Rmu_ssc0000249.1_g000026

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000249.1
OG0024678
Physical Location & Seq
Reverse (-)
118737 .. 121892
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000249.1_g000026.1.cds

Sequence Viewer

Length: 492 bp
atggctctagtgcgaccaggctcagcgaaccaaccggcgaagatccgatggggcgagctcgacgaggacgacggcgaggacctcgacttcctcctgccgccgaagcaggtgattggccccgacgagaacgggatcaagaaggtgattgagtacaaattcaacgacgagggcaataaggtcaagatcaccaccatcactcgaacccggaaactcgccaatgctaggctcagcaagcgcgccgtcgagcgccgctcctggcccaaattcggcgacgccgttcatgaggacgtcggcgccaggctcaccatggtctccactgaagagatcctcctcgaacgccctagggctcttggagccttggcagaatctgaagacaatagaccatcgaagtgtttgaatcctgaccgattgaagcttgccagatctgctggaagtctcaggtatacctgcaatgccaatccactgcaccaggaacttgctgtggttggttga

Protein Analysis

163

Amino Acids

18.16

Weight (kDa)

8.56

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024678)

Species Orthologous Gene IDs
pyrus_communis pycom11g07840
rosa_multiflora Rmu_co7962524.1_g000001 Rmu_ssc0000249.1_g000026

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 97
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 2 cut(s) 97, 455
AccB1I GGYRCC 1 cut(s) 293
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 443
AccII CGCG 1 cut(s) 237
AciI CCGC 2 cut(s) 98, 250
AclWI GGATC 3 cut(s) 37, 140, 319
AcsI RAATTY 2 cut(s) 155, 263
AcuI CTGAAG 2 cut(s) 339, 390
AcyI GRCGYC 3 cut(s) 273, 288, 294
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 222, 266
AgsI TTSAA 3 cut(s) 160, 397, 412
AjnI CCWGG 4 cut(s) 16, 254, 296, 468
AluBI AGCT 2 cut(s) 58, 415
AluI AGCT 2 cut(s) 58, 415
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 316, 440
AlwI GGATC 3 cut(s) 37, 140, 319
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 2 cut(s) 115, 257
ApoI RAATTY 2 cut(s) 155, 263
AspA2I CCTAGG 1 cut(s) 341
AspLEI GCGC 4 cut(s) 237, 239, 249, 296
AspS9I GGNCC 3 cut(s) 79, 116, 258
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 4 cut(s) 121, 154, 178, 295
AvaII GGWCC 1 cut(s) 79
AvrII CCTAGG 1 cut(s) 341
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 2 cut(s) 60, 349
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 60
BccI CCATC 3 cut(s) 42, 200, 391
BceAI ACGGC 3 cut(s) 88, 224, 260
BciT130I CCWGG 4 cut(s) 18, 256, 298, 470
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 316, 440
BfaI CTAG 3 cut(s) 8, 222, 342
BfoI RGCGCY 2 cut(s) 250, 297
BfuAI ACCTGC 2 cut(s) 97, 455
BglII AGATCT 1 cut(s) 422
BisI GCNGC 2 cut(s) 98, 250
BlnI CCTAGG 1 cut(s) 341
BlpI GCTNAGC 2 cut(s) 22, 227
BlsI GCNGC 2 cut(s) 99, 251
Bme1390I CCNGG 5 cut(s) 18, 205, 256, 298, 470
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 79, 116, 258
BmiI GGNNCC 3 cut(s) 118, 295, 355
BmrFI CCNGG 5 cut(s) 18, 205, 256, 298, 470
BpiI GAAGAC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 236, 268
Bpu1102I GCTNAGC 2 cut(s) 22, 227
BpuMI CCSGG 1 cut(s) 205
BsaHI GRCGYC 3 cut(s) 273, 288, 294
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 306, 341, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 266
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 457
BseBI CCWGG 4 cut(s) 18, 256, 298, 470
BseDI CCNNGG 3 cut(s) 306, 341, 357
BseLI CCNNNNNNNGG 2 cut(s) 222, 266
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 3 cut(s) 36, 241, 451
BsePI GCGCGC 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 320
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 117, 259
BshNI GGYRCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 2 cut(s) 35, 205
BslI CCNNNNNNNGG 2 cut(s) 222, 266
BsmAI GTCTC 2 cut(s) 316, 440
BsnI GGCC 2 cut(s) 117, 259
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 2 cut(s) 60, 349
Bsp143I GATC 5 cut(s) 42, 132, 183, 324, 422
Bsp1720I GCTNAGC 2 cut(s) 22, 227
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 2 cut(s) 98, 250
BspANI GGCC 2 cut(s) 117, 259
BspCNI CTCAG 3 cut(s) 35, 240, 450
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 3 cut(s) 118, 295, 355
BspMI ACCTGC 2 cut(s) 97, 455
BspPI GGATC 3 cut(s) 37, 140, 319
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 457
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 306, 341, 357
BssHII GCGCGC 1 cut(s) 235
BssMI GATC 5 cut(s) 42, 132, 183, 324, 422
BssNAI GTATAC 1 cut(s) 444
BssNI GRCGYC 3 cut(s) 273, 288, 294
BssT1I CCWWGG 3 cut(s) 306, 341, 357
Bst1107I GTATAC 1 cut(s) 444
Bst2UI CCWGG 4 cut(s) 18, 256, 298, 470
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 3 cut(s) 273, 288, 294
BstC8I GCNNGC 4 cut(s) 56, 233, 237, 417
BstDEI CTNAG 3 cut(s) 22, 227, 437
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 2 cut(s) 250, 297
BstHHI GCGC 4 cut(s) 237, 239, 249, 296
BstKTI GATC 5 cut(s) 45, 135, 186, 327, 425
BstMAI GTCTC 2 cut(s) 316, 440
BstMBI GATC 5 cut(s) 42, 132, 183, 324, 422
BstMWI GCNNNNNNNGC 4 cut(s) 103, 232, 353, 425
BstNI CCWGG 4 cut(s) 18, 256, 298, 470
BstSCI CCNGG 5 cut(s) 16, 203, 254, 296, 468
BstUI CGCG 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 3 cut(s) 42, 324, 422
BstYI RGATCY 3 cut(s) 42, 324, 422
BstZ17I GTATAC 1 cut(s) 444
BsuRI GGCC 2 cut(s) 117, 259
BtgI CCRYGG 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 2 cut(s) 315, 461
BveI ACCTGC 2 cut(s) 97, 455
Cac8I GCNNGC 4 cut(s) 56, 233, 237, 417
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 1 cut(s) 280
CfoI GCGC 4 cut(s) 237, 239, 249, 296
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 79, 116, 258
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 151
CviAII CATG 2 cut(s) 281, 307
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 22, 227, 437
DinI GGCGCC 1 cut(s) 295
DpnI GATC 5 cut(s) 44, 134, 185, 326, 424
DpnII GATC 5 cut(s) 42, 132, 183, 324, 422
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 3 cut(s) 306, 341, 357
Eco24I GRGCYC 2 cut(s) 60, 349
Eco31I GGTCTC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 339, 390
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 4 cut(s) 16, 254, 296, 468
EcoT14I CCWWGG 3 cut(s) 306, 341, 357
EcoT38I GRGCYC 2 cut(s) 60, 349
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 3 cut(s) 306, 341, 357
FaeI CATG 2 cut(s) 284, 310
FaiI YATR 3 cut(s) 282, 308, 444
FatI CATG 2 cut(s) 280, 306
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 2 cut(s) 98, 250
FriOI GRGCYC 2 cut(s) 60, 349
Fsp4HI GCNGC 2 cut(s) 98, 250
FspBI CTAG 3 cut(s) 8, 222, 342
GlaI GCGC 4 cut(s) 236, 238, 248, 295
GluI GCNGC 2 cut(s) 98, 250
HaeII RGCGCY 2 cut(s) 250, 297
HaeIII GGCC 2 cut(s) 117, 259
HapII CCGG 2 cut(s) 35, 205
HgaI GACGC 1 cut(s) 281
HhaI GCGC 4 cut(s) 237, 239, 249, 296
Hin1I GRCGYC 3 cut(s) 273, 288, 294
Hin1II CATG 2 cut(s) 284, 310
Hin6I GCGC 4 cut(s) 235, 237, 247, 294
HinP1I GCGC 4 cut(s) 235, 237, 247, 294
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 2 cut(s) 365, 397
HpaII CCGG 2 cut(s) 35, 205
HphI GGTGA 4 cut(s) 121, 154, 178, 295
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 2 cut(s) 47, 370
Hpy188III TCNNGA 4 cut(s) 136, 181, 281, 401
Hpy8I GTNNAC 1 cut(s) 444
Hpy99I CGWCG 7 cut(s) 65, 74, 125, 167, 245, 275, 293
HpyAV CCTTC 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 2 cut(s) 450, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 232, 353, 425
HpyF3I CTNAG 3 cut(s) 22, 227, 437
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 3 cut(s) 273, 288, 294
Hsp92II CATG 2 cut(s) 284, 310
HspAI GCGC 4 cut(s) 235, 237, 247, 294
KasI GGCGCC 1 cut(s) 293
Kzo9I GATC 5 cut(s) 42, 132, 183, 324, 422
LmnI GCTCC 2 cut(s) 257, 353
MaeI CTAG 3 cut(s) 8, 222, 342
MaeII ACGT 1 cut(s) 288
MalI GATC 5 cut(s) 44, 134, 185, 326, 424
MbiI CCGCTC 1 cut(s) 252
MboI GATC 5 cut(s) 42, 132, 183, 324, 422
MboII GAAGA 3 cut(s) 52, 332, 383
MflI RGATCY 3 cut(s) 42, 324, 422
MhlI GDGCHC 2 cut(s) 60, 349
MluCI AATT 2 cut(s) 155, 263
Mly113I GGCGCC 1 cut(s) 294
MnlI CCTC 8 cut(s) 58, 70, 92, 101, 160, 277, 338, 341
MslI CAYNNNNRTG 1 cut(s) 388
MspI CCGG 2 cut(s) 35, 205
MspR9I CCNGG 5 cut(s) 18, 205, 256, 298, 470
MvaI CCWGG 4 cut(s) 18, 256, 298, 470
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 103, 232, 353, 425
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 1 cut(s) 205
NcoI CCATGG 1 cut(s) 306
NdeII GATC 5 cut(s) 42, 132, 183, 324, 422
NlaIII CATG 2 cut(s) 284, 310
NlaIV GGNNCC 3 cut(s) 118, 295, 355
PagI TCATGA 1 cut(s) 280
PaqCI CACCTGC 1 cut(s) 97
PauI GCGCGC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 273
PfeI GAWTC 2 cut(s) 365, 397
PkrI GCNGC 2 cut(s) 99, 251
PluTI GGCGCC 1 cut(s) 297
PpuMI RGGWCCY 1 cut(s) 79
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 4 cut(s) 16, 254, 296, 468
PspGI CCWGG 4 cut(s) 16, 254, 296, 468
PspN4I GGNNCC 3 cut(s) 118, 295, 355
PspPI GGNCC 3 cut(s) 79, 116, 258
PspPPI RGGWCCY 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 3 cut(s) 42, 324, 422
PteI GCGCGC 1 cut(s) 235
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 388
SacI GAGCTC 1 cut(s) 60
SatI GCNGC 2 cut(s) 98, 250
Sau3AI GATC 5 cut(s) 42, 132, 183, 324, 422
Sau96I GGNCC 3 cut(s) 79, 116, 258
ScrFI CCNGG 5 cut(s) 18, 205, 256, 298, 470
SduI GDGCHC 2 cut(s) 60, 349
SetI ASST 9 cut(s) 60, 84, 111, 144, 180, 291, 417, 443, 449
SfoI GGCGCC 1 cut(s) 295
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 388
Sse9I AATT 2 cut(s) 155, 263
SsiI CCGC 2 cut(s) 98, 250
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 3 cut(s) 8, 222, 342
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 5 cut(s) 16, 203, 254, 296, 468
StyI CCWWGG 3 cut(s) 306, 341, 357
TaiI ACGT 1 cut(s) 291
TaqI TCGA 6 cut(s) 60, 84, 199, 243, 333, 386
TaqII GACCGA 1 cut(s) 420
TasI AATT 2 cut(s) 155, 263
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 100, 252
TfiI GAWTC 2 cut(s) 365, 397
TscAI CASTG 2 cut(s) 322, 468
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 2 cut(s) 322, 468
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 2 cut(s) 155, 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmaJI CCTAGG 1 cut(s) 341
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 3 cut(s) 8, 222, 342
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.