pycom10g14710
TCP Family

Chaperonin CPN60-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029275
Physical Location & Seq
Reverse (-)
18110974 .. 18114078
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1428 bp
ATGCTTCTAGACCCTTCTCTCCTGCTCAATTTATACTCTCCTGCTCTCCCCCTCTCTCTCACTCTCACCTCGCTTTTTCCTGATTGCCCATCTTCTTTCTCAATCTACTTTCTCTCTCTAAGGTGCTCTGCAACTTCGTCAATCATGTATCGCTTCGCCACTAACCTTGCCTCCAAAGCCAGGGTGGCTAGGAACAGTTCTCAACAGATTGGTAGTAAATTGATTTCGAGAAGGAACTATGCGGCGAAAGAAATCAGATTCGGTGTTGAAGCCCGGGCCGGGATGCTTAGGGGTGTGGAAGAGCTCGCTGATGCTGTTAAAGTCACAATGGGGCCAAAAGGGCGCAATGTTGTTCTAGAACAAAGCTTTGGTGCCCCTAAAGTGACCAAGGACGGTGTCACTGTAGCAAAGAGTATTGAGTTCAAGGATAAAGTCAAGAACATTGGTGCTAGTCTTGTAAAGCAGGTTGCAAATGCTACAAACGATGCAGCAGGAGATGGTACCACTTGTGCTACAGTCCTTGCCCGCGCTATATTTACTGAAGGATGCAAGTCAGTGGCGGCTGGAATGAATGCTATGGACCTAAGACGAGGCATTTCAATGGCAGTTGATTCCGTTGTTGCAAACTTGAAGAGTAGGGCTCGGATGATCAGCACATCTGAAGAAATAGCTCAGGTTGGTACAATATCAGCAAACGGAGAGAGGGAAATTGGTGAGCTAATAGCTAAGGCTATGGAGAAAGTTGGCAAAGAGGGTGTTATAACCATAGCTGATGGAAAAACACTGTATAACGAGTTGGAGGTCGTTGAAGGAATGAAGCTGGATAGGGGCTACATATCCCCTTATTTTATCACCAATCCTAAGAACCAAAAATGTGAATTAGAAGACCCACTCATCCTGATCCATGAGAAGAAACTCTCAAATCTAAATTCTATAGTGAAAATATTAGAGTTGGCCTTGCAGAAACAAAGACCTCTTGTGATTGTTGCGGAAGATGTGGAAAGTGAAGCTCTAGCGACGCTCATTATAAACAAGCTTCGTGCTGGAATTAAGGTTTGTGCAATTAAAGCCCCTGGATTTGGAGAAAACAGGAAGGCAAATTTGCAGGACCTAGCCATTCTTACGGGAGCCCAGGTAATAACTGAAGAGCTTGGTCTGAACGTTGAAAAAGTGGGAGTAGAAACACTTGGAACATGCAAAAGGATTACGGTATCAAAAGATGACACCGTCATTCTTGATGGGGCTGGTGACAAGAAAGCCATTGAAGAAAGATGTGAACAGCTGAGATCATCAATTGAATTGAGCACTTCTGATTATGACAAAGAGAAGTTACAAGAGCGCCTTGCGAAGATTTCTGGTGGTGTTGCCGTCTTAAAGGTTGTGGTGGAGCTAGCGAAGCAGAAGTTGGTGAAAAGAAGGATAGAGTAA

Protein Analysis

476

Amino Acids

51.34

Weight (kDa)

9.04

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 102 - 308 1.2e-34 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029275)

Species Orthologous Gene IDs
pyrus_communis pycom05g16770 pycom10g14710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 761, 1028
Acc36I ACCTGC 1 cut(s) 454
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 371, 500
AccII CGCG 1 cut(s) 528
AciI CCGC 4 cut(s) 242, 526, 560, 989
AclI AACGTT 1 cut(s) 1161
AclWI GGATC 1 cut(s) 895
AcsI RAATTY 2 cut(s) 928, 1099
AcuI CTGAAG 3 cut(s) 561, 681, 1164
AfaI GTAC 2 cut(s) 502, 682
AfiI CCNNNNNNNGG 3 cut(s) 180, 279, 1079
AgsI TTSAA 8 cut(s) 269, 424, 600, 631, 809, 1166, 1265, 1298
AjnI CCWGG 3 cut(s) 179, 1072, 1131
AjuI GAANNNNNNNTTGG 4 cut(s) 351, 383, 1388, 1420
Alw21I GWGCWC 3 cut(s) 128, 306, 1307
AlwI GGATC 1 cut(s) 895
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 3 cut(s) 276, 332, 954
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 2 cut(s) 928, 1099
Asp718I GGTACC 1 cut(s) 500
AspLEI GCGC 3 cut(s) 345, 530, 1341
AspS9I GGNCC 4 cut(s) 276, 332, 580, 1108
AsuC2I CCSGG 3 cut(s) 274, 275, 280
AsuHPI GGTGA 5 cut(s) 58, 725, 844, 1259, 1420
AsuNHI GCTAGC 1 cut(s) 1390
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 2 cut(s) 580, 1108
BaeGI GKGCMC 1 cut(s) 376
BanI GGYRCC 2 cut(s) 371, 500
BanII GRGCYC 3 cut(s) 306, 643, 1132
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 3 cut(s) 128, 306, 1307
BbvI GCAGC 1 cut(s) 500
BccI CCATC 4 cut(s) 97, 491, 767, 1232
BceAI ACGGC 1 cut(s) 1352
BciT130I CCWGG 3 cut(s) 181, 1074, 1133
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 3 cut(s) 274, 275, 280
BfaI CTAG 7 cut(s) 8, 189, 356, 450, 1013, 1112, 1391
BfmI CTRYAG 3 cut(s) 402, 513, 933
BfoI RGCGCY 1 cut(s) 1342
BfuAI ACCTGC 1 cut(s) 454
BglI GCCNNNNNGGC 2 cut(s) 185, 340
BisI GCNGC 3 cut(s) 243, 489, 561
BlsI GCNGC 3 cut(s) 244, 490, 562
Bme1390I CCNGG 6 cut(s) 181, 274, 275, 280, 1074, 1133
Bme18I GGWCC 2 cut(s) 580, 1108
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 4 cut(s) 276, 332, 580, 1108
BmiI GGNNCC 4 cut(s) 333, 373, 502, 1129
BmrFI CCNGG 6 cut(s) 181, 274, 275, 280, 1074, 1133
BmsI GCATC 4 cut(s) 273, 301, 475, 536
BmtI GCTAGC 1 cut(s) 1394
BpiI GAAGAC 1 cut(s) 891
BplI GAGNNNNNCTC 2 cut(s) 625, 657
Bpu10I CCTNAGC 3 cut(s) 287, 672, 726
BpuMI CCSGG 3 cut(s) 274, 275, 280
BsaJI CCNNGG 5 cut(s) 180, 273, 387, 1072, 1131
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 1119, 1149
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 279, 1079
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 3 cut(s) 181, 1074, 1133
BseDI CCNNGG 5 cut(s) 180, 273, 387, 1072, 1131
BseGI GGATG 4 cut(s) 288, 551, 651, 894
BseLI CCNNNNNNNGG 3 cut(s) 180, 279, 1079
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 686, 1274
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 528
BshFI GGCC 3 cut(s) 278, 334, 956
BshNI GGYRCC 2 cut(s) 371, 500
BsiHKAI GWGCWC 3 cut(s) 128, 306, 1307
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 2 cut(s) 274, 279
BslI CCNNNNNNNGG 3 cut(s) 180, 279, 1079
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 3 cut(s) 278, 334, 956
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 6 cut(s) 128, 306, 376, 643, 1132, 1307
Bsp143I GATC 3 cut(s) 648, 900, 1286
BspACI CCGC 4 cut(s) 242, 526, 560, 989
BspANI GGCC 3 cut(s) 278, 334, 956
BspCNI CTCAG 2 cut(s) 685, 1275
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 4 cut(s) 333, 373, 502, 1129
BspMI ACCTGC 1 cut(s) 454
BspOI GCTAGC 1 cut(s) 1394
BspPI GGATC 1 cut(s) 895
BspQI GCTCTTC 2 cut(s) 294, 1140
BspT107I GGYRCC 2 cut(s) 371, 500
BsrDI GCAATG 1 cut(s) 352
BssECI CCNNGG 5 cut(s) 180, 273, 387, 1072, 1131
BssMI GATC 3 cut(s) 648, 900, 1286
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 3 cut(s) 181, 1074, 1133
Bst4CI ACNGT 7 cut(s) 197, 395, 403, 517, 786, 1210, 1228
Bst6I CTCTTC 3 cut(s) 294, 626, 1140
BstC8I GCNNGC 3 cut(s) 306, 526, 1392
BstDEI CTNAG 7 cut(s) 119, 287, 584, 672, 726, 861, 1283
BstF5I GGATG 4 cut(s) 288, 551, 651, 894
BstFNI CGCG 1 cut(s) 528
BstH2I RGCGCY 1 cut(s) 1342
BstHHI GCGC 3 cut(s) 345, 530, 1341
BstKTI GATC 3 cut(s) 651, 903, 1289
BstMBI GATC 3 cut(s) 648, 900, 1286
BstMWI GCNNNNNNNGC 5 cut(s) 176, 185, 340, 1067, 1396
BstNI CCWGG 3 cut(s) 181, 1074, 1133
BstNSI RCATGY 1 cut(s) 1197
BstSCI CCNGG 6 cut(s) 179, 272, 273, 278, 1072, 1131
BstSFI CTRYAG 3 cut(s) 402, 513, 933
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 1 cut(s) 891
BsuRI GGCC 3 cut(s) 278, 334, 956
BtsCI GGATG 4 cut(s) 288, 551, 651, 894
BtsIMutI CAGTG 3 cut(s) 399, 561, 782
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 3 cut(s) 306, 526, 1392
CfoI GCGC 3 cut(s) 345, 530, 1341
Cfr13I GGNCC 4 cut(s) 276, 332, 580, 1108
Cfr9I CCCGGG 1 cut(s) 273
CseI GACGC 1 cut(s) 1027
Csp6I GTAC 2 cut(s) 501, 681
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 3 cut(s) 145, 905, 1194
CviQI GTAC 2 cut(s) 501, 681
DdeI CTNAG 7 cut(s) 119, 287, 584, 672, 726, 861, 1283
DpnI GATC 3 cut(s) 650, 902, 1288
DpnII GATC 3 cut(s) 648, 900, 1286
Eam1104I CTCTTC 3 cut(s) 294, 626, 1140
EarI CTCTTC 3 cut(s) 294, 626, 1140
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 3 cut(s) 306, 643, 1132
Eco47I GGWCC 2 cut(s) 580, 1108
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 3 cut(s) 561, 681, 1164
Eco88I CYCGRG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRII CCWGG 3 cut(s) 179, 1072, 1131
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 3 cut(s) 306, 643, 1132
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 3 cut(s) 148, 908, 1197
FalI AAGNNNNNCTT 2 cut(s) 1326, 1358
FatI CATG 3 cut(s) 144, 904, 1193
FauI CCCGC 1 cut(s) 533
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 3 cut(s) 243, 489, 561
FokI GGATG 4 cut(s) 295, 558, 658, 881
FriOI GRGCYC 3 cut(s) 306, 643, 1132
Fsp4HI GCNGC 3 cut(s) 243, 489, 561
FspBI CTAG 7 cut(s) 8, 189, 356, 450, 1013, 1112, 1391
GlaI GCGC 3 cut(s) 344, 529, 1340
GluI GCNGC 3 cut(s) 243, 489, 561
HaeII RGCGCY 1 cut(s) 1342
HaeIII GGCC 3 cut(s) 278, 334, 956
HapII CCGG 2 cut(s) 274, 279
HgaI GACGC 1 cut(s) 1027
HhaI GCGC 3 cut(s) 345, 530, 1341
Hin1II CATG 3 cut(s) 148, 908, 1197
Hin6I GCGC 3 cut(s) 343, 528, 1339
HinP1I GCGC 3 cut(s) 343, 528, 1339
HindIII AAGCTT 2 cut(s) 364, 1034
HinfI GANTC 2 cut(s) 258, 611
HpaII CCGG 2 cut(s) 274, 279
HphI GGTGA 5 cut(s) 58, 725, 844, 1259, 1420
Hpy166II GTNNAC 1 cut(s) 1277
Hpy188I TCNGA 5 cut(s) 257, 645, 661, 1158, 1312
Hpy188III TCNNGA 7 cut(s) 8, 80, 228, 356, 436, 898, 1235
Hpy8I GTNNAC 1 cut(s) 1277
Hpy99I CGWCG 1 cut(s) 1021
HpyAV CCTTC 6 cut(s) 24, 225, 536, 803, 1087, 1410
HpyCH4III ACNGT 7 cut(s) 197, 395, 403, 517, 786, 1210, 1228
HpyCH4IV ACGT 1 cut(s) 1161
HpyCH4V TGCA 9 cut(s) 131, 470, 488, 549, 623, 961, 1061, 1105, 1197
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 185, 340, 1067, 1396
HpyF3I CTNAG 7 cut(s) 119, 287, 584, 672, 726, 861, 1283
HpySE526I ACGT 1 cut(s) 1161
Hsp92II CATG 3 cut(s) 148, 908, 1197
HspAI GCGC 3 cut(s) 343, 528, 1339
KpnI GGTACC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 3 cut(s) 648, 900, 1286
LguI GCTCTTC 2 cut(s) 294, 1140
LmnI GCTCC 2 cut(s) 1127, 1387
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 4 cut(s) 273, 301, 475, 536
MaeI CTAG 7 cut(s) 8, 189, 356, 450, 1013, 1112, 1391
MaeII ACGT 1 cut(s) 1161
MaeIII GTNAC 5 cut(s) 322, 382, 397, 1247, 1329
MalI GATC 3 cut(s) 650, 902, 1288
MboI GATC 3 cut(s) 648, 900, 1286
MfeI CAATTG 1 cut(s) 1293
MhlI GDGCHC 6 cut(s) 128, 306, 376, 643, 1132, 1307
MmeI TCCRAC 1 cut(s) 777
MnlI CCTC 8 cut(s) 62, 79, 181, 584, 696, 745, 793, 984
MseI TTAA 4 cut(s) 318, 1050, 1065, 1373
MslI CAYNNNNRTG 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 1282
MspI CCGG 2 cut(s) 274, 279
MspR9I CCNGG 6 cut(s) 181, 274, 275, 280, 1074, 1133
MunI CAATTG 1 cut(s) 1293
Mva1269I GAATGC 1 cut(s) 577
MvaI CCWGG 3 cut(s) 181, 1074, 1133
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 5 cut(s) 176, 185, 340, 1067, 1396
NciI CCSGG 3 cut(s) 274, 275, 280
NdeII GATC 3 cut(s) 648, 900, 1286
NheI GCTAGC 1 cut(s) 1390
NlaIII CATG 3 cut(s) 148, 908, 1197
NlaIV GGNNCC 4 cut(s) 333, 373, 502, 1129
NmuCI GTSAC 4 cut(s) 322, 382, 397, 1247
NspI RCATGY 1 cut(s) 1197
PciSI GCTCTTC 2 cut(s) 294, 1140
PctI GAATGC 1 cut(s) 577
PfeI GAWTC 2 cut(s) 258, 611
PflFI GACNNNGTC 2 cut(s) 395, 1226
PkrI GCNGC 3 cut(s) 244, 490, 562
PpuMI RGGWCCY 1 cut(s) 1108
PsiI TTATAA 2 cut(s) 761, 1028
Psp124BI GAGCTC 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 1161
Psp5II RGGWCCY 1 cut(s) 1108
Psp6I CCWGG 3 cut(s) 179, 1072, 1131
PspGI CCWGG 3 cut(s) 179, 1072, 1131
PspN4I GGNNCC 4 cut(s) 333, 373, 502, 1129
PspPI GGNCC 4 cut(s) 276, 332, 580, 1108
PspPPI RGGWCCY 1 cut(s) 1108
PsyI GACNNNGTC 2 cut(s) 395, 1226
PvuII CAGCTG 1 cut(s) 1282
RsaI GTAC 2 cut(s) 502, 682
RsaNI GTAC 2 cut(s) 501, 681
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 2 cut(s) 294, 1140
SaqAI TTAA 4 cut(s) 318, 1050, 1065, 1373
SatI GCNGC 3 cut(s) 243, 489, 561
Sau3AI GATC 3 cut(s) 648, 900, 1286
Sau96I GGNCC 4 cut(s) 276, 332, 580, 1108
ScrFI CCNGG 6 cut(s) 181, 274, 275, 280, 1074, 1133
SduI GDGCHC 6 cut(s) 128, 306, 376, 643, 1132, 1307
SfaNI GCATC 4 cut(s) 273, 301, 475, 536
SfcI CTRYAG 3 cut(s) 402, 513, 933
SinI GGWCC 2 cut(s) 580, 1108
SmaI CCCGGG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 599
SrfI GCCCGGGC 1 cut(s) 275
SsiI CCGC 4 cut(s) 242, 526, 560, 989
SspI AATATT 1 cut(s) 945
SspMI CTAG 7 cut(s) 8, 189, 356, 450, 1013, 1112, 1391
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 6 cut(s) 179, 272, 273, 278, 1072, 1131
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 7 cut(s) 197, 395, 403, 517, 786, 1210, 1228
TaiI ACGT 1 cut(s) 1164
TaqI TCGA 1 cut(s) 227
TauI GCSGC 2 cut(s) 245, 563
TfiI GAWTC 2 cut(s) 258, 611
Tru1I TTAA 4 cut(s) 318, 1050, 1065, 1373
Tru9I TTAA 4 cut(s) 318, 1050, 1065, 1373
TscAI CASTG 3 cut(s) 406, 561, 789
TseFI GTSAC 4 cut(s) 322, 382, 397, 1247
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 4 cut(s) 322, 382, 397, 1247
TspDTI ATGAA 2 cut(s) 584, 830
TspGWI ACGGA 2 cut(s) 604, 711
TspMI CCCGGG 1 cut(s) 273
TspRI CASTG 3 cut(s) 406, 561, 789
Tth111I GACNNNGTC 2 cut(s) 395, 1226
VpaK11BI GGWCC 2 cut(s) 580, 1108
XapI RAATTY 2 cut(s) 928, 1099
XbaI TCTAGA 2 cut(s) 7, 355
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 273
XspI CTAG 7 cut(s) 8, 189, 356, 450, 1013, 1112, 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.