Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0020965

Physical Location & Seq

Genomic Coordinates Forward (+)

17051707 .. 17054412

Gene Structure 2706 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2706 bp

ATGTTGGTTCGCACATACAATGTCCGAAACGTCCTTCTGAGAGCTCCCTATTTGTTCGAACTTCCTCCGACGCTTTCCTCCGTCGCATACCAATCTTCCGGCAGCCGAGCAGCCCCAGAAAGCTCAGTTGACACGACTTCCCGCGTATCGTCTCTGATTCAGCAACCCAATTGGGAAAGGAGCAGTCTTCTCAAGTCTATGGTCTCCCACATGGCACCACAAGTAGCCGCCAAGGTTTTACAGCTCCATGGCGGCGACACCGAACTCGGGGTTCGCTTTTTCAAGTGGGTTTGTAAGCATTCTACTTATTGTTATGATTTGGATAGCAGAATCGCTCTGTTGAATTTGTTGGTTACTTGTGAAAGTTTATATGGGGTTGCTCAGAAAGCCATTGTTTTGTTAATCAAGGAGTTAAACAATAGTGAGCCAGAGATTACGAGGCTGATGGGGGCGTTTGAAAATATGCGAAAGGTTGGATTTTGGTTGAGTTATCCTTGTTATAGTAGCCTTTTGATGTCTTTAGCTAGGTTGGATTTGGGGTTTTTGACTTTTTTGGTGTATAAAAAGATGGTTAGTGATGGGTTTCTTCTTGGTGTTATTGACTACAGAAGTGTAATTAATGCTCTATGCAAAAATGGGTTTGTGCAATCTGCTGAAATGTTTGTGTGTAGGGTTTTGAAACTCGGGTTTCGGTTGGACACTCATATTTGTACTTCTTTGGTGTTGGGTAATTGTAGAGAGAGTCATCTCCAGGAGGCCTCCCGGGTGTTCGATATAATGTCTAAGAGTCATGGTTGTGAGCCTAATTCGGTGACGTACTCGATTTTGGTTCATGGTTACTGTGAAATTGATAAGCTTGATGAAGCTTTTCGTCTGAAAGAAGAGATGAGTGAGAAGGGCTGCCAGCCGAGTAGTCGTACGTTTACAGTACTCATCAAGGCTTTGTGTGACATTGGGTCGACTGATAAGGCTTTAGGGTTGCTTGATGAGATGGTTACAAAGGGTTGTAAACCGAATGTTCATACTTATACCATATTGATTGATAGATTATGCAGGGAAGGAAAGATTAAGGAGGCGAATGGAATGTTCAGAAAGATGCTTGCAGATGAGCTTTTTCCTGGCACAGTAACTTACAATGCACTGATAAATGGATATTGCAAAGAAGGACGCGTAATTTCTGCTTTTGAGCTTCTTGGTGTGATGGAGAAAAGACTATGTAAGCCTAACATTCGGACCTACAATGAGCTCATCGAAGGGCTATGCAAGGTTTATAAAACTTACAAAGCCATGTTTCTTTTGAAAAGAATAGTTGATAATGGTTTGTTACCAAATAGGGTTACCTACAATATTTTGATCGATGGGTTTTGCAAAGAAGGTCAACTTGGATTGGCTTTTGAAACGTTTAAGTCAATGAGCTCGTTCGGTATTGAACCAGATTGTTTTTCTTTCACGGCCTTGATTGATGGCCTCTGTAAGCAAGGGAGACCAGGGCATGCAAGCAGCATTTTAGGTTTAATGGTAAAGAAGGGAATTTCCCCTGATGAAGTAACTATGACAGCTCTTATTGACGGGTATTGCAAGAATGACGAAATTGGAAACGCATCGATGCTTTTTGAGAAGATGGTGGAGAAGAAGACATTGACAACACCACACACTTTCAATTCTTTTCTTGATGTCCTCAGCAAAGATGATAAGGTGCATGCGGCACAGGCAATGTTCGGGAAGATGCTGAAGTATGGATCAGTTCCATCTGTAGTGACTTATACAATATTAGTCGATGGGCTTTGCCAAACAGGTGATATTATGTGTGCACTAAAAATGTTGGATTTGATGAGGCGAACTAGCCGCCCTCCAAATGTCTACACCTACACAGTCGTGATCAATGGTCTCTGCCTGAACGGAAGAGTGGAGGAAGCAGAAAAGCTTCTCTTTAGCATGTCTGATTTCGGTATACCTCCAAACCACGTTACATATACTGTATTAGTTAAAGGTCTTGTTAATGCTGGTAGGTTGGATCATGCCTTTGAGATTTTGAGTGTTATGGTTCAAAAAGGTTTTCAGCCAAACCCTCGAATCTATTCTGCGCTTCTTGCAGGTCTTGTTTTGTCAAACGAGGCCAAGGAAGATGCCAGTTCATTTATGTCTTCCACTTATGCTACACCATTACTTCCGAAGGACATTGACGACAATTGCATTTCTAGCTACGCTTTTAGAAATATGGATATTGAACATGCTTTCAGTCTTGAAGAAAAGGTCAGAAAATGTGGCTGCTCTACAATGGATTTGTACAACTTTGTTGTCATGGGATTATGCAGGGAAGCAAGGGTTGCAGAAGCAGATCATATCATTAACAATGTACTAAAATGCGGCGTGTTTCCTCAGAAGGCTGTTTGTGCACTCATCAACAGTTATTGCAACGAGCGCCGATATGATCATTGCCTCGATTTCATGACAACAATTCTGAATCAAGGGTTTGTTCCATCCGTGTTATCATATTGTTCGGTGATTCAGGGACTTTACAGTGAAGGGAGGGCTGAACAAGGTGAAGAACTTTTCTCTGAACTATTAAGACACAATGATATCAAGGAAAAGTCCTCAGTTTTGCCATATTTGGAGATCCTAGTAAAGAAAGAGGAACCTGAGGATTGCCTTCACATTCTCAAACTAATTGAGCAAATGGGTTGCACAGAGAGGCCAATTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

902

Amino Acids

100.87

Weight (kDa)

6.97

Isoelectric Point (pI)

37.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	233 - 281	5.6e-11	PPR repeat family
PPR_long	PF17177	236 - 353	3.8e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	253 - 365	1.1e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	264 - 297	2.3e-12	PPR repeat
PPR_3	PF13812	265 - 315	1.2e-06	Pentatricopeptide repeat domain
PPR	PF01535	271 - 301	3.8e-06	PPR repeat
PPR_2	PF13041	276 - 317	1.2e-12	PPR repeat family
PPR_1	PF12854	299 - 332	1.8e-08	PPR repeat
PPR_2	PF13041	311 - 352	2.1e-13	PPR repeat family
PPR_3	PF13812	327 - 386	6.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	334 - 366	1.1e-12	PPR repeat
PPR_3	PF13812	363 - 418	7.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	371 - 402	2.6e-11	PPR repeat
PPR_2	PF13041	373 - 422	1.7e-17	PPR repeat family
TPR_24	PF23276	375 - 471	3.2e-06	Fungal tetratrico peptide repeats
PPR	PF01535	376 - 404	6.5e-08	PPR repeat
PPR_3	PF13812	432 - 488	3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	439 - 471	5.6e-12	PPR repeat
PPR_2	PF13041	443 - 492	2.4e-17	PPR repeat family
PPR	PF01535	446 - 476	1.3e-07	PPR repeat
PPR_1	PF12854	475 - 506	3.5e-07	PPR repeat
PPR_2	PF13041	482 - 527	7.8e-13	PPR repeat family
PPR_1	PF12854	509 - 542	3e-08	PPR repeat
PPR_2	PF13041	513 - 562	4.7e-10	PPR repeat family
PPR_2	PF13041	552 - 597	7e-11	PPR repeat family
PPR_1	PF12854	580 - 612	8.1e-10	PPR repeat
PPR_2	PF13041	583 - 631	6.1e-15	PPR repeat family
PPR_long	PF17177	595 - 706	8.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	618 - 646	3.6e-10	PPR repeat
PPR_2	PF13041	618 - 667	8.6e-15	PPR repeat family
PPR	PF01535	621 - 651	6e-07	PPR repeat
PPR_3	PF13812	642 - 697	4.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	650 - 682	6.8e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020965)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07290
malus_domestica	MD10G1157300.v1.1
prunus_persica	Prupe.8G196100_v2.0.a1 Prupe.8G196100_v2.0.a1
pyrus_communis	pycom10g13580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1272
Acc36I	ACCTGC	1 cut(s)	2084
AccB1I	GGYRCC	1 cut(s)	214
AccI	GTMKAC	3 cut(s)	959, 1860, 1951
AccII	CGCG	2 cut(s)	144, 1170
AciI	CCGC	6 cut(s)	142, 228, 252, 1703, 1846, 2367
AclI	AACGTT	1 cut(s)	1400
AclWI	GGATC	3 cut(s)	1747, 2022, 2611
AcsI	RAATTY	2 cut(s)	343, 1530
AcuI	CTGAAG	1 cut(s)	1751
AfaI	GTAC	6 cut(s)	712, 818, 919, 930, 2288, 2358
AfiI	CCNNNNNNNGG	1 cut(s)	267
AflIII	ACRYGT	1 cut(s)	1168
AhdI	GACNNNNNGTC	1 cut(s)	955
AjnI	CCWGG	3 cut(s)	750, 1117, 1486
AjuI	GAANNNNNNNTTGG	2 cut(s)	1365, 1397
AleI	CACNNNNGTG	1 cut(s)	1874
Alw21I	GWGCWC	5 cut(s)	46, 1248, 1418, 1813, 2398
Alw26I	GTCTC	4 cut(s)	156, 208, 1477, 1892
Alw44I	GTGCAC	2 cut(s)	1809, 2394
AlwI	GGATC	3 cut(s)	1747, 2022, 2611
Ama87I	CYCGRG	3 cut(s)	266, 683, 762
AoxI	GGCC	5 cut(s)	756, 1452, 1465, 2115, 2693
ApaLI	GTGCAC	2 cut(s)	1809, 2394
ApeKI	GCWGC	5 cut(s)	102, 110, 900, 1500, 2268
ApoI	RAATTY	2 cut(s)	343, 1530
AseI	ATTAAT	1 cut(s)	618
Asp700I	GAANNNNTTC	3 cut(s)	867, 1923, 2077
AspLEI	GCGC	2 cut(s)	2086, 2424
AspS9I	GGNCC	1 cut(s)	1233
AsuC2I	CCSGG	2 cut(s)	763, 764
AsuHPI	GGTGA	4 cut(s)	823, 1808, 2515, 2555
AsuII	TTCGAA	1 cut(s)	57
AvaI	CYCGRG	3 cut(s)	266, 683, 762
AvaII	GGWCC	1 cut(s)	1233
AxyI	CCTNAGG	1 cut(s)	2640
BaeGI	GKGCMC	2 cut(s)	1813, 2398
BanI	GGYRCC	1 cut(s)	214
BanII	GRGCYC	3 cut(s)	46, 1248, 1418
BbsI	GAAGAC	3 cut(s)	179, 1640, 2136
Bbv12I	GWGCWC	5 cut(s)	46, 1248, 1418, 1813, 2398
BbvCI	CCTCAGC	1 cut(s)	1679
BbvI	GCAGC	5 cut(s)	114, 122, 887, 1512, 2255
BceAI	ACGGC	1 cut(s)	1467
BciT130I	CCWGG	3 cut(s)	752, 1119, 1488
BclI	TGATCA	2 cut(s)	1878, 2431
BcnI	CCSGG	2 cut(s)	763, 764
BcoDI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BfaI	CTAG	4 cut(s)	525, 1842, 2199, 2621
BfmI	CTRYAG	2 cut(s)	604, 1752
BfoI	RGCGCY	1 cut(s)	2425
BfuAI	ACCTGC	1 cut(s)	2084
BmcAI	AGTACT	1 cut(s)	930
Bme1390I	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
Bme18I	GGWCC	1 cut(s)	1233
BmeRI	GACNNNNNGTC	1 cut(s)	955
BmeT110I	CYCGRG	3 cut(s)	266, 683, 762
BmgT120I	GGNCC	1 cut(s)	1233
BmiI	GGNNCC	2 cut(s)	216, 2637
BmrFI	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
BmsI	GCATC	5 cut(s)	1086, 1596, 1610, 1716, 2116
BpiI	GAAGAC	3 cut(s)	179, 1640, 2136
BpmI	CTGGAG	1 cut(s)	734
Bpu10I	CCTNAGC	1 cut(s)	1679
Bpu14I	TTCGAA	1 cut(s)	57
BpuEI	CTTGAG	1 cut(s)	176
BpuMI	CCSGG	2 cut(s)	763, 764
Bsa29I	ATCGAT	2 cut(s)	1356, 1604
BsaI	GGTCTC	3 cut(s)	208, 1477, 1892
BsaJI	CCNNGG	5 cut(s)	231, 247, 762, 1487, 2118
Bsc4I	CCNNNNNNNGG	1 cut(s)	267
Bse1I	ACTGG	1 cut(s)	2130
Bse21I	CCTNAGG	1 cut(s)	2640
Bse3DI	GCAATG	2 cut(s)	1719, 2434
BseBI	CCWGG	3 cut(s)	752, 1119, 1488
BseCI	ATCGAT	2 cut(s)	1356, 1604
BseDI	CCNNGG	5 cut(s)	231, 247, 762, 1487, 2118
BseGI	GGATG	1 cut(s)	2480
BseLI	CCNNNNNNNGG	1 cut(s)	267
BseMI	GCAATG	2 cut(s)	1719, 2434
BseMII	CTCAG	7 cut(s)	29, 138, 395, 1693, 2393, 2610, 2631
BseNI	ACTGG	1 cut(s)	2130
BseSI	GKGCMC	2 cut(s)	1813, 2398
BseXI	GCAGC	5 cut(s)	114, 122, 887, 1512, 2255
Bsh1236I	CGCG	2 cut(s)	144, 1170
BshFI	GGCC	5 cut(s)	758, 1454, 1467, 2117, 2695
BshNI	GGYRCC	1 cut(s)	214
BshVI	ATCGAT	2 cut(s)	1356, 1604
BsiHKAI	GWGCWC	5 cut(s)	46, 1248, 1418, 1813, 2398
BsiHKCI	CYCGRG	3 cut(s)	266, 683, 762
BsiSI	CCGG	2 cut(s)	99, 763
BsiWI	CGTACG	1 cut(s)	917
BslFI	GGGAC	1 cut(s)	2526
BslI	CCNNNNNNNGG	1 cut(s)	267
BsmAI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BsmBI	CGTCTC	1 cut(s)	156
BsmFI	GGGAC	1 cut(s)	2526
BsmI	GAATGC	1 cut(s)	298
BsnI	GGCC	5 cut(s)	758, 1454, 1467, 2117, 2695
Bso31I	GGTCTC	3 cut(s)	208, 1477, 1892
BsoBI	CYCGRG	3 cut(s)	266, 683, 762
Bsp119I	TTCGAA	1 cut(s)	57
Bsp1286I	GDGCHC	5 cut(s)	46, 1248, 1418, 1813, 2398
Bsp1407I	TGTACA	1 cut(s)	2286
Bsp143I	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
Bsp19I	CCATGG	1 cut(s)	247
BspACI	CCGC	6 cut(s)	142, 228, 252, 1703, 1846, 2367
BspANI	GGCC	5 cut(s)	758, 1454, 1467, 2117, 2695
BspCNI	CTCAG	7 cut(s)	30, 137, 394, 1692, 2392, 2609, 2632
BspDI	ATCGAT	2 cut(s)	1356, 1604
BspFNI	CGCG	2 cut(s)	144, 1170
BspHI	TCATGA	1 cut(s)	2448
BspLI	GGNNCC	2 cut(s)	216, 2637
BspMI	ACCTGC	1 cut(s)	2084
BspPI	GGATC	3 cut(s)	1747, 2022, 2611
BspT104I	TTCGAA	1 cut(s)	57
BspT107I	GGYRCC	1 cut(s)	214
BspTNI	GGTCTC	3 cut(s)	208, 1477, 1892
BsrDI	GCAATG	2 cut(s)	1719, 2434
BsrGI	TGTACA	1 cut(s)	2286
BsrI	ACTGG	1 cut(s)	2130
BssECI	CCNNGG	5 cut(s)	231, 247, 762, 1487, 2118
BssMI	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
BssNAI	GTATAC	1 cut(s)	1952
BssT1I	CCWWGG	3 cut(s)	231, 247, 2118
Bst1107I	GTATAC	1 cut(s)	1952
Bst2UI	CCWGG	3 cut(s)	752, 1119, 1488
Bst4CI	ACNGT	7 cut(s)	842, 928, 1126, 1873, 1978, 2408, 2522
Bst6I	CTCTTC	2 cut(s)	876, 1897
BstAPI	GCANNNNNTGC	1 cut(s)	2327
BstAUI	TGTACA	1 cut(s)	2286
BstBI	TTCGAA	1 cut(s)	57
BstC8I	GCNNGC	5 cut(s)	905, 1101, 1494, 1498, 1701
BstDEI	CTNAG	8 cut(s)	38, 124, 381, 783, 1679, 2379, 2596, 2640
BstDSI	CCRYGG	1 cut(s)	247
BstEII	GGTNACC	1 cut(s)	1336
BstF5I	GGATG	1 cut(s)	2480
BstFNI	CGCG	2 cut(s)	144, 1170
BstH2I	RGCGCY	1 cut(s)	2425
BstHHI	GCGC	2 cut(s)	2086, 2424
BstKTI	GATC	7 cut(s)	1356, 1742, 1881, 2017, 2341, 2434, 2619
BstMAI	GTCTC	4 cut(s)	156, 208, 1477, 1892
BstMBI	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
BstMWI	GCNNNNNNNGC	7 cut(s)	386, 1709, 2090, 2199, 2327, 2393, 2421
BstNI	CCWGG	3 cut(s)	752, 1119, 1488
BstNSI	RCATGY	4 cut(s)	1496, 1703, 1939, 2234
BstPI	GGTNACC	1 cut(s)	1336
BstSCI	CCNGG	5 cut(s)	750, 761, 762, 1117, 1486
BstSFI	CTRYAG	2 cut(s)	604, 1752
BstSLI	GKGCMC	2 cut(s)	1813, 2398
BstUI	CGCG	2 cut(s)	144, 1170
BstV1I	GCAGC	5 cut(s)	114, 122, 887, 1512, 2255
BstV2I	GAAGAC	3 cut(s)	179, 1640, 2136
BstX2I	RGATCY	1 cut(s)	2616
BstYI	RGATCY	1 cut(s)	2616
BstZ17I	GTATAC	1 cut(s)	1952
Bsu15I	ATCGAT	2 cut(s)	1356, 1604
Bsu36I	CCTNAGG	1 cut(s)	2640
BsuRI	GGCC	5 cut(s)	758, 1454, 1467, 2117, 2695
BsuTUI	ATCGAT	2 cut(s)	1356, 1604
BtgI	CCRYGG	1 cut(s)	247
BtsCI	GGATG	1 cut(s)	2480
BtsIMutI	CAGTG	2 cut(s)	1139, 2527
BveI	ACCTGC	1 cut(s)	2084
Cac8I	GCNNGC	5 cut(s)	905, 1101, 1494, 1498, 1701
CciI	TCATGA	1 cut(s)	2448
CfoI	GCGC	2 cut(s)	2086, 2424
Cfr13I	GGNCC	1 cut(s)	1233
Cfr9I	CCCGGG	1 cut(s)	762
ClaI	ATCGAT	2 cut(s)	1356, 1604
CseI	GACGC	2 cut(s)	79, 1176
Csp6I	GTAC	6 cut(s)	711, 817, 918, 929, 2287, 2357
CviQI	GTAC	6 cut(s)	711, 817, 918, 929, 2287, 2357
DdeI	CTNAG	8 cut(s)	38, 124, 381, 783, 1679, 2379, 2596, 2640
DpnI	GATC	7 cut(s)	1355, 1741, 1880, 2016, 2340, 2433, 2618
DpnII	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
DriI	GACNNNNNGTC	1 cut(s)	955
Eam1104I	CTCTTC	2 cut(s)	876, 1897
Eam1105I	GACNNNNNGTC	1 cut(s)	955
EarI	CTCTTC	2 cut(s)	876, 1897
Ecl136II	GAGCTC	3 cut(s)	44, 1246, 1416
Eco130I	CCWWGG	3 cut(s)	231, 247, 2118
Eco147I	AGGCCT	1 cut(s)	758
Eco24I	GRGCYC	3 cut(s)	46, 1248, 1418
Eco31I	GGTCTC	3 cut(s)	208, 1477, 1892
Eco32I	GATATC	1 cut(s)	2581
Eco47I	GGWCC	1 cut(s)	1233
Eco53kI	GAGCTC	3 cut(s)	44, 1246, 1416
Eco57I	CTGAAG	1 cut(s)	1751
Eco81I	CCTNAGG	1 cut(s)	2640
Eco88I	CYCGRG	3 cut(s)	266, 683, 762
Eco91I	GGTNACC	1 cut(s)	1336
EcoICRI	GAGCTC	3 cut(s)	44, 1246, 1416
EcoO65I	GGTNACC	1 cut(s)	1336
EcoRII	CCWGG	3 cut(s)	750, 1117, 1486
EcoRV	GATATC	1 cut(s)	2581
EcoT14I	CCWWGG	3 cut(s)	231, 247, 2118
EcoT38I	GRGCYC	3 cut(s)	46, 1248, 1418
ErhI	CCWWGG	3 cut(s)	231, 247, 2118
Esp3I	CGTCTC	1 cut(s)	156
FalI	AAGNNNNNCTT	4 cut(s)	1365, 1397, 1913, 1945
FaqI	GGGAC	1 cut(s)	2526
FauI	CCCGC	1 cut(s)	149
FbaI	TGATCA	2 cut(s)	1878, 2431
FblI	GTMKAC	3 cut(s)	959, 1860, 1951
FokI	GGATG	1 cut(s)	2467
FriOI	GRGCYC	3 cut(s)	46, 1248, 1418
FspBI	CTAG	4 cut(s)	525, 1842, 2199, 2621
GlaI	GCGC	2 cut(s)	2085, 2423
GsuI	CTGGAG	1 cut(s)	734
HaeII	RGCGCY	1 cut(s)	2425
HaeIII	GGCC	5 cut(s)	758, 1454, 1467, 2117, 2695
HapII	CCGG	2 cut(s)	99, 763
HgaI	GACGC	2 cut(s)	79, 1176
HhaI	GCGC	2 cut(s)	2086, 2424
Hin6I	GCGC	2 cut(s)	2084, 2422
HinP1I	GCGC	2 cut(s)	2084, 2422
HincII	GTYRAC	3 cut(s)	130, 960, 1379
HindII	GTYRAC	3 cut(s)	130, 960, 1379
HindIII	AAGCTT	3 cut(s)	854, 864, 1922
HinfI	GANTC	7 cut(s)	157, 330, 742, 787, 2073, 2464, 2506
HpaII	CCGG	2 cut(s)	99, 763
HphI	GGTGA	4 cut(s)	823, 1808, 2515, 2555
Hpy166II	GTNNAC	9 cut(s)	130, 924, 960, 1010, 1379, 1811, 1861, 1952, 2396
Hpy188III	TCNNGA	5 cut(s)	1670, 1720, 1876, 2243, 2449
Hpy8I	GTNNAC	9 cut(s)	130, 924, 960, 1010, 1379, 1811, 1861, 1952, 2396
Hpy99I	CGWCG	2 cut(s)	73, 86
HpyCH4III	ACNGT	7 cut(s)	842, 928, 1126, 1873, 1978, 2408, 2522
HpyCH4IV	ACGT	5 cut(s)	30, 815, 920, 1400, 1965
HpyF10VI	GCNNNNNNNGC	7 cut(s)	386, 1709, 2090, 2199, 2327, 2393, 2421
HpyF3I	CTNAG	8 cut(s)	38, 124, 381, 783, 1679, 2379, 2596, 2640
HpySE526I	ACGT	5 cut(s)	30, 815, 920, 1400, 1965
HspAI	GCGC	2 cut(s)	2084, 2422
Ksp22I	TGATCA	2 cut(s)	1878, 2431
Kzo9I	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
LmnI	GCTCC	3 cut(s)	49, 180, 249
Lsp1109I	GCAGC	5 cut(s)	114, 122, 887, 1512, 2255
LweI	GCATC	5 cut(s)	1086, 1596, 1610, 1716, 2116
MaeI	CTAG	4 cut(s)	525, 1842, 2199, 2621
MaeII	ACGT	5 cut(s)	30, 815, 920, 1400, 1965
MalI	GATC	7 cut(s)	1355, 1741, 1880, 2016, 2340, 2433, 2618
MboI	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
MfeI	CAATTG	2 cut(s)	169, 2188
MflI	RGATCY	1 cut(s)	2616
MhlI	GDGCHC	5 cut(s)	46, 1248, 1418, 1813, 2398
MluI	ACGCGT	1 cut(s)	1168
MlyI	GAGTC	2 cut(s)	751, 796
MmeI	TCCRAC	6 cut(s)	92, 454, 510, 675, 1803, 1992
MroXI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
MslI	CAYNNNNRTG	2 cut(s)	795, 1874
MspI	CCGG	2 cut(s)	99, 763
MspR9I	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
MunI	CAATTG	2 cut(s)	169, 2188
Mva1269I	GAATGC	1 cut(s)	298
MvaI	CCWGG	3 cut(s)	752, 1119, 1488
MvnI	CGCG	2 cut(s)	144, 1170
MwoI	GCNNNNNNNGC	7 cut(s)	386, 1709, 2090, 2199, 2327, 2393, 2421
NciI	CCSGG	2 cut(s)	763, 764
NcoI	CCATGG	1 cut(s)	247
NdeII	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
NlaIV	GGNNCC	2 cut(s)	216, 2637
NmeAIII	GCCGAG	2 cut(s)	131, 933
NmuCI	GTSAC	3 cut(s)	811, 947, 1756
NspI	RCATGY	4 cut(s)	1496, 1703, 1939, 2234
NspV	TTCGAA	1 cut(s)	57
OliI	CACNNNNGTG	1 cut(s)	1874
PaeI	GCATGC	2 cut(s)	1496, 1703
PagI	TCATGA	1 cut(s)	2448
PceI	AGGCCT	1 cut(s)	758
PctI	GAATGC	1 cut(s)	298
PdmI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
PfeI	GAWTC	5 cut(s)	157, 330, 2073, 2464, 2506
Pfl23II	CGTACG	1 cut(s)	917
PfoI	TCCNGGA	1 cut(s)	750
PleI	GAGTC	2 cut(s)	750, 795
PpsI	GAGTC	2 cut(s)	750, 795
PshBI	ATTAAT	1 cut(s)	618
PsiI	TTATAA	1 cut(s)	1272
Psp124BI	GAGCTC	3 cut(s)	46, 1248, 1418
Psp1406I	AACGTT	1 cut(s)	1400
Psp6I	CCWGG	3 cut(s)	750, 1117, 1486
PspEI	GGTNACC	1 cut(s)	1336
PspGI	CCWGG	3 cut(s)	750, 1117, 1486
PspLI	CGTACG	1 cut(s)	917
PspN4I	GGNNCC	2 cut(s)	216, 2637
PspPI	GGNCC	1 cut(s)	1233
PsuI	RGATCY	1 cut(s)	2616
RsaI	GTAC	6 cut(s)	712, 818, 919, 930, 2288, 2358
RsaNI	GTAC	6 cut(s)	711, 817, 918, 929, 2287, 2357
RseI	CAYNNNNRTG	2 cut(s)	795, 1874
SacI	GAGCTC	3 cut(s)	46, 1248, 1418
SalI	GTCGAC	1 cut(s)	958
Sau3AI	GATC	7 cut(s)	1353, 1739, 1878, 2014, 2338, 2431, 2616
Sau96I	GGNCC	1 cut(s)	1233
ScaI	AGTACT	1 cut(s)	930
SchI	GAGTC	2 cut(s)	751, 796
ScrFI	CCNGG	5 cut(s)	752, 763, 764, 1119, 1488
SduI	GDGCHC	5 cut(s)	46, 1248, 1418, 1813, 2398
SfaNI	GCATC	5 cut(s)	1086, 1596, 1610, 1716, 2116
SfcI	CTRYAG	2 cut(s)	604, 1752
SfuI	TTCGAA	1 cut(s)	57
SinI	GGWCC	1 cut(s)	1233
SmaI	CCCGGG	1 cut(s)	764
SmiMI	CAYNNNNRTG	2 cut(s)	795, 1874
SmlI	CTYRAG	1 cut(s)	191
SmoI	CTYRAG	1 cut(s)	191
SphI	GCATGC	2 cut(s)	1496, 1703
SseBI	AGGCCT	1 cut(s)	758
SsiI	CCGC	6 cut(s)	142, 228, 252, 1703, 1846, 2367
SspI	AATATT	2 cut(s)	1348, 1770
SspMI	CTAG	4 cut(s)	525, 1842, 2199, 2621
SstI	GAGCTC	3 cut(s)	46, 1248, 1418
StuI	AGGCCT	1 cut(s)	758
StyD4I	CCNGG	5 cut(s)	750, 761, 762, 1117, 1486
StyI	CCWWGG	3 cut(s)	231, 247, 2118
TaaI	ACNGT	7 cut(s)	842, 928, 1126, 1873, 1978, 2408, 2522
TaiI	ACGT	5 cut(s)	33, 818, 923, 1403, 1968
TatI	WGTACW	4 cut(s)	710, 928, 2286, 2356
TauI	GCSGC	5 cut(s)	230, 255, 1706, 1848, 2370
TfiI	GAWTC	5 cut(s)	157, 330, 2073, 2464, 2506
TscAI	CASTG	2 cut(s)	1146, 2527
TseFI	GTSAC	3 cut(s)	811, 947, 1756
TseI	GCWGC	5 cut(s)	102, 110, 900, 1500, 2268
Tsp45I	GTSAC	3 cut(s)	811, 947, 1756
TspDTI	ATGAA	6 cut(s)	821, 876, 1010, 1557, 2124, 2437
TspGWI	ACGGA	3 cut(s)	70, 1914, 2473
TspMI	CCCGGG	1 cut(s)	762
TspRI	CASTG	2 cut(s)	1146, 2527
VneI	GTGCAC	2 cut(s)	1809, 2394
VpaK11BI	GGWCC	1 cut(s)	1233
VspI	ATTAAT	1 cut(s)	618
XapI	RAATTY	2 cut(s)	343, 1530
XceI	RCATGY	4 cut(s)	1496, 1703, 1939, 2234
XmaI	CCCGGG	1 cut(s)	762
XmiI	GTMKAC	3 cut(s)	959, 1860, 1951
XmnI	GAANNNNTTC	3 cut(s)	867, 1923, 2077
XspI	CTAG	4 cut(s)	525, 1842, 2199, 2621
ZrmI	AGTACT	1 cut(s)	930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.