pycom08g16140

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0021880
Physical Location & Seq
Reverse (-)
16018886 .. 16023457
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
ATGCAGAGGAAGAGCTGGAGAACATTGGCGACAGTGAGGAAGGTGCTGACGTGCGCCGTATGCTTAATTGCGCTAGTGGCTCTCTTCTCTTTCTCCAAAGTCCCTAACTTCTCCGACCCCGTCAAGCTTCCCACGTTGGATGATACAAGTCGGATATTGGATGACAAGCTATGGAAGATTCCACCAACTCCTGATTTTGTGCAGTGTGTAGAGCCAAGTCCGAACTATACACCTCCTGCAGAGTCACGGGGCTACTTTCAAGTGCGTACAAATGGTGGTCTCAACCAGATGCGTGCAGGGATATGTGACATGGTAGCTGTAGCCCGCATCATAAATGCCACTCTTGTTATTCCAGAACTTGATCAACGGTCATTTTGGAAAGACTCTAGCAACTTCTCAGATGTTTTTGATGAAGATCATTTTATCAAGGCTCTAGCTAATGATGTTAAAATTGTAAAAAAGCTTCCCAAGGAACTTGCTTCTGGTCCCAGAGCACTAAAGCTTTTCAGAAGCTGGGCTAGTATGGATTATTACCAAGATGAGATAGCTAGTCTCTGGGAAGTGTACGAGGTTATTCGAGCCTCCAAAGCTGATGCTCGCTTAGCAAATAACAACCTACCTCTAGATATACAGAAACTTCGCTGTCGTGCTTGTTATGAAGCTCTGCGTTTTACACCTCAAATTGAAGCAATGGGAAAATTGTTAGTTGATCGGATGAGGTCGTTTGGTCCTTTTATTGCCCTTCATTTACGATTTGAGAAGGACATGCTTGCCTTTAGTGGATGCACGCAGGATTTAACTCCATCTGAAGCTGATGAACTAAGGATCATCCGGGAGAACACAAAAACCTGGAGAAATAAGGAAATTAATTCCATAGAACAGAGATCCGGAGGGTATTGCCCATTAACGCCAAGGGAGACTGGAATTTTTCTCAGTGCTCTTGGGTACCCACCAAACACACCTATCTACATTGCTGCTGGAAAGATATATGGTGATTCTCGTATGGCTGATCTACTATCCCGTTATCCCATACTGAAGAGCAAGGAAGCATTGGCATCCGTTGAGGAGCTTGAACCTTTTACCAATCATGCGTCTCAAATGGCTGCGCTTGACTACATTGTTTCAGTTGAAAGTGCTGTATTTATACCTACATATCCAGGACATATGGCGGCGGCAGTTGAAGGTCACCGTCGCTTTCTTGGACACAGGAAAACAATAAACCCTGACAGAAAAGCCCTTGTTTCTCTGTTTGACAAACTTGAGCAGGGAACACTTGAGGAAGGCAAAACCTTATCAAATAGAGTTGTTCAAATGCACAGGAAACGGCAAGGCTCCCCACGGAAAAGAAGAGGCCCTGCTCCAGGACTTAGGGGGAAGGATAAGCTTCGTTCGGAAGAACCCTTTTATGAGAACCCCTTACCAGATTGTTTGTGTAGAAAAGAATTGCCAATGCAAAGAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

489

Amino Acids

55.33

Weight (kDa)

9.24

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 85 - 399 7.4e-100 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021880)

Species Orthologous Gene IDs
malus_domestica MD08G1188000.v1.1 MD15G1374000.v1.1
pyrus_communis pycom08g16140
rosa_chinensis RchiOBHm_Chr7g0242491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 945
AccB1I GGYRCC 1 cut(s) 945
AccIII TCCGGA 1 cut(s) 887
AciI CCGC 3 cut(s) 325, 1169, 1172
AclWI GGATC 2 cut(s) 833, 879
AcsI RAATTY 1 cut(s) 924
AcuI CTGAAG 2 cut(s) 828, 1055
AfaI GTAC 3 cut(s) 268, 566, 947
AfiI CCNNNNNNNGG 1 cut(s) 1361
AgsI TTSAA 6 cut(s) 260, 686, 1073, 1130, 1181, 1310
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 3 cut(s) 848, 1156, 1360
Alw21I GWGCWC 3 cut(s) 496, 940, 1462
Alw26I GTCTC 4 cut(s) 284, 557, 911, 1098
AlwI GGATC 2 cut(s) 833, 879
AlwNI CAGNNNCTG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 887
AoxI GGCC 1 cut(s) 1351
ApeKI GCWGC 2 cut(s) 974, 1103
ApoI RAATTY 1 cut(s) 924
AseI ATTAAT 1 cut(s) 867
Asp718I GGTACC 1 cut(s) 945
AspLEI GCGC 3 cut(s) 56, 73, 1108
AspS9I GGNCC 3 cut(s) 485, 728, 1352
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 2 cut(s) 1004, 1178
AvaII GGWCC 2 cut(s) 485, 728
BanI GGYRCC 1 cut(s) 945
BanII GRGCYC 1 cut(s) 1462
BarI GAAGNNNNNNTAC 2 cut(s) 447, 479
Bbv12I GWGCWC 3 cut(s) 496, 940, 1462
BbvI GCAGC 2 cut(s) 961, 1090
BccI CCATC 1 cut(s) 811
BceAI ACGGC 2 cut(s) 41, 1340
BcgI CGANNNNNNTGC 1 cut(s) 1442
BciT130I CCWGG 3 cut(s) 850, 1158, 1362
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 4 cut(s) 284, 557, 911, 1098
BfaI CTAG 6 cut(s) 74, 387, 434, 519, 549, 623
BfmI CTRYAG 2 cut(s) 237, 318
BisI GCNGC 4 cut(s) 975, 1104, 1170, 1173
BlpI GCTNAGC 1 cut(s) 601
BlsI GCNGC 4 cut(s) 976, 1105, 1171, 1174
Bme1390I CCNGG 4 cut(s) 833, 850, 1158, 1362
Bme18I GGWCC 2 cut(s) 485, 728
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 485, 728, 1352
BmiI GGNNCC 3 cut(s) 487, 947, 1333
BmrFI CCNGG 4 cut(s) 833, 850, 1158, 1362
BmsI GCATC 5 cut(s) 279, 336, 583, 773, 1064
BpmI CTGGAG 3 cut(s) 37, 871, 1344
Bpu1102I GCTNAGC 1 cut(s) 601
BpuEI CTTGAG 2 cut(s) 1280, 1295
BpuMI CCSGG 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 468, 911, 1337
BsaWI WCCGGW 1 cut(s) 887
Bsc4I CCNNNNNNNGG 1 cut(s) 1361
Bse1I ACTGG 1 cut(s) 925
Bse3DI GCAATG 2 cut(s) 696, 969
Bse8I GATNNNNATC 1 cut(s) 414
BseAI TCCGGA 1 cut(s) 887
BseBI CCWGG 3 cut(s) 850, 1158, 1362
BseDI CCNNGG 3 cut(s) 468, 911, 1337
BseGI GGATG 6 cut(s) 145, 166, 720, 788, 828, 1055
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 1361
BseMI GCAATG 2 cut(s) 696, 969
BseMII CTCAG 2 cut(s) 411, 946
BseNI ACTGG 1 cut(s) 925
BseRI GAGGAG 1 cut(s) 1079
BseXI GCAGC 2 cut(s) 961, 1090
BseYI CCCAGC 1 cut(s) 513
BsgI GTGCAG 2 cut(s) 221, 315
BshFI GGCC 1 cut(s) 1353
BshNI GGYRCC 1 cut(s) 945
BsiHKAI GWGCWC 3 cut(s) 496, 940, 1462
BsiSI CCGG 2 cut(s) 832, 888
BslFI GGGAC 2 cut(s) 86, 471
BslI CCNNNNNNNGG 1 cut(s) 1361
BsmAI GTCTC 4 cut(s) 284, 557, 911, 1098
BsmBI CGTCTC 1 cut(s) 1098
BsmFI GGGAC 2 cut(s) 86, 471
BsnI GGCC 1 cut(s) 1353
Bso31I GGTCTC 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 496, 940, 1462
Bsp13I TCCGGA 1 cut(s) 887
Bsp143I GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
Bsp1720I GCTNAGC 1 cut(s) 601
BspACI CCGC 3 cut(s) 325, 1169, 1172
BspANI GGCC 1 cut(s) 1353
BspCNI CTCAG 2 cut(s) 410, 945
BspEI TCCGGA 1 cut(s) 887
BspLI GGNNCC 3 cut(s) 487, 947, 1333
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 2 cut(s) 833, 879
BspQI GCTCTTC 2 cut(s) 5, 1031
BspT107I GGYRCC 1 cut(s) 945
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 2 cut(s) 696, 969
BsrI ACTGG 1 cut(s) 925
BssECI CCNNGG 3 cut(s) 468, 911, 1337
BssMI GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
BssT1I CCWWGG 2 cut(s) 468, 911
Bst2UI CCWGG 3 cut(s) 850, 1158, 1362
Bst4CI ACNGT 3 cut(s) 34, 369, 1190
Bst6I CTCTTC 4 cut(s) 5, 89, 1031, 1342
BstC8I GCNNGC 6 cut(s) 294, 325, 598, 771, 788, 1462
BstDEI CTNAG 5 cut(s) 397, 601, 821, 932, 1367
BstDSI CCRYGG 1 cut(s) 1337
BstEII GGTNACC 1 cut(s) 1184
BstENI CCTNNNNNAGG 1 cut(s) 1359
BstF5I GGATG 6 cut(s) 145, 166, 720, 788, 828, 1055
BstHHI GCGC 3 cut(s) 56, 73, 1108
BstKTI GATC 6 cut(s) 364, 418, 712, 828, 887, 1012
BstMAI GTCTC 4 cut(s) 284, 557, 911, 1098
BstMBI GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
BstMWI GCNNNNNNNGC 4 cut(s) 60, 77, 587, 602
BstNI CCWGG 3 cut(s) 850, 1158, 1362
BstNSI RCATGY 1 cut(s) 769
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 4 cut(s) 831, 848, 1156, 1360
BstSFI CTRYAG 2 cut(s) 237, 318
BstV1I GCAGC 2 cut(s) 961, 1090
BstX2I RGATCY 1 cut(s) 884
BstYI RGATCY 1 cut(s) 884
BsuRI GGCC 1 cut(s) 1353
BtgI CCRYGG 1 cut(s) 1337
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 6 cut(s) 145, 166, 720, 788, 828, 1055
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 3 cut(s) 39, 209, 940
Cac8I GCNNGC 6 cut(s) 294, 325, 598, 771, 788, 1462
CaiI CAGNNNCTG 1 cut(s) 513
CfoI GCGC 3 cut(s) 56, 73, 1108
Cfr13I GGNCC 3 cut(s) 485, 728, 1352
CseI GACGC 1 cut(s) 1080
Csp6I GTAC 3 cut(s) 267, 565, 946
CviAII CATG 3 cut(s) 310, 766, 1088
CviQI GTAC 3 cut(s) 267, 565, 946
DdeI CTNAG 5 cut(s) 397, 601, 821, 932, 1367
DpnI GATC 6 cut(s) 363, 417, 711, 827, 886, 1011
DpnII GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
Eam1104I CTCTTC 4 cut(s) 5, 89, 1031, 1342
EarI CTCTTC 4 cut(s) 5, 89, 1031, 1342
Ecl136II GAGCTC 1 cut(s) 1460
Eco130I CCWWGG 2 cut(s) 468, 911
Eco24I GRGCYC 1 cut(s) 1462
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 2 cut(s) 485, 728
Eco53kI GAGCTC 1 cut(s) 1460
Eco57I CTGAAG 2 cut(s) 828, 1055
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 1 cut(s) 1460
EcoNI CCTNNNNNAGG 1 cut(s) 1359
EcoO109I RGGNCCY 1 cut(s) 1352
EcoO65I GGTNACC 1 cut(s) 1184
EcoRII CCWGG 3 cut(s) 848, 1156, 1360
EcoT14I CCWWGG 2 cut(s) 468, 911
EcoT38I GRGCYC 1 cut(s) 1462
ErhI CCWWGG 2 cut(s) 468, 911
Esp3I CGTCTC 1 cut(s) 1098
FaeI CATG 3 cut(s) 313, 769, 1091
FaqI GGGAC 2 cut(s) 86, 471
FatI CATG 3 cut(s) 309, 765, 1087
FauI CCCGC 1 cut(s) 332
FauNDI CATATG 1 cut(s) 1164
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 4 cut(s) 975, 1104, 1170, 1173
FokI GGATG 6 cut(s) 152, 173, 727, 795, 815, 1042
FriOI GRGCYC 1 cut(s) 1462
Fsp4HI GCNGC 4 cut(s) 975, 1104, 1170, 1173
FspBI CTAG 6 cut(s) 74, 387, 434, 519, 549, 623
GlaI GCGC 3 cut(s) 55, 72, 1107
GluI GCNGC 4 cut(s) 975, 1104, 1170, 1173
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 3 cut(s) 37, 871, 1344
HaeIII GGCC 1 cut(s) 1353
HapII CCGG 2 cut(s) 832, 888
HgaI GACGC 1 cut(s) 1080
HhaI GCGC 3 cut(s) 56, 73, 1108
Hin1II CATG 3 cut(s) 313, 769, 1091
Hin6I GCGC 3 cut(s) 54, 71, 1106
HinP1I GCGC 3 cut(s) 54, 71, 1106
HindIII AAGCTT 4 cut(s) 125, 461, 500, 1382
HinfI GANTC 4 cut(s) 178, 242, 383, 995
HpaII CCGG 2 cut(s) 832, 888
HphI GGTGA 2 cut(s) 1004, 1178
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 8 cut(s) 115, 153, 222, 400, 509, 714, 808, 1393
Hpy188III TCNNGA 4 cut(s) 191, 353, 623, 888
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 1194
HpyAV CCTTC 6 cut(s) 34, 752, 754, 1175, 1274, 1369
HpyCH4III ACNGT 3 cut(s) 34, 369, 1190
HpyCH4IV ACGT 2 cut(s) 50, 134
HpyCH4V TGCA 7 cut(s) 4, 202, 239, 296, 786, 1315, 1453
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 77, 587, 602
HpyF3I CTNAG 5 cut(s) 397, 601, 821, 932, 1367
HpySE526I ACGT 2 cut(s) 50, 134
Hsp92II CATG 3 cut(s) 313, 769, 1091
HspAI GCGC 3 cut(s) 54, 71, 1106
Kpn2I TCCGGA 1 cut(s) 887
KpnI GGTACC 1 cut(s) 949
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
LguI GCTCTTC 2 cut(s) 5, 1031
LmnI GCTCC 3 cut(s) 1066, 1337, 1363
Lsp1109I GCAGC 2 cut(s) 961, 1090
LweI GCATC 5 cut(s) 279, 336, 583, 773, 1064
MaeI CTAG 6 cut(s) 74, 387, 434, 519, 549, 623
MaeII ACGT 2 cut(s) 50, 134
MaeIII GTNAC 3 cut(s) 243, 305, 1184
MalI GATC 6 cut(s) 363, 417, 711, 827, 886, 1011
MboI GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
MboII GAAGA 7 cut(s) 22, 76, 187, 425, 1048, 1359, 1406
MflI RGATCY 1 cut(s) 884
MhlI GDGCHC 3 cut(s) 496, 940, 1462
MluCI AATT 8 cut(s) 66, 450, 681, 698, 864, 868, 924, 1442
MlyI GAGTC 2 cut(s) 251, 377
MmeI TCCRAC 3 cut(s) 117, 131, 138
MroI TCCGGA 1 cut(s) 887
MseI TTAA 5 cut(s) 65, 447, 797, 867, 905
MspI CCGG 2 cut(s) 832, 888
MspR9I CCNGG 4 cut(s) 833, 850, 1158, 1362
MvaI CCWGG 3 cut(s) 850, 1158, 1362
MwoI GCNNNNNNNGC 4 cut(s) 60, 77, 587, 602
NciI CCSGG 1 cut(s) 833
NdeI CATATG 1 cut(s) 1164
NdeII GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
NlaIII CATG 3 cut(s) 313, 769, 1091
NlaIV GGNNCC 3 cut(s) 487, 947, 1333
NmuCI GTSAC 3 cut(s) 243, 305, 1184
NspI RCATGY 1 cut(s) 769
PciSI GCTCTTC 2 cut(s) 5, 1031
PfeI GAWTC 2 cut(s) 178, 995
PflFI GACNNNGTC 1 cut(s) 119
PfoI TCCNGGA 3 cut(s) 831, 1156, 1360
PkrI GCNGC 4 cut(s) 976, 1105, 1171, 1174
PleI GAGTC 2 cut(s) 250, 377
PpsI GAGTC 2 cut(s) 250, 377
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 1462
Psp6I CCWGG 3 cut(s) 848, 1156, 1360
PspEI GGTNACC 1 cut(s) 1184
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 3 cut(s) 848, 1156, 1360
PspN4I GGNNCC 3 cut(s) 487, 947, 1333
PspPI GGNCC 3 cut(s) 485, 728, 1352
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 513
PsuI RGATCY 1 cut(s) 884
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 3 cut(s) 268, 566, 947
RsaNI GTAC 3 cut(s) 267, 565, 946
SacI GAGCTC 1 cut(s) 1462
SapI GCTCTTC 2 cut(s) 5, 1031
SaqAI TTAA 5 cut(s) 65, 447, 797, 867, 905
SatI GCNGC 4 cut(s) 975, 1104, 1170, 1173
Sau3AI GATC 6 cut(s) 361, 415, 709, 825, 884, 1009
Sau96I GGNCC 3 cut(s) 485, 728, 1352
SchI GAGTC 2 cut(s) 251, 377
ScrFI CCNGG 4 cut(s) 833, 850, 1158, 1362
SduI GDGCHC 3 cut(s) 496, 940, 1462
SfaNI GCATC 5 cut(s) 279, 336, 583, 773, 1064
SfcI CTRYAG 2 cut(s) 237, 318
SinI GGWCC 2 cut(s) 485, 728
SmlI CTYRAG 2 cut(s) 1259, 1274
SmoI CTYRAG 2 cut(s) 1259, 1274
Sse9I AATT 8 cut(s) 66, 450, 681, 698, 864, 868, 924, 1442
SsiI CCGC 3 cut(s) 325, 1169, 1172
SspMI CTAG 6 cut(s) 74, 387, 434, 519, 549, 623
SstI GAGCTC 1 cut(s) 1462
StyD4I CCNGG 4 cut(s) 831, 848, 1156, 1360
StyI CCWWGG 2 cut(s) 468, 911
TaaI ACNGT 3 cut(s) 34, 369, 1190
TaiI ACGT 2 cut(s) 53, 137
TaqI TCGA 1 cut(s) 577
TasI AATT 8 cut(s) 66, 450, 681, 698, 864, 868, 924, 1442
TauI GCSGC 2 cut(s) 1172, 1175
TfiI GAWTC 2 cut(s) 178, 995
Tru1I TTAA 5 cut(s) 65, 447, 797, 867, 905
Tru9I TTAA 5 cut(s) 65, 447, 797, 867, 905
TscAI CASTG 3 cut(s) 39, 209, 940
TseFI GTSAC 3 cut(s) 243, 305, 1184
TseI GCWGC 2 cut(s) 974, 1103
Tsp45I GTSAC 3 cut(s) 243, 305, 1184
TspDTI ATGAA 4 cut(s) 426, 672, 734, 831
TspGWI ACGGA 2 cut(s) 1048, 1354
TspRI CASTG 3 cut(s) 39, 209, 940
Tth111I GACNNNGTC 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 485, 728
VspI ATTAAT 1 cut(s) 867
XagI CCTNNNNNAGG 1 cut(s) 1359
XapI RAATTY 1 cut(s) 924
XbaI TCTAGA 1 cut(s) 622
XceI RCATGY 1 cut(s) 769
XspI CTAG 6 cut(s) 74, 387, 434, 519, 549, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.