pycom08g19540

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029357
Physical Location & Seq
Forward (+)
19592527 .. 19596215
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19540.1

Sequence Viewer

Length: 726 bp
ATGTCATTCCGTAGCATAGTTCGTGATGTTAGGGATGGCTTTGGGAGCTTATCTAGACGGGGTTTTGAAGTCCGGCTGACGAGCCATCATAGAGGAAAATCTCATGGCTCGTTAAATGATTTACATGACCAGCCTCTCATTGTTCAGAACTGTCGTTGGGCTAGCCTCCCCGCAGAACTATTATATGATGTAATTAGGAGGCTGGAGGAGAGTGAGAGCACATGGCCTTCTCGAAAGCACGTTGTTGCATGTGCCTCAGTTTGCCGTTCTTGGAGGATTATGTGCAAAGAAATCGTTAAGAAGCCTGAGATCTGCGGGAAGCTTACTTTTCCTGTGTCGCTGAAGCAGCCAGGTTCACGGGATGGACTCGTTCAATGCTTCATTAAAAGGGATAAATCCAATTTAACATATCATCTATTTCTGTGTCTTAGCCCTGCTTTGCTAGTTGAGAATGGGAAATTCCTTCTTTCTGCAAAGAGGACTCGAAGAACTACTTGTACAGAATATGTTATCTCTATGGATGCTGACAACATCTCTAACCGTTGGGATCAAGCAGCAGTTACATTGGAAAGTCAAACTTTCTTGGCACAAAATTCATTGTATATGATACGCAGCCTGCATACACTTCTGAACATGTTCCCCCACCAGGGCGGTCAAGTCGTAGATTTAATTCCAAAAAAGTCTCGCCAAAAGTCCCGACTGGCAGCTATAACATTGCTCAGATAA

Protein Analysis

242

Amino Acids

27.54

Weight (kDa)

9.88

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 114 - 201 5.6e-20 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029357)

Species Orthologous Gene IDs
pyrus_communis pycom08g19540 pycom15g37080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 171, 315, 651
AclWI GGATC 1 cut(s) 555
AcsI RAATTY 2 cut(s) 458, 592
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 499
AfiI CCNNNNNNNGG 2 cut(s) 646, 647
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 2 cut(s) 68, 374
AjnI CCWGG 2 cut(s) 349, 645
AluBI AGCT 3 cut(s) 48, 322, 707
AluI AGCT 3 cut(s) 48, 322, 707
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 687
AlwI GGATC 1 cut(s) 555
AoxI GGCC 1 cut(s) 224
ApeKI GCWGC 4 cut(s) 346, 554, 612, 704
ApoI RAATTY 2 cut(s) 458, 592
Asp700I GAANNNNTTC 1 cut(s) 635
AsuNHI GCTAGC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 4 cut(s) 358, 566, 624, 716
BccI CCATC 3 cut(s) 29, 93, 356
BceAI ACGGC 1 cut(s) 249
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 2 cut(s) 351, 647
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 3 cut(s) 54, 162, 443
BglII AGATCT 1 cut(s) 309
BisI GCNGC 4 cut(s) 347, 555, 613, 705
BlsI GCNGC 4 cut(s) 348, 556, 614, 706
Bme1390I CCNGG 2 cut(s) 351, 647
BmrFI CCNGG 2 cut(s) 351, 647
BmsI GCATC 1 cut(s) 511
BmtI GCTAGC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 646
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 647
Bse1I ACTGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 713
BseBI CCWGG 2 cut(s) 351, 647
BseDI CCNNGG 1 cut(s) 646
BseGI GGATG 3 cut(s) 40, 367, 526
BseLI CCNNNNNNNGG 2 cut(s) 646, 647
BseMI GCAATG 1 cut(s) 713
BseMII CTCAG 2 cut(s) 270, 297
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 4 cut(s) 358, 566, 624, 716
BshFI GGCC 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 1 cut(s) 73
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 2 cut(s) 646, 647
BsmAI GTCTC 1 cut(s) 687
BsmFI GGGAC 1 cut(s) 679
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 497
Bsp143I GATC 2 cut(s) 309, 547
BspACI CCGC 3 cut(s) 171, 315, 651
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 2 cut(s) 269, 298
BspOI GCTAGC 1 cut(s) 165
BspPI GGATC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 713
BsrGI TGTACA 1 cut(s) 497
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 1 cut(s) 646
BssMI GATC 2 cut(s) 309, 547
Bst2UI CCWGG 2 cut(s) 351, 647
Bst4CI ACNGT 2 cut(s) 152, 542
BstAUI TGTACA 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 163, 617
BstDEI CTNAG 4 cut(s) 256, 306, 428, 719
BstF5I GGATG 3 cut(s) 40, 367, 526
BstKTI GATC 2 cut(s) 312, 550
BstMAI GTCTC 1 cut(s) 687
BstMBI GATC 2 cut(s) 309, 547
BstMWI GCNNNNNNNGC 2 cut(s) 45, 346
BstNI CCWGG 2 cut(s) 351, 647
BstNSI RCATGY 2 cut(s) 252, 637
BstSCI CCNGG 2 cut(s) 349, 645
BstV1I GCAGC 4 cut(s) 358, 566, 624, 716
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
BsuRI GGCC 1 cut(s) 226
BtsCI GGATG 3 cut(s) 40, 367, 526
Cac8I GCNNGC 2 cut(s) 163, 617
Csp6I GTAC 1 cut(s) 498
CviAII CATG 5 cut(s) 104, 125, 222, 249, 634
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 4 cut(s) 256, 306, 428, 719
DpnI GATC 2 cut(s) 311, 549
DpnII GATC 2 cut(s) 309, 547
Eco57I CTGAAG 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 349, 645
FaeI CATG 5 cut(s) 107, 128, 225, 252, 637
FalI AAGNNNNNCTT 4 cut(s) 478, 510, 562, 594
FaqI GGGAC 1 cut(s) 679
FatI CATG 5 cut(s) 103, 124, 221, 248, 633
FauI CCCGC 2 cut(s) 178, 308
Fnu4HI GCNGC 4 cut(s) 347, 555, 613, 705
FokI GGATG 3 cut(s) 47, 374, 533
Fsp4HI GCNGC 4 cut(s) 347, 555, 613, 705
FspBI CTAG 3 cut(s) 54, 162, 443
GluI GCNGC 4 cut(s) 347, 555, 613, 705
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 73
Hin1II CATG 5 cut(s) 107, 128, 225, 252, 637
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 2 cut(s) 366, 481
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 3 cut(s) 147, 630, 722
Hpy188III TCNNGA 4 cut(s) 23, 54, 231, 696
Hpy8I GTNNAC 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 237, 473
HpyCH4III ACNGT 2 cut(s) 152, 542
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 4 cut(s) 248, 285, 473, 619
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 346
HpyF3I CTNAG 4 cut(s) 256, 306, 428, 719
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 5 cut(s) 107, 128, 225, 252, 637
Kzo9I GATC 2 cut(s) 309, 547
LmnI GCTCC 1 cut(s) 45
Lsp1109I GCAGC 4 cut(s) 358, 566, 624, 716
LweI GCATC 1 cut(s) 511
MaeI CTAG 3 cut(s) 54, 162, 443
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 559
MalI GATC 2 cut(s) 311, 549
MboI GATC 2 cut(s) 309, 547
MboII GAAGA 1 cut(s) 498
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 5 cut(s) 192, 400, 458, 592, 669
MlyI GAGTC 2 cut(s) 360, 475
MnlI CCTC 8 cut(s) 86, 144, 176, 192, 199, 265, 267, 471
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 5 cut(s) 113, 297, 384, 404, 668
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 2 cut(s) 351, 647
MvaI CCWGG 2 cut(s) 351, 647
MwoI GCNNNNNNNGC 2 cut(s) 45, 346
NdeII GATC 2 cut(s) 309, 547
NheI GCTAGC 1 cut(s) 161
NlaIII CATG 5 cut(s) 107, 128, 225, 252, 637
NspI RCATGY 2 cut(s) 252, 637
PciI ACATGT 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 635
PkrI GCNGC 4 cut(s) 348, 556, 614, 706
PleI GAGTC 2 cut(s) 360, 475
PpsI GAGTC 2 cut(s) 360, 475
PscI ACATGT 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 349, 645
PspGI CCWGG 2 cut(s) 349, 645
PsrI GAACNNNNNNTAC 2 cut(s) 481, 513
PsuI RGATCY 1 cut(s) 309
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
SaqAI TTAA 5 cut(s) 113, 297, 384, 404, 668
SatI GCNGC 4 cut(s) 347, 555, 613, 705
Sau3AI GATC 2 cut(s) 309, 547
SchI GAGTC 2 cut(s) 360, 475
ScrFI CCNGG 2 cut(s) 351, 647
SduI GDGCHC 1 cut(s) 221
SetI ASST 5 cut(s) 50, 243, 324, 355, 709
SfaNI GCATC 1 cut(s) 511
Sse9I AATT 5 cut(s) 192, 400, 458, 592, 669
SsiI CCGC 3 cut(s) 171, 315, 651
SspMI CTAG 3 cut(s) 54, 162, 443
StyD4I CCNGG 2 cut(s) 349, 645
TaaI ACNGT 2 cut(s) 152, 542
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 232, 484
TasI AATT 5 cut(s) 192, 400, 458, 592, 669
TatI WGTACW 1 cut(s) 497
Tru1I TTAA 5 cut(s) 113, 297, 384, 404, 668
Tru9I TTAA 5 cut(s) 113, 297, 384, 404, 668
TseI GCWGC 4 cut(s) 346, 554, 612, 704
TspDTI ATGAA 2 cut(s) 370, 585
XapI RAATTY 2 cut(s) 458, 592
XbaI TCTAGA 1 cut(s) 53
XceI RCATGY 2 cut(s) 252, 637
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 3 cut(s) 54, 162, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.