pycom10g22940

Belongs to the SecY SEC61-alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029246
Physical Location & Seq
Forward (+)
25222742 .. 25223792
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22940.1

Sequence Viewer

Length: 543 bp
ATGCGCGTTATTCTTGCATCAAGCCGTGGGACTGTGATGGAGCTTGGTATAACCCCCATTGTGACATCTGGGATGGTCGTGCAGCTCTTGGCCGGTTCAAAGATCATTGAAGTTGACAACAATGTTCGTGAGGATCGTGCGCTATTAAATGGGGCACAAAAGTTGTTGGCCATCCTCATCGCTGTTGGTCAGGCTGTTGCTTATGTGGTTTCAGGAATGTATGGTAGTGTTGCTCAGCTTGGTGTCGGGAATGCGATTCTTATCATTGTTCAGCTCTGCTTTGCTGCAATTATTGTTATTTGCCTAGATGAACTTCTTCAGAAGGGATATGGTTTGGGATCCGGGATCTCCCTTTTTATAGCTACCAACATCTGTGAAAGCATCATCTGGAAGTCCTTTAGCCCTACAACTATCAATAGCGGACGGGGAGCTGAATTTGAAGGTGCCATCATTGCACTGTTCCATCTCTTGATCGACCAGGACCGACAAAGTTCGTGCTCTTCGCGAAGCTTTCTACAGGCAAAACCTGCCAAATGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Plug_translocon SecY

Protein Analysis

181

Amino Acids

19.08

Weight (kDa)

7.6

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 11 - 144 4.7e-32 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029246)

Species Orthologous Gene IDs
pyrus_communis pycom03g19370 pycom10g22940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 443
AccII CGCG 2 cut(s) 6, 505
AciI CCGC 1 cut(s) 420
AclWI GGATC 4 cut(s) 141, 333, 346, 353
AcoI YGGCCR 2 cut(s) 90, 168
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 1 cut(s) 302
AgsI TTSAA 3 cut(s) 99, 110, 440
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 7 cut(s) 43, 85, 238, 274, 362, 431, 510
AluI AGCT 7 cut(s) 43, 85, 238, 274, 362, 431, 510
Alw21I GWGCWC 1 cut(s) 500
AlwI GGATC 4 cut(s) 141, 333, 346, 353
AoxI GGCC 2 cut(s) 90, 168
ApeKI GCWGC 2 cut(s) 82, 284
ApoI RAATTY 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 6, 142
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 1 cut(s) 343
AvaII GGWCC 1 cut(s) 481
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 170
BamHI GGATCC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 94, 271
BccI CCATC 5 cut(s) 31, 67, 179, 455, 471
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 479
BcnI CCSGG 1 cut(s) 343
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 535
BisI GCNGC 2 cut(s) 83, 285
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 2 cut(s) 84, 286
Bme1390I CCNGG 2 cut(s) 343, 479
Bme18I GGWCC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 2 cut(s) 340, 445
BmrFI CCNGG 2 cut(s) 343, 479
BmsI GCATC 2 cut(s) 26, 390
Bpu1102I GCTNAGC 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 25
Bse118I RCCGGY 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 450
Bse8I GATNNNNATC 1 cut(s) 260
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 25
BseGI GGATG 2 cut(s) 78, 171
BseJI GATNNNNATC 1 cut(s) 260
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 2 cut(s) 94, 271
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 2 cut(s) 6, 505
BshFI GGCC 2 cut(s) 92, 170
BshNI GGYRCC 1 cut(s) 443
BsiHKAI GWGCWC 1 cut(s) 500
BsiSI CCGG 2 cut(s) 93, 342
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 92, 170
Bsp1286I GDGCHC 2 cut(s) 157, 500
Bsp143I GATC 5 cut(s) 102, 133, 338, 345, 471
Bsp1720I GCTNAGC 1 cut(s) 234
Bsp68I TCGCGA 1 cut(s) 505
BspACI CCGC 1 cut(s) 420
BspANI GGCC 2 cut(s) 92, 170
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 2 cut(s) 6, 505
BspLI GGNNCC 2 cut(s) 340, 445
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 4 cut(s) 141, 333, 346, 353
BspQI GCTCTTC 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 5 cut(s) 102, 133, 338, 345, 471
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 2 cut(s) 34, 459
Bst6I CTCTTC 1 cut(s) 505
BstAPI GCANNNNNTGC 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 234
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 2 cut(s) 78, 171
BstFNI CGCG 2 cut(s) 6, 505
BstHHI GCGC 2 cut(s) 6, 142
BstKTI GATC 5 cut(s) 105, 136, 341, 348, 474
BstMBI GATC 5 cut(s) 102, 133, 338, 345, 471
BstMWI GCNNNNNNNGC 2 cut(s) 452, 527
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 341, 477
BstSFI CTRYAG 1 cut(s) 515
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 2 cut(s) 6, 505
BstV1I GCAGC 2 cut(s) 94, 271
BstX2I RGATCY 2 cut(s) 338, 345
BstYI RGATCY 2 cut(s) 338, 345
BsuRI GGCC 2 cut(s) 92, 170
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 2 cut(s) 78, 171
BtsIMutI CAGTG 1 cut(s) 455
BtuMI TCGCGA 1 cut(s) 505
BveI ACCTGC 1 cut(s) 535
CfoI GCGC 2 cut(s) 6, 142
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 481
DdeI CTNAG 1 cut(s) 234
DpnI GATC 5 cut(s) 104, 135, 340, 347, 473
DpnII GATC 5 cut(s) 102, 133, 338, 345, 471
EaeI YGGCCR 2 cut(s) 90, 168
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 477
FaiI YATR 5 cut(s) 50, 204, 222, 330, 359
FaqI GGGAC 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 83, 285
FokI GGATG 2 cut(s) 85, 158
Fsp4HI GCNGC 2 cut(s) 83, 285
FspBI CTAG 1 cut(s) 305
GlaI GCGC 2 cut(s) 5, 141
GluI GCNGC 2 cut(s) 83, 285
HaeIII GGCC 2 cut(s) 92, 170
HapII CCGG 2 cut(s) 93, 342
HhaI GCGC 2 cut(s) 6, 142
Hin6I GCGC 2 cut(s) 4, 140
HinP1I GCGC 2 cut(s) 4, 140
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 1 cut(s) 256
HpaII CCGG 2 cut(s) 93, 342
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 6 cut(s) 128, 213, 247, 388, 469, 504
Hpy8I GTNNAC 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 316, 434
HpyCH4III ACNGT 2 cut(s) 34, 459
HpyCH4V TGCA 4 cut(s) 17, 82, 287, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 527
HpyF3I CTNAG 1 cut(s) 234
HspAI GCGC 2 cut(s) 4, 140
Kzo9I GATC 5 cut(s) 102, 133, 338, 345, 471
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 2 cut(s) 40, 428
LpnPI CCDG 9 cut(s) 54, 106, 176, 198, 355, 373, 464, 491, 503
Lsp1109I GCAGC 2 cut(s) 94, 271
LweI GCATC 2 cut(s) 26, 390
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 2 cut(s) 61, 536
MalI GATC 5 cut(s) 104, 135, 340, 347, 473
MboI GATC 5 cut(s) 102, 133, 338, 345, 471
MboII GAAGA 2 cut(s) 308, 492
MflI RGATCY 2 cut(s) 338, 345
MhlI GDGCHC 2 cut(s) 157, 500
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 2 cut(s) 288, 434
MluNI TGGCCA 1 cut(s) 170
MnlI CCTC 2 cut(s) 124, 185
Mox20I TGGCCA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 170
MseI TTAA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 2 cut(s) 93, 342
MspR9I CCNGG 2 cut(s) 343, 479
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 2 cut(s) 6, 505
MwoI GCNNNNNNNGC 2 cut(s) 452, 527
NciI CCSGG 1 cut(s) 343
NdeII GATC 5 cut(s) 102, 133, 338, 345, 471
NlaIV GGNNCC 2 cut(s) 340, 445
NmuCI GTSAC 1 cut(s) 61
NruI TCGCGA 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 341
PkrI GCNGC 2 cut(s) 84, 286
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 340, 445
PspPI GGNCC 1 cut(s) 481
PsuI RGATCY 2 cut(s) 338, 345
RruI TCGCGA 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 83, 285
Sau3AI GATC 5 cut(s) 102, 133, 338, 345, 471
Sau96I GGNCC 1 cut(s) 481
ScrFI CCNGG 2 cut(s) 343, 479
SduI GDGCHC 2 cut(s) 157, 500
SetI ASST 9 cut(s) 45, 87, 240, 276, 364, 433, 445, 512, 529
SfaNI GCATC 2 cut(s) 26, 390
SfcI CTRYAG 1 cut(s) 515
SinI GGWCC 1 cut(s) 481
Sse9I AATT 2 cut(s) 288, 434
SsiI CCGC 1 cut(s) 420
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 2 cut(s) 341, 477
TaaI ACNGT 2 cut(s) 34, 459
TaqI TCGA 1 cut(s) 474
TaqII GACCGA 1 cut(s) 498
TasI AATT 2 cut(s) 288, 434
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 2 cut(s) 82, 284
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.