pycom111g01450

carboxylesterase 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0011063
Physical Location & Seq
Forward (+)
929973 .. 932179
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01450.1

Sequence Viewer

Length: 1020 bp
ATGCCAAGCGTAGCTGTGCAGCTCTACAGCGTGTTCTTCAAGTTCCTCTTGAAGCACCGTTTGCAGAACCGGATCCAAAACCAACCCGACGAGTTCAACCCTTTTGGCGTCACCTCCCGACCCGAAGAAACCATCGTCGCACCCAATCCGGTATTTGACGACGGCGTCGCCACCAAGGACATCCACATCGACCCCCTCACTTCTCTTTCTATCCGAATCTTCCTCCCCGAGTCCGCCCTCGCCCCACCCGAACCCGCTTCCAAGCCTAGGGTTATCGCCGCACCGAAGCGATCCGATCTCAATAACGGGCCCGACCGAGCCGATCCGGTCCGGCAGAGCCTTAACAATTTACCGGCCACCCCTTCTCGGCGCAACAGCTATGGCAATCCGGTGGTGTCCAATTTGGCGAAAAGTGAGCAGAGACGGAACAGTTATGGGTGTAGCAATGACATGGAGGGATTGAATTTGATGGCCAATGCCGGCGCCGGAGTGTACAGAGGCTACTCGCCGGGCAATTCGACCAGGACAGGTCGAAAATTGCCGGTCATGTTGCAGTTTCACGGCGGCGGTTGGGTCAGTGGGAGTAATGATTCGGTGGCCAATGATATCTTCTGCAGGCGGATTGCGAAGCTCTGTGACGTCGTAGTATTGGCGGTGGGTTATCGGCTTGCCCCGGAGAATCGGTACCCTGCAGCATTTGAGGACGGGTTGAAGGTGTTGAATTGGCTTGGGAAGCAAGCGAATTTAGCTGAGTGTTGTAAGTCCATGGGAAGTGGAAAGGGAGCGCTCGGTGGCGTTACGGAGTTTAAGAAGACTGATTCTCATAGGCATATCGTGGACTCGTTCGGGGCGTCAATGGTTGAGCCCTGGTGTTCTCCTTGGTGTAAGCTGTGGTGCGAACATTGCGGACTATGTGGCCCGGAAAGCTGTAGAGGCAGGCAAGCTTTTGGACCCTGTGAAGGTGGTTGCACGGGTCCTGATGTACCCGTTCTTCATTGGAAGTGTTCCCACCCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

340

Amino Acids

36.92

Weight (kDa)

8.73

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 168 - 224 2.3e-06 Carboxylesterase family
BD-FAE PF20434 176 - 248 8.5e-08 BD-FAE
Abhydrolase_3 PF07859 183 - 248 5.4e-22 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27320 AT3G27320
fragaria_vesca FvH4_6g52540 FvH4_6g52540
malus_domestica MD09G1017600.v1.1 MD17G1018400.v1.1
prunus_persica Prupe.3G299300_v2.0.a1
pyrus_communis pycom111g01450 pycom17g01500
rosa_chinensis RchiOBHm_Chr2g0174131
rosa_laevigata RLG00000022249
rosa_multiflora Rmu_sc0003188.1_g000037 Rmu_sc0023199.1_g000004
rosa_roxburghii Rroxscaffold_2G00078430
rosa_rugosa Rorug02G0574900
rosa_samantha Rh2AG654500 Rh2BG665700 Rh2CG629900 Rh2DG680300
rosa_wichuraiana Rw2G053720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 642
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 2 cut(s) 482, 684
AciI CCGC 8 cut(s) 234, 255, 279, 564, 567, 619, 653, 906
AclWI GGATC 4 cut(s) 67, 80, 285, 317
AcoI YGGCCR 3 cut(s) 354, 471, 597
AcsI RAATTY 2 cut(s) 463, 742
AcyI GRCGYC 5 cut(s) 108, 165, 483, 639, 851
AfaI GTAC 3 cut(s) 494, 686, 984
AfeI AGCGCT 1 cut(s) 786
AfiI CCNNNNNNNGG 3 cut(s) 267, 366, 959
AgsI TTSAA 6 cut(s) 40, 52, 97, 463, 712, 721
AjnI CCWGG 2 cut(s) 521, 866
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 8 cut(s) 14, 22, 378, 631, 749, 889, 927, 944
AluI AGCT 8 cut(s) 14, 22, 378, 631, 749, 889, 927, 944
Alw26I GTCTC 1 cut(s) 415
AlwI GGATC 4 cut(s) 67, 80, 285, 317
Ama87I CYCGRG 1 cut(s) 227
Aor51HI AGCGCT 1 cut(s) 786
AoxI GGCC 5 cut(s) 308, 354, 471, 597, 916
ApaI GGGCCC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 19, 692
ApoI RAATTY 2 cut(s) 463, 742
Asp718I GGTACC 1 cut(s) 684
AspA2I CCTAGG 1 cut(s) 266
AspLEI GCGC 3 cut(s) 372, 485, 787
AspS9I GGNCC 6 cut(s) 308, 309, 328, 917, 950, 974
AsuC2I CCSGG 3 cut(s) 510, 674, 920
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 3 cut(s) 328, 950, 974
AvrII CCTAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 312
BalI TGGCCA 2 cut(s) 473, 599
BamHI GGATCC 1 cut(s) 72
BanI GGYRCC 2 cut(s) 482, 684
BanII GRGCYC 2 cut(s) 312, 867
BarI GAAGNNNNNNTAC 2 cut(s) 975, 1007
BbsI GAAGAC 1 cut(s) 818
BbvI GCAGC 2 cut(s) 31, 704
BccI CCATC 2 cut(s) 140, 463
BceAI ACGGC 2 cut(s) 178, 577
BciT130I CCWGG 2 cut(s) 523, 868
BcnI CCSGG 3 cut(s) 510, 674, 920
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 4 cut(s) 25, 613, 690, 928
BfoI RGCGCY 2 cut(s) 486, 788
BisI GCNGC 4 cut(s) 20, 279, 565, 693
BlnI CCTAGG 1 cut(s) 266
BlsI GCNGC 4 cut(s) 21, 280, 566, 694
Bme1390I CCNGG 5 cut(s) 510, 523, 674, 868, 920
Bme18I GGWCC 3 cut(s) 328, 950, 974
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 6 cut(s) 308, 309, 328, 917, 950, 974
BmiI GGNNCC 6 cut(s) 74, 310, 484, 686, 952, 975
BmrFI CCNGG 5 cut(s) 510, 523, 674, 868, 920
BpiI GAAGAC 1 cut(s) 818
BpuMI CCSGG 3 cut(s) 510, 674, 920
BsaHI GRCGYC 5 cut(s) 108, 165, 483, 639, 851
BsaJI CCNNGG 6 cut(s) 174, 266, 672, 765, 866, 878
BsaWI WCCGGW 4 cut(s) 69, 148, 325, 388
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 366, 959
Bse118I RCCGGY 3 cut(s) 352, 479, 541
Bse3DI GCAATG 2 cut(s) 451, 901
BseBI CCWGG 2 cut(s) 523, 868
BseDI CCNNGG 6 cut(s) 174, 266, 672, 765, 866, 878
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 3 cut(s) 267, 366, 959
BseMI GCAATG 2 cut(s) 451, 901
BseMII CTCAG 1 cut(s) 741
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 2 cut(s) 31, 704
BsgI GTGCAG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 310, 356, 473, 599, 918
BshNI GGYRCC 2 cut(s) 482, 684
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 267, 366, 959
BsmAI GTCTC 1 cut(s) 415
BsmBI CGTCTC 1 cut(s) 415
BsnI GGCC 5 cut(s) 310, 356, 473, 599, 918
BsoBI CYCGRG 1 cut(s) 227
Bsp120I GGGCCC 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 312, 867
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 4 cut(s) 72, 290, 295, 322
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 8 cut(s) 234, 255, 279, 564, 567, 619, 653, 906
BspANI GGCC 5 cut(s) 310, 356, 473, 599, 918
BspCNI CTCAG 1 cut(s) 742
BspLI GGNNCC 6 cut(s) 74, 310, 484, 686, 952, 975
BspMAI CTGCAG 2 cut(s) 617, 694
BspPI GGATC 4 cut(s) 67, 80, 285, 317
BspT107I GGYRCC 2 cut(s) 482, 684
BsrDI GCAATG 2 cut(s) 451, 901
BsrFI RCCGGY 3 cut(s) 352, 479, 541
BsrGI TGTACA 1 cut(s) 492
BssAI RCCGGY 3 cut(s) 352, 479, 541
BssECI CCNNGG 6 cut(s) 174, 266, 672, 765, 866, 878
BssMI GATC 4 cut(s) 72, 290, 295, 322
BssNI GRCGYC 5 cut(s) 108, 165, 483, 639, 851
BssT1I CCWWGG 4 cut(s) 174, 266, 765, 878
Bst2UI CCWGG 2 cut(s) 523, 868
Bst4CI ACNGT 2 cut(s) 59, 431
BstACI GRCGYC 5 cut(s) 108, 165, 483, 639, 851
BstAPI GCANNNNNTGC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 6 cut(s) 481, 617, 669, 738, 938, 942
BstDEI CTNAG 1 cut(s) 750
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 1 cut(s) 180
BstH2I RGCGCY 2 cut(s) 486, 788
BstHHI GCGC 3 cut(s) 372, 485, 787
BstKTI GATC 4 cut(s) 75, 293, 298, 325
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 4 cut(s) 72, 290, 295, 322
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 6 cut(s) 61, 733, 746, 903, 924, 933
BstNI CCWGG 2 cut(s) 523, 868
BstSCI CCNGG 5 cut(s) 508, 521, 672, 866, 918
BstSFI CTRYAG 4 cut(s) 25, 613, 690, 928
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 31, 704
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BsuRI GGCC 5 cut(s) 310, 356, 473, 599, 918
BtgI CCRYGG 1 cut(s) 765
BtsCI GGATG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 6 cut(s) 481, 617, 669, 738, 938, 942
CfoI GCGC 3 cut(s) 372, 485, 787
Cfr10I RCCGGY 3 cut(s) 352, 479, 541
Cfr13I GGNCC 6 cut(s) 308, 309, 328, 917, 950, 974
CpoI CGGWCCG 1 cut(s) 328
CseI GACGC 3 cut(s) 97, 154, 840
Csp6I GTAC 3 cut(s) 493, 685, 983
CspI CGGWCCG 1 cut(s) 328
CviAII CATG 3 cut(s) 451, 547, 766
CviQI GTAC 3 cut(s) 493, 685, 983
DdeI CTNAG 1 cut(s) 750
DinI GGCGCC 1 cut(s) 484
DpnI GATC 4 cut(s) 74, 292, 297, 324
DpnII GATC 4 cut(s) 72, 290, 295, 322
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 3 cut(s) 354, 471, 597
EciI GGCGGA 2 cut(s) 223, 634
Eco130I CCWWGG 4 cut(s) 174, 266, 765, 878
Eco24I GRGCYC 2 cut(s) 312, 867
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 3 cut(s) 328, 950, 974
Eco47III AGCGCT 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 974
EcoRII CCWGG 2 cut(s) 521, 866
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 4 cut(s) 174, 266, 765, 878
EcoT38I GRGCYC 2 cut(s) 312, 867
EgeI GGCGCC 1 cut(s) 484
EheI GGCGCC 1 cut(s) 484
ErhI CCWWGG 4 cut(s) 174, 266, 765, 878
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 3 cut(s) 454, 550, 769
FaiI YATR 8 cut(s) 381, 435, 452, 548, 767, 825, 831, 913
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FatI CATG 3 cut(s) 450, 546, 765
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 20, 279, 565, 693
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 2 cut(s) 312, 867
Fsp4HI GCNGC 4 cut(s) 20, 279, 565, 693
FspBI CTAG 1 cut(s) 267
GlaI GCGC 3 cut(s) 371, 484, 786
GluI GCNGC 4 cut(s) 20, 279, 565, 693
HaeII RGCGCY 2 cut(s) 486, 788
HaeIII GGCC 5 cut(s) 310, 356, 473, 599, 918
HgaI GACGC 3 cut(s) 97, 154, 840
HhaI GCGC 3 cut(s) 372, 485, 787
Hin1I GRCGYC 5 cut(s) 108, 165, 483, 639, 851
Hin1II CATG 3 cut(s) 454, 550, 769
Hin6I GCGC 3 cut(s) 370, 483, 785
HinP1I GCGC 3 cut(s) 370, 483, 785
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 6 cut(s) 216, 230, 590, 679, 818, 839
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 493, 838
Hpy188I TCNGA 3 cut(s) 215, 295, 1019
Hpy188III TCNNGA 3 cut(s) 49, 117, 977
Hpy8I GTNNAC 2 cut(s) 493, 838
Hpy99I CGWCG 5 cut(s) 92, 140, 164, 170, 644
HpyAV CCTTC 3 cut(s) 372, 706, 953
HpyCH4III ACNGT 2 cut(s) 59, 431
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 6 cut(s) 19, 64, 553, 615, 692, 969
HpyF10VI GCNNNNNNNGC 6 cut(s) 61, 733, 746, 903, 924, 933
HpyF3I CTNAG 1 cut(s) 750
HpySE526I ACGT 1 cut(s) 639
Hsp92I GRCGYC 5 cut(s) 108, 165, 483, 639, 851
Hsp92II CATG 3 cut(s) 454, 550, 769
HspAI GCGC 3 cut(s) 370, 483, 785
KasI GGCGCC 1 cut(s) 482
KpnI GGTACC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 479
KroNI GCCGGC 1 cut(s) 481
Kzo9I GATC 4 cut(s) 72, 290, 295, 322
LmnI GCTCC 1 cut(s) 782
Lsp1109I GCAGC 2 cut(s) 31, 704
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 3 cut(s) 109, 635, 796
MalI GATC 4 cut(s) 74, 292, 297, 324
MboI GATC 4 cut(s) 72, 290, 295, 322
MboII GAAGA 6 cut(s) 28, 137, 211, 601, 823, 983
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 312, 867
MlsI TGGCCA 2 cut(s) 473, 599
MluCI AATT 7 cut(s) 346, 400, 463, 514, 536, 721, 742
MluNI TGGCCA 2 cut(s) 473, 599
Mly113I GGCGCC 1 cut(s) 483
MlyI GAGTC 2 cut(s) 239, 833
MnlI CCTC 9 cut(s) 56, 124, 206, 233, 248, 448, 491, 694, 926
Mox20I TGGCCA 2 cut(s) 473, 599
MroNI GCCGGC 1 cut(s) 479
MscI TGGCCA 2 cut(s) 473, 599
MseI TTAA 2 cut(s) 342, 807
Msp20I TGGCCA 2 cut(s) 473, 599
MspR9I CCNGG 5 cut(s) 510, 523, 674, 868, 920
MvaI CCWGG 2 cut(s) 523, 868
MwoI GCNNNNNNNGC 6 cut(s) 61, 733, 746, 903, 924, 933
NaeI GCCGGC 1 cut(s) 481
NarI GGCGCC 1 cut(s) 483
NciI CCSGG 3 cut(s) 510, 674, 920
NcoI CCATGG 1 cut(s) 765
NdeII GATC 4 cut(s) 72, 290, 295, 322
NgoMIV GCCGGC 1 cut(s) 479
NlaIII CATG 3 cut(s) 454, 550, 769
NlaIV GGNNCC 6 cut(s) 74, 310, 484, 686, 952, 975
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 2 cut(s) 109, 635
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 848
PdiI GCCGGC 1 cut(s) 481
PfeI GAWTC 4 cut(s) 216, 590, 679, 818
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 4 cut(s) 21, 280, 566, 694
PleI GAGTC 2 cut(s) 238, 833
PluTI GGCGCC 1 cut(s) 486
PpsI GAGTC 2 cut(s) 238, 833
PpuMI RGGWCCY 1 cut(s) 974
Psp5II RGGWCCY 1 cut(s) 974
Psp6I CCWGG 2 cut(s) 521, 866
PspGI CCWGG 2 cut(s) 521, 866
PspN4I GGNNCC 6 cut(s) 74, 310, 484, 686, 952, 975
PspOMI GGGCCC 1 cut(s) 308
PspPI GGNCC 6 cut(s) 308, 309, 328, 917, 950, 974
PspPPI RGGWCCY 1 cut(s) 974
PstI CTGCAG 2 cut(s) 617, 694
PsuI RGATCY 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 3 cut(s) 494, 686, 984
RsaNI GTAC 3 cut(s) 493, 685, 983
Rsr2I CGGWCCG 1 cut(s) 328
RsrII CGGWCCG 1 cut(s) 328
SaqAI TTAA 2 cut(s) 342, 807
SatI GCNGC 4 cut(s) 20, 279, 565, 693
Sau3AI GATC 4 cut(s) 72, 290, 295, 322
Sau96I GGNCC 6 cut(s) 308, 309, 328, 917, 950, 974
SchI GAGTC 2 cut(s) 239, 833
ScrFI CCNGG 5 cut(s) 510, 523, 674, 868, 920
SduI GDGCHC 2 cut(s) 312, 867
SfcI CTRYAG 4 cut(s) 25, 613, 690, 928
SfoI GGCGCC 1 cut(s) 484
SinI GGWCC 3 cut(s) 328, 950, 974
Sse9I AATT 7 cut(s) 346, 400, 463, 514, 536, 721, 742
SsiI CCGC 8 cut(s) 234, 255, 279, 564, 567, 619, 653, 906
SspDI GGCGCC 1 cut(s) 482
SspMI CTAG 1 cut(s) 267
StyD4I CCNGG 5 cut(s) 508, 521, 672, 866, 918
StyI CCWWGG 4 cut(s) 174, 266, 765, 878
TaaI ACNGT 2 cut(s) 59, 431
TaiI ACGT 1 cut(s) 642
TaqI TCGA 3 cut(s) 189, 518, 532
TaqII GACCGA 1 cut(s) 330
TasI AATT 7 cut(s) 346, 400, 463, 514, 536, 721, 742
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 281, 567
TfiI GAWTC 4 cut(s) 216, 590, 679, 818
Tru1I TTAA 2 cut(s) 342, 807
Tru9I TTAA 2 cut(s) 342, 807
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 109, 635
TseI GCWGC 2 cut(s) 19, 692
Tsp45I GTSAC 2 cut(s) 109, 635
TspDTI ATGAA 1 cut(s) 983
TspGWI ACGGA 2 cut(s) 439, 815
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 3 cut(s) 328, 950, 974
XapI RAATTY 2 cut(s) 463, 742
XmaJI CCTAGG 1 cut(s) 266
XspI CTAG 1 cut(s) 267
ZraI GACGTC 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.