pycom08g20890
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0024392
Physical Location & Seq
Reverse (-)
20505846 .. 20508282
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGAGGAATGCAGGAAGTGATGAGTTTGCTGGTGATGGGGAAATACAGGAGCAGCAGAAGCAGAGGTGGTATTCATCATCTGCATCAAAGTTGGTGCTGCCTCAACTGTGTTTAAATCACGTGTCGTTTGTTTGCAAGTCGGTGAGCAAATCAGTGAGGTTCTACGAGGATGTCTTAGGTTTTGTGCTCATCCAACGCCCTTCTTCCTTCAAGTTTGAAGGAGCTTGGCTGTTCAACCATGGAGTGGGAATTCATCTGCTGGAGTCGGATACCATCAATGCCGCGGAAAAGAAATGCAGAAAGATAAACCCAAAGGACAACCACATCTCGTTCCAGTGCTCAGACATGGCCGTGGTGGTGCAGAAACTGGAGGAGATGAAGGTAGAGTACGTGACGGCGCAGGTAGAAGAAGGCGGAGTTACAGTTGATCAGCTCTTCTTCCATGACCCTGATGGCTACATGCTTGAAATTTGCAACTGTCATAATCTTCCAGTCCTTCCCATCTCCTCCTCATGCCCTCTCAACCTAATCACCAACTCCAACCCAAACCATGCATCGTCGTCTTCGTCATCATCGTCGTCCTTCTACGGGAAGCGAAGTTCAGAGAAACCGTGTTCTGGAGAAGCAGCAACGCTAATGATGGAGAGTTTGGTGATGGATGTGATGAACATTTCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

227

Amino Acids

25.06

Weight (kDa)

5.48

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 38 - 157 1.2e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 39 - 139 1.5e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 43 - 159 4.6e-07 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024392)

Species Orthologous Gene IDs
malus_domestica MD08G1241000.v1.1
prunus_persica Prupe.1G577800_v2.0.a1
pyrus_communis pycom08g20890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 244
AccII CGCG 1 cut(s) 284
AciI CCGC 3 cut(s) 282, 284, 414
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 2 cut(s) 249, 468
AcvI CACGTG 1 cut(s) 121
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 3 cut(s) 244, 588, 617
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 4 cut(s) 211, 218, 235, 467
AloI GAACNNNNNNTCC 2 cut(s) 583, 615
AluBI AGCT 2 cut(s) 224, 433
AluI AGCT 2 cut(s) 224, 433
Alw21I GWGCWC 2 cut(s) 189, 341
AlwNI CAGNNNCTG 1 cut(s) 367
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 3 cut(s) 52, 97, 626
ApoI RAATTY 2 cut(s) 249, 468
AspLEI GCGC 1 cut(s) 400
AsuHPI GGTGA 4 cut(s) 44, 154, 523, 664
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbrPI CACGTG 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 2 cut(s) 189, 341
BbvI GCAGC 3 cut(s) 64, 84, 638
BccI CCATC 6 cut(s) 29, 281, 446, 509, 634, 649
BceAI ACGGC 2 cut(s) 335, 411
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 4 cut(s) 53, 98, 282, 627
BlsI GCNGC 4 cut(s) 54, 99, 283, 628
BmsI GCATC 2 cut(s) 92, 563
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 3 cut(s) 281, 389, 639
BsaAI YACGTR 2 cut(s) 121, 391
BsaJI CCNNGG 3 cut(s) 238, 282, 351
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 588, 617
Bse1I ACTGG 3 cut(s) 334, 372, 491
BseDI CCNNGG 3 cut(s) 238, 282, 351
BseGI GGATG 3 cut(s) 175, 189, 664
BseLI CCNNNNNNNGG 3 cut(s) 244, 588, 617
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 3 cut(s) 334, 372, 491
BseRI GAGGAG 3 cut(s) 386, 496, 499
BseXI GCAGC 3 cut(s) 64, 84, 638
BsgI GTGCAG 1 cut(s) 380
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 1 cut(s) 350
BsiHKAI GWGCWC 2 cut(s) 189, 341
BslI CCNNNNNNNGG 3 cut(s) 244, 588, 617
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 189, 341
Bsp143I GATC 1 cut(s) 427
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 3 cut(s) 282, 284, 414
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 1 cut(s) 353
BspFNI CGCG 1 cut(s) 284
BspMI ACCTGC 1 cut(s) 391
BspQI GCTCTTC 1 cut(s) 440
BsrI ACTGG 3 cut(s) 334, 372, 491
BssECI CCNNGG 3 cut(s) 238, 282, 351
BssMI GATC 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 4 cut(s) 108, 424, 479, 612
Bst6I CTCTTC 1 cut(s) 440
BstBAI YACGTR 2 cut(s) 121, 391
BstDEI CTNAG 2 cut(s) 175, 340
BstDSI CCRYGG 3 cut(s) 238, 282, 351
BstF5I GGATG 3 cut(s) 175, 189, 664
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 1 cut(s) 430
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 463
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 3 cut(s) 64, 84, 638
BstV2I GAAGAC 1 cut(s) 555
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 3 cut(s) 238, 282, 351
BtsCI GGATG 3 cut(s) 175, 189, 664
BtsIMutI CAGTG 2 cut(s) 159, 341
BveI ACCTGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 1 cut(s) 400
Cfr42I CCGCGG 1 cut(s) 285
Csp6I GTAC 1 cut(s) 388
CviAII CATG 7 cut(s) 239, 346, 443, 460, 513, 551, 676
CviJI RGCY 5 cut(s) 224, 229, 350, 433, 456
CviKI_1 RGCY 5 cut(s) 224, 229, 350, 433, 456
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 2 cut(s) 175, 340
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
DraI TTTAAA 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
EciI GGCGGA 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 238
Eco72I CACGTG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 7 cut(s) 242, 349, 446, 463, 516, 554, 679
FaiI YATR 8 cut(s) 240, 347, 444, 461, 483, 514, 552, 677
FatI CATG 7 cut(s) 238, 345, 442, 459, 512, 550, 675
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 4 cut(s) 53, 98, 282, 627
FokI GGATG 3 cut(s) 176, 182, 671
Fsp4HI GCNGC 4 cut(s) 53, 98, 282, 627
GlaI GCGC 1 cut(s) 399
GluI GCNGC 4 cut(s) 53, 98, 282, 627
GsuI CTGGAG 3 cut(s) 281, 389, 639
HaeIII GGCC 1 cut(s) 350
HhaI GCGC 1 cut(s) 400
Hin1II CATG 7 cut(s) 242, 349, 446, 463, 516, 554, 679
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HinfI GANTC 1 cut(s) 263
HphI GGTGA 4 cut(s) 44, 154, 523, 664
Hpy188I TCNGA 3 cut(s) 268, 343, 604
Hpy188III TCNNGA 1 cut(s) 618
Hpy99I CGWCG 2 cut(s) 562, 580
HpyAV CCTTC 7 cut(s) 210, 212, 217, 373, 404, 506, 592
HpyCH4III ACNGT 4 cut(s) 108, 424, 479, 612
HpyCH4IV ACGT 2 cut(s) 120, 390
HpyCH4V TGCA 7 cut(s) 11, 83, 135, 297, 361, 474, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 2 cut(s) 175, 340
HpySE526I ACGT 2 cut(s) 120, 390
Hsp92II CATG 7 cut(s) 242, 349, 446, 463, 516, 554, 679
HspAI GCGC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 427
KspI CCGCGG 1 cut(s) 285
Kzo9I GATC 1 cut(s) 427
LguI GCTCTTC 1 cut(s) 440
LmnI GCTCC 2 cut(s) 49, 221
LpnPI CCDG 9 cut(s) 15, 32, 245, 347, 353, 386, 462, 504, 603
Lsp1109I GCAGC 3 cut(s) 64, 84, 638
LweI GCATC 2 cut(s) 92, 563
MaeII ACGT 2 cut(s) 120, 390
MaeIII GTNAC 2 cut(s) 391, 418
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 6 cut(s) 195, 419, 427, 430, 479, 555
MhlI GDGCHC 2 cut(s) 189, 341
MluCI AATT 2 cut(s) 249, 468
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 3 cut(s) 217, 246, 564
MnlI CCTC 8 cut(s) 57, 111, 150, 160, 364, 517, 520, 528
Mph1103I ATGCAT 1 cut(s) 556
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 2 cut(s) 350, 674
MspA1I CMGCKG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 13
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 58
NcoI CCATGG 1 cut(s) 238
NdeII GATC 1 cut(s) 427
NlaIII CATG 7 cut(s) 242, 349, 446, 463, 516, 554, 679
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 563, 572
PctI GAATGC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 4 cut(s) 54, 99, 283, 628
PleI GAGTC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 121
PmlI CACGTG 1 cut(s) 121
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 2 cut(s) 121, 391
PspCI CACGTG 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 367
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 2 cut(s) 350, 674
SacII CCGCGG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 440
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 4 cut(s) 53, 98, 282, 627
Sau3AI GATC 1 cut(s) 427
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 2 cut(s) 189, 341
SfaNI GCATC 2 cut(s) 92, 563
Sfr303I CCGCGG 1 cut(s) 285
SgrBI CCGCGG 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 350, 674
Sse9I AATT 2 cut(s) 249, 468
SsiI CCGC 3 cut(s) 282, 284, 414
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 4 cut(s) 108, 424, 479, 612
TaiI ACGT 2 cut(s) 123, 393
TasI AATT 2 cut(s) 249, 468
TauI GCSGC 1 cut(s) 284
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 2 cut(s) 159, 341
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 3 cut(s) 52, 97, 626
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 4 cut(s) 63, 242, 392, 680
TspRI CASTG 2 cut(s) 159, 341
Van91I CCANNNNNTGG 1 cut(s) 244
XapI RAATTY 2 cut(s) 249, 468
XceI RCATGY 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.