pycom11g08380

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0022434
Physical Location & Seq
Forward (+)
6506800 .. 6509857
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1218 bp
ATGGAAAGCTTCTCAAAAAGGGTTCTATTAAATGCTTTCTCCTTTCGCTCTCCTTATTATAAGGGTCATCATATACCATTACTCCCAACTACATTAGTTTCTCAGACAACAATGGCAGCATTTAATCGTGGAAAATATACCACAATTCTCAGCATTGACGGAGGAGGAGTGAGAGGCATCATCCCGGCCACCATACTTGACTGTCTTGAATCCGAACTCCAGAAACTCGATGGTGAAGATGCAAGGATTGTAGACTACTTCGACTTTATTGCCGGAACCAGCACGGGAGGCCTTATCACAGCCATGCTCACCAAACCAGATGAACAAAACAAACCTCTTTTTGCAGCAAATAAAATCGTTGAATTTTTTGTTGAAGAGGCTCCAAGATTCTTCCCTCCACCCAATCCTGCGGAGGAGCCAAGAATGGGAGAAACGGACGGCATCGTCTTGAAGACGTTTAAGAAGTGGAAGAGTAAGGCAGGAAACCTTTTGCATGAGCGTTTTTTATGGCTTATGGCACCAAAGTTCGATGGTGATAATCTGCATAACACGATCAAGCGGACCATTGGAGAAACAAAGATTCGAGATACCATAACCAACATTATCATTCCCAGTTGTGATATGAAGTACCTCCATCCGGTCGTCTTCTCCACCATGGAGGCAAAACGAGACAAGTCAAAGGATGTTCTGCTAAGTGATGTCTGCATTGCTACATCTGCAGCACCGTTCTATCTCCCTCCTCACAAATTTGAGCAGAGTTGGGAGCAGGGCACAAGAAAATATGACCTAGTCGATGGTGGTGTTGCAGCCAACAATCCCACTTTGCTTGCAATCATTGAAAGTGCCAAGGAGAAGTCTGATAATAAAAGTGGGGCAGAGCGCCTGCATATTGATAGTAGTAAGCTTCTGGTACTGTCCCTGGGAACTGGATCTGCAAAGAAGGATGCGATGCTGGAAGTCGGAGAGCCCAAAACTTGGGGAATCTGGAAATGGTTCCTGCATCCAAAAAGAAGCAGTATCCCTTTAATTGATGTTCTTACGACTCCAAGTGTCAACATGGTTGAAGTATACCTGTCTGCTTTCTTCAATGTCTCCGGCTCTGATGACAATTATCTCCGGATCCAGGAGGATGGCTTGAAACCTGATGAAATTGATATGACTGATTCAAGTCCTGAAAATCTCAAAAAACCATTTCTTTGTTCGAATAATTTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

406

Amino Acids

45.2

Weight (kDa)

6.12

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 50 - 278 8.4e-31 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022434)

Species Orthologous Gene IDs
malus_domestica MD11G1099600.v1.1
pyrus_communis pycom11g08380 pycom11g08390 pycom11g08410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 60
AasI GACNNNNNNGTC 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 517
AccB7I CCANNNNNTGG 1 cut(s) 975
AccI GTMKAC 2 cut(s) 252, 1068
AccIII TCCGGA 1 cut(s) 1116
AciI CCGC 2 cut(s) 410, 559
AclWI GGATC 3 cut(s) 937, 1114, 1127
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 362, 746
AfaI GTAC 2 cut(s) 629, 912
AfiI CCNNNNNNNGG 4 cut(s) 425, 637, 975, 1123
AgsI TTSAA 9 cut(s) 209, 362, 374, 451, 839, 1064, 1087, 1138, 1167
AjnI CCWGG 2 cut(s) 918, 1122
AluBI AGCT 2 cut(s) 9, 904
AluI AGCT 2 cut(s) 9, 904
Alw26I GTCTC 2 cut(s) 663, 1096
AlwI GGATC 3 cut(s) 937, 1114, 1127
Aor13HI TCCGGA 1 cut(s) 1116
AoxI GGCC 2 cut(s) 186, 289
ApeKI GCWGC 4 cut(s) 116, 344, 719, 806
ApoI RAATTY 2 cut(s) 362, 746
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 1 cut(s) 561
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 245, 301, 545
AsuII TTCGAA 1 cut(s) 1202
AvaII GGWCC 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 773
BamHI GGATCC 1 cut(s) 1119
BanI GGYRCC 1 cut(s) 517
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 2 cut(s) 458, 637
BbvI GCAGC 4 cut(s) 128, 356, 731, 818
BccI CCATC 5 cut(s) 224, 524, 642, 788, 1124
BceAI ACGGC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 920, 1124
BciVI GTATCC 1 cut(s) 1028
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 663, 1096
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 1 cut(s) 883
BfuI GTATCC 1 cut(s) 1028
BisI GCNGC 4 cut(s) 117, 345, 720, 807
BlsI GCNGC 4 cut(s) 118, 346, 721, 808
Bme1390I CCNGG 3 cut(s) 185, 920, 1124
Bme18I GGWCC 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 6 cut(s) 277, 381, 417, 519, 995, 1121
BmrFI CCNGG 3 cut(s) 185, 920, 1124
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 6 cut(s) 186, 229, 450, 934, 939, 1009
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 458, 637
BpmI CTGGAG 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 654, 846, 918, 919
BsaWI WCCGGW 2 cut(s) 637, 1116
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 153, 183
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 637, 975, 1123
Bse1I ACTGG 2 cut(s) 612, 931
Bse3DI GCAATG 1 cut(s) 705
BseAI TCCGGA 1 cut(s) 1116
BseBI CCWGG 2 cut(s) 920, 1124
BseDI CCNNGG 4 cut(s) 654, 846, 918, 919
BseGI GGATG 6 cut(s) 180, 634, 688, 949, 1000, 1135
BseLI CCNNNNNNNGG 4 cut(s) 425, 637, 975, 1123
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 2 cut(s) 116, 163
BseNI ACTGG 2 cut(s) 612, 931
BseRI GAGGAG 4 cut(s) 177, 180, 428, 729
BseSI GKGCMC 1 cut(s) 773
BseXI GCAGC 4 cut(s) 128, 356, 731, 818
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 2 cut(s) 188, 291
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 642
BsiSI CCGG 5 cut(s) 185, 273, 638, 1095, 1117
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 4 cut(s) 425, 637, 975, 1123
BsmAI GTCTC 2 cut(s) 663, 1096
BsmFI GGGAC 1 cut(s) 901
BsnI GGCC 2 cut(s) 188, 291
Bsp119I TTCGAA 1 cut(s) 1202
Bsp1286I GDGCHC 2 cut(s) 773, 969
Bsp13I TCCGGA 1 cut(s) 1116
Bsp143I GATC 3 cut(s) 552, 929, 1119
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 2 cut(s) 410, 559
BspANI GGCC 2 cut(s) 188, 291
BspCNI CTCAG 2 cut(s) 115, 162
BspEI TCCGGA 1 cut(s) 1116
BspLI GGNNCC 6 cut(s) 277, 381, 417, 519, 995, 1121
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 3 cut(s) 937, 1114, 1127
BspT104I TTCGAA 1 cut(s) 1202
BspT107I GGYRCC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 2 cut(s) 612, 931
BssECI CCNNGG 4 cut(s) 654, 846, 918, 919
BssMI GATC 3 cut(s) 552, 929, 1119
BssNAI GTATAC 1 cut(s) 1069
BssT1I CCWWGG 2 cut(s) 654, 846
Bst1107I GTATAC 1 cut(s) 1069
Bst2UI CCWGG 2 cut(s) 920, 1124
Bst4CI ACNGT 3 cut(s) 203, 726, 915
Bst6I CTCTTC 2 cut(s) 369, 464
BstBI TTCGAA 1 cut(s) 1202
BstC8I GCNNGC 2 cut(s) 828, 884
BstDEI CTNAG 3 cut(s) 102, 149, 692
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 6 cut(s) 180, 634, 688, 949, 1000, 1135
BstH2I RGCGCY 1 cut(s) 883
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 3 cut(s) 555, 932, 1122
BstMAI GTCTC 2 cut(s) 663, 1096
BstMBI GATC 3 cut(s) 552, 929, 1119
BstMCI CGRYCG 1 cut(s) 642
BstMWI GCNNNNNNNGC 2 cut(s) 288, 716
BstNI CCWGG 2 cut(s) 920, 1124
BstSCI CCNGG 3 cut(s) 183, 918, 1122
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 1 cut(s) 773
BstV1I GCAGC 4 cut(s) 128, 356, 731, 818
BstV2I GAAGAC 2 cut(s) 458, 637
BstX2I RGATCY 2 cut(s) 929, 1119
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 2 cut(s) 929, 1119
BstZ17I GTATAC 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 1028
BsuRI GGCC 2 cut(s) 188, 291
BtgI CCRYGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 962
BtsCI GGATG 6 cut(s) 180, 634, 688, 949, 1000, 1135
Cac8I GCNNGC 2 cut(s) 828, 884
CfoI GCGC 1 cut(s) 882
Cfr13I GGNCC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 628, 911
CspCI CAANNNNNGTGG 4 cut(s) 178, 213, 808, 843
CviAII CATG 4 cut(s) 304, 494, 655, 1057
CviQI GTAC 2 cut(s) 628, 911
DdeI CTNAG 3 cut(s) 102, 149, 692
DpnI GATC 3 cut(s) 554, 931, 1121
DpnII GATC 3 cut(s) 552, 929, 1119
DraI TTTAAA 1 cut(s) 1212
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 369, 464
EarI CTCTTC 2 cut(s) 369, 464
Eco130I CCWWGG 2 cut(s) 654, 846
Eco147I AGGCCT 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 969
Eco47I GGWCC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 918, 1122
EcoT14I CCWWGG 2 cut(s) 654, 846
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 2 cut(s) 654, 846
FaeI CATG 4 cut(s) 307, 497, 658, 1060
FaqI GGGAC 1 cut(s) 901
FatI CATG 4 cut(s) 303, 493, 654, 1056
FblI GTMKAC 2 cut(s) 252, 1068
Fnu4HI GCNGC 4 cut(s) 117, 345, 720, 807
FokI GGATG 6 cut(s) 167, 621, 695, 956, 987, 1142
FriOI GRGCYC 1 cut(s) 969
Fsp4HI GCNGC 4 cut(s) 117, 345, 720, 807
FspBI CTAG 1 cut(s) 788
GlaI GCGC 1 cut(s) 881
GluI GCNGC 4 cut(s) 117, 345, 720, 807
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 883
HaeIII GGCC 2 cut(s) 188, 291
HapII CCGG 5 cut(s) 185, 273, 638, 1095, 1117
HhaI GCGC 1 cut(s) 882
Hin1II CATG 4 cut(s) 307, 497, 658, 1060
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 7, 902
HinfI GANTC 6 cut(s) 209, 387, 580, 981, 1042, 1163
HpaII CCGG 5 cut(s) 185, 273, 638, 1095, 1117
HphI GGTGA 3 cut(s) 245, 301, 545
Hpy166II GTNNAC 3 cut(s) 253, 1054, 1069
Hpy188I TCNGA 5 cut(s) 105, 214, 859, 962, 1102
Hpy188III TCNNGA 7 cut(s) 206, 220, 448, 584, 985, 1117, 1172
Hpy8I GTNNAC 3 cut(s) 253, 1054, 1069
HpyAV CCTTC 1 cut(s) 934
HpyCH4III ACNGT 3 cut(s) 203, 726, 915
HpyCH4IV ACGT 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 716
HpyF3I CTNAG 3 cut(s) 102, 149, 692
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 4 cut(s) 307, 497, 658, 1060
HspAI GCGC 1 cut(s) 880
Kpn2I TCCGGA 1 cut(s) 1116
Kzo9I GATC 3 cut(s) 552, 929, 1119
LmnI GCTCC 3 cut(s) 385, 415, 763
Lsp1109I GCAGC 4 cut(s) 128, 356, 731, 818
LweI GCATC 6 cut(s) 186, 229, 450, 934, 939, 1009
MaeI CTAG 1 cut(s) 788
MaeII ACGT 1 cut(s) 455
MalI GATC 3 cut(s) 554, 931, 1121
MboI GATC 3 cut(s) 552, 929, 1119
MboII GAAGA 7 cut(s) 248, 382, 386, 463, 481, 637, 1075
MflI RGATCY 2 cut(s) 929, 1119
MhlI GDGCHC 2 cut(s) 773, 969
MluCI AATT 7 cut(s) 144, 362, 746, 1026, 1108, 1149, 1207
MlyI GAGTC 1 cut(s) 1036
MmeI TCCRAC 1 cut(s) 940
MroI TCCGGA 1 cut(s) 1116
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 5 cut(s) 29, 123, 459, 1025, 1211
MslI CAYNNNNRTG 1 cut(s) 302
MspI CCGG 5 cut(s) 185, 273, 638, 1095, 1117
MspR9I CCNGG 3 cut(s) 185, 920, 1124
MvaI CCWGG 2 cut(s) 920, 1124
MwoI GCNNNNNNNGC 2 cut(s) 288, 716
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 654
NdeII GATC 3 cut(s) 552, 929, 1119
NlaIII CATG 4 cut(s) 307, 497, 658, 1060
NlaIV GGNNCC 6 cut(s) 277, 381, 417, 519, 995, 1121
NspV TTCGAA 1 cut(s) 1202
PasI CCCWGGG 1 cut(s) 919
PceI AGGCCT 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 5 cut(s) 209, 387, 580, 981, 1163
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 1 cut(s) 975
PfoI TCCNGGA 1 cut(s) 1122
PkrI GCNGC 4 cut(s) 118, 346, 721, 808
PleI GAGTC 1 cut(s) 1036
PpsI GAGTC 1 cut(s) 1036
PsiI TTATAA 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 918, 1122
PspGI CCWGG 2 cut(s) 918, 1122
PspN4I GGNNCC 6 cut(s) 277, 381, 417, 519, 995, 1121
PspPI GGNCC 1 cut(s) 561
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 2 cut(s) 929, 1119
PsyI GACNNNGTC 1 cut(s) 788
RsaI GTAC 2 cut(s) 629, 912
RsaNI GTAC 2 cut(s) 628, 911
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 5 cut(s) 29, 123, 459, 1025, 1211
SatI GCNGC 4 cut(s) 117, 345, 720, 807
Sau3AI GATC 3 cut(s) 552, 929, 1119
Sau96I GGNCC 1 cut(s) 561
SchI GAGTC 1 cut(s) 1036
ScrFI CCNGG 3 cut(s) 185, 920, 1124
SduI GDGCHC 2 cut(s) 773, 969
SetI ASST 9 cut(s) 11, 337, 458, 489, 633, 789, 906, 1074, 1144
SfaNI GCATC 6 cut(s) 186, 229, 450, 934, 939, 1009
SfcI CTRYAG 1 cut(s) 717
SfuI TTCGAA 1 cut(s) 1202
SinI GGWCC 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 302
Sse9I AATT 7 cut(s) 144, 362, 746, 1026, 1108, 1149, 1207
SseBI AGGCCT 1 cut(s) 291
SsiI CCGC 2 cut(s) 410, 559
SspMI CTAG 1 cut(s) 788
StuI AGGCCT 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 183, 918, 1122
StyI CCWWGG 2 cut(s) 654, 846
TaaI ACNGT 3 cut(s) 203, 726, 915
TaiI ACGT 1 cut(s) 458
TaqI TCGA 6 cut(s) 228, 261, 528, 583, 792, 1202
TasI AATT 7 cut(s) 144, 362, 746, 1026, 1108, 1149, 1207
TfiI GAWTC 5 cut(s) 209, 387, 580, 981, 1163
Tru1I TTAA 5 cut(s) 29, 123, 459, 1025, 1211
Tru9I TTAA 5 cut(s) 29, 123, 459, 1025, 1211
TseI GCWGC 4 cut(s) 116, 344, 719, 806
TspDTI ATGAA 3 cut(s) 336, 638, 1161
TspGWI ACGGA 2 cut(s) 174, 449
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 1 cut(s) 975
VpaK11BI GGWCC 1 cut(s) 561
XapI RAATTY 2 cut(s) 362, 746
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmiI GTMKAC 2 cut(s) 252, 1068
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.