pycom08g03770

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029338
Physical Location & Seq
Forward (+)
2886036 .. 2887363
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03770.1

Sequence Viewer

Length: 1221 bp
ATGTGCCTTGAACTGATTCCTCATAAGAAGTTTACATTTGCAAAAATGTGGATTCTCGCAGCCGAGTTTGAGATCCGACAACTAAAACTCGAGTCTGCATGTAAGATACTTGGTACTGCAATTGGCAAGGCAGCTAAAGGCAAGATATTTAAGAGGTACATTGAGATTGAGTGGCAACTATGTAATGCTGATCGCTGTCGGAAACTGTATGAAAAGTATCTGCATTGGTCGCCAGAAAATTGCTACGCGTGGATCAGGTATGCAGAGTTTGAGAAAAAAATTTGCGACACAGAACGAGCCAGATCAGTTTATGAACTTGCCATCGGGCAAAAACAACTCGACAAGCCCGAATTGTTGTGGAAGTCATACATTGACTTTGAACTAGCAGAGAGAGAGTTTGAGAGAGCTAGAAATCTGTACAGGCGACTCTTAGAACGGACCCAACACGTTAAAGTATGGATCAGTTACGCAACATTCAAGGCATCGGCTATGGTGGGAGGAGATGAAATGCGCTCAGGAGATGTTATTGAGCAAAAGGAACAATGCATTCAGCGCGCCAGAAGGGTTTTCGAGAGAGCATCGGACTATTTTAGGACTTCAGCACCGGAACTTAAGGAACAAAGAAGTACGCTGCTAGAACAATGGCTGAACATGGAGAAGGAGTTTGGGGATTTCGGTGATGTTACGTTGGTGCAAGCGAAGCTGCCGCGGAGATTGAAGAGGAAGAGGCCAATAGTTATTGAAGATGGTGGTTCTGCTGGGTTTGAAGAATATATTGATTATCTTTTTCCAGAAGAAGCTCTTAACACGAACTGCAAGCTCTTCGAAGCTGCCTACAATTGGAAGAGGCGAAAAGATTCTTCTGCAGAGGATGGCGAAGATAAAGAAACATATAAAAGAGTCACGTGCTTTGATACAGCGTTGGGCATGGCCAAGTTAACTAAAATTGTAACTCTATCTGTAACTAACTTGTTATATGATGAAAATTTAACTTCATTCATTAAGAAAAAGAGCACAGGTCCAATGCCGTCAGCTTCAATCCATTTCATGCCACCGTCAATCCATTTCATGACAGTCAATGCCGTCAGCTTCTCTGTTGACATGATGATTGTATCTGACCTGTTGAAAAATCACCATGAAAGAGCACAGGTCCAAGCAGCGAGGAGTCAGCAGAGGAAGGCTTTGAGGAATCATTTTCCAATGCCTGCCAAGTTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

407

Amino Acids

47.52

Weight (kDa)

9.11

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_Syf1_CNRKL1_C PF23231 41 - 138 3.4e-06 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 50 - 145 2.6e-11 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 111 - 219 1.7e-08 PRP39 C-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029338)

Species Orthologous Gene IDs
pyrus_communis pycom08g03670 pycom08g03770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 248, 555, 709
AciI CCGC 2 cut(s) 707, 709
AclWI GGATC 3 cut(s) 67, 260, 467
AcoI YGGCCR 1 cut(s) 930
AcsI RAATTY 2 cut(s) 279, 985
AcuI CTGAAG 1 cut(s) 582
AcvI CACGTG 1 cut(s) 906
AfaI GTAC 4 cut(s) 115, 158, 419, 628
AfiI CCNNNNNNNGG 1 cut(s) 840
AflII CTTAAG 1 cut(s) 611
AflIII ACRYGT 2 cut(s) 246, 445
AgsI TTSAA 8 cut(s) 11, 380, 478, 718, 743, 767, 1038, 1126
AluBI AGCT 8 cut(s) 134, 407, 703, 800, 820, 830, 1034, 1089
AluI AGCT 8 cut(s) 134, 407, 703, 800, 820, 830, 1034, 1089
Alw21I GWGCWC 2 cut(s) 1016, 1147
AlwI GGATC 3 cut(s) 67, 260, 467
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 2 cut(s) 728, 930
ApeKI GCWGC 6 cut(s) 59, 131, 631, 703, 830, 1157
ApoI RAATTY 2 cut(s) 279, 985
Asp700I GAANNNNTTC 2 cut(s) 15, 856
AspLEI GCGC 3 cut(s) 513, 555, 557
AspS9I GGNCC 3 cut(s) 438, 1019, 1150
AsuHPI GGTGA 2 cut(s) 689, 1124
AsuII TTCGAA 1 cut(s) 825
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 3 cut(s) 438, 1019, 1150
BalI TGGCCA 1 cut(s) 932
BbrPI CACGTG 1 cut(s) 906
Bbv12I GWGCWC 2 cut(s) 1016, 1147
BbvI GCAGC 6 cut(s) 71, 143, 618, 690, 817, 1169
BccI CCATC 3 cut(s) 329, 740, 866
BceAI ACGGC 2 cut(s) 1012, 1067
BcgI CGANNNNNNTGC 2 cut(s) 805, 839
BfaI CTAG 3 cut(s) 383, 408, 635
BfmI CTRYAG 1 cut(s) 864
BfrI CTTAAG 1 cut(s) 611
BisI GCNGC 7 cut(s) 60, 132, 632, 704, 707, 831, 1158
BlsI GCNGC 7 cut(s) 61, 133, 633, 705, 708, 832, 1159
Bme18I GGWCC 3 cut(s) 438, 1019, 1150
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 438, 1019, 1150
BmiI GGNNCC 1 cut(s) 440
BmsI GCATC 2 cut(s) 491, 587
Bpu10I CCTNAGC 1 cut(s) 514
Bpu14I TTCGAA 1 cut(s) 825
BsaAI YACGTR 1 cut(s) 906
BsaJI CCNNGG 1 cut(s) 707
BsaWI WCCGGW 1 cut(s) 604
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 840
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 1 cut(s) 840
BseMII CTCAG 1 cut(s) 528
BsePI GCGCGC 1 cut(s) 553
BseRI GAGGAG 2 cut(s) 513, 1177
BseXI GCAGC 6 cut(s) 71, 143, 618, 690, 817, 1169
BseYI CCCAGC 1 cut(s) 758
Bsh1236I CGCG 3 cut(s) 248, 555, 709
BshFI GGCC 2 cut(s) 730, 932
BsiHKAI GWGCWC 2 cut(s) 1016, 1147
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 605
BslI CCNNNNNNNGG 1 cut(s) 840
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 2 cut(s) 730, 932
BsoBI CYCGRG 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 2 cut(s) 1016, 1147
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 5 cut(s) 72, 190, 252, 302, 459
BspACI CCGC 2 cut(s) 707, 709
BspANI GGCC 2 cut(s) 730, 932
BspCNI CTCAG 1 cut(s) 527
BspFNI CGCG 3 cut(s) 248, 555, 709
BspHI TCATGA 2 cut(s) 1068, 1217
BspLI GGNNCC 1 cut(s) 440
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 3 cut(s) 67, 260, 467
BspQI GCTCTTC 1 cut(s) 827
BspT104I TTCGAA 1 cut(s) 825
BspTI CTTAAG 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 417
BssECI CCNNGG 1 cut(s) 707
BssHII GCGCGC 1 cut(s) 553
BssMI GATC 5 cut(s) 72, 190, 252, 302, 459
Bst4CI ACNGT 3 cut(s) 207, 1056, 1075
Bst6I CTCTTC 4 cut(s) 713, 719, 827, 839
BstAFI CTTAAG 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 906
BstBI TTCGAA 1 cut(s) 825
BstC8I GCNNGC 4 cut(s) 555, 696, 818, 1206
BstDEI CTNAG 2 cut(s) 430, 514
BstDSI CCRYGG 1 cut(s) 707
BstF5I GGATG 1 cut(s) 877
BstFNI CGCG 3 cut(s) 248, 555, 709
BstHHI GCGC 3 cut(s) 513, 555, 557
BstKTI GATC 5 cut(s) 75, 193, 255, 305, 462
BstMBI GATC 5 cut(s) 72, 190, 252, 302, 459
BstMWI GCNNNNNNNGC 3 cut(s) 229, 552, 700
BstNSI RCATGY 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 3 cut(s) 248, 555, 709
BstV1I GCAGC 6 cut(s) 71, 143, 618, 690, 817, 1169
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BsuRI GGCC 2 cut(s) 730, 932
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 1 cut(s) 877
Cac8I GCNNGC 4 cut(s) 555, 696, 818, 1206
CciI TCATGA 2 cut(s) 1068, 1217
CfoI GCGC 3 cut(s) 513, 555, 557
Cfr13I GGNCC 3 cut(s) 438, 1019, 1150
Cfr42I CCGCGG 1 cut(s) 710
Csp6I GTAC 4 cut(s) 114, 157, 418, 627
CviAII CATG 8 cut(s) 99, 652, 928, 1048, 1069, 1102, 1136, 1218
CviQI GTAC 4 cut(s) 114, 157, 418, 627
DdeI CTNAG 2 cut(s) 430, 514
DpnI GATC 5 cut(s) 74, 192, 254, 304, 461
DpnII GATC 5 cut(s) 72, 190, 252, 302, 459
EaeI YGGCCR 1 cut(s) 930
Eam1104I CTCTTC 4 cut(s) 713, 719, 827, 839
EarI CTCTTC 4 cut(s) 713, 719, 827, 839
Eco47I GGWCC 3 cut(s) 438, 1019, 1150
Eco57I CTGAAG 1 cut(s) 582
Eco72I CACGTG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 548
FaeI CATG 8 cut(s) 102, 655, 931, 1051, 1072, 1105, 1139, 1221
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FatI CATG 8 cut(s) 98, 651, 927, 1047, 1068, 1101, 1135, 1217
Fnu4HI GCNGC 7 cut(s) 60, 132, 632, 704, 707, 831, 1158
FokI GGATG 1 cut(s) 884
Fsp4HI GCNGC 7 cut(s) 60, 132, 632, 704, 707, 831, 1158
FspBI CTAG 3 cut(s) 383, 408, 635
GlaI GCGC 3 cut(s) 512, 554, 556
GluI GCNGC 7 cut(s) 60, 132, 632, 704, 707, 831, 1158
GsaI CCCAGC 1 cut(s) 762
HaeIII GGCC 2 cut(s) 730, 932
HapII CCGG 1 cut(s) 605
HhaI GCGC 3 cut(s) 513, 555, 557
Hin1II CATG 8 cut(s) 102, 655, 931, 1051, 1072, 1105, 1139, 1221
Hin6I GCGC 3 cut(s) 511, 553, 555
HinP1I GCGC 3 cut(s) 511, 553, 555
HincII GTYRAC 2 cut(s) 939, 1099
HindII GTYRAC 2 cut(s) 939, 1099
HinfI GANTC 8 cut(s) 16, 52, 92, 426, 857, 900, 1165, 1189
HpaI GTTAAC 1 cut(s) 939
HpaII CCGG 1 cut(s) 605
HphI GGTGA 2 cut(s) 689, 1124
Hpy166II GTNNAC 3 cut(s) 33, 939, 1099
Hpy188I TCNGA 4 cut(s) 77, 201, 583, 1117
Hpy188III TCNNGA 5 cut(s) 516, 571, 791, 1069, 1218
Hpy8I GTNNAC 3 cut(s) 33, 939, 1099
HpyAV CCTTC 3 cut(s) 555, 652, 1171
HpyCH4III ACNGT 3 cut(s) 207, 1056, 1075
HpyCH4IV ACGT 3 cut(s) 447, 686, 905
HpyCH4V TGCA 9 cut(s) 41, 98, 119, 223, 263, 546, 694, 816, 866
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 552, 700
HpyF3I CTNAG 2 cut(s) 430, 514
HpySE526I ACGT 3 cut(s) 447, 686, 905
Hsp92II CATG 8 cut(s) 102, 655, 931, 1051, 1072, 1105, 1139, 1221
HspAI GCGC 3 cut(s) 511, 553, 555
KspAI GTTAAC 1 cut(s) 939
KspI CCGCGG 1 cut(s) 710
Kzo9I GATC 5 cut(s) 72, 190, 252, 302, 459
LguI GCTCTTC 1 cut(s) 827
Lsp1109I GCAGC 6 cut(s) 71, 143, 618, 690, 817, 1169
LweI GCATC 2 cut(s) 491, 587
MaeI CTAG 3 cut(s) 383, 408, 635
MaeII ACGT 3 cut(s) 447, 686, 905
MaeIII GTNAC 5 cut(s) 464, 682, 901, 949, 961
MalI GATC 5 cut(s) 74, 192, 254, 304, 461
MboI GATC 5 cut(s) 72, 190, 252, 302, 459
MboII GAAGA 9 cut(s) 730, 736, 755, 779, 806, 814, 852, 856, 890
MfeI CAATTG 2 cut(s) 120, 838
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 1016, 1147
MlsI TGGCCA 1 cut(s) 932
MluCI AATT 7 cut(s) 120, 238, 279, 350, 838, 945, 985
MluI ACGCGT 1 cut(s) 246
MluNI TGGCCA 1 cut(s) 932
MlyI GAGTC 4 cut(s) 101, 420, 909, 1174
MmeI TCCRAC 2 cut(s) 100, 179
Mox20I TGGCCA 1 cut(s) 932
Mph1103I ATGCAT 1 cut(s) 548
MroXI GAANNNNTTC 2 cut(s) 15, 856
MscI TGGCCA 1 cut(s) 932
MseI TTAA 7 cut(s) 150, 450, 612, 804, 938, 989, 1002
Msp20I TGGCCA 1 cut(s) 932
MspA1I CMGCKG 1 cut(s) 709
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 1 cut(s) 605
MunI CAATTG 2 cut(s) 120, 838
Mva1269I GAATGC 1 cut(s) 546
MvnI CGCG 3 cut(s) 248, 555, 709
MwoI GCNNNNNNNGC 3 cut(s) 229, 552, 700
NdeII GATC 5 cut(s) 72, 190, 252, 302, 459
NlaIII CATG 8 cut(s) 102, 655, 931, 1051, 1072, 1105, 1139, 1221
NlaIV GGNNCC 1 cut(s) 440
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 901
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 1 cut(s) 102
NspV TTCGAA 1 cut(s) 825
PaeR7I CTCGAG 1 cut(s) 89
PagI TCATGA 2 cut(s) 1068, 1217
PauI GCGCGC 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 827
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 546
PdmI GAANNNNTTC 2 cut(s) 15, 856
PfeI GAWTC 4 cut(s) 16, 52, 857, 1189
PkrI GCNGC 7 cut(s) 61, 133, 633, 705, 708, 832, 1159
PleI GAGTC 4 cut(s) 100, 420, 908, 1173
PmaCI CACGTG 1 cut(s) 906
PmlI CACGTG 1 cut(s) 906
PpsI GAGTC 4 cut(s) 100, 420, 908, 1173
Ppu21I YACGTR 1 cut(s) 906
PspCI CACGTG 1 cut(s) 906
PspFI CCCAGC 1 cut(s) 758
PspN4I GGNNCC 1 cut(s) 440
PspPI GGNCC 3 cut(s) 438, 1019, 1150
PspXI VCTCGAGB 1 cut(s) 89
PstI CTGCAG 1 cut(s) 868
PsuI RGATCY 1 cut(s) 72
PteI GCGCGC 1 cut(s) 553
RsaI GTAC 4 cut(s) 115, 158, 419, 628
RsaNI GTAC 4 cut(s) 114, 157, 418, 627
SacII CCGCGG 1 cut(s) 710
SapI GCTCTTC 1 cut(s) 827
SaqAI TTAA 7 cut(s) 150, 450, 612, 804, 938, 989, 1002
SatI GCNGC 7 cut(s) 60, 132, 632, 704, 707, 831, 1158
Sau3AI GATC 5 cut(s) 72, 190, 252, 302, 459
Sau96I GGNCC 3 cut(s) 438, 1019, 1150
SchI GAGTC 4 cut(s) 101, 420, 909, 1174
SduI GDGCHC 2 cut(s) 1016, 1147
SfaNI GCATC 2 cut(s) 491, 587
SfcI CTRYAG 1 cut(s) 864
Sfr274I CTCGAG 1 cut(s) 89
Sfr303I CCGCGG 1 cut(s) 710
SfuI TTCGAA 1 cut(s) 825
SgrBI CCGCGG 1 cut(s) 710
SinI GGWCC 3 cut(s) 438, 1019, 1150
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 89, 611
SmoI CTYRAG 2 cut(s) 89, 611
Sse9I AATT 7 cut(s) 120, 238, 279, 350, 838, 945, 985
SsiI CCGC 2 cut(s) 707, 709
SspMI CTAG 3 cut(s) 383, 408, 635
TaaI ACNGT 3 cut(s) 207, 1056, 1075
TaiI ACGT 3 cut(s) 450, 689, 908
TaqI TCGA 4 cut(s) 90, 339, 570, 825
TasI AATT 7 cut(s) 120, 238, 279, 350, 838, 945, 985
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 709
TfiI GAWTC 4 cut(s) 16, 52, 857, 1189
Tru1I TTAA 7 cut(s) 150, 450, 612, 804, 938, 989, 1002
Tru9I TTAA 7 cut(s) 150, 450, 612, 804, 938, 989, 1002
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 6 cut(s) 59, 131, 631, 703, 830, 1157
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 9 cut(s) 225, 327, 519, 984, 988, 996, 1036, 1057, 1152
TspGWI ACGGA 1 cut(s) 451
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 3 cut(s) 438, 1019, 1150
XapI RAATTY 2 cut(s) 279, 985
XceI RCATGY 1 cut(s) 102
XhoI CTCGAG 1 cut(s) 89
XmnI GAANNNNTTC 2 cut(s) 15, 856
XspI CTAG 3 cut(s) 383, 408, 635
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.