pycom11g27740

Replication protein A interacting middle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0019180
Physical Location & Seq
Forward (+)
29906547 .. 29911244
4698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
ATGATATTGTCAGATATTTTAAATCTTTATACTTATAATAATTCATTCTTTCTAGTTTTAGAAAGGGGAAGAGAACATAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCGCTCAGAAGGAAACGTGGAGAGGAGATAAAGCGGTGAGACAAAAGGAATTGGAGGGAGCAGCTGCGGCTGCGGAAGCGGTGAGACAAAAGGAAATGGAGGGAGCAGCCGCAGCCGCAGCAGAAGGGCGGCATCCTCTCAAACCCCATCCTCGTTTCAATAACCGTAACAGCTGGAAACATAAGCTGAGAGAGAACTGTTACAAAAGGGTTCGAGAAGACAGAATGCGGTTGCTTTGGAAGATGAGAATGCTGCCCACTCCACAAATTCTCAATCACATTCCAAATGATTTGATCGAATCTGAATTCCAGGACATAGTTTCTGCTGAATTGGAAAAAATTAAGGAATCGTCATTGGATGAAAATGTAAGAAGTTCAACATCTGTCCCTGAGGCCAATGATATGTTATGGGAATACGATGGCCTTACAAATGTATACCAAGGTGATTGTGAAGAGATATTGTTAGAAATGCAAAGGATCTTTTATGAAGACCTGACTATTCATCCAACTAGAAAAGAAGAAACAGAAATCTGTGTTGAAACATGGGAAGATGAAGAGGATGAGTATTTGTCCCAGGCAGTTTATGAGCATATGCAACTGAACGACAAGCAGATGCACAAGGAGGAAGTTTGGTGCCCCGTCTGTAAGCACGGAGTACTACAAGAGAATTACAATCGAATATACTGCACTCTATGCAAGGTTCAGCTCAACAAAGGCAATGAGGTTAATTTGGAAATATTGCGGGGCAGGCTAGCAGAAGTTCACACTGAGCATCTGGATCGATCTGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPA_interact_C RPA_interact_M RPA_interact_N

Protein Analysis

306

Amino Acids

36.23

Weight (kDa)

5.4

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPA_interact_N PF14766 94 - 124 7e-10 Replication protein A interacting N-terminal
RPA_interact_M PF14767 144 - 235 1.1e-23 Replication protein A interacting middle
RPA_interact_C PF14768 252 - 301 2.1e-10 Replication protein A interacting C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12760
malus_domestica MD03G1298900.v1.1 MD11G1318200.v1.1
prunus_persica Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1 Prupe.8G267300_v2.0.a1
pyrus_communis pycom03g23700 pycom11g27740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 756
AccI GTMKAC 1 cut(s) 558
AciI CCGC 9 cut(s) 158, 191, 197, 203, 234, 240, 253, 352, 865
AclWI GGATC 2 cut(s) 608, 909
AcsI RAATTY 2 cut(s) 390, 428
AfaI GTAC 1 cut(s) 780
AfeI AGCGCT 1 cut(s) 127
AgsI TTSAA 3 cut(s) 283, 501, 662
AjnI CCWGG 2 cut(s) 432, 696
AluBI AGCT 4 cut(s) 188, 297, 310, 829
AluI AGCT 4 cut(s) 188, 297, 310, 829
Alw26I GTCTC 2 cut(s) 157, 202
AlwI GGATC 2 cut(s) 608, 909
Aor51HI AGCGCT 1 cut(s) 127
AoxI GGCC 2 cut(s) 516, 544
ApeKI GCWGC 7 cut(s) 185, 188, 194, 230, 236, 242, 376
ApoI RAATTY 2 cut(s) 390, 428
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 172, 217, 578
AsuNHI GCTAGC 1 cut(s) 874
AxyI CCTNAGG 1 cut(s) 513
BaeGI GKGCMC 1 cut(s) 761
BanI GGYRCC 1 cut(s) 756
BbsI GAAGAC 2 cut(s) 348, 618
BbvI GCAGC 7 cut(s) 175, 181, 197, 242, 248, 254, 363
BccI CCATC 2 cut(s) 279, 536
BcgI CGANNNNNNTGC 4 cut(s) 789, 823, 884, 918
BciT130I CCWGG 2 cut(s) 434, 698
BcoDI GTCTC 2 cut(s) 157, 202
BfaI CTAG 3 cut(s) 53, 633, 875
BfoI RGCGCY 1 cut(s) 129
BmcAI AGTACT 1 cut(s) 780
Bme1390I CCNGG 2 cut(s) 434, 698
BmiI GGNNCC 1 cut(s) 758
BmrFI CCNGG 2 cut(s) 434, 698
BmsI GCATC 3 cut(s) 265, 726, 904
BmtI GCTAGC 1 cut(s) 878
BpiI GAAGAC 2 cut(s) 348, 618
Bsa29I ATCGAT 1 cut(s) 904
BsaJI CCNNGG 2 cut(s) 562, 696
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 847
BseBI CCWGG 2 cut(s) 434, 698
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 2 cut(s) 562, 696
BseGI GGATG 5 cut(s) 256, 271, 487, 625, 688
BseMI GCAATG 1 cut(s) 847
BseMII CTCAG 4 cut(s) 143, 302, 504, 882
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 761
BseXI GCAGC 7 cut(s) 175, 181, 197, 242, 248, 254, 363
BsgI GTGCAG 1 cut(s) 793
BshFI GGCC 2 cut(s) 518, 546
BshNI GGYRCC 1 cut(s) 756
BshVI ATCGAT 1 cut(s) 904
BslFI GGGAC 2 cut(s) 494, 679
BsmAI GTCTC 2 cut(s) 157, 202
BsmFI GGGAC 2 cut(s) 494, 679
BsmI GAATGC 2 cut(s) 354, 378
BsnI GGCC 2 cut(s) 518, 546
Bsp1286I GDGCHC 1 cut(s) 761
Bsp143I GATC 4 cut(s) 417, 600, 901, 905
BspACI CCGC 9 cut(s) 158, 191, 197, 203, 234, 240, 253, 352, 865
BspANI GGCC 2 cut(s) 518, 546
BspCNI CTCAG 4 cut(s) 142, 303, 505, 883
BspDI ATCGAT 1 cut(s) 904
BspLI GGNNCC 1 cut(s) 758
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 2 cut(s) 608, 909
BspT107I GGYRCC 1 cut(s) 756
BsrDI GCAATG 1 cut(s) 847
BssECI CCNNGG 2 cut(s) 562, 696
BssMI GATC 4 cut(s) 417, 600, 901, 905
BssNAI GTATAC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 562
Bst1107I GTATAC 1 cut(s) 559
Bst2UI CCWGG 2 cut(s) 434, 698
Bst4CI ACNGT 2 cut(s) 290, 323
Bst6I CTCTTC 3 cut(s) 64, 570, 672
BstAPI GCANNNNNTGC 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 872, 876
BstDEI CTNAG 4 cut(s) 129, 311, 513, 891
BstF5I GGATG 5 cut(s) 256, 271, 487, 625, 688
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 420, 603, 904, 908
BstMAI GTCTC 2 cut(s) 157, 202
BstMBI GATC 4 cut(s) 417, 600, 901, 905
BstMWI GCNNNNNNNGC 8 cut(s) 191, 194, 200, 236, 239, 242, 816, 871
BstNI CCWGG 2 cut(s) 434, 698
BstSCI CCNGG 2 cut(s) 432, 696
BstSLI GKGCMC 1 cut(s) 761
BstV1I GCAGC 7 cut(s) 175, 181, 197, 242, 248, 254, 363
BstV2I GAAGAC 2 cut(s) 348, 618
BstX2I RGATCY 1 cut(s) 600
BstYI RGATCY 1 cut(s) 600
BstZ17I GTATAC 1 cut(s) 559
Bsu15I ATCGAT 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 2 cut(s) 518, 546
BsuTUI ATCGAT 1 cut(s) 904
BtsCI GGATG 5 cut(s) 256, 271, 487, 625, 688
BtsIMutI CAGTG 1 cut(s) 888
Cac8I GCNNGC 2 cut(s) 872, 876
CfoI GCGC 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 904
Csp6I GTAC 1 cut(s) 779
CviAII CATG 1 cut(s) 666
CviQI GTAC 1 cut(s) 779
DdeI CTNAG 4 cut(s) 129, 311, 513, 891
DpnI GATC 4 cut(s) 419, 602, 903, 907
DpnII GATC 4 cut(s) 417, 600, 901, 905
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 64, 570, 672
EarI CTCTTC 3 cut(s) 64, 570, 672
Eco130I CCWWGG 1 cut(s) 562
Eco47III AGCGCT 1 cut(s) 127
Eco81I CCTNAGG 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 2 cut(s) 432, 696
EcoT14I CCWWGG 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 1 cut(s) 669
FaqI GGGAC 2 cut(s) 494, 679
FatI CATG 1 cut(s) 665
FauI CCCGC 1 cut(s) 858
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 558
FokI GGATG 5 cut(s) 243, 258, 494, 612, 695
FspBI CTAG 3 cut(s) 53, 633, 875
GlaI GCGC 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 2 cut(s) 518, 546
HhaI GCGC 1 cut(s) 128
Hin1II CATG 1 cut(s) 669
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 2 cut(s) 422, 470
HphI GGTGA 3 cut(s) 172, 217, 578
Hpy166II GTNNAC 2 cut(s) 559, 886
Hpy188I TCNGA 3 cut(s) 13, 132, 427
Hpy188III TCNNGA 2 cut(s) 338, 899
Hpy8I GTNNAC 2 cut(s) 559, 886
HpyAV CCTTC 2 cut(s) 127, 242
HpyCH4III ACNGT 2 cut(s) 290, 323
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 6 cut(s) 595, 718, 739, 810, 819, 911
HpyF10VI GCNNNNNNNGC 8 cut(s) 191, 194, 200, 236, 239, 242, 816, 871
HpyF3I CTNAG 4 cut(s) 129, 311, 513, 891
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 1 cut(s) 669
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 417, 600, 901, 905
LmnI GCTCC 2 cut(s) 182, 227
LpnPI CCDG 9 cut(s) 283, 419, 446, 525, 629, 683, 710, 856, 884
Lsp1109I GCAGC 7 cut(s) 175, 181, 197, 242, 248, 254, 363
LweI GCATC 3 cut(s) 265, 726, 904
MaeI CTAG 3 cut(s) 53, 633, 875
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 2 cut(s) 290, 323
MalI GATC 4 cut(s) 419, 602, 903, 907
MboI GATC 4 cut(s) 417, 600, 901, 905
MboII GAAGA 8 cut(s) 81, 353, 376, 587, 623, 653, 683, 689
MflI RGATCY 1 cut(s) 600
MhlI GDGCHC 1 cut(s) 761
MluCI AATT 9 cut(s) 40, 173, 390, 428, 452, 462, 790, 850, 913
MmeI TCCRAC 1 cut(s) 653
MnlI CCTC 9 cut(s) 140, 172, 217, 270, 285, 508, 673, 739, 838
MseI TTAA 3 cut(s) 20, 465, 849
MspA1I CMGCKG 2 cut(s) 188, 297
MspR9I CCNGG 2 cut(s) 434, 698
Mva1269I GAATGC 2 cut(s) 354, 378
MvaI CCWGG 2 cut(s) 434, 698
MwoI GCNNNNNNNGC 8 cut(s) 191, 194, 200, 236, 239, 242, 816, 871
NdeI CATATG 1 cut(s) 714
NdeII GATC 4 cut(s) 417, 600, 901, 905
NheI GCTAGC 1 cut(s) 874
NlaIII CATG 1 cut(s) 669
NlaIV GGNNCC 1 cut(s) 758
PctI GAATGC 2 cut(s) 354, 378
PfeI GAWTC 2 cut(s) 422, 470
PfoI TCCNGGA 1 cut(s) 432
PsiI TTATAA 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 432, 696
PspGI CCWGG 2 cut(s) 432, 696
PspN4I GGNNCC 1 cut(s) 758
PsuI RGATCY 1 cut(s) 600
PvuII CAGCTG 2 cut(s) 188, 297
RsaI GTAC 1 cut(s) 780
RsaNI GTAC 1 cut(s) 779
SaqAI TTAA 3 cut(s) 20, 465, 849
Sau3AI GATC 4 cut(s) 417, 600, 901, 905
ScaI AGTACT 1 cut(s) 780
ScrFI CCNGG 2 cut(s) 434, 698
SduI GDGCHC 1 cut(s) 761
SetI ASST 9 cut(s) 143, 190, 299, 312, 568, 618, 825, 831, 849
SfaNI GCATC 3 cut(s) 265, 726, 904
Sse9I AATT 9 cut(s) 40, 173, 390, 428, 452, 462, 790, 850, 913
SsiI CCGC 9 cut(s) 158, 191, 197, 203, 234, 240, 253, 352, 865
SspI AATATT 1 cut(s) 861
SspMI CTAG 3 cut(s) 53, 633, 875
StyD4I CCNGG 2 cut(s) 432, 696
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 2 cut(s) 290, 323
TaiI ACGT 1 cut(s) 143
TaqI TCGA 4 cut(s) 337, 420, 799, 904
TasI AATT 9 cut(s) 40, 173, 390, 428, 452, 462, 790, 850, 913
TatI WGTACW 1 cut(s) 778
TauI GCSGC 4 cut(s) 194, 236, 242, 256
TfiI GAWTC 2 cut(s) 422, 470
Tru1I TTAA 3 cut(s) 20, 465, 849
Tru9I TTAA 3 cut(s) 20, 465, 849
TscAI CASTG 1 cut(s) 895
TseI GCWGC 7 cut(s) 185, 188, 194, 230, 236, 242, 376
TspDTI ATGAA 5 cut(s) 33, 498, 614, 624, 690
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 1 cut(s) 895
XapI RAATTY 2 cut(s) 390, 428
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 3 cut(s) 53, 633, 875
ZrmI AGTACT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.