pycom11g07840

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0024678
Physical Location & Seq
Forward (+)
5978043 .. 5978282
240 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 240 bp
ATGGCTCTAGTGAGACCCGGATCCGGAAACCAACCCCCAAAGCTCCGATGGGGAGAGCTCGAGGAGGACGACGGCGAAGACCTCGACTTTCTCCTGCCGCCGAGGCAGGTGATTGGGCCCGACGAGGACGGGATCAAGAGGGTGATCGAATACAAGTTCAACGAGGATGGAAATAAGGTCAACCGATCTGATCACGACCATTACCCGGGTTCGGAGACTCGCCCAGGCCCGGCTCAGTAA

Protein Analysis

80

Amino Acids

8.9

Weight (kDa)

4.64

Isoelectric Point (pI)

67.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024678)

Species Orthologous Gene IDs
pyrus_communis pycom11g07840
rosa_multiflora Rmu_co7962524.1_g000001 Rmu_ssc0000249.1_g000026

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 97
AccIII TCCGGA 1 cut(s) 23
AciI CCGC 1 cut(s) 98
AclWI GGATC 3 cut(s) 15, 28, 140
AfiI CCNNNNNNNGG 4 cut(s) 23, 205, 211, 229
AgsI TTSAA 1 cut(s) 160
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 2 cut(s) 43, 58
AluI AGCT 2 cut(s) 43, 58
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 7, 209
AlwI GGATC 3 cut(s) 15, 28, 140
Ama87I CYCGRG 2 cut(s) 59, 205
Aor13HI TCCGGA 1 cut(s) 23
AoxI GGCC 2 cut(s) 116, 226
ApaI GGGCCC 1 cut(s) 120
AspS9I GGNCC 3 cut(s) 116, 117, 227
AsuC2I CCSGG 4 cut(s) 18, 206, 207, 230
AsuHPI GGTGA 2 cut(s) 121, 154
AvaI CYCGRG 2 cut(s) 59, 205
BaeGI GKGCMC 1 cut(s) 120
BamHI GGATCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 60, 120
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 60
BccI CCATC 2 cut(s) 42, 161
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 225
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 4 cut(s) 18, 206, 207, 230
BcoDI GTCTC 2 cut(s) 7, 209
BfaI CTAG 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 97
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 5 cut(s) 18, 206, 207, 225, 230
BmeT110I CYCGRG 2 cut(s) 59, 205
BmgT120I GGNCC 3 cut(s) 116, 117, 227
BmiI GGNNCC 2 cut(s) 22, 118
BmrFI CCNGG 5 cut(s) 18, 206, 207, 225, 230
BpiI GAAGAC 1 cut(s) 84
BpuMI CCSGG 4 cut(s) 18, 206, 207, 230
BsaI GGTCTC 1 cut(s) 7
BsaJI CCNNGG 3 cut(s) 101, 205, 223
BsaWI WCCGGW 1 cut(s) 23
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 205, 211, 229
BseAI TCCGGA 1 cut(s) 23
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 101, 205, 223
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 4 cut(s) 23, 205, 211, 229
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 120
BshFI GGCC 2 cut(s) 118, 228
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 2 cut(s) 59, 205
BsiSI CCGG 4 cut(s) 18, 24, 206, 230
BslI CCNNNNNNNGG 4 cut(s) 23, 205, 211, 229
BsmAI GTCTC 2 cut(s) 7, 209
BsnI GGCC 2 cut(s) 118, 228
Bso31I GGTCTC 1 cut(s) 7
BsoBI CYCGRG 2 cut(s) 59, 205
Bsp120I GGGCCC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 60, 120
Bsp13I TCCGGA 1 cut(s) 23
Bsp143I GATC 5 cut(s) 20, 132, 144, 185, 190
BspACI CCGC 1 cut(s) 98
BspANI GGCC 2 cut(s) 118, 228
BspEI TCCGGA 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 22, 118
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 3 cut(s) 15, 28, 140
BspTNI GGTCTC 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 101, 205, 223
BssMI GATC 5 cut(s) 20, 132, 144, 185, 190
Bst2UI CCWGG 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 5 cut(s) 23, 135, 147, 188, 193
BstMAI GTCTC 2 cut(s) 7, 209
BstMBI GATC 5 cut(s) 20, 132, 144, 185, 190
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 5 cut(s) 16, 204, 205, 223, 228
BstSLI GKGCMC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BsuRI GGCC 2 cut(s) 118, 228
BtsCI GGATG 1 cut(s) 172
BveI ACCTGC 1 cut(s) 97
Cfr13I GGNCC 3 cut(s) 116, 117, 227
Cfr9I CCCGGG 1 cut(s) 205
CviJI RGCY 6 cut(s) 5, 43, 58, 118, 228, 233
CviKI_1 RGCY 6 cut(s) 5, 43, 58, 118, 228, 233
DdeI CTNAG 1 cut(s) 234
DpnI GATC 5 cut(s) 22, 134, 146, 187, 192
DpnII GATC 5 cut(s) 20, 132, 144, 185, 190
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 60, 120
Eco31I GGTCTC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 58
Eco88I CYCGRG 2 cut(s) 59, 205
EcoICRI GAGCTC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 60, 120
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 60, 120
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 8
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 2 cut(s) 118, 228
HapII CCGG 4 cut(s) 18, 24, 206, 230
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 1 cut(s) 217
HpaII CCGG 4 cut(s) 18, 24, 206, 230
HphI GGTGA 2 cut(s) 121, 154
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 3 cut(s) 47, 190, 214
Hpy188III TCNNGA 3 cut(s) 24, 136, 194
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 2 cut(s) 74, 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
HpyF3I CTNAG 1 cut(s) 234
Kpn2I TCCGGA 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 5 cut(s) 20, 132, 144, 185, 190
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 6 cut(s) 31, 37, 92, 107, 210, 219
MaeI CTAG 1 cut(s) 8
MalI GATC 5 cut(s) 22, 134, 146, 187, 192
MboI GATC 5 cut(s) 20, 132, 144, 185, 190
MboII GAAGA 1 cut(s) 89
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 2 cut(s) 60, 120
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 7 cut(s) 55, 58, 92, 96, 118, 132, 157
MroI TCCGGA 1 cut(s) 23
MspI CCGG 4 cut(s) 18, 24, 206, 230
MspR9I CCNGG 5 cut(s) 18, 206, 207, 225, 230
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 1 cut(s) 103
NciI CCSGG 4 cut(s) 18, 206, 207, 230
NdeII GATC 5 cut(s) 20, 132, 144, 185, 190
NlaIV GGNNCC 2 cut(s) 22, 118
NmeAIII GCCGAG 1 cut(s) 126
PaeR7I CTCGAG 1 cut(s) 59
PaqCI CACCTGC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 22, 118
PspOMI GGGCCC 1 cut(s) 116
PspPI GGNCC 3 cut(s) 116, 117, 227
PspXI VCTCGAGB 1 cut(s) 59
PsuI RGATCY 1 cut(s) 20
SacI GAGCTC 1 cut(s) 60
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 5 cut(s) 20, 132, 144, 185, 190
Sau96I GGNCC 3 cut(s) 116, 117, 227
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 5 cut(s) 18, 206, 207, 225, 230
SduI GDGCHC 2 cut(s) 60, 120
SetI ASST 5 cut(s) 45, 60, 84, 111, 180
Sfr274I CTCGAG 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 59
SmaI CCCGGG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SsiI CCGC 1 cut(s) 98
SspMI CTAG 1 cut(s) 8
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 5 cut(s) 16, 204, 205, 223, 228
TaqI TCGA 3 cut(s) 60, 84, 147
TauI GCSGC 1 cut(s) 100
TspMI CCCGGG 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 45
XhoI CTCGAG 1 cut(s) 59
XmaI CCCGGG 1 cut(s) 205
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.