pycom10g24580

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024455
Physical Location & Seq
Forward (+)
26412037 .. 26413693
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGGATCGATCATGTCGGAAACTTGAACTTGTCGTGGATCCATATCGAATTCTTGAAAGGTTCTTGTGGAGAAAGAATGCAACTTTTGCAATTTCTAGACATTATAAACGCTTTGACGTGTTTACTGAAGCTGAGGCAAATAAAGCTGCTGGAAAATACGACAATGCCTCTATTGACTTTCAGGTTGAATTTTACAAAAAGGAAGGTTTGACCCCATATTCTGAGGAAAAGCTTCCCATTACAAGTGACGTTCCTGAAGGATGTGTTATAATAAGGGAGCATGTCCCTATCAGCAACCTTGCCGCTTGTCTTTGGTTCCATGAAGTTGATCGTTTTACTTCCAGGGATCAGATTAGTTTTTCCACAGTGAGAGACCACATTCGGGAAAAGACAAACTGGACTATCAATATGTTCTACGACTGTGAAAGGCGCAATTTTGTTGTTCAGAAATATCATAGAGATGTATTGGAACACATGGCTCCTCCTCTTCCTAAGGCTGTTCCTCCACCACCGCTATTACCACCACCACCGTCACCCCCAACTTTAAGTAACGAACCGCCAGTTAAACCATCAACCGAAACTTCACCCGAGAGCATTGTAAAAGCCCCAGGTAAGAGGCCTCCAAGGCGTGGGAGAGATAGAAGGTCGGGCTCTAGACGTCACCGCAAAATCGTTGCCGGTGCTAGAGACATTGATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

235

Amino Acids

27.06

Weight (kDa)

9.46

Isoelectric Point (pI)

68.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 7 - 152 2.7e-70 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024455)

Species Orthologous Gene IDs
malus_domestica MD05G1338900.v1.1
pyrus_communis pycom10g24580
rosa_multiflora Rmu_co8449883.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 105, 269
AatII GACGTC 1 cut(s) 661
AccB7I CCANNNNNTGG 1 cut(s) 629
AciI CCGC 4 cut(s) 303, 512, 557, 664
AclWI GGATC 4 cut(s) 12, 32, 45, 354
AcsI RAATTY 2 cut(s) 48, 188
AcuI CTGAAG 2 cut(s) 147, 276
AcyI GRCGYC 1 cut(s) 658
AfiI CCNNNNNNNGG 2 cut(s) 382, 629
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 4 cut(s) 26, 56, 188, 699
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 2 cut(s) 341, 607
AluBI AGCT 3 cut(s) 131, 146, 232
AluI AGCT 3 cut(s) 131, 146, 232
Alw26I GTCTC 2 cut(s) 366, 681
AlwI GGATC 4 cut(s) 12, 32, 45, 354
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 1 cut(s) 617
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 2 cut(s) 48, 188
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 432
AsuHPI GGTGA 3 cut(s) 525, 576, 653
AvaI CYCGRG 1 cut(s) 587
AxyI CCTNAGG 1 cut(s) 492
BamHI GGATCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 653
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 577
BciT130I CCWGG 2 cut(s) 343, 609
BcoDI GTCTC 2 cut(s) 366, 681
BfaI CTAG 3 cut(s) 96, 654, 684
BglI GCCNNNNNGGC 1 cut(s) 625
BisI GCNGC 2 cut(s) 147, 303
BlsI GCNGC 2 cut(s) 148, 304
Bme1390I CCNGG 2 cut(s) 343, 609
BmeT110I CYCGRG 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 118
BmiI GGNNCC 3 cut(s) 39, 317, 480
BmrFI CCNGG 2 cut(s) 343, 609
Bpu10I CCTNAGC 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 7
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 658
BsaI GGTCTC 1 cut(s) 366
BsaJI CCNNGG 3 cut(s) 342, 607, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 629
Bse118I RCCGGY 1 cut(s) 677
Bse1I ACTGG 2 cut(s) 401, 560
Bse21I CCTNAGG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 343, 609
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 342, 607, 623
BseGI GGATG 1 cut(s) 266
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 382, 629
BseMII CTCAG 2 cut(s) 123, 213
BseNI ACTGG 2 cut(s) 401, 560
BseRI GAGGAG 2 cut(s) 471, 474
BseXI GCAGC 1 cut(s) 133
BshFI GGCC 1 cut(s) 619
BshVI ATCGAT 1 cut(s) 7
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 1 cut(s) 678
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 382, 629
BsmAI GTCTC 2 cut(s) 366, 681
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 1 cut(s) 619
Bso31I GGTCTC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 5 cut(s) 4, 8, 37, 328, 346
BspACI CCGC 4 cut(s) 303, 512, 557, 664
BspANI GGCC 1 cut(s) 619
BspCNI CTCAG 2 cut(s) 124, 214
BspDI ATCGAT 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 39, 317, 480
BspPI GGATC 4 cut(s) 12, 32, 45, 354
BspTNI GGTCTC 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 677
BsrI ACTGG 2 cut(s) 401, 560
BssAI RCCGGY 1 cut(s) 677
BssECI CCNNGG 3 cut(s) 342, 607, 623
BssMI GATC 5 cut(s) 4, 8, 37, 328, 346
BssNI GRCGYC 1 cut(s) 658
BssT1I CCWWGG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 343, 609
Bst4CI ACNGT 3 cut(s) 367, 422, 531
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 658
BstAPI GCANNNNNTGC 1 cut(s) 86
BstDEI CTNAG 3 cut(s) 132, 222, 492
BstF5I GGATG 1 cut(s) 266
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 5 cut(s) 7, 11, 40, 331, 349
BstMAI GTCTC 2 cut(s) 366, 681
BstMBI GATC 5 cut(s) 4, 8, 37, 328, 346
BstMWI GCNNNNNNNGC 3 cut(s) 86, 143, 625
BstNI CCWGG 2 cut(s) 343, 609
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 341, 607
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
Bsu15I ATCGAT 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 1 cut(s) 619
BsuTUI ATCGAT 1 cut(s) 7
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 372
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 677
ClaI ATCGAT 1 cut(s) 7
CviAII CATG 4 cut(s) 12, 281, 320, 475
CviJI RGCY 8 cut(s) 131, 146, 232, 479, 497, 605, 619, 651
CviKI_1 RGCY 8 cut(s) 131, 146, 232, 479, 497, 605, 619, 651
DdeI CTNAG 3 cut(s) 132, 222, 492
DpnI GATC 5 cut(s) 6, 10, 39, 330, 348
DpnII GATC 5 cut(s) 4, 8, 37, 328, 346
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 623
Eco147I AGGCCT 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 653
Eco31I GGTCTC 1 cut(s) 366
Eco57I CTGAAG 2 cut(s) 147, 276
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 341, 607
EcoT14I CCWWGG 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 4 cut(s) 15, 284, 323, 478
FaqI GGGAC 1 cut(s) 269
FatI CATG 4 cut(s) 11, 280, 319, 474
Fnu4HI GCNGC 2 cut(s) 147, 303
FokI GGATG 1 cut(s) 273
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 2 cut(s) 147, 303
FspBI CTAG 3 cut(s) 96, 654, 684
GlaI GCGC 1 cut(s) 431
GluI GCNGC 2 cut(s) 147, 303
HaeIII GGCC 1 cut(s) 619
HapII CCGG 1 cut(s) 678
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 658
Hin1II CATG 4 cut(s) 15, 284, 323, 478
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 695
HpaII CCGG 1 cut(s) 678
HphI GGTGA 3 cut(s) 525, 576, 653
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 4 cut(s) 18, 223, 351, 447
Hpy188III TCNNGA 5 cut(s) 53, 96, 254, 383, 654
Hpy8I GTNNAC 1 cut(s) 123
HpyAV CCTTC 3 cut(s) 197, 251, 636
HpyCH4III ACNGT 3 cut(s) 367, 422, 531
HpyCH4IV ACGT 3 cut(s) 117, 249, 658
HpyCH4V TGCA 2 cut(s) 80, 89
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 143, 625
HpyF3I CTNAG 3 cut(s) 132, 222, 492
HpySE526I ACGT 3 cut(s) 117, 249, 658
Hsp92I GRCGYC 1 cut(s) 658
Hsp92II CATG 4 cut(s) 15, 284, 323, 478
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 5 cut(s) 4, 8, 37, 328, 346
LmnI GCTCC 2 cut(s) 277, 484
Lsp1109I GCAGC 1 cut(s) 133
MaeI CTAG 3 cut(s) 96, 654, 684
MaeII ACGT 3 cut(s) 117, 249, 658
MaeIII GTNAC 4 cut(s) 245, 531, 548, 659
MalI GATC 5 cut(s) 6, 10, 39, 330, 348
MboI GATC 5 cut(s) 4, 8, 37, 328, 346
MboII GAAGA 1 cut(s) 479
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 4 cut(s) 48, 90, 188, 433
MnlI CCTC 8 cut(s) 127, 178, 217, 492, 495, 513, 609, 630
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 3 cut(s) 545, 564, 703
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 1 cut(s) 678
MspR9I CCNGG 2 cut(s) 343, 609
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 2 cut(s) 343, 609
MwoI GCNNNNNNNGC 3 cut(s) 86, 143, 625
NdeII GATC 5 cut(s) 4, 8, 37, 328, 346
NlaIII CATG 4 cut(s) 15, 284, 323, 478
NlaIV GGNNCC 3 cut(s) 39, 317, 480
NmuCI GTSAC 3 cut(s) 245, 531, 659
NspI RCATGY 1 cut(s) 284
PceI AGGCCT 1 cut(s) 619
PctI GAATGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 695
PflMI CCANNNNNTGG 1 cut(s) 629
PkrI GCNGC 2 cut(s) 148, 304
PsiI TTATAA 2 cut(s) 105, 269
Psp6I CCWGG 2 cut(s) 341, 607
PspGI CCWGG 2 cut(s) 341, 607
PspN4I GGNNCC 3 cut(s) 39, 317, 480
PsuI RGATCY 1 cut(s) 37
RseI CAYNNNNRTG 1 cut(s) 459
SaqAI TTAA 3 cut(s) 545, 564, 703
SatI GCNGC 2 cut(s) 147, 303
Sau3AI GATC 5 cut(s) 4, 8, 37, 328, 346
ScrFI CCNGG 2 cut(s) 343, 609
SduI GDGCHC 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 4 cut(s) 48, 90, 188, 433
SseBI AGGCCT 1 cut(s) 619
SsiI CCGC 4 cut(s) 303, 512, 557, 664
SspMI CTAG 3 cut(s) 96, 654, 684
StuI AGGCCT 1 cut(s) 619
StyD4I CCNGG 2 cut(s) 341, 607
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 3 cut(s) 367, 422, 531
TaiI ACGT 3 cut(s) 120, 252, 661
TaqI TCGA 2 cut(s) 7, 46
TasI AATT 4 cut(s) 48, 90, 188, 433
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 3 cut(s) 545, 564, 703
Tru9I TTAA 3 cut(s) 545, 564, 703
TscAI CASTG 1 cut(s) 372
TseFI GTSAC 3 cut(s) 245, 531, 659
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 3 cut(s) 245, 531, 659
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 1 cut(s) 372
Van91I CCANNNNNTGG 1 cut(s) 629
XapI RAATTY 2 cut(s) 48, 188
XbaI TCTAGA 2 cut(s) 95, 653
XceI RCATGY 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 3 cut(s) 96, 654, 684
ZraI GACGTC 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.