pycom11g08390

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0022434
Physical Location & Seq
Forward (+)
6513417 .. 6516579
3163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGGCAACATTTAATCGTGGAAAATATACCACAATTCTCAGCATTGACGGAGGAGGAGTGAGAGGCATCATCCCGGCCACCATACTTGACTGTCTTGAATCCGAACTCCAGAAACTCGATGGTGAAGATGCAAGGATTGTAGACTACTTCGACTTTATTGCCGGAACCAGCACGGGAGGCCTTATCACAGCCATGCTCACCAAACCAGATGAACAAAACAAACCTCTTTTTGCAGCAAATAAAATCGTTGAATTTTTTGTTGAAGAGGCTCCAAGATTCTTCCCTCCACCCAATCCTGCGGAGGAGCCAAGAATGGGAGAAACGGACGGCATCGTCTTGAAGACGTTTAAGAAGTGGAAGAGTAAGGCAGGAAACCTTTTGCATGAGCGTTTTTTATGGCTTATGGCACCAAAGTTCGATGGTGATAATCTGCATAACACGATCAAGCGGACCATTGGAGAAACAAAGATTCGAGATACCATAACCAACATTATCATTCCCAGTTGTGATATGAAGTACCTCCATCCGGTCGTCTTCTCCACCATGGAGGCAAAACGAGACAAGTCAAAGGATGTTCGGCTAAGTGATGTCTGCATTGCTACATCTGCAGCACCGTTCTATCTCCCTCCTCACAAATTTGAGCAGAGTTGGGAGCAGGGCACAAGAAAATATGACCTAGTCGACGGTGGTGTTGCAGCCAACAATCCCACTTTGCTTGCAATCATTGAAAGTGCCAAGGAGAAGTCTGATAATAAAAGTGGGGCAGAGCGCCTGCATATTGATAGTAGTAAGCTTCTGGTACTGTCCCTGGGAACTGGATCTGCAAAGAAGGATGCGATGCTGGAAGTCGGAGAGCCCAAAACTTGGGGAATCTGGAAATGGTTCCTGCATCCAAAAAGAAGCAGTATCCCTTTAATTGATGTTCTTACGACTCCAAGTGTCAACATGGTTGAAGTATACCTGTCTGCTTTCTTCAACGTCTCCGGCTCTGATGACAATTATCTCCGGATCCAGGAGGATGGCTTGAAACCTGATGAAATTGATATGACTGATTCAAGTCCTGAAAATCTCAAAAAACTTGAGAAGGCTGGGACTGACCTCCTTGAAAAAACTGTCAGTGCACTGAACCTCGACACTGGCTGGTATGTTGAACCAACTGACATGACATGCAAATACAAAGACGCAATTGCAGAATTTGCCCGAAAACTGTCCTGTGAAAAAAAGAGGCGAGCAAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

414

Amino Acids

46.12

Weight (kDa)

5.85

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 13 - 241 7.5e-31 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022434)

Species Orthologous Gene IDs
malus_domestica MD11G1099600.v1.1
pyrus_communis pycom11g08380 pycom11g08390 pycom11g08410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 406
AccB7I CCANNNNNTGG 1 cut(s) 864
AccI GTMKAC 3 cut(s) 141, 681, 957
AccIII TCCGGA 1 cut(s) 1005
AciI CCGC 2 cut(s) 299, 448
AclWI GGATC 3 cut(s) 826, 1003, 1016
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 251, 635, 1193
AfaI GTAC 2 cut(s) 518, 801
AfiI CCNNNNNNNGG 4 cut(s) 314, 526, 864, 1012
AjnI CCWGG 2 cut(s) 807, 1011
AluBI AGCT 1 cut(s) 793
AluI AGCT 1 cut(s) 793
Alw21I GWGCWC 1 cut(s) 1123
Alw26I GTCTC 2 cut(s) 552, 985
Alw44I GTGCAC 1 cut(s) 1119
AlwI GGATC 3 cut(s) 826, 1003, 1016
Aor13HI TCCGGA 1 cut(s) 1005
AoxI GGCC 2 cut(s) 75, 178
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 3 cut(s) 233, 608, 695
ApoI RAATTY 3 cut(s) 251, 635, 1193
Asp700I GAANNNNTTC 1 cut(s) 881
AspLEI GCGC 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 134, 190, 434
AvaII GGWCC 1 cut(s) 450
BaeGI GKGCMC 2 cut(s) 662, 1123
BamHI GGATCC 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 858
BbsI GAAGAC 2 cut(s) 347, 526
Bbv12I GWGCWC 1 cut(s) 1123
BbvI GCAGC 3 cut(s) 245, 620, 707
BccI CCATC 4 cut(s) 113, 413, 531, 1013
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 2 cut(s) 809, 1013
BciVI GTATCC 1 cut(s) 917
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 2 cut(s) 552, 985
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 772
BfuI GTATCC 1 cut(s) 917
BisI GCNGC 3 cut(s) 234, 609, 696
BlsI GCNGC 3 cut(s) 235, 610, 697
Bme1390I CCNGG 3 cut(s) 74, 809, 1013
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 6 cut(s) 166, 270, 306, 408, 884, 1010
BmrFI CCNGG 3 cut(s) 74, 809, 1013
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 6 cut(s) 75, 118, 339, 823, 828, 898
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 347, 526
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 1100
BpuMI CCSGG 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 543, 735, 807, 808
BsaWI WCCGGW 2 cut(s) 526, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 526, 864, 1012
Bse1I ACTGG 3 cut(s) 501, 820, 1141
Bse3DI GCAATG 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 809, 1013
BseDI CCNNGG 4 cut(s) 543, 735, 807, 808
BseGI GGATG 6 cut(s) 69, 523, 577, 838, 889, 1024
BseLI CCNNNNNNNGG 4 cut(s) 314, 526, 864, 1012
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 3 cut(s) 501, 820, 1141
BseRI GAGGAG 4 cut(s) 66, 69, 317, 618
BseSI GKGCMC 2 cut(s) 662, 1123
BseXI GCAGC 3 cut(s) 245, 620, 707
BseYI CCCAGC 1 cut(s) 1088
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 2 cut(s) 77, 180
BshNI GGYRCC 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 1123
BsiSI CCGG 5 cut(s) 74, 162, 527, 984, 1006
BslFI GGGAC 2 cut(s) 790, 1105
BslI CCNNNNNNNGG 4 cut(s) 314, 526, 864, 1012
BsmAI GTCTC 2 cut(s) 552, 985
BsmBI CGTCTC 1 cut(s) 985
BsmFI GGGAC 2 cut(s) 790, 1105
BsnI GGCC 2 cut(s) 77, 180
Bsp1286I GDGCHC 3 cut(s) 662, 858, 1123
Bsp13I TCCGGA 1 cut(s) 1005
Bsp143I GATC 3 cut(s) 441, 818, 1008
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 2 cut(s) 299, 448
BspANI GGCC 2 cut(s) 77, 180
BspCNI CTCAG 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 1005
BspLI GGNNCC 6 cut(s) 166, 270, 306, 408, 884, 1010
BspMAI CTGCAG 1 cut(s) 610
BspPI GGATC 3 cut(s) 826, 1003, 1016
BspT107I GGYRCC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 3 cut(s) 501, 820, 1141
BssECI CCNNGG 4 cut(s) 543, 735, 807, 808
BssMI GATC 3 cut(s) 441, 818, 1008
BssNAI GTATAC 1 cut(s) 958
BssT1I CCWWGG 2 cut(s) 543, 735
Bst1107I GTATAC 1 cut(s) 958
Bst2UI CCWGG 2 cut(s) 809, 1013
Bst4CI ACNGT 6 cut(s) 92, 615, 686, 804, 1114, 1209
Bst6I CTCTTC 2 cut(s) 258, 353
BstAPI GCANNNNNTGC 1 cut(s) 1196
BstC8I GCNNGC 3 cut(s) 717, 773, 1230
BstDEI CTNAG 2 cut(s) 38, 581
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 6 cut(s) 69, 523, 577, 838, 889, 1024
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 1 cut(s) 771
BstKTI GATC 3 cut(s) 444, 821, 1011
BstMAI GTCTC 2 cut(s) 552, 985
BstMBI GATC 3 cut(s) 441, 818, 1008
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 3 cut(s) 177, 605, 1196
BstNI CCWGG 2 cut(s) 809, 1013
BstNSI RCATGY 1 cut(s) 1170
BstSCI CCNGG 3 cut(s) 72, 807, 1011
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 2 cut(s) 662, 1123
BstV1I GCAGC 3 cut(s) 245, 620, 707
BstV2I GAAGAC 2 cut(s) 347, 526
BstX2I RGATCY 2 cut(s) 818, 1008
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 2 cut(s) 818, 1008
BstZ17I GTATAC 1 cut(s) 958
BsuI GTATCC 1 cut(s) 917
BsuRI GGCC 2 cut(s) 77, 180
BtgI CCRYGG 1 cut(s) 543
BtgZI GCGATG 1 cut(s) 851
BtsCI GGATG 6 cut(s) 69, 523, 577, 838, 889, 1024
BtsIMutI CAGTG 3 cut(s) 1121, 1123, 1134
Cac8I GCNNGC 3 cut(s) 717, 773, 1230
CfoI GCGC 1 cut(s) 771
Cfr13I GGNCC 1 cut(s) 450
CseI GACGC 1 cut(s) 1190
Csp6I GTAC 2 cut(s) 517, 800
CspCI CAANNNNNGTGG 4 cut(s) 67, 102, 697, 732
CviAII CATG 7 cut(s) 193, 383, 544, 946, 1162, 1167, 1239
CviQI GTAC 2 cut(s) 517, 800
DdeI CTNAG 2 cut(s) 38, 581
DpnI GATC 3 cut(s) 443, 820, 1010
DpnII GATC 3 cut(s) 441, 818, 1008
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 258, 353
EarI CTCTTC 2 cut(s) 258, 353
Eco130I CCWWGG 2 cut(s) 543, 735
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 858
Eco47I GGWCC 1 cut(s) 450
EcoRII CCWGG 2 cut(s) 807, 1011
EcoT14I CCWWGG 2 cut(s) 543, 735
EcoT38I GRGCYC 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 543, 735
Esp3I CGTCTC 1 cut(s) 985
FaeI CATG 7 cut(s) 196, 386, 547, 949, 1165, 1170, 1242
FaqI GGGAC 2 cut(s) 790, 1105
FatI CATG 7 cut(s) 192, 382, 543, 945, 1161, 1166, 1238
FblI GTMKAC 3 cut(s) 141, 681, 957
Fnu4HI GCNGC 3 cut(s) 234, 609, 696
FokI GGATG 6 cut(s) 56, 510, 584, 845, 876, 1031
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 3 cut(s) 234, 609, 696
FspBI CTAG 1 cut(s) 677
GlaI GCGC 1 cut(s) 770
GluI GCNGC 3 cut(s) 234, 609, 696
GsaI CCCAGC 1 cut(s) 1092
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 2 cut(s) 77, 180
HapII CCGG 5 cut(s) 74, 162, 527, 984, 1006
HgaI GACGC 1 cut(s) 1190
HhaI GCGC 1 cut(s) 771
Hin1II CATG 7 cut(s) 196, 386, 547, 949, 1165, 1170, 1242
Hin6I GCGC 1 cut(s) 769
HinP1I GCGC 1 cut(s) 769
HincII GTYRAC 2 cut(s) 682, 943
HindII GTYRAC 2 cut(s) 682, 943
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 6 cut(s) 98, 276, 469, 870, 931, 1052
HpaII CCGG 5 cut(s) 74, 162, 527, 984, 1006
HphI GGTGA 3 cut(s) 134, 190, 434
Hpy166II GTNNAC 5 cut(s) 142, 682, 943, 958, 1121
Hpy188I TCNGA 4 cut(s) 103, 748, 851, 991
Hpy188III TCNNGA 7 cut(s) 95, 109, 337, 473, 874, 1006, 1061
Hpy8I GTNNAC 5 cut(s) 142, 682, 943, 958, 1121
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 2 cut(s) 823, 1078
HpyCH4III ACNGT 6 cut(s) 92, 615, 686, 804, 1114, 1209
HpyCH4IV ACGT 2 cut(s) 344, 978
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 605, 1196
HpyF3I CTNAG 2 cut(s) 38, 581
HpySE526I ACGT 2 cut(s) 344, 978
Hsp92II CATG 7 cut(s) 196, 386, 547, 949, 1165, 1170, 1242
HspAI GCGC 1 cut(s) 769
Kpn2I TCCGGA 1 cut(s) 1005
Kzo9I GATC 3 cut(s) 441, 818, 1008
LmnI GCTCC 3 cut(s) 274, 304, 652
Lsp1109I GCAGC 3 cut(s) 245, 620, 707
LweI GCATC 6 cut(s) 75, 118, 339, 823, 828, 898
MaeI CTAG 1 cut(s) 677
MaeII ACGT 2 cut(s) 344, 978
MalI GATC 3 cut(s) 443, 820, 1010
MboI GATC 3 cut(s) 441, 818, 1008
MboII GAAGA 7 cut(s) 137, 271, 275, 352, 370, 526, 964
MfeI CAATTG 1 cut(s) 1185
MflI RGATCY 2 cut(s) 818, 1008
MhlI GDGCHC 3 cut(s) 662, 858, 1123
MluCI AATT 8 cut(s) 33, 251, 635, 915, 997, 1038, 1185, 1193
MlyI GAGTC 1 cut(s) 925
MmeI TCCRAC 1 cut(s) 829
MroI TCCGGA 1 cut(s) 1005
MroXI GAANNNNTTC 1 cut(s) 881
MseI TTAA 3 cut(s) 12, 348, 914
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 5 cut(s) 74, 162, 527, 984, 1006
MspR9I CCNGG 3 cut(s) 74, 809, 1013
MunI CAATTG 1 cut(s) 1185
MvaI CCWGG 2 cut(s) 809, 1013
MwoI GCNNNNNNNGC 3 cut(s) 177, 605, 1196
NciI CCSGG 1 cut(s) 74
NcoI CCATGG 1 cut(s) 543
NdeII GATC 3 cut(s) 441, 818, 1008
NlaIII CATG 7 cut(s) 196, 386, 547, 949, 1165, 1170, 1242
NlaIV GGNNCC 6 cut(s) 166, 270, 306, 408, 884, 1010
NspI RCATGY 1 cut(s) 1170
PasI CCCWGGG 1 cut(s) 808
PceI AGGCCT 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 5 cut(s) 98, 276, 469, 870, 1052
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 1 cut(s) 864
PfoI TCCNGGA 1 cut(s) 1011
PkrI GCNGC 3 cut(s) 235, 610, 697
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
Psp6I CCWGG 2 cut(s) 807, 1011
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 807, 1011
PspN4I GGNNCC 6 cut(s) 166, 270, 306, 408, 884, 1010
PspPI GGNCC 1 cut(s) 450
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 2 cut(s) 818, 1008
PsyI GACNNNGTC 1 cut(s) 677
RsaI GTAC 2 cut(s) 518, 801
RsaNI GTAC 2 cut(s) 517, 800
RseI CAYNNNNRTG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 12, 348, 914
SatI GCNGC 3 cut(s) 234, 609, 696
Sau3AI GATC 3 cut(s) 441, 818, 1008
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 1 cut(s) 925
ScrFI CCNGG 3 cut(s) 74, 809, 1013
SduI GDGCHC 3 cut(s) 662, 858, 1123
SfaNI GCATC 6 cut(s) 75, 118, 339, 823, 828, 898
SfcI CTRYAG 1 cut(s) 606
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
Sse9I AATT 8 cut(s) 33, 251, 635, 915, 997, 1038, 1185, 1193
SseBI AGGCCT 1 cut(s) 180
SsiI CCGC 2 cut(s) 299, 448
SspMI CTAG 1 cut(s) 677
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 72, 807, 1011
StyI CCWWGG 2 cut(s) 543, 735
TaaI ACNGT 6 cut(s) 92, 615, 686, 804, 1114, 1209
TaiI ACGT 2 cut(s) 347, 981
TaqI TCGA 6 cut(s) 117, 150, 417, 472, 681, 1131
TasI AATT 8 cut(s) 33, 251, 635, 915, 997, 1038, 1185, 1193
TfiI GAWTC 5 cut(s) 98, 276, 469, 870, 1052
Tru1I TTAA 3 cut(s) 12, 348, 914
Tru9I TTAA 3 cut(s) 12, 348, 914
TscAI CASTG 3 cut(s) 1123, 1128, 1141
TseI GCWGC 3 cut(s) 233, 608, 695
TspDTI ATGAA 3 cut(s) 225, 527, 1050
TspGWI ACGGA 2 cut(s) 63, 338
TspRI CASTG 3 cut(s) 1123, 1128, 1141
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 864
VneI GTGCAC 1 cut(s) 1119
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 3 cut(s) 251, 635, 1193
XceI RCATGY 1 cut(s) 1170
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XmiI GTMKAC 3 cut(s) 141, 681, 957
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.