Rroxscaffold_2G00118230

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0029373
Physical Location & Seq
Forward (+)
49472985 .. 49474122
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118230.1

Sequence Viewer

Length: 456 bp
ATGGTCGCTGCCATGGTTTACCAGCAAGATGGGGAAGGCATGGCTACCATGTCTACCAAGACTGGTGGAATTGGGTCATGTCAGAGGATTGAAGGGAGAAAGAGAGAAACAGAAGGGAGGAATGGAGAGACAAAGTGGACGAGAAGGGGTGCTCCTATTTCTGCTAACAAGGCCAAGGCACTTAAAGTACTGTGTGCAAAGCTATATGAGATGGAAAGGTCTAGACTACAGAACAATCGGTCAAAACTTCGATTAGAGCAGATTGGCAGTGGGGATCGATCAGAACGCATTCGTACATACAACTTTCCTCAAGGTCGTGTAACTGATCATCGTGTTGGCATTACTCATCATGCAATAAGTGATGTGATGCAGGGAGAGAATCTGGATGTGTTTGTTGATGCGCTTCTTTTGCAGCAGGAAATGGATGCAATCGCATCTTTCAGTTCTTCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

151

Amino Acids

16.86

Weight (kDa)

9.46

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 55 - 111 3.6e-12 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029373)

Species Orthologous Gene IDs
pyrus_communis pycom10g00280
rosa_roxburghii Rroxscaffold_2G00118230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 53
AclWI GGATC 1 cut(s) 282
AfaI GTAC 2 cut(s) 189, 295
AgsI TTSAA 1 cut(s) 92
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 1 cut(s) 282
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 2 cut(s) 8, 412
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 424
BccI CCATC 2 cut(s) 23, 205
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 227
BisI GCNGC 2 cut(s) 9, 413
BlsI GCNGC 2 cut(s) 10, 414
BmcAI AGTACT 1 cut(s) 189
BmsI GCATC 4 cut(s) 357, 388, 415, 443
BpuEI CTTGAG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 277
BsaJI CCNNGG 2 cut(s) 12, 174
Bse1I ACTGG 1 cut(s) 67
BseCI ATCGAT 1 cut(s) 277
BseDI CCNNGG 2 cut(s) 12, 174
BseGI GGATG 2 cut(s) 391, 430
BseNI ACTGG 1 cut(s) 67
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 1 cut(s) 173
BshVI ATCGAT 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 154
Bsp143I GATC 3 cut(s) 274, 278, 325
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 1 cut(s) 173
BspDI ATCGAT 1 cut(s) 277
BspPI GGATC 1 cut(s) 282
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 12, 174
BssMI GATC 3 cut(s) 274, 278, 325
BssT1I CCWWGG 2 cut(s) 12, 174
Bst4CI ACNGT 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 391, 430
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 3 cut(s) 277, 281, 328
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 3 cut(s) 274, 278, 325
BstMWI GCNNNNNNNGC 2 cut(s) 170, 409
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 277
BsuRI GGCC 1 cut(s) 173
BsuTUI ATCGAT 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 391, 430
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
CfoI GCGC 1 cut(s) 403
ClaI ATCGAT 1 cut(s) 277
Csp6I GTAC 2 cut(s) 188, 294
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 5 cut(s) 13, 40, 49, 78, 350
CviJI RGCY 3 cut(s) 44, 173, 202
CviKI_1 RGCY 3 cut(s) 44, 173, 202
CviQI GTAC 2 cut(s) 188, 294
DpnI GATC 3 cut(s) 276, 280, 327
DpnII GATC 3 cut(s) 274, 278, 325
Eco130I CCWWGG 2 cut(s) 12, 174
EcoT14I CCWWGG 2 cut(s) 12, 174
ErhI CCWWGG 2 cut(s) 12, 174
FaeI CATG 5 cut(s) 16, 43, 52, 81, 353
FaiI YATR 8 cut(s) 14, 41, 50, 79, 205, 207, 298, 351
FatI CATG 5 cut(s) 12, 39, 48, 77, 349
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 53
Fnu4HI GCNGC 2 cut(s) 9, 413
FokI GGATG 2 cut(s) 398, 437
Fsp4HI GCNGC 2 cut(s) 9, 413
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 402
GluI GCNGC 2 cut(s) 9, 413
HaeIII GGCC 1 cut(s) 173
HhaI GCGC 1 cut(s) 403
Hin1II CATG 5 cut(s) 16, 43, 52, 81, 353
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HinfI GANTC 1 cut(s) 379
Hpy166II GTNNAC 3 cut(s) 19, 54, 138
Hpy188I TCNGA 2 cut(s) 84, 283
Hpy188III TCNNGA 2 cut(s) 222, 383
Hpy8I GTNNAC 3 cut(s) 19, 54, 138
HpyAV CCTTC 4 cut(s) 29, 86, 107, 138
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4V TGCA 5 cut(s) 197, 353, 370, 412, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 409
Hsp92II CATG 5 cut(s) 16, 43, 52, 81, 353
HspAI GCGC 1 cut(s) 401
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 274, 278, 325
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 5 cut(s) 35, 48, 356, 368, 401
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 4 cut(s) 357, 388, 415, 443
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 319
MalI GATC 3 cut(s) 276, 280, 327
MboI GATC 3 cut(s) 274, 278, 325
MboII GAAGA 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 1 cut(s) 69
MnlI CCTC 3 cut(s) 78, 111, 318
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 170, 409
NcoI CCATGG 1 cut(s) 12
NdeII GATC 3 cut(s) 274, 278, 325
NlaIII CATG 5 cut(s) 16, 43, 52, 81, 353
PctI GAATGC 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 2 cut(s) 10, 414
RsaI GTAC 2 cut(s) 189, 295
RsaNI GTAC 2 cut(s) 188, 294
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 2 cut(s) 9, 413
Sau3AI GATC 3 cut(s) 274, 278, 325
ScaI AGTACT 1 cut(s) 189
SduI GDGCHC 1 cut(s) 154
SetI ASST 3 cut(s) 204, 221, 316
SfaNI GCATC 4 cut(s) 357, 388, 415, 443
SfcI CTRYAG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
Sse9I AATT 1 cut(s) 69
SspMI CTAG 1 cut(s) 222
StyI CCWWGG 2 cut(s) 12, 174
TaaI ACNGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 250, 277
TaqII GACCGA 1 cut(s) 228
TasI AATT 1 cut(s) 69
TatI WGTACW 1 cut(s) 187
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 274
TseI GCWGC 2 cut(s) 8, 412
TspRI CASTG 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 222
ZrmI AGTACT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.