pycom10g27570

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029390
Physical Location & Seq
Forward (+)
28494153 .. 28494782
630 bp
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UTR
Exon/CDS
Intron
pycom10g27570.1

Sequence Viewer

Length: 423 bp
ATGTCTCCACTCCGAGTCTCCCCATTTTCAAGCTTAACAAGTGCACCGAGAAAGAGGTTTCCTGCGGATATAAAGAGTTTTTCCCATGAACTGAATTCAAAAGGTGTACGACCTTTTCCATTTTGGAAGCCTCGAGGCTTGAACAACCTGGAAGAAATGTTGGTTGCCATTAGGGCAAAGTTTGACAAAGCAAAGGAAGAAGTGAACTCTGATCTGGCTATATTTGCAGCAGATTTAGTTGGAATTCTTGAAAAAGATGCAGATAATCATCCAGAGTTGCAGGAAACCCTTGAGGATTTGCTGGTTTTGGCACGAAGTTGTGCAATGACATCATCTCCCTTTGATTCAGCAACATATTCATTTGTCCTGATAACAGCTTCCTTGGCAGATATTAGTTTACAAAGAACGGCACCAAAGGCGTAA

Protein Analysis

141

Amino Acids

15.47

Weight (kDa)

6.11

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IREH1 PF26031 42 - 112 5.2e-24 IREH1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029390)

Species Orthologous Gene IDs
pyrus_communis pycom10g27570
rosa_multiflora Rmu_co8315679.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 409
AciI CCGC 1 cut(s) 65
AcsI RAATTY 2 cut(s) 94, 243
AfaI GTAC 1 cut(s) 108
AgsI TTSAA 4 cut(s) 30, 99, 142, 251
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 2 cut(s) 33, 377
AluI AGCT 2 cut(s) 33, 377
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 9, 22
Alw44I GTGCAC 1 cut(s) 42
Ama87I CYCGRG 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 2 cut(s) 94, 243
AvaI CYCGRG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 9, 22
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 149
BmeT110I CYCGRG 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 267
BseMI GCAATG 1 cut(s) 330
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 239
BshNI GGYRCC 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 132
BsmAI GTCTC 2 cut(s) 9, 22
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 1 cut(s) 211
BspACI CCGC 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 1 cut(s) 211
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 2 cut(s) 9, 22
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 4 cut(s) 173, 224, 383, 416
BstNI CCWGG 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 239
BtsCI GGATG 1 cut(s) 268
Csp6I GTAC 1 cut(s) 107
CviAII CATG 1 cut(s) 86
CviJI RGCY 5 cut(s) 33, 130, 138, 218, 377
CviKI_1 RGCY 5 cut(s) 33, 130, 138, 218, 377
CviQI GTAC 1 cut(s) 107
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 132
EcoRI GAATTC 2 cut(s) 94, 243
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 71, 87, 221, 355
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
Hin1II CATG 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 2 cut(s) 15, 344
Hpy166II GTNNAC 4 cut(s) 44, 107, 205, 398
Hpy188I TCNGA 2 cut(s) 14, 211
Hpy188III TCNNGA 3 cut(s) 248, 272, 367
Hpy8I GTNNAC 4 cut(s) 44, 107, 205, 398
HpyCH4V TGCA 5 cut(s) 44, 227, 260, 280, 323
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 224, 383, 416
Hsp92II CATG 1 cut(s) 89
Kzo9I GATC 1 cut(s) 211
LpnPI CCDG 8 cut(s) 75, 134, 161, 200, 266, 285, 287, 380
Lsp1109I GCAGC 1 cut(s) 239
LweI GCATC 1 cut(s) 247
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 2 cut(s) 164, 209
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 2 cut(s) 94, 243
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 4 cut(s) 48, 128, 141, 286
MseI TTAA 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 149
MvaI CCWGG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 173, 224, 383, 416
NdeII GATC 1 cut(s) 211
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 132
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 411
PspXI VCTCGAGB 1 cut(s) 132
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 1 cut(s) 211
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 1 cut(s) 46
SetI ASST 6 cut(s) 35, 59, 106, 115, 150, 379
SfaNI GCATC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmlI CTYRAG 2 cut(s) 132, 290
SmoI CTYRAG 2 cut(s) 132, 290
Sse9I AATT 2 cut(s) 94, 243
SsiI CCGC 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 381
TaqI TCGA 1 cut(s) 133
TasI AATT 2 cut(s) 94, 243
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 102, 348
VneI GTGCAC 1 cut(s) 42
XapI RAATTY 2 cut(s) 94, 243
XhoI CTCGAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.