pycom09g03910

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0020425
Physical Location & Seq
Forward (+)
2914802 .. 2915731
930 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGGAGAACTTCCCAGTTATCAACTTGGAGAGCCTCAATGGTGAGGGAAGAAAAGCTACAATGGAAAAGATCAAAGATGCCTGTGAGAACTGGGGTTTCTTTGAGCTGGTGAGTCATGGGATTCCAACTGAGTTTCTGGACACAGTGGAGAGGTTGACAAAAGAGCACTACAAGCAGTGTTTGGAGCAAAGATTCAAGGAGCTGGTGGCCAGCAAAGGCCTTGAGGGTGTTCAGACAGAAGTCAAAGATATGGATTGGGAAAGCACTTTCCACTTGCGCCATCTTCCTCAATCGAACATCTCTGAAGTACCAGATCTCAAGGATGAGTACAGGAATGTGATGAAGGAGTTTGCATTGAAATTGGAAAAATTAGCAGAGCAGCTGCTGGACTTGTTGTGTGAGAATCTTGGACTGGAACAAGGGTACCTTAAGAAAGCATTTTATGGAACAAAGGGACCAACTTTTGGCACCAAGGTGAGCAACTACCCTCCATGTCCTAACCCAGACCTGATCAAGGGTTCACACACTCCGGGCCCACACCGATGCCGGCGGACTCATCTTGCTCTTCCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009506 GO:0009593 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009735 GO:0009815 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010817 GO:0012505 GO:0016020 GO:0016491 GO:0016638 GO:0016641 GO:0030054 GO:0030312 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043449 GO:0043450 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048518 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0055114 GO:0065007 GO:0065008 GO:0070542 GO:0070887 GO:0071229 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071396 GO:0071398 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0097366 GO:1900140 GO:1900673 GO:1900674 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

192

Amino Acids

21.83

Weight (kDa)

6.0

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 5 - 98 6.7e-16 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020425)

Species Orthologous Gene IDs
prunus_persica Prupe.3G209900_v2.0.a1
pyrus_communis pycom09g03910
rosa_multiflora Rmu_co8354249.1_g000001
rosa_samantha Rh5AG014500 Rh5BG017900 Rh5CG015600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 467
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 3 cut(s) 309, 329, 425
AfiI CCNNNNNNNGG 2 cut(s) 464, 514
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 2 cut(s) 196, 358
AjnI CCWGG 1 cut(s) 568
AleI CACNNNNGTG 1 cut(s) 473
AluBI AGCT 4 cut(s) 56, 106, 202, 382
AluI AGCT 4 cut(s) 56, 106, 202, 382
Alw21I GWGCWC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 385
AoxI GGCC 3 cut(s) 207, 217, 532
ApaI GGGCCC 1 cut(s) 536
ApeKI GCWGC 2 cut(s) 379, 382
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 3 cut(s) 455, 532, 533
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 3 cut(s) 53, 121, 487
AvaII GGWCC 1 cut(s) 455
BaeGI GKGCMC 1 cut(s) 536
BaeI ACNNNNGTAYC 2 cut(s) 407, 440
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 2 cut(s) 423, 467
BanII GRGCYC 1 cut(s) 536
BarI GAAGNNNNNNTAC 2 cut(s) 40, 72
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 369, 391
BccI CCATC 1 cut(s) 288
BciT130I CCWGG 1 cut(s) 570
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 1 cut(s) 531
BfrI CTTAAG 1 cut(s) 428
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 380, 383
BlsI GCNGC 2 cut(s) 381, 384
Bme1390I CCNGG 2 cut(s) 531, 570
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 455, 532, 533
BmiI GGNNCC 4 cut(s) 425, 456, 469, 534
BmrFI CCNGG 2 cut(s) 531, 570
BmrI ACTGGG 2 cut(s) 8, 100
BmsI GCATC 2 cut(s) 67, 533
BmuI ACTGGG 2 cut(s) 8, 100
BoxI GACNNNNGTC 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 242, 302
BpuMI CCSGG 1 cut(s) 531
BsaJI CCNNGG 1 cut(s) 471
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 514
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 3 cut(s) 14, 95, 417
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 464, 514
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 3 cut(s) 14, 95, 417
BseSI GKGCMC 1 cut(s) 536
BseXI GCAGC 2 cut(s) 369, 391
BshFI GGCC 3 cut(s) 209, 219, 534
BshNI GGYRCC 2 cut(s) 423, 467
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 2 cut(s) 530, 547
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 2 cut(s) 464, 514
BsmFI GGGAC 1 cut(s) 468
BsnI GGCC 3 cut(s) 209, 219, 534
Bsp120I GGGCCC 1 cut(s) 532
Bsp1286I GDGCHC 2 cut(s) 168, 536
Bsp143I GATC 3 cut(s) 69, 313, 510
BspACI CCGC 1 cut(s) 550
BspANI GGCC 3 cut(s) 209, 219, 534
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 4 cut(s) 425, 456, 469, 534
BspQI GCTCTTC 1 cut(s) 570
BspT107I GGYRCC 2 cut(s) 423, 467
BspTI CTTAAG 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 3 cut(s) 14, 95, 417
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 3 cut(s) 69, 313, 510
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 570
BstAFI CTTAAG 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 211, 548
BstDEI CTNAG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 1 cut(s) 328
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 3 cut(s) 72, 316, 513
BstMBI GATC 3 cut(s) 69, 313, 510
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 570
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 529, 568
BstSLI GKGCMC 1 cut(s) 536
BstV1I GCAGC 2 cut(s) 369, 391
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuRI GGCC 3 cut(s) 209, 219, 534
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 150, 182
Cac8I GCNNGC 2 cut(s) 211, 548
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 3 cut(s) 455, 532, 533
Csp6I GTAC 3 cut(s) 308, 328, 424
CviAII CATG 2 cut(s) 116, 492
CviJI RGCY 8 cut(s) 33, 56, 106, 202, 209, 219, 382, 534
CviKI_1 RGCY 8 cut(s) 33, 56, 106, 202, 209, 219, 382, 534
CviQI GTAC 3 cut(s) 308, 328, 424
DdeI CTNAG 1 cut(s) 129
DpnI GATC 3 cut(s) 71, 315, 512
DpnII GATC 3 cut(s) 69, 313, 510
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EciI GGCGGA 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 536
Eco47I GGWCC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 2 cut(s) 119, 495
FaiI YATR 4 cut(s) 117, 251, 444, 493
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 1 cut(s) 468
FatI CATG 2 cut(s) 115, 491
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 2 cut(s) 380, 383
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 380, 383
GlaI GCGC 1 cut(s) 278
GluI GCNGC 2 cut(s) 380, 383
HaeIII GGCC 3 cut(s) 209, 219, 534
HapII CCGG 2 cut(s) 530, 547
HhaI GCGC 1 cut(s) 279
Hin1II CATG 2 cut(s) 119, 495
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 5 cut(s) 112, 121, 192, 403, 553
HpaII CCGG 2 cut(s) 530, 547
HphI GGTGA 3 cut(s) 53, 121, 487
Hpy166II GTNNAC 2 cut(s) 156, 521
Hpy188I TCNGA 2 cut(s) 234, 304
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 2 cut(s) 156, 521
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 2 cut(s) 119, 495
HspAI GCGC 1 cut(s) 277
KpnI GGTACC 1 cut(s) 427
KroI GCCGGC 1 cut(s) 546
KroNI GCCGGC 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 3 cut(s) 69, 313, 510
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 2 cut(s) 184, 199
Lsp1109I GCAGC 2 cut(s) 369, 391
LweI GCATC 2 cut(s) 67, 533
MalI GATC 3 cut(s) 71, 315, 512
MboI GATC 3 cut(s) 69, 313, 510
MboII GAAGA 3 cut(s) 60, 275, 557
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 168, 536
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 2 cut(s) 359, 368
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 121, 547
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 6 cut(s) 37, 44, 144, 217, 297, 498
Mox20I TGGCCA 1 cut(s) 209
MroNI GCCGGC 1 cut(s) 546
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 2 cut(s) 473, 541
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 382
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 530, 547
MspR9I CCNGG 2 cut(s) 531, 570
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 1 cut(s) 172
NaeI GCCGGC 1 cut(s) 548
NciI CCSGG 1 cut(s) 531
NdeII GATC 3 cut(s) 69, 313, 510
NgoMIV GCCGGC 1 cut(s) 546
NlaIII CATG 2 cut(s) 119, 495
NlaIV GGNNCC 4 cut(s) 425, 456, 469, 534
OliI CACNNNNGTG 1 cut(s) 473
PceI AGGCCT 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 570
PdiI GCCGGC 1 cut(s) 548
PfeI GAWTC 3 cut(s) 121, 192, 403
PflMI CCANNNNNTGG 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 381, 384
PleI GAGTC 2 cut(s) 120, 547
PpsI GAGTC 2 cut(s) 120, 547
PshAI GACNNNNGTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 4 cut(s) 425, 456, 469, 534
PspOMI GGGCCC 1 cut(s) 532
PspPI GGNCC 3 cut(s) 455, 532, 533
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 3 cut(s) 309, 329, 425
RsaNI GTAC 3 cut(s) 308, 328, 424
RseI CAYNNNNRTG 2 cut(s) 473, 541
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 2 cut(s) 380, 383
Sau3AI GATC 3 cut(s) 69, 313, 510
Sau96I GGNCC 3 cut(s) 455, 532, 533
SchI GAGTC 2 cut(s) 121, 547
ScrFI CCNGG 2 cut(s) 531, 570
SduI GDGCHC 2 cut(s) 168, 536
SetI ASST 8 cut(s) 58, 108, 155, 204, 384, 429, 477, 510
SfaNI GCATC 2 cut(s) 67, 533
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 2 cut(s) 473, 541
SmlI CTYRAG 3 cut(s) 221, 317, 428
SmoI CTYRAG 3 cut(s) 221, 317, 428
Sse9I AATT 2 cut(s) 359, 368
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 1 cut(s) 550
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 529, 568
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 145
TaqI TCGA 1 cut(s) 293
TasI AATT 2 cut(s) 359, 368
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 3 cut(s) 121, 192, 403
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 2 cut(s) 150, 182
TseI GCWGC 2 cut(s) 379, 382
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 2 cut(s) 150, 182
Van91I CCANNNNNTGG 1 cut(s) 464
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 455
XagI CCTNNNNNAGG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.