pycom111g03100

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0029393
Physical Location & Seq
Reverse (-)
1885525 .. 1886244
720 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03100.1

Sequence Viewer

Length: 720 bp
ATGGAACTGCGGAATGGTAAACAAACCCAGCAGAAGCAGATGAACAGTCTCTCAATGCGCTCGAACAGATGGCGCACTCGAAGCGGAAGACTTGGAATCCCGCTCACTCTTCCCGCATTCTTTCCCTTTCCTAGAAGCAAAAGCAGCCATGAACCTGTCGTTCCAGCTGATGCCATTACCCGCCAGCAACGACGGCTGAGCTGCCGTTTGCTTCCTGCAAACATGGTCGGCCGGGAACCGGTGCTTGAGGCAGACCTTGATCTGGCAGGTTTTGCAGGTGCTGGTATTAGAAAACGTGAGAGTCTCCTTGCACCGGCGAACGGGACAGGTGGGTTTCTTCTTGTTTTTGGGGTCGCAATTCCCAGACTTGGCGTGCTTCTCCAACAGCAATTTATGGTCTTGTTCGTCGTCGCAGCCGGTGGTTTCTATGGAGGCGGAGCAGATTTCGCAAACCACCACCTTTCTGCTGTTGTGGTCCGATTTGGGGCACTCGTGGGACTTGTAAGACCGATGCTCAACGCAGAAAACCTTACGGCAACCGTCGCACTGGAAGGGGAGGAAATCGAGTTGGTGGCAATCGGAGAGCTGGCAGTGCCGACCCAAATCCGGAAAAGCTTCTGTTCCCCCCGTCATCCTGATCGTAGTTTGATTTTTCAGTCTGTTAAATTTTGCAATGAAACAATTCGTAAGCAAAAGGGGGAATTTAAATTTTATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

240

Amino Acids

26.27

Weight (kDa)

10.36

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029393)

Species Orthologous Gene IDs
pyrus_communis pycom111g03100
rosa_samantha Rh2AG619700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 2 cut(s) 257, 266
AccBSI CCGCTC 1 cut(s) 103
AccIII TCCGGA 1 cut(s) 606
AciI CCGC 6 cut(s) 10, 84, 101, 114, 181, 435
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 3 cut(s) 665, 701, 707
AfiI CCNNNNNNNGG 7 cut(s) 238, 262, 313, 320, 368, 484, 606
AgeI ACCGGT 1 cut(s) 238
AluBI AGCT 4 cut(s) 167, 201, 586, 615
AluI AGCT 4 cut(s) 167, 201, 586, 615
Alw26I GTCTC 2 cut(s) 53, 308
AlwNI CAGNNNCTG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 606
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 3 cut(s) 144, 201, 413
ApoI RAATTY 3 cut(s) 665, 701, 707
AsiGI ACCGGT 1 cut(s) 238
Asp700I GAANNNNTTC 2 cut(s) 614, 681
AspLEI GCGC 2 cut(s) 60, 75
AspS9I GGNCC 1 cut(s) 475
AsuC2I CCSGG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 475
BaeGI GKGCMC 1 cut(s) 490
BauI CACGAG 1 cut(s) 491
BbsI GAAGAC 1 cut(s) 94
BbvI GCAGC 3 cut(s) 156, 188, 425
BccI CCATC 1 cut(s) 63
BceAI ACGGC 3 cut(s) 189, 209, 549
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 2 cut(s) 53, 308
BfaI CTAG 1 cut(s) 132
BfuAI ACCTGC 2 cut(s) 257, 266
BisI GCNGC 3 cut(s) 145, 202, 414
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 3 cut(s) 146, 203, 415
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 2 cut(s) 160, 501
BpiI GAAGAC 1 cut(s) 94
Bpu1102I GCTNAGC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 233
BsaWI WCCGGW 2 cut(s) 238, 606
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 262, 313, 320, 368, 484, 606
Bse118I RCCGGY 3 cut(s) 238, 313, 416
Bse1I ACTGG 1 cut(s) 552
Bse3DI GCAATG 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 606
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 7 cut(s) 238, 262, 313, 320, 368, 484, 606
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 552
BseSI GKGCMC 1 cut(s) 490
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 156, 188, 425
BseYI CCCAGC 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 231
BshTI ACCGGT 1 cut(s) 238
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 5 cut(s) 232, 239, 314, 417, 607
BslFI GGGAC 2 cut(s) 337, 510
BslI CCNNNNNNNGG 7 cut(s) 238, 262, 313, 320, 368, 484, 606
BsmAI GTCTC 2 cut(s) 53, 308
BsmFI GGGAC 2 cut(s) 337, 510
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 490
Bsp13I TCCGGA 1 cut(s) 606
Bsp143I GATC 2 cut(s) 259, 637
Bsp1720I GCTNAGC 1 cut(s) 197
BspACI CCGC 6 cut(s) 10, 84, 101, 114, 181, 435
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 606
BspLI GGNNCC 1 cut(s) 237
BspMI ACCTGC 2 cut(s) 257, 266
BsrBI CCGCTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 3 cut(s) 238, 313, 416
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 3 cut(s) 238, 313, 416
BssMI GATC 2 cut(s) 259, 637
BssSI CACGAG 1 cut(s) 491
Bst2BI CACGAG 1 cut(s) 491
Bst4CI ACNGT 2 cut(s) 47, 541
Bst6I CTCTTC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 3 cut(s) 185, 374, 588
BstDEI CTNAG 1 cut(s) 197
BstF5I GGATG 1 cut(s) 631
BstHHI GCGC 2 cut(s) 60, 75
BstKTI GATC 2 cut(s) 262, 640
BstMAI GTCTC 2 cut(s) 53, 308
BstMBI GATC 2 cut(s) 259, 637
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 7 cut(s) 81, 144, 193, 272, 446, 542, 592
BstSCI CCNGG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 156, 188, 425
BstV2I GAAGAC 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 1 cut(s) 231
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 545, 597
BveI ACCTGC 2 cut(s) 257, 266
Cac8I GCNNGC 3 cut(s) 185, 374, 588
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 2 cut(s) 60, 75
Cfr10I RCCGGY 3 cut(s) 238, 313, 416
Cfr13I GGNCC 1 cut(s) 475
CspAI ACCGGT 1 cut(s) 238
CviAII CATG 2 cut(s) 149, 223
CviJI RGCY 8 cut(s) 147, 167, 196, 201, 231, 416, 586, 615
CviKI_1 RGCY 8 cut(s) 147, 167, 196, 201, 231, 416, 586, 615
DdeI CTNAG 1 cut(s) 197
DpnI GATC 2 cut(s) 261, 639
DpnII GATC 2 cut(s) 259, 637
DraI TTTAAA 1 cut(s) 706
EaeI YGGCCR 1 cut(s) 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
EciI GGCGGA 1 cut(s) 450
EclXI CGGCCG 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 475
Eco52I CGGCCG 1 cut(s) 229
FaeI CATG 2 cut(s) 152, 226
FaiI YATR 5 cut(s) 150, 224, 395, 429, 718
FaqI GGGAC 2 cut(s) 337, 510
FatI CATG 2 cut(s) 148, 222
FauI CCCGC 3 cut(s) 108, 121, 188
Fnu4HI GCNGC 3 cut(s) 145, 202, 414
FokI GGATG 1 cut(s) 618
Fsp4HI GCNGC 3 cut(s) 145, 202, 414
FspBI CTAG 1 cut(s) 132
GlaI GCGC 2 cut(s) 59, 74
GluI GCNGC 3 cut(s) 145, 202, 414
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 231
HapII CCGG 5 cut(s) 232, 239, 314, 417, 607
HhaI GCGC 2 cut(s) 60, 75
Hin1II CATG 2 cut(s) 152, 226
Hin6I GCGC 2 cut(s) 58, 73
HinP1I GCGC 2 cut(s) 58, 73
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 2 cut(s) 96, 301
HpaII CCGG 5 cut(s) 232, 239, 314, 417, 607
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 479, 581
Hpy188III TCNNGA 2 cut(s) 607, 635
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 4 cut(s) 195, 410, 413, 545
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 2 cut(s) 47, 541
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 4 cut(s) 218, 275, 311, 672
HpyF10VI GCNNNNNNNGC 7 cut(s) 81, 144, 193, 272, 446, 542, 592
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 152, 226
HspAI GCGC 2 cut(s) 58, 73
Kpn2I TCCGGA 1 cut(s) 606
Kzo9I GATC 2 cut(s) 259, 637
LmnI GCTCC 1 cut(s) 437
Lsp1109I GCAGC 3 cut(s) 156, 188, 425
LweI GCATC 2 cut(s) 160, 501
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 295
MalI GATC 2 cut(s) 261, 639
MbiI CCGCTC 1 cut(s) 103
MboI GATC 2 cut(s) 259, 637
MboII GAAGA 3 cut(s) 99, 101, 329
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 6 cut(s) 357, 389, 665, 681, 701, 707
MlyI GAGTC 1 cut(s) 310
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 3 cut(s) 241, 425, 550
MroI TCCGGA 1 cut(s) 606
MroXI GAANNNNTTC 2 cut(s) 614, 681
MseI TTAA 2 cut(s) 663, 705
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 5 cut(s) 232, 239, 314, 417, 607
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 116
MwoI GCNNNNNNNGC 7 cut(s) 81, 144, 193, 272, 446, 542, 592
NciI CCSGG 1 cut(s) 233
NdeII GATC 2 cut(s) 259, 637
NlaIII CATG 2 cut(s) 152, 226
NlaIV GGNNCC 1 cut(s) 237
PaqCI CACCTGC 1 cut(s) 266
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 2 cut(s) 614, 681
PfeI GAWTC 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 3 cut(s) 146, 203, 415
PleI GAGTC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 167
SaqAI TTAA 2 cut(s) 663, 705
SatI GCNGC 3 cut(s) 145, 202, 414
Sau3AI GATC 2 cut(s) 259, 637
Sau96I GGNCC 1 cut(s) 475
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 490
SfaNI GCATC 2 cut(s) 160, 501
SgrAI CRCCGGYG 1 cut(s) 313
SinI GGWCC 1 cut(s) 475
SmiI ATTTAAAT 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 6 cut(s) 357, 389, 665, 681, 701, 707
SsiI CCGC 6 cut(s) 10, 84, 101, 114, 181, 435
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 231
SwaI ATTTAAAT 1 cut(s) 706
TaaI ACNGT 2 cut(s) 47, 541
TaiI ACGT 1 cut(s) 298
TaqI TCGA 3 cut(s) 62, 79, 564
TaqII GACCGA 1 cut(s) 523
TasI AATT 6 cut(s) 357, 389, 665, 681, 701, 707
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 2 cut(s) 663, 705
Tru9I TTAA 2 cut(s) 663, 705
TscAI CASTG 2 cut(s) 552, 597
TseI GCWGC 3 cut(s) 144, 201, 413
TspDTI ATGAA 3 cut(s) 56, 165, 690
TspRI CASTG 2 cut(s) 552, 597
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 3 cut(s) 665, 701, 707
XmnI GAANNNNTTC 2 cut(s) 614, 681
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.