTGAAG 1 cut(s) 1137
Eco88I CYCGRG 1 cut(s) 713
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 1103
EgeI GGCGCC 1 cut(s) 1007
EheI GGCGCC 1 cut(s) 1007
Esp3I CGTCTC 1 cut(s) 1050
FaeI CATG 8 cut(s) 47, 185, 342, 547, 623, 1210, 1436, 1640
FaqI GGGAC 1 cut(s) 1405
FatI CATG 8 cut(s) 43, 181, 338, 543, 619, 1206, 1432, 1636
FauNDI CATATG 1 cut(s) 494
FbaI TGATCA 1 cut(s) 1293
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 5 cut(s) 10, 684, 756, 1187, 1346
FokI GGATG 2 cut(s) 483, 1399
Fsp4HI GCNGC 5 cut(s) 10, 684, 756, 1187, 1346
FspBI CTAG 7 cut(s) 231, 275, 312, 426, 965, 990, 1190
GlaI GCGC 4 cut(s) 1007, 1067, 1109, 1111
GluI GCNGC 5 cut(s) 10, 684, 756, 1187, 1346
GsaI CCCAGC 1 cut(s) 1277
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 1009
HaeIII GGCC 1 cut(s) 909
HapII CCGG 1 cut(s) 1160
HgaI GACGC 1 cut(s) 1122
HhaI GCGC 4 cut(s) 1008, 1068, 1110, 1112
Hin1I GRCGYC 1 cut(s) 1006
Hin1II CATG 8 cut(s) 47, 185, 342, 547, 623, 1210, 1436, 1640
Hin6I GCGC 4 cut(s) 1006, 1066, 1108, 1110
HinP1I GCGC 4 cut(s) 1006, 1066, 1108, 1110
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 7 cut(s) 160, 186, 676, 697, 716, 1372, 1438
HpaII CCGG 1 cut(s) 1160
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 2 cut(s) 154, 336
Hpy188I TCNGA 5 cut(s) 30, 197, 298, 1072, 1138
Hpy8I GTNNAC 2 cut(s) 154, 336
HpyAV CCTTC 6 cut(s) 91, 151, 228, 281, 1110, 1207
HpyCH4III ACNGT 2 cut(s) 831, 1605
HpyCH4IV ACGT 4 cut(s) 304, 724, 1029, 1241
HpyCH4V TGCA 8 cut(s) 240, 665, 722, 743, 845, 1101, 1249, 1331
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 443, 1107
HpyF3I CTNAG 5 cut(s) 261, 433, 1069, 1543, 1661
HpySE526I ACGT 4 cut(s) 304, 724, 1029, 1241
Hsp92I GRCGYC 1 cut(s) 1006
Hsp92II CATG 8 cut(s) 47, 185, 342, 547, 623, 1210, 1436, 1640
HspAI GCGC 4 cut(s) 1006, 1066, 1108, 1110
KasI GGCGCC 1 cut(s) 1005
Ksp22I TGATCA 1 cut(s) 1293
Kzo9I GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
LguI GCTCTTC 1 cut(s) 1342
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 5 cut(s) 21, 695, 767, 1173, 1332
LweI GCATC 1 cut(s) 1046
MaeI CTAG 7 cut(s) 231, 275, 312, 426, 965, 990, 1190
MaeII ACGT 4 cut(s) 304, 724, 1029, 1241
MaeIII GTNAC 3 cut(s) 225, 581, 1237
MalI GATC 6 cut(s) 114, 300, 593, 816, 886, 1295
MboI GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
MboII GAAGA 9 cut(s) 58, 122, 201, 1085, 1270, 1294, 1329, 1367, 1371
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 2 cut(s) 112, 298
Mly113I GGCGCC 1 cut(s) 1006
MlyI GAGTC 2 cut(s) 169, 725
MnlI CCTC 6 cut(s) 114, 141, 433, 1046, 1355, 1377
Mph1103I ATGCAT 1 cut(s) 1103
MroXI GAANNNNTTC 2 cut(s) 415, 1371
MslI CAYNNNNRTG 1 cut(s) 1685
MspA1I CMGCKG 1 cut(s) 597
MspCI CTTAAG 1 cut(s) 1166
MspI CCGG 1 cut(s) 1160
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 1068, 1101
MvnI CGCG 1 cut(s) 1110
MwoI GCNNNNNNNGC 3 cut(s) 15, 443, 1107
NarI GGCGCC 1 cut(s) 1006
NdeI CATATG 1 cut(s) 494
NdeII GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
NlaIII CATG 8 cut(s) 47, 185, 342, 547, 623, 1210, 1436, 1640
NlaIV GGNNCC 2 cut(s) 1007, 1022
NmeAIII GCCGAG 1 cut(s) 712
NmuCI GTSAC 1 cut(s) 225
NsiI ATGCAT 1 cut(s) 1103
NspI RCATGY 1 cut(s) 342
NspV TTCGAA 1 cut(s) 1340
PaeR7I CTCGAG 1 cut(s) 713
PagI TCATGA 1 cut(s) 1432
PauI GCGCGC 1 cut(s) 1108
PciI ACATGT 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PctI GAATGC 2 cut(s) 1068, 1101
PdmI GAANNNNTTC 2 cut(s) 415, 1371
PfeI GAWTC 5 cut(s) 186, 676, 697, 1372, 1438
PkrI GCNGC 5 cut(s) 11, 685, 757, 1188, 1347
PleI GAGTC 2 cut(s) 168, 724
PluTI GGCGCC 1 cut(s) 1009
PpsI GAGTC 2 cut(s) 168, 724
Ppu21I YACGTR 2 cut(s) 305, 725
PscI ACATGT 1 cut(s) 338
PshBI ATTAAT 4 cut(s) 368, 377, 1571, 1587
PspFI CCCAGC 1 cut(s) 1273
PspN4I GGNNCC 2 cut(s) 1007, 1022
PspPI GGNCC 1 cut(s) 1020
PspXI VCTCGAGB 1 cut(s) 713
PsuI RGATCY 2 cut(s) 112, 298
PteI GCGCGC 1 cut(s) 1108
RsaI GTAC 7 cut(s) 6, 134, 739, 782, 1001, 1411, 1537
RsaNI GTAC 7 cut(s) 5, 133, 738, 781, 1000, 1410, 1536
RseI CAYNNNNRTG 1 cut(s) 1685
SapI GCTCTTC 1 cut(s) 1342
SatI GCNGC 5 cut(s) 10, 684, 756, 1187, 1346
Sau3AI GATC 6 cut(s) 112, 298, 591, 814, 884, 1293
Sau96I GGNCC 1 cut(s) 1020
SchI GAGTC 2 cut(s) 169, 725
SfaNI GCATC 1 cut(s) 1046
SfcI CTRYAG 2 cut(s) 13, 49
SfoI GGCGCC 1 cut(s) 1007
Sfr274I CTCGAG 1 cut(s) 713
SfuI TTCGAA 1 cut(s) 1340
SinI GGWCC 1 cut(s) 1020
SlaI CTCGAG 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 1685
SmlI CTYRAG 2 cut(s) 713, 1166
SmoI CTYRAG 2 cut(s) 713, 1166
SsiI CCGC 3 cut(s) 246, 597, 1381
SspDI GGCGCC 1 cut(s) 1005
SspI AATATT 2 cut(s) 1458, 1645
SspMI CTAG 7 cut(s) 231, 275, 312, 426, 965, 990, 1190
TaaI ACNGT 2 cut(s) 831, 1605
TaiI ACGT 4 cut(s) 307, 727, 1032, 1244
TatI WGTACW 4 cut(s) 780, 999, 1409, 1535
TfiI GAWTC 5 cut(s) 186, 676, 697, 1372, 1438
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 5 cut(s) 9, 683, 755, 1186, 1345
Tsp45I GTSAC 1 cut(s) 225
TspGWI ACGGA 2 cut(s) 1033, 1074
Vha464I CTTAAG 1 cut(s) 1166
VpaK11BI GGWCC 1 cut(s) 1020
VspI ATTAAT 4 cut(s) 368, 377, 1571, 1587
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 3 cut(s) 415, 861, 1528
XbaI TCTAGA 1 cut(s) 425
XceI RCATGY 1 cut(s) 342
XcmI CCANNNNNNNNNTGG 1 cut(s) 582
XhoI CTCGAG 1 cut(s) 713
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 2 cut(s) 415, 1371
XspI CTAG 7 cut(s) 231, 275, 312, 426, 965, 990, 1190
Zsp2I ATGCAT 1 cut(s) 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.