pycom111g02440
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0019552
Physical Location & Seq
Forward (+)
1448434 .. 1450934
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGGAGAGAGAAAAGGCAATACCAATAAAAATCGAGCCTTTCATCCGTTGCCTCTCTCATTTTGCTCCTTCCTCGTTCCTCCAGGTGAATTCGATTGAAAAAATGGGGCTTACGTTCACGAAGCTCTTCAGCCGGCTCTTTGCCAAGAAGGAGATGCGAATTCTGATGGTGGGTCTCGATGCAGCCGGTAAGACCACCATCTTGTACAAACTCAAGCTCGGAGAAATCGTCACCACCATCCCCACCATTGGATTTAATGTGGAGACAGTGGAATACAAGAACATCAGCTTCACTGTTTGGGATGTCGGGGGTCAAGACAAAATCCGTCCATTGTGGAGGCACTACTTCCAGAACACACAAGGTCTTATCTTTGTGGTTGATAGCAATGACAGGGATCGAGTTGTTGAGGCAAGGGATGAATTGCACAGGATGTTGAATGAGGATGAACTAAGGGATGCCGTTCTGCTTGTATTTGCCAACAAACAAGATCTTCCTAATGCAATGAATGCCGCCGAGATAACTGATAAGCTTGGTCTTCATTCTCTCCGTCAACGTCACTGGTACATCCAGAGCACTTGTGCAACCTCTGGAGAGGGTCTGTACGAGGGGTTGGACTGGCTTTCCAACAACATTGCTAGCAAGGCATGA

Protein Analysis

216

Amino Acids

24.61

Weight (kDa)

6.97

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 39 - 211 2.3e-81 ADP-ribosylation factor family
SRPRB PF09439 50 - 177 8e-13 Signal recognition particle receptor beta subunit
Roc PF08477 53 - 163 1.3e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 53 - 206 9.3e-12 Ras family
Gtr1_RagA PF04670 53 - 175 2.7e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 78 - 145 9.6e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019552)

Species Orthologous Gene IDs
prunus_persica Prupe.3G287000_v2.0.a1 Prupe.3G287000_v2.0.a1 Prupe.3G287000_v2.0.a1
pyrus_communis pycom111g02440
rosa_laevigata RLG00000022105
rosa_roxburghii Rroxscaffold_2G00079970
rosa_rugosa Rorug02G0558700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 510
AclWI GGATC 1 cut(s) 402
AcsI RAATTY 2 cut(s) 88, 159
AcuI CTGAAG 1 cut(s) 112
AfaI GTAC 3 cut(s) 206, 563, 602
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 2 cut(s) 98, 436
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 4 cut(s) 124, 217, 288, 529
AluI AGCT 4 cut(s) 124, 217, 288, 529
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 2 cut(s) 179, 257
AlwI GGATC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 2 cut(s) 88, 159
Asp700I GAANNNNTTC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 97, 223
AsuNHI GCTAGC 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 1 cut(s) 194
BccI CCATC 3 cut(s) 160, 206, 245
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 179, 257
BfaI CTAG 1 cut(s) 636
BglII AGATCT 1 cut(s) 487
BisI GCNGC 2 cut(s) 183, 510
BlsI GCNGC 2 cut(s) 184, 511
Bme1390I CCNGG 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 3 cut(s) 144, 169, 445
BmtI GCTAGC 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 2 cut(s) 65, 609
BpuEI CTTGAG 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 2 cut(s) 132, 185
Bse1I ACTGG 2 cut(s) 563, 620
Bse3DI GCAATG 3 cut(s) 391, 507, 630
BseBI CCWGG 1 cut(s) 83
BseGI GGATG 8 cut(s) 42, 237, 307, 421, 435, 448, 460, 564
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMI GCAATG 3 cut(s) 391, 507, 630
BseNI ACTGG 2 cut(s) 563, 620
BseXI GCAGC 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 2 cut(s) 133, 186
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 179, 257
BsmI GAATGC 1 cut(s) 511
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 575
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 394, 487
BspACI CCGC 1 cut(s) 510
BspOI GCTAGC 1 cut(s) 639
BspPI GGATC 1 cut(s) 402
BspQI GCTCTTC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 3 cut(s) 391, 507, 630
BsrFI RCCGGY 2 cut(s) 132, 185
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 563, 620
BssAI RCCGGY 2 cut(s) 132, 185
BssMI GATC 2 cut(s) 394, 487
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 2 cut(s) 268, 295
Bst6I CTCTTC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 134, 637
BstDEI CTNAG 1 cut(s) 449
BstF5I GGATG 8 cut(s) 42, 237, 307, 421, 435, 448, 460, 564
BstKTI GATC 2 cut(s) 397, 490
BstMAI GTCTC 2 cut(s) 179, 257
BstMBI GATC 2 cut(s) 394, 487
BstMWI GCNNNNNNNGC 2 cut(s) 506, 641
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BtsCI GGATG 8 cut(s) 42, 237, 307, 421, 435, 448, 460, 564
BtsIMutI CAGTG 3 cut(s) 273, 291, 556
Cac8I GCNNGC 2 cut(s) 134, 637
Cfr10I RCCGGY 2 cut(s) 132, 185
Csp6I GTAC 3 cut(s) 205, 562, 601
CviAII CATG 1 cut(s) 645
CviQI GTAC 3 cut(s) 205, 562, 601
DdeI CTNAG 1 cut(s) 449
DpnI GATC 2 cut(s) 396, 489
DpnII GATC 2 cut(s) 394, 487
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 112
EcoRI GAATTC 2 cut(s) 88, 159
EcoRII CCWGG 1 cut(s) 81
FaeI CATG 1 cut(s) 648
FaiI YATR 1 cut(s) 646
FatI CATG 1 cut(s) 644
Fnu4HI GCNGC 2 cut(s) 183, 510
FokI GGATG 8 cut(s) 29, 224, 314, 428, 442, 455, 467, 551
Fsp4HI GCNGC 2 cut(s) 183, 510
FspBI CTAG 1 cut(s) 636
GluI GCNGC 2 cut(s) 183, 510
GsuI CTGGAG 2 cut(s) 65, 609
HapII CCGG 2 cut(s) 133, 186
Hin1II CATG 1 cut(s) 648
HincII GTYRAC 1 cut(s) 551
HindII GTYRAC 1 cut(s) 551
HindIII AAGCTT 1 cut(s) 527
HpaII CCGG 2 cut(s) 133, 186
HphI GGTGA 2 cut(s) 97, 223
Hpy166II GTNNAC 2 cut(s) 117, 551
Hpy188I TCNGA 2 cut(s) 165, 221
Hpy188III TCNNGA 6 cut(s) 118, 176, 314, 349, 568, 588
Hpy8I GTNNAC 2 cut(s) 117, 551
HpyAV CCTTC 2 cut(s) 78, 142
HpyCH4III ACNGT 2 cut(s) 268, 295
HpyCH4IV ACGT 2 cut(s) 113, 553
HpyCH4V TGCA 4 cut(s) 182, 424, 500, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 641
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 2 cut(s) 113, 553
Hsp92II CATG 1 cut(s) 648
KroI GCCGGC 1 cut(s) 132
KroNI GCCGGC 1 cut(s) 134
Kzo9I GATC 2 cut(s) 394, 487
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 70
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 3 cut(s) 144, 169, 445
MaeI CTAG 1 cut(s) 636
MaeII ACGT 2 cut(s) 113, 553
MaeIII GTNAC 2 cut(s) 229, 554
MalI GATC 2 cut(s) 396, 489
MboI GATC 2 cut(s) 394, 487
MboII GAAGA 3 cut(s) 118, 482, 527
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 575
MluCI AATT 3 cut(s) 88, 159, 419
MmeI TCCRAC 2 cut(s) 591, 648
MnlI CCTC 9 cut(s) 62, 82, 89, 330, 400, 433, 586, 595, 598
MroNI GCCGGC 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 1 cut(s) 255
MspI CCGG 2 cut(s) 133, 186
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 506, 641
NaeI GCCGGC 1 cut(s) 134
NdeII GATC 2 cut(s) 394, 487
NgoMIV GCCGGC 1 cut(s) 132
NheI GCTAGC 1 cut(s) 635
NlaIII CATG 1 cut(s) 648
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 2 cut(s) 229, 554
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 511
PdiI GCCGGC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 125
PkrI GCNGC 2 cut(s) 184, 511
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PsuI RGATCY 1 cut(s) 487
RsaI GTAC 3 cut(s) 206, 563, 602
RsaNI GTAC 3 cut(s) 205, 562, 601
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 2 cut(s) 183, 510
Sau3AI GATC 2 cut(s) 394, 487
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 575
SetI ASST 9 cut(s) 87, 116, 126, 219, 290, 364, 531, 556, 587
SfaNI GCATC 3 cut(s) 144, 169, 445
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 3 cut(s) 88, 159, 419
SsiI CCGC 1 cut(s) 510
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 268, 295
TaiI ACGT 2 cut(s) 116, 556
TaqI TCGA 4 cut(s) 33, 92, 177, 397
TasI AATT 3 cut(s) 88, 159, 419
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 3 cut(s) 273, 298, 563
TseFI GTSAC 2 cut(s) 229, 554
TseI GCWGC 1 cut(s) 182
Tsp45I GTSAC 2 cut(s) 229, 554
TspDTI ATGAA 5 cut(s) 31, 432, 459, 518, 527
TspGWI ACGGA 3 cut(s) 35, 314, 536
TspRI CASTG 3 cut(s) 273, 298, 563
XapI RAATTY 2 cut(s) 88, 159
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.