Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0020959

Physical Location & Seq

Genomic Coordinates Reverse (-)

20331934 .. 20333508

Gene Structure 1575 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGTTGAATGTAAACCAATTACTTGCAAATTGCAAAACCCTCAACCATCTAAAGCAACTGCACGTCTTTATCATCACTCTTGGTTACGGCCAGAGTGAAATCTACGCCTTCAAGCTCGTCCGCTTCTGCCTTGTCACTCTCCCCAATGCCAACCTTCCCTATGCTCGCCTCATCTTCGACTGCCTCCACTACCCGAATGTATACCATTACGCTGCCATGATTGCCGGTTATGCCTCCCAGTCCCACCATCATTCTGTTTTTTTGTTGTACCGACGTATGTTTTGTCAGGGAAGCCCTCAACCCAACCATTTTATCTACCCCCACATCTTGAAATCTAGTCCAGAGGTTTTTAAGTCTCACGGGACTGAATTAGTGCATACCCACATCATGAAATCGGGTTTTGGCCAATACCCTGTTGTGCAAACAGCTCTTGTTGATTCCTACTCGAGGTTCCGCTCCGATGTTGTCTCAGCAAGGCGGGTGTTTGATGAAATGTCTGAGAAAAATGTCGTGACCTGGACGGCTATGATTTCCGGGTACACTAGAGTTGGGGACATTGGTGGTGCTCTGTTGTTGTTCGAGAAAATGCCAGAGAGAGATGTCCCTTCTTGGAATGCTGTTATTGCGGGGTGCACGCAGAATGGCCAGTTCTCGGAGGCAATTTCTCTGTTCAAAACAATGGTTCATATTGCACATGGGGAGAAACAGCGAGAAAATAGGCCGAATCAGGTTACTGCTGTATGTGTTCTTTCGGCTTGTAGTCACACTGGCATGCTGCAGCTTGGTAAATCTATGCACAACTATGTTTACAAAAATGCGCTTGGTCCAGATTCATTTGTTTCCAATGCTTTAGTAGATATGTATGGGAAATGTGGGAGCTTGAAAGTGGCAAGAAGGGTTTTCGACAGGACCTCGCAAAAAAGCTTGACCTCTTGGAATTCCATGATAAACTCTTATGCTCTGCACGGCGATAGTGACAATGCAATTGGTGTGTTTGAGGACATGATACAGTGTGGGGCTGATGTAAGACCAGATGAGGTCACTTTTGTAGGCTTATTCAATGCTTGCACGCATGGAGGGTTGGTAGAGAAAGGTATTTCTTATTTTGATTTGATGACTCTGGGTTATGGGATTGAGCCTCAGATAGAACACTATGGATGCTTGATAGACCTTCTTGGTCGAGCAGGTCGATTTGAGGAAGCCATGGAAGCTGTGAGGGGAATGAAAATTGAACCCGATGAGGTCATATGGGGTTCTCTGCTTAATGGATGTAAGATTTATGGTCGCACAGATCTCGCAGAAATTGCAGTTAAAAGGTTGATTCAACTTGAACCAAATAATGGTGCGTATGGGACCATGTTGGCCAATATATATGGGCAGTTGGGGAAGTGGGATGATGTCCGAAATGTGAGAAAGATGCTGAGGGAGCGAAATGCTTATAAAACCCCAGGTTGCAGTTGGATTGAGGTTGATAATCAAGTTTACCAATTTTATTCAATTGACAAATCACATCCTAGAACGGAAGAGATCTACCAAATCCTAGACATTCTCCTCTTATTTCTTCCCGACATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

525

Amino Acids

59.1

Weight (kDa)

7.03

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	168 - 201	1.6e-06	PPR repeat family
PPR	PF01535	171 - 200	4.3e-07	PPR repeat
PPR_2	PF13041	200 - 254	7.8e-06	PPR repeat family
PPR	PF01535	203 - 228	4.9e-06	PPR repeat
PPR_2	PF13041	310 - 357	8.2e-08	PPR repeat family
PPR	PF01535	311 - 339	2.9e-07	PPR repeat
E_motif	PF20431	430 - 489	3.5e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33350 AT1G33350
malus_domestica	MD11G1207700.v1.1
prunus_persica	Prupe.8G000500_v2.0.a1
pyrus_communis	pycom11g18090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1440
Acc36I	ACCTGC	1 cut(s)	1175
AccB7I	CCANNNNNTGG	1 cut(s)	1340
AccBSI	CCGCTC	1 cut(s)	456
AccI	GTMKAC	1 cut(s)	201
AciI	CCGC	4 cut(s)	121, 454, 478, 626
AcoI	YGGCCR	4 cut(s)	88, 403, 643, 1362
AcsI	RAATTY	1 cut(s)	937
AfaI	GTAC	2 cut(s)	269, 539
AfiI	CCNNNNNNNGG	3 cut(s)	447, 652, 1340
AjiI	CACGTC	1 cut(s)	64
AjnI	CCWGG	2 cut(s)	515, 1447
AluBI	AGCT	6 cut(s)	115, 428, 781, 879, 924, 1211
AluI	AGCT	6 cut(s)	115, 428, 781, 879, 924, 1211
Alw21I	GWGCWC	2 cut(s)	568, 635
Alw26I	GTCTC	2 cut(s)	360, 472
Alw44I	GTGCAC	1 cut(s)	631
Ama87I	CYCGRG	1 cut(s)	445
AoxI	GGCC	5 cut(s)	88, 403, 643, 719, 1362
ApaLI	GTGCAC	1 cut(s)	631
ApeKI	GCWGC	3 cut(s)	212, 775, 778
ApoI	RAATTY	1 cut(s)	937
ArsI	GACNNNNNNTTYG	2 cut(s)	264, 296
AspLEI	GCGC	1 cut(s)	820
AspS9I	GGNCC	3 cut(s)	824, 909, 1353
AsuC2I	CCSGG	1 cut(s)	535
AvaI	CYCGRG	1 cut(s)	445
AvaII	GGWCC	3 cut(s)	824, 909, 1353
BaeGI	GKGCMC	1 cut(s)	635
BalI	TGGCCA	3 cut(s)	405, 645, 1364
Bbv12I	GWGCWC	2 cut(s)	568, 635
BbvCI	CCTCAGC	1 cut(s)	1421
BbvI	GCAGC	3 cut(s)	199, 762, 790
BccI	CCATC	2 cut(s)	54, 255
BceAI	ACGGC	3 cut(s)	103, 537, 982
BcgI	CGANNNNNNTGC	2 cut(s)	1276, 1310
BciT130I	CCWGG	2 cut(s)	517, 1449
BcnI	CCSGG	1 cut(s)	535
BcoDI	GTCTC	2 cut(s)	360, 472
BfaI	CTAG	4 cut(s)	336, 543, 1515, 1541
BfmI	CTRYAG	1 cut(s)	776
BfuAI	ACCTGC	1 cut(s)	1175
BglII	AGATCT	2 cut(s)	1291, 1527
BisI	GCNGC	3 cut(s)	213, 776, 779
BlsI	GCNGC	3 cut(s)	214, 777, 780
Bme1390I	CCNGG	3 cut(s)	517, 535, 1449
Bme18I	GGWCC	3 cut(s)	824, 909, 1353
BmeT110I	CYCGRG	1 cut(s)	445
BmgBI	CACGTC	1 cut(s)	64
BmgT120I	GGNCC	3 cut(s)	824, 909, 1353
BmiI	GGNNCC	2 cut(s)	452, 1354
BmrFI	CCNGG	3 cut(s)	517, 535, 1449
BmrI	ACTGGG	1 cut(s)	232
BmsI	GCATC	2 cut(s)	1148, 1407
BmuI	ACTGGG	1 cut(s)	232
Bpu10I	CCTNAGC	1 cut(s)	1421
BpuMI	CCSGG	1 cut(s)	535
BsaJI	CCNNGG	2 cut(s)	1203, 1447
BsaXI	ACNNNNNCTCC	2 cut(s)	1068, 1098
Bsc4I	CCNNNNNNNGG	3 cut(s)	447, 652, 1340
Bse118I	RCCGGY	1 cut(s)	224
Bse1I	ACTGG	3 cut(s)	238, 646, 772
BseBI	CCWGG	2 cut(s)	517, 1449
BseDI	CCNNGG	2 cut(s)	1203, 1447
BseGI	GGATG	4 cut(s)	1163, 1274, 1399, 1510
BseLI	CCNNNNNNNGG	3 cut(s)	447, 652, 1340
BseMII	CTCAG	4 cut(s)	483, 489, 1154, 1412
BseNI	ACTGG	3 cut(s)	238, 646, 772
BseRI	GAGGAG	1 cut(s)	1541
BseSI	GKGCMC	1 cut(s)	635
BseXI	GCAGC	3 cut(s)	199, 762, 790
BsgI	GTGCAG	2 cut(s)	44, 947
BshFI	GGCC	5 cut(s)	90, 405, 645, 721, 1364
BsiHKAI	GWGCWC	2 cut(s)	568, 635
BsiHKCI	CYCGRG	1 cut(s)	445
BsiSI	CCGG	2 cut(s)	225, 534
BslFI	GGGAC	5 cut(s)	226, 376, 566, 587, 1366
BslI	CCNNNNNNNGG	3 cut(s)	447, 652, 1340
BsmAI	GTCTC	2 cut(s)	360, 472
BsmFI	GGGAC	5 cut(s)	226, 376, 566, 587, 1366
BsmI	GAATGC	1 cut(s)	619
BsnI	GGCC	5 cut(s)	90, 405, 645, 721, 1364
BsoBI	CYCGRG	1 cut(s)	445
Bsp1286I	GDGCHC	2 cut(s)	568, 635
Bsp143I	GATC	2 cut(s)	1291, 1527
Bsp19I	CCATGG	1 cut(s)	1203
BspACI	CCGC	4 cut(s)	121, 454, 478, 626
BspANI	GGCC	5 cut(s)	90, 405, 645, 721, 1364
BspCNI	CTCAG	4 cut(s)	482, 490, 1153, 1413
BspHI	TCATGA	1 cut(s)	387
BspLI	GGNNCC	2 cut(s)	452, 1354
BspMAI	CTGCAG	1 cut(s)	780
BspMI	ACCTGC	1 cut(s)	1175
BsrBI	CCGCTC	1 cut(s)	456
BsrFI	RCCGGY	1 cut(s)	224
BsrI	ACTGG	3 cut(s)	238, 646, 772
BssAI	RCCGGY	1 cut(s)	224
BssECI	CCNNGG	2 cut(s)	1203, 1447
BssMI	GATC	2 cut(s)	1291, 1527
BssNAI	GTATAC	1 cut(s)	202
BssT1I	CCWWGG	1 cut(s)	1203
Bst1107I	GTATAC	1 cut(s)	202
Bst2UI	CCWGG	2 cut(s)	517, 1449
Bst4CI	ACNGT	1 cut(s)	1011
Bst6I	CTCTTC	1 cut(s)	1518
BstAPI	GCANNNNNTGC	1 cut(s)	1304
BstC8I	GCNNGC	5 cut(s)	166, 635, 773, 1066, 1070
BstDEI	CTNAG	4 cut(s)	469, 498, 1140, 1421
BstDSI	CCRYGG	1 cut(s)	1203
BstENI	CCTNNNNNAGG	1 cut(s)	445
BstF5I	GGATG	4 cut(s)	1163, 1274, 1399, 1510
BstHHI	GCGC	1 cut(s)	820
BstKTI	GATC	2 cut(s)	1294, 1530
BstMAI	GTCTC	2 cut(s)	360, 472
BstMBI	GATC	2 cut(s)	1291, 1527
BstMWI	GCNNNNNNNGC	6 cut(s)	221, 230, 623, 1208, 1304, 1426
BstNI	CCWGG	2 cut(s)	517, 1449
BstNSI	RCATGY	1 cut(s)	775
BstSCI	CCNGG	3 cut(s)	515, 533, 1447
BstSFI	CTRYAG	1 cut(s)	776
BstSLI	GKGCMC	1 cut(s)	635
BstV1I	GCAGC	3 cut(s)	199, 762, 790
BstX2I	RGATCY	2 cut(s)	1291, 1527
BstYI	RGATCY	2 cut(s)	1291, 1527
BstZ17I	GTATAC	1 cut(s)	202
BsuRI	GGCC	5 cut(s)	90, 405, 645, 721, 1364
BtgI	CCRYGG	1 cut(s)	1203
BtrI	CACGTC	1 cut(s)	64
BtsCI	GGATG	4 cut(s)	1163, 1274, 1399, 1510
BtsIMutI	CAGTG	2 cut(s)	765, 1016
BveI	ACCTGC	1 cut(s)	1175
Cac8I	GCNNGC	5 cut(s)	166, 635, 773, 1066, 1070
CciI	TCATGA	1 cut(s)	387
CfoI	GCGC	1 cut(s)	820
Cfr10I	RCCGGY	1 cut(s)	224
Cfr13I	GGNCC	3 cut(s)	824, 909, 1353
Csp6I	GTAC	2 cut(s)	268, 538
CviAII	CATG	9 cut(s)	217, 388, 695, 772, 943, 1003, 1073, 1204, 1357
CviQI	GTAC	2 cut(s)	268, 538
DdeI	CTNAG	4 cut(s)	469, 498, 1140, 1421
DpnI	GATC	2 cut(s)	1293, 1529
DpnII	GATC	2 cut(s)	1291, 1527
EaeI	YGGCCR	4 cut(s)	88, 403, 643, 1362
Eam1104I	CTCTTC	1 cut(s)	1518
EarI	CTCTTC	1 cut(s)	1518
Eco130I	CCWWGG	1 cut(s)	1203
Eco47I	GGWCC	3 cut(s)	824, 909, 1353
Eco88I	CYCGRG	1 cut(s)	445
EcoNI	CCTNNNNNAGG	1 cut(s)	445
EcoO109I	RGGNCCY	1 cut(s)	909
EcoRI	GAATTC	1 cut(s)	937
EcoRII	CCWGG	2 cut(s)	515, 1447
EcoT14I	CCWWGG	1 cut(s)	1203
ErhI	CCWWGG	1 cut(s)	1203
FaeI	CATG	9 cut(s)	220, 391, 698, 775, 946, 1006, 1076, 1207, 1360
FaqI	GGGAC	5 cut(s)	226, 376, 566, 587, 1366
FatI	CATG	9 cut(s)	216, 387, 694, 771, 942, 1002, 1072, 1203, 1356
FauI	CCCGC	2 cut(s)	471, 619
FauNDI	CATATG	2 cut(s)	1247, 1571
FblI	GTMKAC	1 cut(s)	201
Fnu4HI	GCNGC	3 cut(s)	213, 776, 779
FokI	GGATG	4 cut(s)	1170, 1281, 1406, 1497
Fsp4HI	GCNGC	3 cut(s)	213, 776, 779
FspBI	CTAG	4 cut(s)	336, 543, 1515, 1541
GlaI	GCGC	1 cut(s)	819
GluI	GCNGC	3 cut(s)	213, 776, 779
HaeIII	GGCC	5 cut(s)	90, 405, 645, 721, 1364
HapII	CCGG	2 cut(s)	225, 534
HhaI	GCGC	1 cut(s)	820
Hin1II	CATG	9 cut(s)	220, 391, 698, 775, 946, 1006, 1076, 1207, 1360
Hin6I	GCGC	1 cut(s)	818
HinP1I	GCGC	1 cut(s)	818
HindIII	AAGCTT	1 cut(s)	922
HinfI	GANTC	5 cut(s)	437, 724, 830, 1117, 1321
HpaII	CCGG	2 cut(s)	225, 534
Hpy166II	GTNNAC	6 cut(s)	13, 202, 540, 633, 808, 1483
Hpy188I	TCNGA	5 cut(s)	460, 499, 655, 1143, 1403
Hpy188III	TCNNGA	7 cut(s)	328, 341, 388, 511, 580, 827, 1565
Hpy8I	GTNNAC	6 cut(s)	13, 202, 540, 633, 808, 1483
Hpy99I	CGWCG	1 cut(s)	276
HpyAV	CCTTC	5 cut(s)	118, 164, 615, 888, 1181
HpyCH4III	ACNGT	1 cut(s)	1011
HpyCH4IV	ACGT	2 cut(s)	63, 274
HpyF10VI	GCNNNNNNNGC	6 cut(s)	221, 230, 623, 1208, 1304, 1426
HpyF3I	CTNAG	4 cut(s)	469, 498, 1140, 1421
HpySE526I	ACGT	2 cut(s)	63, 274
Hsp92II	CATG	9 cut(s)	220, 391, 698, 775, 946, 1006, 1076, 1207, 1360
HspAI	GCGC	1 cut(s)	818
Kzo9I	GATC	2 cut(s)	1291, 1527
LmnI	GCTCC	3 cut(s)	461, 876, 1426
Lsp1109I	GCAGC	3 cut(s)	199, 762, 790
LweI	GCATC	2 cut(s)	1148, 1407
MaeI	CTAG	4 cut(s)	336, 543, 1515, 1541
MaeII	ACGT	2 cut(s)	63, 274
MaeIII	GTNAC	7 cut(s)	83, 133, 511, 730, 761, 974, 1039
MalI	GATC	2 cut(s)	1293, 1529
MbiI	CCGCTC	1 cut(s)	456
MboI	GATC	2 cut(s)	1291, 1527
MboII	GAAGA	3 cut(s)	166, 1535, 1553
MfeI	CAATTG	2 cut(s)	984, 1497
MflI	RGATCY	2 cut(s)	1291, 1527
MhlI	GDGCHC	2 cut(s)	568, 635
MlsI	TGGCCA	3 cut(s)	405, 645, 1364
MluNI	TGGCCA	3 cut(s)	405, 645, 1364
MlyI	GAGTC	1 cut(s)	1111
MmeI	TCCRAC	1 cut(s)	1439
Mox20I	TGGCCA	3 cut(s)	405, 645, 1364
MscI	TGGCCA	3 cut(s)	405, 645, 1364
MseI	TTAA	3 cut(s)	351, 1263, 1311
MslI	CAYNNNNRTG	2 cut(s)	770, 801
Msp20I	TGGCCA	3 cut(s)	405, 645, 1364
MspI	CCGG	2 cut(s)	225, 534
MspR9I	CCNGG	3 cut(s)	517, 535, 1449
MunI	CAATTG	2 cut(s)	984, 1497
Mva1269I	GAATGC	1 cut(s)	619
MvaI	CCWGG	2 cut(s)	517, 1449
MwoI	GCNNNNNNNGC	6 cut(s)	221, 230, 623, 1208, 1304, 1426
NciI	CCSGG	1 cut(s)	535
NcoI	CCATGG	1 cut(s)	1203
NdeI	CATATG	2 cut(s)	1247, 1571
NdeII	GATC	2 cut(s)	1291, 1527
NlaIII	CATG	9 cut(s)	220, 391, 698, 775, 946, 1006, 1076, 1207, 1360
NlaIV	GGNNCC	2 cut(s)	452, 1354
NmuCI	GTSAC	5 cut(s)	133, 511, 761, 974, 1039
NspI	RCATGY	1 cut(s)	775
PaeI	GCATGC	1 cut(s)	775
PaeR7I	CTCGAG	1 cut(s)	445
PagI	TCATGA	1 cut(s)	387
PcsI	WCGNNNNNNNCGW	1 cut(s)	1186
PctI	GAATGC	1 cut(s)	619
PfeI	GAWTC	4 cut(s)	437, 724, 830, 1321
PflMI	CCANNNNNTGG	1 cut(s)	1340
PkrI	GCNGC	3 cut(s)	214, 777, 780
PleI	GAGTC	1 cut(s)	1111
PpsI	GAGTC	1 cut(s)	1111
PpuMI	RGGWCCY	1 cut(s)	909
PsiI	TTATAA	1 cut(s)	1440
Psp5II	RGGWCCY	1 cut(s)	909
Psp6I	CCWGG	2 cut(s)	515, 1447
PspGI	CCWGG	2 cut(s)	515, 1447
PspN4I	GGNNCC	2 cut(s)	452, 1354
PspPI	GGNCC	3 cut(s)	824, 909, 1353
PspPPI	RGGWCCY	1 cut(s)	909
PspXI	VCTCGAGB	1 cut(s)	445
PstI	CTGCAG	1 cut(s)	780
PsuI	RGATCY	2 cut(s)	1291, 1527
RsaI	GTAC	2 cut(s)	269, 539
RsaNI	GTAC	2 cut(s)	268, 538
RseI	CAYNNNNRTG	2 cut(s)	770, 801
SaqAI	TTAA	3 cut(s)	351, 1263, 1311
SatI	GCNGC	3 cut(s)	213, 776, 779
Sau3AI	GATC	2 cut(s)	1291, 1527
Sau96I	GGNCC	3 cut(s)	824, 909, 1353
SchI	GAGTC	1 cut(s)	1111
ScrFI	CCNGG	3 cut(s)	517, 535, 1449
SduI	GDGCHC	2 cut(s)	568, 635
SfaNI	GCATC	2 cut(s)	1148, 1407
SfcI	CTRYAG	1 cut(s)	776
Sfr274I	CTCGAG	1 cut(s)	445
SinI	GGWCC	3 cut(s)	824, 909, 1353
SlaI	CTCGAG	1 cut(s)	445
SmiMI	CAYNNNNRTG	2 cut(s)	770, 801
SmlI	CTYRAG	1 cut(s)	445
SmoI	CTYRAG	1 cut(s)	445
SphI	GCATGC	1 cut(s)	775
SsiI	CCGC	4 cut(s)	121, 454, 478, 626
SspMI	CTAG	4 cut(s)	336, 543, 1515, 1541
StyD4I	CCNGG	3 cut(s)	515, 533, 1447
StyI	CCWWGG	1 cut(s)	1203
TaaI	ACNGT	1 cut(s)	1011
TaiI	ACGT	2 cut(s)	66, 277
TaqI	TCGA	6 cut(s)	177, 446, 579, 903, 1180, 1189
TfiI	GAWTC	4 cut(s)	437, 724, 830, 1321
Tru1I	TTAA	3 cut(s)	351, 1263, 1311
Tru9I	TTAA	3 cut(s)	351, 1263, 1311
TscAI	CASTG	2 cut(s)	772, 1016
TseFI	GTSAC	5 cut(s)	133, 511, 761, 974, 1039
TseI	GCWGC	3 cut(s)	212, 775, 778
Tsp45I	GTSAC	5 cut(s)	133, 511, 761, 974, 1039
TspDTI	ATGAA	5 cut(s)	404, 504, 674, 822, 1238
TspGWI	ACGGA	1 cut(s)	1535
TspRI	CASTG	2 cut(s)	772, 1016
Van91I	CCANNNNNTGG	1 cut(s)	1340
VneI	GTGCAC	1 cut(s)	631
VpaK11BI	GGWCC	3 cut(s)	824, 909, 1353
XagI	CCTNNNNNAGG	1 cut(s)	445
XapI	RAATTY	1 cut(s)	937
XceI	RCATGY	1 cut(s)	775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1455
XhoI	CTCGAG	1 cut(s)	445
XmiI	GTMKAC	1 cut(s)	201
XspI	CTAG	4 cut(s)	336, 543, 1515, 1541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.