pycom10g26220

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029388
Physical Location & Seq
Forward (+)
27625191 .. 27625867
677 bp
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UTR
Exon/CDS
Intron
pycom10g26220.1

Sequence Viewer

Length: 501 bp
ATGAGATCTAGGGTTGCTGATTTTGGCCTGTCGAGACAAGAACAGAATAAAACTCGTTCGTCAAATATGAGGGGGACGGTTGGATACGTCGATCCTGAGTACATGTTGACGAAGACGTATACGAAGATATGCGATGTTTATAGTTTTGGAGTGTTGCTCCTTGAACTCATTACATACAGGAATCCGCAGCTGGGTCTCATGGAATATGTGGAACTTGCTGCCATCGACACAGAGGATAGACTCCCGTGGCAAGTGATTGTGGATTCTCACCTCGACGGAAAGTTCAACATAGGACAGCTGGCGAATGTAGCTGATTTCGCTTACAAATGTGCGAGTAGTGTGTCCAGAAACCGGCCGTCAATGAGGGACATTGTACAGACCTTATCGTGGATTTTGAAGATGAGGCATCACACGAGGAACTACACGTACTGCTCAACTCCTGCAGAAACTTTCATTGAAATCGATCTGCTGGAGAACGAAGATGCTTTGATGGAACGCTGA

Protein Analysis

167

Amino Acids

19.22

Weight (kDa)

5.63

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 127 5.5e-14 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 4 - 128 1.5e-12 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029388)

Species Orthologous Gene IDs
pyrus_communis pycom10g26220
rosa_multiflora Rmu_sc0009128.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 119
AciI CCGC 1 cut(s) 185
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 353
AfaI GTAC 3 cut(s) 101, 375, 428
AfiI CCNNNNNNNGG 3 cut(s) 191, 351, 387
AflIII ACRYGT 2 cut(s) 102, 423
AgsI TTSAA 4 cut(s) 164, 286, 397, 458
AluBI AGCT 3 cut(s) 190, 298, 311
AluI AGCT 3 cut(s) 190, 298, 311
Alw26I GTCTC 2 cut(s) 28, 200
AlwI GGATC 1 cut(s) 86
AoxI GGCC 2 cut(s) 25, 353
ApeKI GCWGC 2 cut(s) 187, 218
AsuHPI GGTGA 1 cut(s) 260
BauI CACGAG 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 199, 205
BccI CCATC 2 cut(s) 230, 484
BceAI ACGGC 1 cut(s) 340
BciVI GTATCC 1 cut(s) 77
BcoDI GTCTC 2 cut(s) 28, 200
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 441
BfuI GTATCC 1 cut(s) 77
BglII AGATCT 1 cut(s) 5
BisI GCNGC 2 cut(s) 188, 219
BlsI GCNGC 2 cut(s) 189, 220
BmsI GCATC 2 cut(s) 415, 472
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 141, 173
BpmI CTGGAG 1 cut(s) 491
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 351, 387
Bse118I RCCGGY 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 245
BseLI CCNNNNNNNGG 3 cut(s) 191, 351, 387
BseMII CTCAG 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 199, 205
BseYI CCCAGC 1 cut(s) 190
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 2 cut(s) 27, 355
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 352
BslFI GGGAC 2 cut(s) 88, 380
BslI CCNNNNNNNGG 3 cut(s) 191, 351, 387
BsmAI GTCTC 2 cut(s) 28, 200
BsmFI GGGAC 2 cut(s) 88, 380
BsnI GGCC 2 cut(s) 27, 355
Bso31I GGTCTC 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 3 cut(s) 5, 91, 463
BspACI CCGC 1 cut(s) 185
BspANI GGCC 2 cut(s) 27, 355
BspCNI CTCAG 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 462
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 3 cut(s) 5, 91, 463
BssNAI GTATAC 1 cut(s) 120
BssSI CACGAG 1 cut(s) 412
Bst1107I GTATAC 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 245
BstKTI GATC 3 cut(s) 8, 94, 466
BstMAI GTCTC 2 cut(s) 28, 200
BstMBI GATC 3 cut(s) 5, 91, 463
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 308, 317
BstNSI RCATGY 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 199, 205
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BstZ17I GTATAC 1 cut(s) 120
BstZI CGGCCG 1 cut(s) 353
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 1 cut(s) 77
BsuRI GGCC 2 cut(s) 27, 355
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 351
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 3 cut(s) 100, 374, 427
CviAII CATG 2 cut(s) 103, 199
CviJI RGCY 5 cut(s) 27, 190, 298, 311, 355
CviKI_1 RGCY 5 cut(s) 27, 190, 298, 311, 355
CviQI GTAC 3 cut(s) 100, 374, 427
DdeI CTNAG 1 cut(s) 96
DpnI GATC 3 cut(s) 7, 93, 465
DpnII GATC 3 cut(s) 5, 91, 463
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
EclXI CGGCCG 1 cut(s) 353
Eco31I GGTCTC 1 cut(s) 200
Eco52I CGGCCG 1 cut(s) 353
FaeI CATG 2 cut(s) 106, 202
FaiI YATR 9 cut(s) 68, 104, 120, 130, 141, 175, 200, 207, 290
FaqI GGGAC 2 cut(s) 88, 380
FatI CATG 2 cut(s) 102, 198
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 188, 219
Fsp4HI GCNGC 2 cut(s) 188, 219
FspBI CTAG 1 cut(s) 9
GluI GCNGC 2 cut(s) 188, 219
GsaI CCCAGC 1 cut(s) 194
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 2 cut(s) 27, 355
HapII CCGG 1 cut(s) 352
Hin1II CATG 2 cut(s) 106, 202
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HinfI GANTC 3 cut(s) 181, 240, 263
HpaII CCGG 1 cut(s) 352
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 108, 120
Hpy188III TCNNGA 3 cut(s) 33, 95, 345
Hpy8I GTNNAC 2 cut(s) 108, 120
Hpy99I CGWCG 2 cut(s) 92, 278
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 3 cut(s) 87, 116, 425
HpyCH4V TGCA 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 317
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 3 cut(s) 87, 116, 425
Hsp92II CATG 2 cut(s) 106, 202
Kzo9I GATC 3 cut(s) 5, 91, 463
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 9 cut(s) 41, 108, 163, 176, 284, 358, 365, 453, 455
Lsp1109I GCAGC 2 cut(s) 199, 205
LweI GCATC 2 cut(s) 415, 472
MaeI CTAG 1 cut(s) 9
MaeII ACGT 3 cut(s) 87, 116, 425
MalI GATC 3 cut(s) 7, 93, 465
MboI GATC 3 cut(s) 5, 91, 463
MboII GAAGA 4 cut(s) 124, 136, 409, 491
MflI RGATCY 1 cut(s) 5
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 6 cut(s) 63, 226, 281, 357, 396, 408
MspA1I CMGCKG 2 cut(s) 190, 298
MspI CCGG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 308, 317
NdeII GATC 3 cut(s) 5, 91, 463
NlaIII CATG 2 cut(s) 106, 202
NspI RCATGY 1 cut(s) 106
PciI ACATGT 1 cut(s) 102
PfeI GAWTC 2 cut(s) 181, 263
PkrI GCNGC 2 cut(s) 189, 220
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 1 cut(s) 426
PscI ACATGT 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 190
PsrI GAACNNNNNNTAC 2 cut(s) 410, 442
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 2 cut(s) 190, 298
RsaI GTAC 3 cut(s) 101, 375, 428
RsaNI GTAC 3 cut(s) 100, 374, 427
SatI GCNGC 2 cut(s) 188, 219
Sau3AI GATC 3 cut(s) 5, 91, 463
SchI GAGTC 1 cut(s) 234
SetI ASST 8 cut(s) 90, 119, 192, 273, 300, 313, 383, 428
SfaNI GCATC 2 cut(s) 415, 472
SfcI CTRYAG 1 cut(s) 441
SsiI CCGC 1 cut(s) 185
SspMI CTAG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 3 cut(s) 90, 119, 428
TaqI TCGA 5 cut(s) 32, 90, 225, 273, 462
TatI WGTACW 2 cut(s) 99, 373
TfiI GAWTC 2 cut(s) 181, 263
TseI GCWGC 2 cut(s) 187, 218
TspDTI ATGAA 1 cut(s) 442
TspGWI ACGGA 1 cut(s) 291
XceI RCATGY 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 119
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.