pycom09g06950

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0023564
Physical Location & Seq
Forward (+)
5151028 .. 5152810
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGGCGAGCTCCAGCGGTACGAAGAACGAGTTCCGGGGGCCGTCTCGGTCATTGTCGAGGGCAATGACGAGAATGCCCACAAGGGTTGTGGAGTTGCCGAACGAGGACGAGTCGTTGGACAACATAACTGTTCCCTCGGGACTTCCCTCAATTGCTCCGGTTTTTCGAGTGGCGGATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAGAGGGCACACAAGATGGATCCAACATCAAGTGGACGTGGTGTTCGACAGTTTAAGACACTTCTTTTGAACAAACTTGAAAAGGACGAAATATTGACAAAACATCAGCTTGCTAGATCTGATCGACTATAA

Protein Analysis

121

Amino Acids

13.55

Weight (kDa)

9.57

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 106 2.6e-09 Vta1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14780
malus_domestica MD09G1150100.v1.1
pyrus_communis pycom09g06950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 15, 173, 217
AclWI GGATC 2 cut(s) 245, 258
AfaI GTAC 1 cut(s) 19
AgsI TTSAA 2 cut(s) 301, 311
AjiI CACGTC 1 cut(s) 269
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 2 cut(s) 9, 340
AluI AGCT 2 cut(s) 9, 340
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 2 cut(s) 245, 258
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 1 cut(s) 38
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
Asp700I GAANNNNTTC 1 cut(s) 29
AspA2I CCTAGG 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 136
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 241
BamHI GGATCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 11
BccI CCATC 1 cut(s) 241
BceAI ACGGC 1 cut(s) 25
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 2 cut(s) 198, 345
BglII AGATCT 1 cut(s) 347
BisI GCNGC 1 cut(s) 217
BlnI CCTAGG 1 cut(s) 197
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 2 cut(s) 39, 252
BmrFI CCNGG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 35
BsaJI CCNNGG 4 cut(s) 34, 135, 197, 223
BsaWI WCCGGW 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 69
BseDI CCNNGG 4 cut(s) 34, 135, 197, 223
BseGI GGATG 1 cut(s) 181
BseMI GCAATG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 241
BshFI GGCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 34, 158
BslFI GGGAC 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 11, 241
Bsp143I GATC 3 cut(s) 250, 347, 352
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 3 cut(s) 15, 173, 217
BspANI GGCC 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 39, 252
BspPI GGATC 2 cut(s) 245, 258
BsrDI GCAATG 1 cut(s) 69
BssECI CCNNGG 4 cut(s) 34, 135, 197, 223
BssMI GATC 3 cut(s) 250, 347, 352
BssT1I CCWWGG 2 cut(s) 197, 223
Bst4CI ACNGT 2 cut(s) 130, 282
BstC8I GCNNGC 2 cut(s) 7, 342
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 181
BstKTI GATC 3 cut(s) 253, 350, 355
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 3 cut(s) 250, 347, 352
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 241
BstX2I RGATCY 2 cut(s) 250, 347
BstYI RGATCY 2 cut(s) 250, 347
BsuRI GGCC 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 181
Cac8I GCNNGC 2 cut(s) 7, 342
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 18
CspCI CAANNNNNGTGG 4 cut(s) 67, 69, 102, 104
CviAII CATG 1 cut(s) 224
CviJI RGCY 3 cut(s) 9, 40, 340
CviKI_1 RGCY 3 cut(s) 9, 40, 340
CviQI GTAC 1 cut(s) 18
DpnI GATC 3 cut(s) 252, 349, 354
DpnII GATC 3 cut(s) 250, 347, 352
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 197, 223
Eco24I GRGCYC 1 cut(s) 11
Eco53kI GAGCTC 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 9
EcoT14I CCWWGG 2 cut(s) 197, 223
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 197, 223
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 1 cut(s) 227
FaiI YATR 3 cut(s) 125, 225, 361
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 223
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 217
FspBI CTAG 2 cut(s) 198, 345
GluI GCNGC 1 cut(s) 217
HaeIII GGCC 1 cut(s) 40
HapII CCGG 2 cut(s) 34, 158
Hin1II CATG 1 cut(s) 227
HinfI GANTC 1 cut(s) 110
HpaII CCGG 2 cut(s) 34, 158
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 1 cut(s) 138
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 2 cut(s) 130, 282
HpyCH4IV ACGT 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 268
Hsp92II CATG 1 cut(s) 227
Kzo9I GATC 3 cut(s) 250, 347, 352
LmnI GCTCC 2 cut(s) 14, 160
LpnPI CCDG 3 cut(s) 25, 47, 171
MaeI CTAG 2 cut(s) 198, 345
MaeII ACGT 1 cut(s) 268
MalI GATC 3 cut(s) 252, 349, 354
MboI GATC 3 cut(s) 250, 347, 352
MboII GAAGA 1 cut(s) 34
MfeI CAATTG 1 cut(s) 150
MflI RGATCY 2 cut(s) 250, 347
MhlI GDGCHC 2 cut(s) 11, 241
MluCI AATT 1 cut(s) 150
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 96, 278
MnlI CCTC 5 cut(s) 51, 97, 145, 157, 228
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 2 cut(s) 34, 158
MspR9I CCNGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 78
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 223
NdeII GATC 3 cut(s) 250, 347, 352
NlaIII CATG 1 cut(s) 227
NlaIV GGNNCC 2 cut(s) 39, 252
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 218
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 39, 252
PspPI GGNCC 1 cut(s) 38
PsuI RGATCY 2 cut(s) 250, 347
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 3 cut(s) 250, 347, 352
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 11, 241
SetI ASST 3 cut(s) 11, 271, 342
Sse9I AATT 1 cut(s) 150
SsiI CCGC 3 cut(s) 15, 173, 217
SspI AATATT 1 cut(s) 324
SspMI CTAG 2 cut(s) 198, 345
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 2 cut(s) 197, 223
TaaI ACNGT 2 cut(s) 130, 282
TaiI ACGT 1 cut(s) 271
TaqI TCGA 4 cut(s) 56, 166, 277, 355
TaqII GACCGA 1 cut(s) 36
TasI AATT 1 cut(s) 150
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmaJI CCTAGG 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 2 cut(s) 198, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.