pycom08g18610

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029355
Physical Location & Seq
Forward (+)
18738068 .. 18738528
461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18610.1

Sequence Viewer

Length: 411 bp
ATGGAGGGCCCTGGAATTGCAGCCATAATCTTGTCCTGGGTGATCACTTTCTACTCATTCTGGCAACTGATAAGACTGCATGAAATAGTTCCCGGACAGCGTTTTGATCGATACCCAGAACTAGGGCAGCATTGTTTTGGGCCAAAACTAGGGTACTGGATTATTATGCCGCAGCAGGTGACCGTGCAAGTGGCTTCATCCATTGTGTATACAGTCACTGGTGGCAAGTCATTGAAAAAATTCTTCGAACTTGTTATTCCAAGCATAGGTGATATTAAACAAACATATTTCATCCTCTTCTTCACAATTTTGCAATTAGGGATCTCCCAATCTCCCAATTTCAATTCTTTGAAGGGAATTTCTCTTCTCGCGGCATTCATGTCTTTCGGGTATATATTCCTTCCTTGTTAA

Protein Analysis

137

Amino Acids

15.39

Weight (kDa)

8.98

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 4 - 132 8.2e-27 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029355)

Species Orthologous Gene IDs
pyrus_communis pycom08g18610
rosa_multiflora Rmu_sc0001192.1_g000018

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 209
AccII CGCG 1 cut(s) 371
AciI CCGC 2 cut(s) 170, 371
AclWI GGATC 1 cut(s) 329
AcsI RAATTY 2 cut(s) 239, 357
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 122, 149, 266
AgsI TTSAA 3 cut(s) 235, 343, 352
AjnI CCWGG 2 cut(s) 10, 35
AlwI GGATC 1 cut(s) 329
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 2 cut(s) 7, 140
ApaI GGGCCC 1 cut(s) 11
ApeKI GCWGC 3 cut(s) 20, 127, 172
ApoI RAATTY 2 cut(s) 239, 357
Asp700I GAANNNNTTC 2 cut(s) 87, 239
AspS9I GGNCC 3 cut(s) 7, 8, 140
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 3 cut(s) 52, 190, 281
AsuII TTCGAA 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 32, 139, 184
BciT130I CCWGG 2 cut(s) 12, 37
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 1 cut(s) 93
BfaI CTAG 2 cut(s) 122, 149
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 5 cut(s) 21, 128, 170, 173, 372
BlsI GCNGC 5 cut(s) 22, 129, 171, 174, 373
Bme1390I CCNGG 3 cut(s) 12, 37, 93
BmgT120I GGNCC 3 cut(s) 7, 8, 140
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 3 cut(s) 12, 37, 93
Bpu14I TTCGAA 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 93
Bsa29I ATCGAT 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 10, 36
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 149, 266
Bse1I ACTGG 2 cut(s) 161, 223
BseBI CCWGG 2 cut(s) 12, 37
BseCI ATCGAT 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 10, 36
BseGI GGATG 2 cut(s) 197, 291
BseLI CCNNNNNNNGG 3 cut(s) 122, 149, 266
BseNI ACTGG 2 cut(s) 161, 223
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 3 cut(s) 32, 139, 184
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 2 cut(s) 9, 142
BshVI ATCGAT 1 cut(s) 109
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 122, 149, 266
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 2 cut(s) 9, 142
Bsp119I TTCGAA 1 cut(s) 246
Bsp120I GGGCCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 42, 106, 321
BspACI CCGC 2 cut(s) 170, 371
BspANI GGCC 2 cut(s) 9, 142
BspDI ATCGAT 1 cut(s) 109
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 9
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 246
BsrI ACTGG 2 cut(s) 161, 223
BssECI CCNNGG 2 cut(s) 10, 36
BssMI GATC 3 cut(s) 42, 106, 321
BssNAI GTATAC 1 cut(s) 210
Bst1107I GTATAC 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 12, 37
Bst4CI ACNGT 2 cut(s) 184, 214
Bst6I CTCTTC 2 cut(s) 302, 369
BstBI TTCGAA 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 2 cut(s) 197, 291
BstFNI CGCG 1 cut(s) 371
BstKTI GATC 3 cut(s) 45, 109, 324
BstMBI GATC 3 cut(s) 42, 106, 321
BstNI CCWGG 2 cut(s) 12, 37
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 3 cut(s) 10, 35, 91
BstSLI GKGCMC 1 cut(s) 11
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 32, 139, 184
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BstZ17I GTATAC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 109
BsuRI GGCC 2 cut(s) 9, 142
BsuTUI ATCGAT 1 cut(s) 109
BtsCI GGATG 2 cut(s) 197, 291
BtsIMutI CAGTG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 166
CaiI CAGNNNCTG 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 7, 8, 140
ClaI ATCGAT 1 cut(s) 109
Csp6I GTAC 1 cut(s) 154
CviAII CATG 2 cut(s) 80, 379
CviJI RGCY 4 cut(s) 9, 23, 142, 194
CviKI_1 RGCY 4 cut(s) 9, 23, 142, 194
CviQI GTAC 1 cut(s) 154
DpnI GATC 3 cut(s) 44, 108, 323
DpnII GATC 3 cut(s) 42, 106, 321
Eam1104I CTCTTC 2 cut(s) 302, 369
EarI CTCTTC 2 cut(s) 302, 369
Eco24I GRGCYC 1 cut(s) 11
Eco91I GGTNACC 1 cut(s) 178
EcoO109I RGGNCCY 2 cut(s) 7, 8
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 10, 35
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 2 cut(s) 83, 382
FaiI YATR 9 cut(s) 26, 81, 167, 210, 266, 286, 380, 393, 395
FatI CATG 2 cut(s) 79, 378
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 5 cut(s) 21, 128, 170, 173, 372
FokI GGATG 2 cut(s) 184, 278
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 5 cut(s) 21, 128, 170, 173, 372
FspBI CTAG 2 cut(s) 122, 149
GluI GCNGC 5 cut(s) 21, 128, 170, 173, 372
HaeIII GGCC 2 cut(s) 9, 142
HapII CCGG 1 cut(s) 93
Hin1II CATG 2 cut(s) 83, 382
HpaII CCGG 1 cut(s) 93
HphI GGTGA 3 cut(s) 52, 190, 281
Hpy166II GTNNAC 1 cut(s) 210
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 2 cut(s) 346, 410
HpyCH4III ACNGT 2 cut(s) 184, 214
HpyCH4V TGCA 4 cut(s) 20, 79, 187, 313
Hsp92II CATG 2 cut(s) 83, 382
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 3 cut(s) 42, 106, 321
LpnPI CCDG 9 cut(s) 22, 24, 46, 49, 106, 129, 142, 161, 204
Lsp1109I GCAGC 3 cut(s) 32, 139, 184
MaeI CTAG 2 cut(s) 122, 149
MaeIII GTNAC 2 cut(s) 178, 214
MalI GATC 3 cut(s) 44, 108, 323
MboI GATC 3 cut(s) 42, 106, 321
MboII GAAGA 4 cut(s) 235, 289, 292, 356
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 7 cut(s) 15, 239, 306, 314, 337, 343, 357
MnlI CCTC 1 cut(s) 305
MroXI GAANNNNTTC 2 cut(s) 87, 239
MseI TTAA 2 cut(s) 276, 409
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 3 cut(s) 12, 37, 93
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 2 cut(s) 12, 37
MvnI CGCG 1 cut(s) 371
NciI CCSGG 1 cut(s) 93
NdeII GATC 3 cut(s) 42, 106, 321
NlaIII CATG 2 cut(s) 83, 382
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 178, 214
NspV TTCGAA 1 cut(s) 246
PaqCI CACCTGC 1 cut(s) 166
PctI GAATGC 1 cut(s) 374
PdmI GAANNNNTTC 2 cut(s) 87, 239
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 5 cut(s) 22, 129, 171, 174, 373
Psp6I CCWGG 2 cut(s) 10, 35
PspEI GGTNACC 1 cut(s) 178
PspGI CCWGG 2 cut(s) 10, 35
PspN4I GGNNCC 1 cut(s) 9
PspOMI GGGCCC 1 cut(s) 7
PspPI GGNCC 3 cut(s) 7, 8, 140
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 1 cut(s) 321
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SaqAI TTAA 2 cut(s) 276, 409
SatI GCNGC 5 cut(s) 21, 128, 170, 173, 372
Sau3AI GATC 3 cut(s) 42, 106, 321
Sau96I GGNCC 3 cut(s) 7, 8, 140
ScrFI CCNGG 3 cut(s) 12, 37, 93
SduI GDGCHC 1 cut(s) 11
SetI ASST 2 cut(s) 180, 271
SfuI TTCGAA 1 cut(s) 246
Sse9I AATT 7 cut(s) 15, 239, 306, 314, 337, 343, 357
SsiI CCGC 2 cut(s) 170, 371
SspMI CTAG 2 cut(s) 122, 149
StyD4I CCNGG 3 cut(s) 10, 35, 91
TaaI ACNGT 2 cut(s) 184, 214
TaqI TCGA 2 cut(s) 109, 246
TasI AATT 7 cut(s) 15, 239, 306, 314, 337, 343, 357
TauI GCSGC 2 cut(s) 172, 374
Tru1I TTAA 2 cut(s) 276, 409
Tru9I TTAA 2 cut(s) 276, 409
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 2 cut(s) 178, 214
TseI GCWGC 3 cut(s) 20, 127, 172
Tsp45I GTSAC 2 cut(s) 178, 214
TspDTI ATGAA 4 cut(s) 96, 186, 280, 367
TspRI CASTG 1 cut(s) 223
XapI RAATTY 2 cut(s) 239, 357
XmiI GTMKAC 1 cut(s) 209
XmnI GAANNNNTTC 2 cut(s) 87, 239
XspI CTAG 2 cut(s) 122, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.