pycom10g00280

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029373
Physical Location & Seq
Forward (+)
375254 .. 375694
441 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 294 bp
ATGAACAAAGCCAAGGCAATTAAGGTGTTGTGTGCAAAGCCTTATGAGATAGAGAGGTCTAGACCTCACGCCAATCGAACAAGACTCCGATCTCAACAGATTGGTAGTAGGGATACATCTGAACGCATCCGTACATACACTTTTTTCCAAGGACGTGTAACTGATCACCGTGTTGGAATCACACATCATTCGATAAATGATGTGATGCAGGGAAAGAATCTGGATGTATTTATTGATGCCCTTCCTTTGCAAGAAGAAATGGATGCAATTGCTTCTTTCAGTTGTTCGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

98

Amino Acids

11.13

Weight (kDa)

9.44

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 2 - 57 1.3e-08 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029373)

Species Orthologous Gene IDs
pyrus_communis pycom10g00280
rosa_roxburghii Rroxscaffold_2G00118230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 133
AflIII ACRYGT 1 cut(s) 154
AjiI CACGTC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 158
BciVI GTATCC 1 cut(s) 106
BclI TGATCA 1 cut(s) 163
BfaI CTAG 1 cut(s) 60
BfuI GTATCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 155
BmsI GCATC 4 cut(s) 135, 195, 226, 253
BsaJI CCNNGG 2 cut(s) 12, 148
BseDI CCNNGG 2 cut(s) 12, 148
BseGI GGATG 3 cut(s) 126, 229, 268
Bsp143I GATC 2 cut(s) 89, 163
BssECI CCNNGG 2 cut(s) 12, 148
BssMI GATC 2 cut(s) 89, 163
BssT1I CCWWGG 2 cut(s) 12, 148
Bst4CI ACNGT 1 cut(s) 170
BstF5I GGATG 3 cut(s) 126, 229, 268
BstKTI GATC 2 cut(s) 92, 166
BstMBI GATC 2 cut(s) 89, 163
BsuI GTATCC 1 cut(s) 106
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 3 cut(s) 126, 229, 268
Csp6I GTAC 1 cut(s) 132
CviJI RGCY 2 cut(s) 11, 40
CviKI_1 RGCY 2 cut(s) 11, 40
CviQI GTAC 1 cut(s) 132
DpnI GATC 2 cut(s) 91, 165
DpnII GATC 2 cut(s) 89, 163
Eco130I CCWWGG 2 cut(s) 12, 148
EcoT14I CCWWGG 2 cut(s) 12, 148
ErhI CCWWGG 2 cut(s) 12, 148
FaiI YATR 2 cut(s) 45, 136
FbaI TGATCA 1 cut(s) 163
FokI GGATG 3 cut(s) 113, 236, 275
FspBI CTAG 1 cut(s) 60
HinfI GANTC 3 cut(s) 84, 177, 217
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 2 cut(s) 89, 121
Hpy188III TCNNGA 2 cut(s) 60, 221
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 4 cut(s) 35, 208, 250, 266
HpySE526I ACGT 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 2 cut(s) 89, 163
LpnPI CCDG 2 cut(s) 194, 206
LweI GCATC 4 cut(s) 135, 195, 226, 253
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 157
MalI GATC 2 cut(s) 91, 165
MboI GATC 2 cut(s) 89, 163
MboII GAAGA 1 cut(s) 266
MfeI CAATTG 1 cut(s) 267
MluCI AATT 2 cut(s) 18, 267
MlyI GAGTC 1 cut(s) 78
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 2 cut(s) 48, 75
MseI TTAA 1 cut(s) 21
MunI CAATTG 1 cut(s) 267
NdeII GATC 2 cut(s) 89, 163
PfeI GAWTC 2 cut(s) 177, 217
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 21
Sau3AI GATC 2 cut(s) 89, 163
SchI GAGTC 1 cut(s) 78
SetI ASST 4 cut(s) 27, 59, 67, 157
SfaNI GCATC 4 cut(s) 135, 195, 226, 253
Sse9I AATT 2 cut(s) 18, 267
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 2 cut(s) 12, 148
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 1 cut(s) 157
TaqI TCGA 2 cut(s) 76, 191
TasI AATT 2 cut(s) 18, 267
TfiI GAWTC 2 cut(s) 177, 217
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 119
XbaI TCTAGA 1 cut(s) 59
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.