pycom10g17310

Peptidase, trypsin-like serine and cysteine proteases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024069
Physical Location & Seq
Forward (+)
20514488 .. 20516027
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGTTTCATCCCCTGCCTCCTTCGCTTGGACCTGGGGTATATCTCGGTGCAGTGGAGAGAGCTACCTCATTTATCACAGATGAGCTTTGGTATGGCATCTTTGCCGGAATTAACCCAGAGACGTTTGTGAGAGCAGATGGGGCATTCATTCCTTTTGCTGATGATTTTGACATGTGTACTGTTATTACATCCATGAAAGGAGTCGGAGAGATTGGTGACGTTAAGATTATTGACTTGCAGTCTCCGATCAGTACCCTTATTGGAAAGCAGGTGATGAAGGTTGGACGAAGTTCTGGTTTGACTGCTGGAACAGTGTTGGCCTATGCTCTTGAGTACAATGACGAGAAAGGCATATGCTTCTTAACTGATTTTCTTGTTGTTGGTGAGAACCAACAAACATTTGACCTTGAAGGAGACAGCGGGAGCCTCATAGTACTCAAAGGTGTGAATGGTGAGAAGCCACGTCCAATTGGGATCATATGGGGCGGTACTGCTAACCGAGGACGACTTAAATTAAAAATTGGCCAACCTCCTGAGAATTGGACAAGCGGGATCGATCTTGGACGTCTTCTGAAGCTCCTTGAACTTGACATTATTACAACTGATGAAGGGCTTAGAGTGGCTGTACAAGAACAAAGGACTGCTTCAGCAACGGCAATTGGATCGACTCTAGGCGACTCTTCCCCTCCATATGCGATGCTTCGAAAGGAAACCTCTGAGGAGAAGTTTGAGTCACTGGGTCTTCAAATTCAGCATCTTCCTTTAGAAGTGGAACCCAGCAGCCCACCTAGGAATCCGTCCTTAGTGGAAACAGACTTTCATCTGGAAGATGGGATCAAGACAGTCCCCAGTGTCGAACATCAGTTTATACCAAGCTTCATCAGAGGCTCCCCACTACATAAAAAGTACCAGATTGATAGAGCAGCTTCTGAGAATCTTTCGTCGCTAAGGAATGGCTGCGACGAAGATATTTGTTTTTCACTGCAGTTGGGCGACAATGAGGCCAAGAGAAGGCGTTCAGATGCTTCAACCAGTGCTGAAGAACGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

38.15

Weight (kDa)

4.92

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nal1_C PF25819 1 - 203 8.1e-116 Nal1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024069)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15790
pyrus_communis pycom10g17310
rosa_multiflora Rmu_sc0006854.1_g000014

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
AatII GACGTC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 259
AciI CCGC 3 cut(s) 420, 486, 549
AclWI GGATC 4 cut(s) 482, 560, 670, 842
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 2 cut(s) 593, 630
AcyI GRCGYC 1 cut(s) 565
AfaI GTAC 7 cut(s) 178, 253, 335, 435, 490, 627, 908
AfiI CCNNNNNNNGG 2 cut(s) 26, 1011
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 4 cut(s) 410, 584, 746, 1029
AhdI GACNNNNNGTC 1 cut(s) 238
AjiI CACGTC 1 cut(s) 464
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 5 cut(s) 62, 85, 577, 876, 926
AluI AGCT 5 cut(s) 62, 85, 577, 876, 926
Alw26I GTCTC 3 cut(s) 113, 246, 408
AlwI GGATC 4 cut(s) 482, 560, 670, 842
AlwNI CAGNNNCTG 1 cut(s) 929
AoxI GGCC 3 cut(s) 318, 523, 1002
ApeKI GCWGC 3 cut(s) 780, 923, 957
ApoI RAATTY 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 1015
AspA2I CCTAGG 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 29
AsuHPI GGTGA 4 cut(s) 227, 283, 395, 464
AsuII TTCGAA 1 cut(s) 703
AvaII GGWCC 1 cut(s) 29
AvrII CCTAGG 1 cut(s) 788
BalI TGGCCA 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 560, 734
BbvI GCAGC 3 cut(s) 792, 935, 944
BccI CCATC 2 cut(s) 131, 824
BceAI ACGGC 1 cut(s) 669
BcgI CGANNNNNNTGC 2 cut(s) 645, 679
BciT130I CCWGG 1 cut(s) 33
BcoDI GTCTC 3 cut(s) 113, 246, 408
BfaI CTAG 2 cut(s) 671, 789
BfmI CTRYAG 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 3 cut(s) 781, 924, 958
BlnI CCTAGG 1 cut(s) 788
BlsI GCNGC 3 cut(s) 782, 925, 959
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 1 cut(s) 29
BmeRI GACNNNNNGTC 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 3 cut(s) 425, 774, 889
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 2 cut(s) 746, 843
BmsI GCATC 4 cut(s) 105, 687, 763, 1012
BmuI ACTGGG 2 cut(s) 746, 843
BpiI GAAGAC 2 cut(s) 560, 734
Bpu10I CCTNAGC 1 cut(s) 947
Bpu14I TTCGAA 1 cut(s) 703
BpuEI CTTGAG 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 565
BsaJI CCNNGG 3 cut(s) 32, 499, 788
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 1011
Bse1I ACTGG 3 cut(s) 741, 849, 1032
BseBI CCWGG 1 cut(s) 33
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 3 cut(s) 32, 499, 788
BseGI GGATG 2 cut(s) 7, 188
BseLI CCNNNNNNNGG 2 cut(s) 26, 1011
BseMII CTCAG 3 cut(s) 525, 708, 921
BseNI ACTGG 3 cut(s) 741, 849, 1032
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 3 cut(s) 792, 935, 944
BseYI CCCAGC 1 cut(s) 776
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 3 cut(s) 320, 525, 1004
BshVI ATCGAT 1 cut(s) 555
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 2 cut(s) 26, 1011
BsmAI GTCTC 3 cut(s) 113, 246, 408
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 830
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 3 cut(s) 320, 525, 1004
Bsp119I TTCGAA 1 cut(s) 703
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 6 cut(s) 246, 474, 552, 556, 662, 834
BspACI CCGC 3 cut(s) 420, 486, 549
BspANI GGCC 3 cut(s) 320, 525, 1004
BspCNI CTCAG 3 cut(s) 526, 709, 922
BspDI ATCGAT 1 cut(s) 555
BspLI GGNNCC 3 cut(s) 425, 774, 889
BspMAI CTGCAG 1 cut(s) 987
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 4 cut(s) 482, 560, 670, 842
BspT104I TTCGAA 1 cut(s) 703
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 3 cut(s) 741, 849, 1032
BssECI CCNNGG 3 cut(s) 32, 499, 788
BssMI GATC 6 cut(s) 246, 474, 552, 556, 662, 834
BssNI GRCGYC 1 cut(s) 565
BssT1I CCWWGG 1 cut(s) 788
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 181, 313, 844
Bst6I CTCTTC 1 cut(s) 685
BstACI GRCGYC 1 cut(s) 565
BstAUI TGTACA 1 cut(s) 625
BstBI TTCGAA 1 cut(s) 703
BstDEI CTNAG 6 cut(s) 534, 614, 717, 802, 930, 947
BstF5I GGATG 2 cut(s) 7, 188
BstKTI GATC 6 cut(s) 249, 477, 555, 559, 665, 837
BstMAI GTCTC 3 cut(s) 113, 246, 408
BstMBI GATC 6 cut(s) 246, 474, 552, 556, 662, 834
BstMWI GCNNNNNNNGC 2 cut(s) 22, 140
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 983
BstV1I GCAGC 3 cut(s) 792, 935, 944
BstV2I GAAGAC 2 cut(s) 560, 734
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 3 cut(s) 320, 525, 1004
BsuTUI ATCGAT 1 cut(s) 555
BtgZI GCGATG 1 cut(s) 710
BtrI CACGTC 1 cut(s) 464
BtsCI GGATG 2 cut(s) 7, 188
BtsI GCAGTG 2 cut(s) 57, 980
BtsIMutI CAGTG 6 cut(s) 57, 318, 734, 856, 980, 1039
BveI ACCTGC 1 cut(s) 259
CaiI CAGNNNCTG 1 cut(s) 929
Cfr13I GGNCC 1 cut(s) 29
ClaI ATCGAT 1 cut(s) 555
Csp6I GTAC 7 cut(s) 177, 252, 334, 434, 489, 626, 907
CviAII CATG 2 cut(s) 172, 193
CviQI GTAC 7 cut(s) 177, 252, 334, 434, 489, 626, 907
DdeI CTNAG 6 cut(s) 534, 614, 717, 802, 930, 947
DpnI GATC 6 cut(s) 248, 476, 554, 558, 664, 836
DpnII GATC 6 cut(s) 246, 474, 552, 556, 662, 834
DriI GACNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 685
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 685
Eco130I CCWWGG 1 cut(s) 788
Eco47I GGWCC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 593, 630
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 788
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 2 cut(s) 175, 196
FalI AAGNNNNNCTT 2 cut(s) 628, 660
FaqI GGGAC 1 cut(s) 830
FatI CATG 2 cut(s) 171, 192
FauI CCCGC 2 cut(s) 413, 542
FauNDI CATATG 3 cut(s) 353, 479, 691
Fnu4HI GCNGC 3 cut(s) 781, 924, 958
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 3 cut(s) 781, 924, 958
FspBI CTAG 2 cut(s) 671, 789
GluI GCNGC 3 cut(s) 781, 924, 958
GsaI CCCAGC 1 cut(s) 780
HaeIII GGCC 3 cut(s) 320, 525, 1004
HapII CCGG 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 2 cut(s) 175, 196
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 6 cut(s) 201, 667, 677, 731, 793, 934
HpaII CCGG 1 cut(s) 105
HphI GGTGA 4 cut(s) 227, 283, 395, 464
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 7 cut(s) 206, 246, 573, 718, 884, 931, 1021
Hpy188III TCNNGA 4 cut(s) 329, 533, 824, 838
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 2 cut(s) 946, 965
HpyAV CCTTC 5 cut(s) 30, 271, 404, 602, 1005
HpyCH4III ACNGT 3 cut(s) 181, 313, 844
HpyCH4IV ACGT 4 cut(s) 122, 219, 463, 565
HpyCH4V TGCA 3 cut(s) 50, 238, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 140
HpyF3I CTNAG 6 cut(s) 534, 614, 717, 802, 930, 947
HpySE526I ACGT 4 cut(s) 122, 219, 463, 565
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 2 cut(s) 175, 196
Kzo9I GATC 6 cut(s) 246, 474, 552, 556, 662, 834
LmnI GCTCC 3 cut(s) 423, 582, 893
Lsp1109I GCAGC 3 cut(s) 792, 935, 944
LweI GCATC 4 cut(s) 105, 687, 763, 1012
MaeI CTAG 2 cut(s) 671, 789
MaeII ACGT 4 cut(s) 122, 219, 463, 565
MaeIII GTNAC 2 cut(s) 215, 732
MalI GATC 6 cut(s) 248, 476, 554, 558, 664, 836
MboI GATC 6 cut(s) 246, 474, 552, 556, 662, 834
MboII GAAGA 7 cut(s) 560, 672, 734, 749, 839, 977, 1052
MfeI CAATTG 2 cut(s) 468, 657
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 7 cut(s) 108, 468, 512, 519, 538, 657, 747
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 4 cut(s) 210, 661, 671, 740
MmeI TCCRAC 2 cut(s) 184, 262
Mox20I TGGCCA 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 1015
MscI TGGCCA 1 cut(s) 525
MseI TTAA 5 cut(s) 111, 222, 362, 510, 515
Msp20I TGGCCA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 1 cut(s) 33
MunI CAATTG 2 cut(s) 468, 657
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 22, 140
NdeI CATATG 3 cut(s) 353, 479, 691
NdeII GATC 6 cut(s) 246, 474, 552, 556, 662, 834
NlaIII CATG 2 cut(s) 175, 196
NlaIV GGNNCC 3 cut(s) 425, 774, 889
NmuCI GTSAC 2 cut(s) 215, 732
NspI RCATGY 1 cut(s) 175
NspV TTCGAA 1 cut(s) 703
PaqCI CACCTGC 1 cut(s) 259
PciI ACATGT 1 cut(s) 171
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 1015
PfeI GAWTC 2 cut(s) 793, 934
PkrI GCNGC 3 cut(s) 782, 925, 959
PleI GAGTC 4 cut(s) 209, 661, 671, 739
PpsI GAGTC 4 cut(s) 209, 661, 671, 739
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 776
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 3 cut(s) 425, 774, 889
PspPI GGNCC 1 cut(s) 29
PstI CTGCAG 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 929
RsaI GTAC 7 cut(s) 178, 253, 335, 435, 490, 627, 908
RsaNI GTAC 7 cut(s) 177, 252, 334, 434, 489, 626, 907
SaqAI TTAA 5 cut(s) 111, 222, 362, 510, 515
SatI GCNGC 3 cut(s) 781, 924, 958
Sau3AI GATC 6 cut(s) 246, 474, 552, 556, 662, 834
Sau96I GGNCC 1 cut(s) 29
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 4 cut(s) 210, 661, 671, 740
ScrFI CCNGG 1 cut(s) 33
SfaNI GCATC 4 cut(s) 105, 687, 763, 1012
SfcI CTRYAG 1 cut(s) 983
SfuI TTCGAA 1 cut(s) 703
SinI GGWCC 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 7 cut(s) 108, 468, 512, 519, 538, 657, 747
SsiI CCGC 3 cut(s) 420, 486, 549
SspMI CTAG 2 cut(s) 671, 789
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 788
TaaI ACNGT 3 cut(s) 181, 313, 844
TaiI ACGT 4 cut(s) 125, 222, 466, 568
TaqI TCGA 4 cut(s) 555, 665, 703, 855
TasI AATT 7 cut(s) 108, 468, 512, 519, 538, 657, 747
TatI WGTACW 4 cut(s) 176, 333, 433, 625
TfiI GAWTC 2 cut(s) 793, 934
Tru1I TTAA 5 cut(s) 111, 222, 362, 510, 515
Tru9I TTAA 5 cut(s) 111, 222, 362, 510, 515
TscAI CASTG 6 cut(s) 57, 318, 741, 856, 987, 1039
TseFI GTSAC 2 cut(s) 215, 732
TseI GCWGC 3 cut(s) 780, 923, 957
Tsp45I GTSAC 2 cut(s) 215, 732
TspDTI ATGAA 6 cut(s) 136, 209, 290, 621, 809, 868
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 6 cut(s) 57, 318, 741, 856, 987, 1039
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 1 cut(s) 747
XceI RCATGY 1 cut(s) 175
XmaJI CCTAGG 1 cut(s) 788
XmnI GAANNNNTTC 1 cut(s) 1015
XspI CTAG 2 cut(s) 671, 789
ZraI GACGTC 1 cut(s) 566
ZrmI AGTACT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.